Last update: Sat Aug 31 12:16:16 MDT 2024
Volume 188, Number 1, January, 2010Ben Short New function for an old master . . . . . ?? Ben Short Segregating out UbcH10's role in tumorigenesis . . . . . . . . . . . . . ?? Ben Short Synaptic vesicles are well connected . . ?? Ben Short The integrin reconstruction act . . . . 3 Kendall Powell Pier Paolo Di Fiore: Plumbing the depths of endocytosis . . . . . . . . . . . . . 4 Ramsey A. Saleem and John D. Aitchison Systems cell biology of the mitotic spindle . . . . . . . . . . . . . . . . 7 Peter Friedl and Katarina Wolf Plasticity of cell migration: a multiscale tuning model . . . . . . . . 11 John A. Follit and Lixia Li and Yvonne Vucica and Gregory J. Pazour The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence 21 Andrew M. Hudson and Lynn Cooley Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton . . . . . . . . . . . . . . 29 Keman Zhang and Jingfeng Sha and Marian L. Harter Activation of Cdc6 by MyoD is associated with the expansion of quiescent myogenic satellite cells . . . . . . . . . . . . 39 Gul Civelekoglu-Scholey and Li Tao and Ingrid Brust-Mascher and Roy Wollman and Jonathan M. Scholey Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin--B envelope . . . . . . . . . . . 49 Franco J. Vizeacoumar and Nydia van Dyk and Frederick S. Vizeacoumar and Vincent Cheung and Jingjing Li and Yaroslav Sydorskyy and Nicolle Case and Zhijian Li and Alessandro Datti and Corey Nislow and Brian Raught and Zhaolei Zhang and Brendan Frey and Kerry Bloom and Charles Boone and Brenda J. Andrews Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis . . . . . . . . . . . . . 69 Janine H. van Ree and Karthik B. Jeganathan and Liviu Malureanu and Jan M. van Deursen Overexpression of the E2 ubiquitin--conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation . . . . . . . . . . . . 83 Wei-Lien Yen and Takahiro Shintani and Usha Nair and Yang Cao and Brian C. Richardson and Zhijian Li and Frederick M. Hughson and Misuzu Baba and Daniel J. Klionsky The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy . . . . . . . 101 Yunling Xu and Li Yuan and Judy Mak and Luc Pardanaud and Maresa Caunt and Ian Kasman and Bruno Larrivée and Raquel del Toro and Steven Suchting and Alexander Medvinsky and Jillian Silva and Jian Yang and Jean-Léon Thomas and Alexander W. Koch and Kari Alitalo and Anne Eichmann and Anil Bagri Neuropilin-2 mediates VEGF--C--induced lymphatic sprouting together with VEGFR3 115 Christopher Esk and Chih-Ying Chen and Ludger Johannes and Frances M. Brodsky The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step . . . . . . . . . . . . . . . . . . 131 Rubén Fernández-Busnadiego and Beno\^\it Zuber and Ulrike Elisabeth Maurer and Marek Cyrklaff and Wolfgang Baumeister and Vladan Lu\vci\'c Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography . . . . . . . . . . . . . . . 145 Feng Ye and Guiqing Hu and Dianne Taylor and Boris Ratnikov and Andrey A. Bobkov and Mark A. McLean and Stephen G. Sligar and Kenneth A. Taylor and Mark H. Ginsberg Recreation of the terminal events in physiological integrin activation . . . 157
Mitch Leslie Cdks set the replication schedule . . . ?? Mitch Leslie Misfolded proteins go their separate ways . . . . . . . . . . . . . . . . . . ?? Mitch Leslie FYCO1 gets autophagy on track . . . . . ?? Mitch Leslie An enzyme with self-control . . . . . . 177 Ben Short Kenneth Yamada: Exploring the paths of cell migration . . . . . . . . . . . . . 178 Benedicte Delaval and Stephen J. Doxsey Pericentrin in cellular function and disease . . . . . . . . . . . . . . . . 181 Andrew J. Holland and Weijie Lan and Sherry Niessen and Heather Hoover and Don W. Cleveland Polo-like kinase 4 kinase activity limits centrosome overduplication by autoregulating its own stability . . . . 191 Jeong Su Oh and Seung Jin Han and Marco Conti Wee1B, Myt1, and Cdc25 function in distinct compartments of the mouse oocyte to control meiotic resumption . . 199 Alexander M. Thomson and Peter J. Gillespie and J. Julian Blow Replication factory activation can be decoupled from the replication timing program by modulating Cdk levels . . . . 209 Riccardo Bernasconi and Carmela Galli and Verena Calanca and Toshihiro Nakajima and Maurizio Molinari Stringent requirement for HRD1, SEL1L, and OS-9/XTP3-B for disposal of ERAD--L$_S$ substrates . . . . . . . . . 223 Yi Xiang and Yanzhuang Wang GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking . . . . . . . . . . . . . . . . 237 Serhiy Pankiv and Endalkachew A. Alemu and Andreas Brech and Jack-Ansgar Bruun and Trond Lamark and Aud Òvervatn and Geir Bjòrkòy and Terje Johansen FYCO1 is a Rab7 effector that binds to LC3 and PI3P to mediate microtubule plus end--directed vesicle transport . . . . 253 Armela Joset and Dana A. Dodd and Simon Halegoua and Martin E. Schwab Pincher-generated Nogo--A endosomes mediate growth cone collapse and retrograde signaling . . . . . . . . . . 271 Maxime F. Fournier and Roger Sauser and Davide Ambrosi and Jean-Jacques Meister and Alexander B. Verkhovsky Force transmission in migrating cells 287
Ben Short EGF takes dual control of mRNA . . . . . ?? Ben Short A SOLO performance in meiotic cohesion ?? Ben Short Identifying the prime suspects in vesicle release . . . . . . . . . . . . ?? Ben Short p53 hides a multitude of CIN . . . . . . 301 Nicole LeBrasseur Wolf Reik: Inheritance beyond DNA . . . 302 S. Y. Christin Chong and Jonah R. Chan Tapping into the glial reservoir: cells committed to remaining uncommitted . . . 305 Naomi R. Stevens and Jeroen Dobbelaere and Kathrin Brunk and Anna Franz and Jordan W. Raff Drosophila Ana2 is a conserved centriole duplication factor . . . . . . . . . . . 313 Nien-Pei Tsai and Ya-Lun Lin and Yao-Chen Tsui and Li-Na Wei Dual action of epidermal growth factor: extracellular signal-stimulated nuclear--cytoplasmic export and coordinated translation of selected messenger RNA . . . . . . . . . . . . . 325 Rihui Yan and Sharon E. Thomas and Jui-He Tsai and Yukihiro Yamada and Bruce D. McKee SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in \bionameDrosophila melanogaster . . . . . . . . . . . . . . 335 Cornelia König and Hiromi Maekawa and Elmar Schiebel Mutual regulation of cyclin-dependent kinase and the mitotic exit network . . 351 Sarah L. Thompson and Duane A. Compton Proliferation of aneuploid human cells is limited by a p53-dependent mechanism 369 András Masszi and Pam Speight and Emmanuel Charbonney and Monika Lodyga and Hiroyasu Nakano and Katalin Szászi and András Kapus Fate-determining mechanisms in epithelial--myofibroblast transition: major inhibitory role for Smad3 . . . . 383 Alexander M. Walter and Katrin Wiederhold and Dieter Bruns and Dirk Fasshauer and Jakob B. Sòrensen Synaptobrevin N-terminally bound to syntaxin--SNAP--25 defines the primed vesicle state in regulated exocytosis 401 Arun Anantharam and Bibiana Onoa and Robert H. Edwards and Ronald W. Holz and Daniel Axelrod Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM . . . . . 415 Atsushi Tamada and Satoshi Kawase and Fujio Murakami and Hiroyuki Kamiguchi Autonomous right-screw rotation of growth cone filopodia drives neurite turning . . . . . . . . . . . . . . . . 429 Sairei So and Anthony J. Davis and David J. Chen Autophosphorylation at serine 1981 stabilizes ATM at DNA damage sites . . . 443
Ben Short Plakophilin 1 is found in translation ?? Ben Short p53 strands cell cycle on archipelago ?? Ben Short Unpacking condensins' function in ES cells . . . . . . . . . . . . . . . . . ?? Ben Short Glycosphingolipids keep signaling in top--Notch condition . . . . . . . . . . 447 Caitlin Sedwick Graham Warren: Gaining ground on the Golgi . . . . . . . . . . . . . . . . . 448 Suzanne R. Pfeffer Unconventional secretion by autophagosome exocytosis . . . . . . . . 451 Christian Conrad and Daniel W. Gerlich Automated microscopy for high-content RNAi screening . . . . . . . . . . . . . 453 Annika Wolf and Malgorzata Krause-Gruszczynska and Olaf Birkenmeier and Antje Ostareck-Lederer and Stefan Hüttelmaier and Mechthild Hatzfeld Plakophilin 1 stimulates translation by promoting eIF4A1 activity . . . . . . . 463 Sudip Mandal and William A. Freije and Preeta Guptan and Utpal Banerjee Metabolic control of G1--S transition: cyclin E degradation by p53-induced activation of the ubiquitin--proteasome system . . . . . . . . . . . . . . . . . 473 Jesse C. Gatlin and Alexandre Matov and Gaudenz Danuser and Timothy J. Mitchison and Edward D. Salmon Directly probing the mechanical properties of the spindle and its matrix 481 Thomas G. Fazzio and Barbara Panning Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells . . . 491 Sebastian Deeg and Mathias Gralle and Kamila Sroka and Mathias Bähr and Fred Silvester Wouters and Pawel Kermer BAG1 restores formation of functional DJ-1 L166P dimers and DJ-1 chaperone activity . . . . . . . . . . . . . . . . 505 Neena S. Rane and Oishee Chakrabarti and Lionel Feigenbaum and Ramanujan S. Hegde Signal sequence insufficiency contributes to neurodegeneration caused by transmembrane prion protein . . . . . 515 Juan M. Duran and Christophe Anjard and Chris Stefan and William F. Loomis and Vivek Malhotra Unconventional secretion of Acb1 is mediated by autophagosomes . . . . . . . 527 Ravi Manjithaya and Christophe Anjard and William F. Loomis and Suresh Subramani Unconventional secretion of Pichia pastoris Acb1 is dependent on GRASP protein, peroxisomal functions, and autophagosome formation . . . . . . . . 537 Mirkka Koivusalo and Christopher Welch and Hisayoshi Hayashi and Cameron C. Scott and Moshe Kim and Todd Alexander and Nicolas Touret and Klaus M. Hahn and Sergio Grinstein Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling . . . . . . . . 547 David Owald and Wernher Fouquet and Manuela Schmidt and Carolin Wichmann and Sara Mertel and Harald Depner and Frauke Christiansen and Christina Zube and Christine Quentin and Jorg Körner and Henning Urlaub and Karl Mechtler and Stephan J. Sigrist A Syd-1 homologue regulates pre- and postsynaptic maturation in \bionameDrosophila . . . . . . . . . . . 565 Sophie Hamel and Jacques Fantini and François Schweisguth Notch ligand activity is modulated by glycosphingolipid membrane composition in \bionameDrosophila melanogaster . . . 581 Tom T. Chen and Alfonso Luque and Sunyoung Lee and Sean M. Anderson and Tatiana Segura and M. Luisa Iruela-Arispe Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells . . . . . 595 Zhenjie Xu and Hiromi Ogawa and Paola Vagnarelli and Jan H. Bergmann and Damien F. Hudson and Sandrine Ruchaud and Tatsuo Fukagawa and William C. Earnshaw and Kumiko Samejima INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization . . . . . . . . . . 611
Mitch Leslie Tangled DNA tightens chromosomes . . . . ?? Mitch Leslie SENP6 cuts SUMOs down to size . . . . . ?? Mitch Leslie Neighbors limit cell renovations . . . . ?? Mitch Leslie Centrosomes remember which way is up . . 615 Caitlin Sedwick Isabelle Vernos: Motoring around the mitotic spindle . . . . . . . . . . . . 616 Tom Misteli New editorial board members 2010 . . . . 619 Christine Sütterlin and Antonino Colanzi The Golgi and the centrosome: building a functional partnership . . . . . . . . . 621 Halfdan Beck and Viola Nähse and Marie Sofie Yoo Larsen and Petra Groth and Trevor Clancy and Michael Lees and Mette Jòrgensen and Thomas Helleday and Randi G. Syljuåsen and Claus Storgaard Sòrensen Regulators of cyclin-dependent kinases are crucial for maintaining genome integrity in S phase . . . . . . . . . . 629 Carolyn J. McNees and Agueda M. Tejera and Paula Martínez and Matilde Murga and Francisca Mulero and Oscar Fernandez-Capetillo and Maria A. Blasco ATR suppresses telomere fragility and recombination but is dispensable for elongation of short telomeres by telomerase . . . . . . . . . . . . . . . 639 Ryo Kawamura and Lisa H. Pope and Morten O. Christensen and Mingxuan Sun and Ksenia Terekhova and Fritz Boege and Christian Mielke and Anni H. Andersen and John F. Marko Mitotic chromosomes are constrained by topoisomerase II--sensitive DNA entanglements . . . . . . . . . . . . . 653 Khuloud Jaqaman and Emma M. King and Ana C. Amaro and Jennifer R. Winter and Jonas F. Dorn and Hunter L. Elliott and Nunu Mchedlishvili and Sarah E. McClelland and Iain M. Porter and Markus Posch and Alberto Toso and Gaudenz Danuser and Andrew D. McAinsh and Patrick Meraldi and Jason R. Swedlow Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases . . . . . . . . . . . . . 665 Debaditya Mukhopadhyay and Alexei Arnaoutov and Mary Dasso The SUMO protease SENP6 is essential for inner kinetochore assembly . . . . . . . 681 Jens Januschke and Cayetano Gonzalez The interphase microtubule aster is a determinant of asymmetric division orientation in \bionameDrosophila neuroblasts . . . . . . . . . . . . . . 693 Kazue Kanehara and Wei Xie and Davis T. W. Ng Modularity of the Hrd1 ERAD complex underlies its diverse client range . . . 707 Sungsu Kim and Yogesh P. Wairkar and Richard W. Daniels and Aaron DiAntonio The novel endosomal membrane protein Ema interacts with the class C Vps--HOPS complex to promote endosomal maturation 717 Adam C. Martin and Michael Gelbart and Rodrigo Fernandez-Gonzalez and Matthias Kaschube and Eric F. Wieschaus Integration of contractile forces during tissue invagination . . . . . . . . . . 735
Ben Short A team of rivals at the kinetochore . . ?? Ben Short Disuse TWEAKs muscle loss . . . . . . . ?? Ben Short Arf and Miz1 cause cells to lose their grip . . . . . . . . . . . . . . . . . . ?? Ben Short Actin oligomers hit the assembly line 753 Caitlin Sedwick Doug Hilton: At home with blood cell biology . . . . . . . . . . . . . . . . 754 Carolyn S. Sevier New insights into oxidative folding . . 757 Erkki Ruoslahti and Sangeeta N. Bhatia and Michael J. Sailor Targeting of drugs and nanoparticles to tumors . . . . . . . . . . . . . . . . . 759 Voytek Okreglak and David G. Drubin Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway . . 769 J. Kim Holloway and Meisha A. Morelli and Peter L. Borst and Paula E. Cohen Mammalian BLM helicase is critical for integrating multiple pathways of meiotic recombination . . . . . . . . . . . . . 779 Tomomi Kiyomitsu and Osamu Iwasaki and Chikashi Obuse and Mitsuhiro Yanagida Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes . . . . . . . . . . . . . . 791 Dan Liu and Mathijs Vleugel and Chelsea B. Backer and Tetsuya Hori and Tatsuo Fukagawa and Iain M. Cheeseman and Michael A. Lampson Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase . . . . 809 Ester Zito and King-Tung Chin and Jaime Blais and Heather P. Harding and David Ron ERO1-$ \beta $, a pancreas-specific disulfide oxidase, promotes insulin biogenesis and glucose homeostasis . . . 821 Ashwani Mittal and Shephali Bhatnagar and Akhilesh Kumar and Estelle Lach-Trifilieff and Sandrine Wauters and Hong Li and Denys Y. Makonchuk and David J. Glass and Ashok Kumar The TWEAK--Fn14 system is a critical regulator of denervation-induced skeletal muscle atrophy in mice . . . . 833 Bernhard Gillissen and Jana Wendt and Antje Richter and Anja Richter and Annika Müer and Tim Overkamp and Nina Gebhardt and Robert Preissner and Claus Belka and Bernd Dörken and Peter T. Daniel Endogenous Bak inhibitors Mcl-1 and Bcl-x$_L$: differential impact on TRAIL resistance in Bax-deficient carcinoma 851 Robert J. Cain and Bart Vanhaesebroeck and Anne J. Ridley The PI3K p110$ \alpha $ isoform regulates endothelial adherens junctions via Pyk2 and Rac1 . . . . . . . . . . . 863 Ana M. Pasapera and Ian C. Schneider and Erin Rericha and David D. Schlaepfer and Clare M. Waterman Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation 877 Janet A. Askari and Christopher J. Tynan and Stephen E. D. Webb and Marisa L. Martin-Fernandez and Christoph Ballestrem and Martin J. Humphries Focal adhesions are sites of integrin extension . . . . . . . . . . . . . . . 891 Barbara Herkert and Anne Dwertmann and Steffi Herold and Mona Abed and Jean-Francois Naud and Florian Finkernagel and Gregory S. Harms and Amir Orian and Michael Wanzel and Martin Eilers The Arf tumor suppressor protein inhibits Miz1 to suppress cell adhesion and induce apoptosis . . . . . . . . . . 905 Marcel Mettlen and Dinah Loerke and Defne Yarar and Gaudenz Danuser and Sandra L. Schmid Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis 919 Jingxuan Yang and Michael Meyer and Anna-Katharina Müller and Friederike Böhm and Richard Grose and Tina Dauwalder and Francois Verrey and Manfred Kopf and Juha Partanen and Wilhelm Bloch and David M. Ornitz and Sabine Werner Fibroblast growth factor receptors 1 and 2 in keratinocytes control the epidermal barrier and cutaneous homeostasis . . . 935 Sebiha Cevik and Yuji Hori and Oktay I. Kaplan and Katarzyna Kida and Tiina Toivenon and Christian Foley-Fisher and David Cottell and Toshiaki Katada and Kenji Kontani and Oliver E. Blacque Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in \bionameCaenorhabditis elegans . . . . . . . . . . . . . . . . 953
David D. Sabatini Philip Siekevitz: Bridging biochemistry and cell biology . . . . . . . . . . . . 3 Mitch Leslie Smooth muscle cells put their best podosome forward . . . . . . . . . . . . ?? Mitch Leslie Ataxia's double hit . . . . . . . . . . ?? Mitch Leslie IFT proteins go off the rails . . . . . ?? Mitch Leslie Endocytosis takes occludin for a ride 7 Ben Short Daniel Klionsky: a full plate for autophagy . . . . . . . . . . . . . . . 8 Katrin Karbstein Chaperoning ribosome assembly . . . . . 11 Manuela Quintavalle and Leonardo Elia and Gianluigi Condorelli and Sara A. Courtneidge MicroRNA control of podosome formation in vascular smooth muscle cells in vivo and in vitro . . . . . . . . . . . . . . 13 Alexis R. Barr and John V. Kilmartin and Fanni Gergely CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response . . . . . . . . . . . . . . . . 23 Yasuhiro Araki and Linda Gombos and Suellen P. S. Migueleti and Lavanya Sivashanmugam and Claude Antony and Elmar Schiebel N-terminal regions of Mps1 kinase determine functional bifurcation . . . . 41 Ansgar Koplin and Steffen Preissler and Yulia Ilina and Miriam Koch and Annika Scior and Marc Erhardt and Elke Deuerling A dual function for chaperones SSB--RAC and the NAC nascent polypeptide--associated complex on ribosomes . . . . . . . . . . . . . . . 57 Véronique Alban\`ese and Stefanie Reissmann and Judith Frydman A ribosome-anchored chaperone network that facilitates eukaryotic ribosome biogenesis . . . . . . . . . . . . . . . 69 Caoimhín G. Concannon and Liam P. Tuffy and Petronela Weisová and Helena P. Bonner and David Dávila and Caroline Bonner and Marc C. Devocelle and Andreas Strasser and Manus W. Ward and Jochen H. M. Prehn AMP kinase-mediated activation of the BH3-only protein Bim couples energy depletion to stress-induced apoptosis 83 David S. Gokhin and Raymond A. Lewis and Caroline R. McKeown and Roberta B. Nowak and Nancy E. Kim and Ryan S. Littlefield and Richard L. Lieber and Velia M. Fowler Tropomodulin isoforms regulate thin filament pointed-end capping and skeletal muscle physiology . . . . . . . 95 Amanda M. Marchiando and Le Shen and W. Vallen Graham and Christopher R. Weber and Brad T. Schwarz and Jotham R. Austin and David R. Raleigh and Yanfang Guan and Alastair J. M. Watson and Marshall H. Montrose and Jerrold R. Turner Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo . . . 111 Keith E. Szulwach and Xuekun Li and Richard D. Smrt and Yujing Li and Yuping Luo and Li Lin and Nicholas J. Santistevan and Wendi Li and Xinyu Zhao and Peng Jin Cross talk between microRNA and epigenetic regulation in adult neurogenesis . . . . . . . . . . . . . . 127 Damaris N. Lorenzo and Min-gang Li and Sarah E. Mische and Karen R. Armbrust and Laura P. W. Ranum and Thomas S. Hays Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in \bionameDrosophila 143 Jun Kohyama and Tsukasa Sanosaka and Akinori Tokunaga and Eriko Takatsuka and Keita Tsujimura and Hideyuki Okano and Kinichi Nakashima BMP-induced REST regulates the establishment and maintenance of astrocytic identity . . . . . . . . . . 159 Tina Sedmak and Uwe Wolfrum Intraflagellar transport molecules in ciliary and nonciliary cells of the retina . . . . . . . . . . . . . . . . . 171 Sebiha Cevik and Yuji Hori and Oktay I. Kaplan and Katarzyna Kida and Tiina Toivenon and Christian Foley-Fisher and David Cottell and Toshiaki Katada and Kenji Kontani and Oliver E. Blacque Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in \bionameCaenorhabditis elegans . . . . . . . . . . . . . . . . 187
Ben Short Rab35 drives exosome secretion . . . . . ?? Ben Short Epithelial cells on death Rho . . . . . ?? Ben Short MIM gives cells a sense of direction . . ?? Ben Short Deciding what to eat . . . . . . . . . . 191 Caitlin Sedwick Laurie Glimcher: Merging cell biology and immune function . . . . . . . . . . 192 Mike Rossner New style, same substance . . . . . . . 195 Arne Lindqvist Cyclin B--Cdk1 activates its own pump to get into the nucleus . . . . . . . . . . 197 Tania Bose and Jennifer L. Gerton Cohesinopathies, gene expression, and chromatin organization . . . . . . . . . 201 Noriyuki Matsuda and Shigeto Sato and Kahori Shiba and Kei Okatsu and Keiko Saisho and Clement A. Gautier and Yu-shin Sou and Shinji Saiki and Sumihiro Kawajiri and Fumiaki Sato and Mayumi Kimura and Masaaki Komatsu and Nobutaka Hattori and Keiji Tanaka PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy . . . . . . 211 Chieh Hsu and Yuichi Morohashi and Shin-ichiro Yoshimura and Natalia Manrique-Hoyos and SangYong Jung and Marcel A. Lauterbach and Mostafa Bakhti and Mads Grònborg and Wiebke Möbius and JeongSeop Rhee and Francis A. Barr and Mikael Simons Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A--C . . . . . . . . . . . . . . 223 Christopher Van and Shan Yan and W. Matthew Michael and Shou Waga and Karlene A. Cimprich Continued primer synthesis at stalled replication forks contributes to checkpoint activation . . . . . . . . . 233 Olivier Gavet and Jonathon Pines Activation of cyclin B1--Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis . . . . . . . . 247 Alexander E. Davies and Kenneth B. Kaplan Hsp90--Sgt1 and Skp1 target human Mis12 complexes to ensure efficient formation of kinetochore--microtubule binding sites . . . . . . . . . . . . . . . . . 261 Zhen Zheng and Huabin Zhu and Qingwen Wan and Jing Liu and Zhuoni Xiao and David P. Siderovski and Quansheng Du LGN regulates mitotic spindle orientation during epithelial morphogenesis . . . . . . . . . . . . . 275 Gabrielle Haas and Joerg E. Braun and Cátia Igreja and Felix Tritschler and Tadashi Nishihara and Elisa Izaurralde HPat provides a link between deadenylation and decapping in metazoa 289 Ravi Manjithaya and Shveta Jain and Jean-Claude Farré and Suresh Subramani A yeast MAPK cascade regulates pexophagy but not other autophagy pathways . . . . 303 Amanda L. Neisch and Olga Speck and Beth Stronach and Richard G. Fehon Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane . . . . . . . . . . . . 311 Kohei Yamamizu and Taichi Matsunaga and Hideki Uosaki and Hiroyuki Fukushima and Shiori Katayama and Mina Hiraoka-Kanie and Kohnosuke Mitani and Jun K. Yamashita Convergence of Notch and $ \beta $-catenin signaling induces arterial fate in vascular progenitors . . . . . . 325 Jacqueline M. Benjamin and Adam V. Kwiatkowski and Changsong Yang and Farida Korobova and Sabine Pokutta and Tatyana Svitkina and William I. Weis and W. James Nelson $ \alpha $E-catenin regulates actin dynamics independently of cadherin-mediated cell--cell adhesion 339 Gabriel A. Quinones and Janet Jin and Anthony E. Oro I-BAR protein antagonism of endocytosis mediates directional sensing during guided cell migration . . . . . . . . . 353 Daniel C. Worth and Kairbaan Hodivala-Dilke and Stephen D. Robinson and Samantha J. King and Penny E. Morton and Frank B. Gertler and Martin J. Humphries and Maddy Parsons $ \alpha $ v$ \beta $3 integrin spatially regulates VASP and RIAM to control adhesion dynamics and migration 369 Mirkka Koivusalo and Christopher Welch and Hisayoshi Hayashi and Cameron C. Scott and Moshe Kim and Todd Alexander and Nicolas Touret and Klaus M. Hahn and Sergio Grinstein Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling . . . . . . . . 385
Mitch Leslie Unfaithful proteins no problem for DNA repair . . . . . . . . . . . . . . . . . ?? Mitch Leslie Skeleton key for metastasis . . . . . . ?? Mitch Leslie RALT doubles down on EGFR . . . . . . . ?? Mitch Leslie Peter Pan bone cells undermine skeleton 389 Ben Short Christine Jacobs--Wagner: Drawing the bacterial organizational chart . . . . . 390 Mike Rossner Updating realistic access . . . . . . . 393 Jyothi Arikkath N-cadherin: stabilizing synapses . . . . 397 Gillian M. Griffiths and Andy Tsun and Jane C. Stinchcombe The immunological synapse: a focal point for endocytosis and exocytosis . . . . . 399 Yupeng Zheng and Sam John and James J. Pesavento and Jennifer R. Schultz-Norton and R. Louis Schiltz and Sonjoon Baek and Ann M. Nardulli and Gordon L. Hager and Neil L. Kelleher and Craig A. Mizzen Histone H1 phosphorylation is associated with transcription by RNA polymerases I and II . . . . . . . . . . . . . . . . . 407 Li Chen and Annalisa Mupo and Tuong Huynh and Sara Cioffi and Matthew Woods and Chengliu Jin and Wallace McKeehan and LuAnn Thompson-Snipes and Antonio Baldini and Elizabeth Illingworth Tbx1 regulates Vegfr3 and is required for lymphatic vessel development . . . . 417 Yasushi Enokido and Takuya Tamura and Hikaru Ito and Anup Arumughan and Akihiko Komuro and Hiroki Shiwaku and Masaki Sone and Raphaele Foulle and Hirohide Sawada and Hiroshi Ishiguro and Tetsuya Ono and Miho Murata and Ichiro Kanazawa and Nikolai Tomilin and Kazuhiko Tagawa and Erich E. Wanker and Hitoshi Okazawa Mutant huntingtin impairs Ku70-mediated DNA repair . . . . . . . . . . . . . . . 425 Martijn S. Luijsterburg and Gesa von Bornstaedt and Audrey M. Gourdin and Antonio Z. Politi and Martijn J. Moné and Daniël O. Warmerdam and Joachim Goedhart and Wim Vermeulen and Roel van Driel and Thomas Höfer Stochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair . . . . . . . . . . . . 445 Christian Hentrich and Thomas Surrey Microtubule organization by the antagonistic mitotic motors kinesin-5 and kinesin-14 . . . . . . . . . . . . . 465 Lori E. Krueger and Jui-Ching Wu and Meng-Fu Bryan Tsou and Lesilee S. Rose LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in \bionameC. elegans embryos 481 Alexander Heuck and Ingrid Fetka and Daniel N. Brewer and Daniela Hüls and Mary Munson and Ralf-Peter Jansen and Dierk Niessing The structure of the Myo4p globular tail and its function in ASH1 mRNA localization . . . . . . . . . . . . . . 497 Michael L. Sohaskey and Yebin Jiang and Jenny J. Zhao and Andreas Mohr and Frank Roemer and Richard M. Harland Osteopotentia regulates osteoblast maturation, bone formation, and skeletal integrity in mice . . . . . . . . . . . 511 Christelle Etard and Urmas Roostalu and Uwe Strähle Lack of Apobec2-related proteins causes a dystrophic muscle phenotype in zebrafish embryos . . . . . . . . . . . 527 Marie Schoumacher and Robert D. Goldman and Daniel Louvard and Danijela M. Vignjevic Actin, microtubules, and vimentin intermediate filaments cooperate for elongation of invadopodia . . . . . . . 541 Yuri Frosi and Sergio Anastasi and Costanza Ballar\`o and Giulia Varsano and Loriana Castellani and Elena Maspero and Simona Polo and Stefano Alem\`a and Oreste Segatto A two-tiered mechanism of EGFR inhibition by RALT/MIG6 via kinase suppression and receptor degradation . . 557 Supachoke Mangmool and Arun K. Shukla and Howard A. Rockman $ \beta $-Arrestin-dependent activation of Ca$^{2+}$ /calmodulin kinase II after $ \beta_1$ --adrenergic receptor stimulation . . . . . . . . . . . . . . 573 Pablo Mendez and Mathias De Roo and Lorenzo Poglia and Paul Klauser and Dominique Muller N-cadherin mediates plasticity-induced long-term spine stabilization . . . . . 589 Christen G. DiPetrillo and Elizabeth F. Smith Pcdp1 is a central apparatus protein that binds Ca$^{2+}$-calmodulin and regulates ciliary motility . . . . . . . 601
Ben Short Cdc14 in good repair . . . . . . . . . . ?? Ben Short Parkin restrictions for damaged mitochondria . . . . . . . . . . . . . . ?? Ben Short A turning point for macrophages . . . . ?? Ben Short Kinetochores hang on for the ride . . . 615 Caitlin Sedwick Andy Dillin: Using aging research to probe biology . . . . . . . . . . . . . 616 Pirta Hotulainen and Casper C. Hoogenraad Actin in dendritic spines: connecting dynamics to function . . . . . . . . . . 619 Annamaria Mocciaro and Eli Berdougo and Kang Zeng and Elizabeth Black and Paola Vagnarelli and William Earnshaw and David Gillespie and Prasad Jallepalli and Elmar Schiebel Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair . . . . . . . . . . . . . 631 Fabienne Lampert and Peter Hornung and Stefan Westermann The Dam1 complex confers microtubule plus end--tracking activity to the Ndc80 kinetochore complex . . . . . . . . . . 641 Pierre Romé and Emilie Montembault and Nathalie Franck and Aude Pascal and David M. Glover and Régis Giet Aurora A contributes to p150$^{\rm glued}$ phosphorylation and function during mitosis . . . . . . . . . . . . . 651 Yi Qin and Walter H. Meisen and Yi Hao and Ian G. Macara Tuba, a Cdc42 GEF, is required for polarized spindle orientation during epithelial cyst formation . . . . . . . 661 Joo-Yong Lee and Yoshito Nagano and J. Paul Taylor and Kah Leong Lim and Tso-Pang Yao Disease-causing mutations in Parkin impair mitochondrial ubiquitination, aggregation, and HDAC6-dependent mitophagy . . . . . . . . . . . . . . . 671 Brian Stramer and Severina Moreira and Tom Millard and Iwan Evans and Chieh-Yin Huang and Ola Sabet and Martin Milner and Graham Dunn and Paul Martin and Will Wood Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in \bionameDrosophila macrophages in vivo . . . . . . . . . . 681 Yo Sugawara and Takuhiro Matsumura and Yuki Takegahara and Yingji Jin and Yoshikazu Tsukasaki and Masatoshi Takeichi and Yukako Fujinaga Botulinum hemagglutinin disrupts the intercellular epithelial barrier by directly binding E-cadherin . . . . . . 691 Markus Finzsch and Silke Schreiner and Tatjana Kichko and Peter Reeh and Ernst R. Tamm and Michael R. Bösl and Dies Meijer and Michael Wegner Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage . . . . . . 701 Jerry F. Tien and Neil T. Umbreit and Daniel R. Gestaut and Andrew D. Franck and Jeremy Cooper and Linda Wordeman and Tamir Gonen and Charles L. Asbury and Trisha N. Davis Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B . . . . . . . . . . . . . . . . 713 Alejo E. Rodriguez-Fraticelli and Silvia Vergarajauregui and Dennis J. Eastburn and Anirban Datta and Miguel A. Alonso and Keith Mostov and Fernando Martín-Belmonte The Cdc42 GEF Intersectin 2 controls mitotic spindle orientation to form the lumen during epithelial morphogenesis 725 Nina C. Hubner and Alexander W. Bird and Jürgen Cox and Bianca Splettstoesser and Peter Bandilla and Ina Poser and Anthony Hyman and Matthias Mann Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions . . . . . . . . . . 739 Sunglan Chung and Peter A. Takizawa Multiple Myo4 motors enhance ASH1 mRNA transport in \bionameSaccharomyces cerevisiae . . . . . . . . . . . . . . . 755 Ester Zito and King-Tung Chin and Jaime Blais and Heather P. Harding and David Ron ERO1-$ \beta $, a pancreas-specific disulfide oxidase, promotes insulin biogenesis and glucose homeostasis . . . 769
Ben Short Stress management for mRNAs . . . . . . ?? Ben Short The story of O-glycosylation . . . . . . ?? Ben Short Nebulin doesn't measure up . . . . . . . ?? Ben Short The acid test of v--ATPase function . . 773 Caitlin Sedwick Inke Näthke: The ABCs of APC . . . . . . 774 Melissa Linkert and Curtis T. Rueden and Chris Allan and Jean-Marie Burel and Will Moore and Andrew Patterson and Brian Loranger and Josh Moore and Carlos Neves and Donald MacDonald and Aleksandra Tarkowska and Caitlin Sticco and Emma Hill and Mike Rossner and Kevin W. Eliceiri and Jason R. Swedlow Metadata matters: access to image data in the real world . . . . . . . . . . . 777 D. Thomas Rutkowski and Ramanujan S. Hegde Regulation of basal cellular physiology by the homeostatic unfolded protein response . . . . . . . . . . . . . . . . 783 Anne Chadrin and Barbara Hess and Mabel San Roman and Xavier Gatti and Bérang\`ere Lombard and Damarys Loew and Yves Barral and Benoit Palancade and Valérie Doye Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution . . . . . . . . . . . . . . 795 Je-Hyun Yoon and Eui-Ju Choi and Roy Parker Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in \bionameSaccharomyces cerevisiae . . 813 Bradley R. Pearse and Taku Tamura and Johan C. Sunryd and Gregory A. Grabowski and Randal J. Kaufman and Daniel N. Hebert The role of UDP--Glc: glycoprotein glucosyltransferase 1 in the maturation of an obligate substrate prosaposin . . 829 David J. Gill and Joanne Chia and Jamie Senewiratne and Frederic Bard Regulation of O-glycosylation through Golgi--to--ER relocation of initiation enzymes . . . . . . . . . . . . . . . . 843 Christopher T. Pappas and Paul A. Krieg and Carol C. Gregorio Nebulin regulates actin filament lengths by a stabilization mechanism . . . . . . 859 Lai Kuan Goh and Fangtian Huang and Woong Kim and Steven Gygi and Alexander Sorkin Multiple mechanisms collectively regulate clathrin-mediated endocytosis of the epidermal growth factor receptor 871 W. Ryan Williamson and Dong Wang and Adam S. Haberman and P. Robin Hiesinger A dual function of V0-ATPase a1 provides an endolysosomal degradation mechanism in \bionameDrosophila melanogaster photoreceptors . . . . . . . . . . . . . 885 Azusa Hotta and Tomomi Kawakatsu and Tomoya Nakatani and Toshitaka Sato and Chiyuki Matsui and Taiko Sukezane and Tsuyoshi Akagi and Tomoko Hamaji and Ilya Grigoriev and Anna Akhmanova and Yoshimi Takai and Yuko Mimori-Kiyosue Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5$ \alpha $ /$ \beta $ . . . . . . . . . . . . . . . 901
Ben Short Polyglutamylation makes the cut . . . . ?? Ben Short Deconstructing FAK function . . . . . . ?? Ben Short Acid sphingomyelinase deals the seal . . ?? Ben Short In search of lost timing . . . . . . . . 921 Caitlin Sedwick Joan Brugge: Running rings around cancer 922 Tatsuya Saitoh and Shizuo Akira Regulation of innate immune responses by autophagy-related proteins . . . . . . . 925 Katherine Johnston and Ajit Joglekar and Tetsuya Hori and Aussie Suzuki and Tatsuo Fukagawa and E. D. Salmon Vertebrate kinetochore protein architecture: protein copy number . . . 937 Benjamin Lacroix and Juliette van Dijk and Nicholas D. Gold and Julien Guizetti and Gudrun Aldrian-Herrada and Krzysztof Rogowski and Daniel W. Gerlich and Carsten Janke Tubulin polyglutamylation stimulates spastin-mediated microtubule severing 945 Xiaofeng Zhao and Xu Peng and Shaogang Sun and Ann Y. J. Park and Jun-Lin Guan Role of kinase-independent and -dependent functions of FAK in endothelial cell survival and barrier function during embryonic development 955 Junjie Lu and Feng Li and Christopher S. Murphy and Michael W. Davidson and David M. Gilbert G2 phase chromatin lacks determinants of replication timing . . . . . . . . . . . 967 Laura A. Buttitta and Alexia J. Katzaroff and Bruce A. Edgar A robust cell cycle control mechanism limits E2F-induced proliferation of terminally differentiated cells in vivo 981 Hesso Farhan and Markus W. Wendeler and Sandra Mitrovic and Eugenio Fava and Yael Silberberg and Roded Sharan and Marino Zerial and Hans-Peter Hauri MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screening . . . . . . . . . . 997 Jennetta W. Hammond and T. Lynne Blasius and Virupakshi Soppina and Dawen Cai and Kristen J. Verhey Autoinhibition of the kinesin-2 motor KIF17 via dual intramolecular mechanisms 1013 Christina Tam and Vincent Idone and Cecilia Devlin and Maria Cecilia Fernandes and Andrew Flannery and Xingxuan He and Edward Schuchman and Ira Tabas and Norma W. Andrews Exocytosis of acid sphingomyelinase by wounded cells promotes endocytosis and plasma membrane repair . . . . . . . . . 1027 Yujie Li and Qing Wei and Yuxia Zhang and Kun Ling and Jinghua Hu The small GTPases ARL-13 and ARL-3 coordinate intraflagellar transport and ciliogenesis . . . . . . . . . . . . . . 1039
Mitch Leslie Clathrin teams up to strengthen the spindle . . . . . . . . . . . . . . . . ?? Mitch Leslie Secret passage to the inner nuclear membrane . . . . . . . . . . . . . . . . ?? Mitch Leslie Lysosomes don't accentuate the negative ?? Mitch Leslie APC: More than a beta (catenin) blocker 1055 Ben Short Sean Morrison: a root and branch approach to stem cells . . . . . . . . . 1056 Michael R. Elliott and Kodi S. Ravichandran Clearance of apoptotic cells: implications in health and disease . . . 1059 Marlene Oeffinger Joining the interface: a site for Nmd3 association on 60S ribosome subunits . . 1071 Michael Smutny and Alpha S. Yap Neighborly relations: cadherins and mechanotransduction . . . . . . . . . . 1075 Jayati Sengupta and Cyril Bussiere and Jesper Pallesen and Matthew West and Arlen W. Johnson and Joachim Frank Characterization of the nuclear export adaptor protein Nmd3 in association with the 60S ribosomal subunit . . . . . . . 1079 Kyoko Okada and Francesca Bartolini and Alexandra M. Deaconescu and James B. Moseley and Zvonimir Dogic and Nikolaus Grigorieff and Gregg G. Gundersen and Bruce L. Goode Adenomatous polyposis coli protein nucleates actin assembly and synergizes with the formin mDia1 . . . . . . . . . 1087 Chiou-Hong Lin and Chi-Kuo Hu and Hsiu-Ming Shih Clathrin heavy chain mediates TACC3 targeting to mitotic spindles to ensure spindle stability . . . . . . . . . . . 1097 Quint le Duc and Quanming Shi and Iris Blonk and Arnoud Sonnenberg and Ning Wang and Deborah Leckband and Johan de Rooij Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner . . . . . . . . . . . . . . . . . 1107 Masaoki Kohzaki and Kana Nishihara and Kouji Hirota and Eiichiro Sonoda and Michio Yoshimura and Shigeo Ekino and John E. Butler and Masami Watanabe and Thanos D. Halazonetis and Shunichi Takeda DNA polymerases $ \nu $ and $ \theta $ are required for efficient immunoglobulin V gene diversification in chicken . . . . . . . . . . . . . . . . 1117 Gandhi Theerthagiri and Nathalie Eisenhardt and Heinz Schwarz and Wolfram Antonin The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex . . . . . . . . . . . . . . 1129 Christopher W. Carroll and Kirstin J. Milks and Aaron F. Straight Dual recognition of CENP--A nucleosomes is required for centromere assembly . . 1143 Yuting Sun and Yejing Ge and Jenny Drnevich and Yong Zhao and Mark Band and Jie Chen Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis . . . . . . . . . . . . . . . 1157 Benjamin E. Steinberg and Kassidy K. Huynh and Alexandre Brodovitch and Sabrina Jabs and Tobias Stauber and Thomas J. Jentsch and Sergio Grinstein A cation counterflux supports lysosomal acidification . . . . . . . . . . . . . 1171 Seung-Hwan Kim and Antonia H. Holway and Suzanne Wolff and Andrew Dillin and W. Matthew Michael SMK-1/PPH-4.1-mediated silencing of the CHK-1 response to DNA damage in early \bionameC. elegans embryos . . . . . . . 1187
Ben Short Long asters rock the spindle . . . . . . ?? Ben Short SPSB2 sets NO limits . . . . . . . . . . ?? Ben Short PIH proteins give dynein arms a hand . . ?? Ben Short Sar1 bends membranes into shape . . . . 3 Caitlin Sedwick David Pellman: Grasping the geometry of cancer . . . . . . . . . . . . . . . . . 4 Priya Prakash Budde and Tom Misteli Cell biology beyond the cell . . . . . . 7 Martin Schwander and Bechara Kachar and Ulrich Müller The cell biology of hearing . . . . . . 9 Weijie Lan and Don W. Cleveland A chemical tool box defines mitotic and interphase roles for Mps1 kinase . . . . 21 Laura Hewitt and Anthony Tighe and Stefano Santaguida and Anne M. White and Clifford D. Jones and Andrea Musacchio and Stephen Green and Stephen S. Taylor Sustained Mps1 activity is required in mitosis to recruit O--Mad2 to the Mad1--C--Mad2 core complex . . . . . . . 25 Kathleen E. Rankin and Linda Wordeman Long astral microtubules uncouple mitotic spindles from the cytokinetic furrow . . . . . . . . . . . . . . . . . 35 Marie E. Bechler and Anne M. Doody and Esther Racoosin and Lin Lin and Kelvin H. Lee and William J. Brown The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex . . . . . . . . . . . 45 Jingshi Shen and Shailendra S. Rathore and Lavan Khandan and James E. Rothman SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion 55 Ryosuke Yamamoto and Masafumi Hirono and Ritsu Kamiya Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins . . . . . . 65 Stefano Santaguida and Anthony Tighe and Anna Morena D'Alise and Stephen S. Taylor and Andrea Musacchio Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine . . . . . . . . . . 73 John Maciejowski and Kelly A. George and Marie-Emilie Terret and Chao Zhang and Kevan M. Shokat and Prasad V. Jallepalli Mps1 directs the assembly of Cdc20 inhibitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling . . . . 89 Poonam R. Molli and Da-Qiang Li and Rozita Bagheri-Yarmand and Suresh B. Pakala and Hiroshi Katayama and Subrata Sen and Jyoti Iyer and Jonathan Chernoff and Ming-Ying Tsai and Sujit S. Nair and Rakesh Kumar Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A 101 Kimberly R. Long and Yasunori Yamamoto and Adam L. Baker and Simon C. Watkins and Carolyn B. Coyne and James F. Conway and Meir Aridor Sar1 assembly regulates membrane constriction and ER export . . . . . . . 115 Zhihe Kuang and Rowena S. Lewis and Joan M. Curtis and Yifan Zhan and Bernadette M. Saunders and Jeffrey J. Babon and Tatiana B. Kolesnik and Andrew Low and Seth L. Masters and Tracy A. Willson and Lukasz Kedzierski and Shenggen Yao and Emanuela Handman and Raymond S. Norton and Sandra E. Nicholson The SPRY domain--containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation . . . . . . . . 129 Adrian Mehlitz and Sebastian Banhart and André P. Mäurer and Alexis Kaushansky and Andrew G. Gordus and Julia Zielecki and Gavin MacBeath and Thomas F. Meyer Tarp regulates early \bionameChlamydia-induced host cell survival through interactions with the human adaptor protein SHC1 . . . . . . . 143
Mitch Leslie Virus takes the actin express . . . . . ?? Mitch Leslie Mitosis first, DNA repair later . . . . ?? Mitch Leslie Ins and outs of Cdc14 . . . . . . . . . ?? Mitch Leslie Repetitive but not redundant . . . . . . 161 Caitlin Sedwick Sandra Schmid: Collaring endocytosis . . 162 Lothar Schermelleh and Rainer Heintzmann and Heinrich Leonhardt A guide to super-resolution fluorescence microscopy . . . . . . . . . . . . . . . 165 Emily A. Bassett and Stacey Wood and Kevan J. Salimian and Sandya Ajith and Daniel R. Foltz and Ben E. Black Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors . . . . . . . . . . . . . 177 Taro Ohkawa and Loy E. Volkman and Matthew D. Welch Actin-based motility drives baculovirus transit to the nucleus and cell surface 187 Simona Giunta and Rimma Belotserkovskaya and Stephen P. Jackson DNA damage signaling in response to double-strand breaks during mitosis . . 197 Romilde Manzoni and Francesca Montani and Clara Visintin and Fabrice Caudron and Andrea Ciliberto and Rosella Visintin Oscillations in Cdc14 release and sequestration reveal a circuit underlying mitotic exit . . . . . . . . 209 Shigeko Kawai-Noma and Chan-Gi Pack and Tomoko Kojidani and Haruhiko Asakawa and Yasushi Hiraoka and Masataka Kinjo and Tokuko Haraguchi and Hideki Taguchi and Aiko Hirata In vivo evidence for the fibrillar structures of Sup35 prions in yeast cells . . . . . . . . . . . . . . . . . 223 Huaqing Cai and Satarupa Das and Yoichiro Kamimura and Yu Long and Carole A. Parent and Peter N. Devreotes Ras-mediated activation of the TORC2--PKB pathway is critical for chemotaxis . . . . . . . . . . . . . . . 233 Wei-Ting Chao and Alexes C. Daquinag and Felicity Ashcroft and Jeannette Kunz Type I PIPK-$ \alpha $ regulates directed cell migration by modulating Rac1 plasma membrane targeting and activation . . . . . . . . . . . . . . . 247 Shengda Lin and Lisa M. Baye and Trudi A. Westfall and Diane C. Slusarski Wnt5b--Ryk pathway provides directional signals to regulate gastrulation movement . . . . . . . . . . . . . . . . 263
Ben Short $ \gamma $-Tubulin keeps the cell cycling . . . . . . . . . . . . . . . . ?? Ben Short Acetylated microtubules let the ER slide ?? Ben Short Syndecan-3 adds a Notch to its belt . . ?? Ben Short FLIPping the kill switch . . . . . . . . 281 Ben Short Yukiko Goda: Memories are made of this 282 Nirupa Chaudhari and Stephen D. Roper The cell biology of taste . . . . . . . 285 Naihan Xu and Nadia Hegarat and Elizabeth J. Black and Mary T. Scott and Helfrid Hochegger and David A. Gillespie Akt\slash PKB suppresses DNA damage processing and checkpoint activation in late G2 . . . . . . . . . . . . . . . . 297 Fubito Nakatsu and Rushika M. Perera and Louise Lucast and Roberto Zoncu and Jan Domin and Frank B. Gertler and Derek Toomre and Pietro De Camilli The inositol $5$-phosphatase SHIP2 regulates endocytic clathrin-coated pit dynamics . . . . . . . . . . . . . . . . 307 Tania Nayak and Heather Edgerton-Morgan and Tetsuya Horio and Yi Xiong and Colin P. De Souza and Stephen A. Osmani and Berl R. Oakley $ \gamma $-Tubulin regulates the anaphase-promoting complex/cyclosome during interphase . . . . . . . . . . . 317 Yan Shan Ong and Bor Luen Tang and Li Shen Loo and Wanjin Hong p125A exists as part of the mammalian Sec13/Sec31 COPII subcomplex to facilitate ER--Golgi transport . . . . . 331 James R. R. Whittle and Thomas U. Schwartz Structure of the Sec13--Sec16 edge element, a template for assembly of the COPII vesicle coat . . . . . . . . . . . 347 Jonathan R. Friedman and Brant M. Webster and David N. Mastronarde and Kristen J. Verhey and Gia K. Voeltz ER sliding dynamics and ER--mitochondrial contacts occur on acetylated microtubules . . . . . . . . 363 Nicolai Fricker and Joel Beaudouin and Petra Richter and Roland Eils and Peter H. Krammer and Inna N. Lavrik Model-based dissection of CD95 signaling dynamics reveals both a pro- and antiapoptotic role of c--FLIP$_L$ . . . 377 Brian J. Hawkins and Krishna M. Irrinki and Karthik Mallilankaraman and Yu-Chin Lien and Youjun Wang and Cunnigaiper D. Bhanumathy and Ramasamy Subbiah and Michael F. Ritchie and Jonathan Soboloff and Yoshihiro Baba and Tomohiro Kurosaki and Suresh K. Joseph and Donald L. Gill and Muniswamy Madesh S-glutathionylation activates STIM1 and alters mitochondrial homeostasis . . . . 391 Michaella Velichkova and Joe Juan and Pavan Kadandale and Steve Jean and Inês Ribeiro and Vignesh Raman and Chris Stefan and Amy A. Kiger Drosophila Mtm and class II PI3K coregulate a PI(3)P pool with cortical and endolysosomal functions . . . . . . 407 Addolorata Pisconti and D. D. W. Cornelison and Hugo C. Olguín and Tiffany L. Antwine and Bradley B. Olwin Syndecan-3 and Notch cooperate in regulating adult myogenesis . . . . . . 427 Fanny Jaulin and Geri Kreitzer KIF17 stabilizes microtubules and contributes to epithelial morphogenesis by acting at MT plus ends with EB1 and APC . . . . . . . . . . . . . . . . . . 443 Nao Hiramoto-Yamaki and Shingo Takeuchi and Shuhei Ueda and Kohei Harada and Satoshi Fujimoto and Manabu Negishi and Hironori Katoh Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism . . . . . . . . . . . . . . . 461 Wei-Ting Chao and Alexes C. Daquinag and Felicity Ashcroft and Jeannette Kunz Type I PIPK-$ \alpha $ regulates directed cell migration by modulating Rac1 plasma membrane targeting and activation . . . . . . . . . . . . . . . 479
Ben Short The cytoplasm gets some new threads . . ?? Ben Short Myosin II contracts out GTPase activation . . . . . . . . . . . . . . . ?? Ben Short Migrating cells CLIC into gear . . . . . ?? Ben Short Casting the autophagy net . . . . . . . 483 Ben Short Martin Hetzer: Taking the nuclear membrane beyond the barrier . . . . . . 484 Hai Dang Nguyen and Anja-Katrin Bielinsky HDM2 ERKs PCNA . . . . . . . . . . . . . 487 Tobias C. Walther and Matthias Mann Mass spectrometry-based proteomics in cell biology . . . . . . . . . . . . . . 491 Barbara Di Fiore and Jonathon Pines How cyclin A destruction escapes the spindle assembly checkpoint . . . . . . 501 Kohichi Matsunaga and Eiji Morita and Tatsuya Saitoh and Shizuo Akira and Nicholas T. Ktistakis and Tetsuro Izumi and Takeshi Noda and Tamotsu Yoshimori Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L . . . . . . . . . . . . . . . . . 511 Ioannis P. Nezis and Bhupendra V. Shravage and Antonia P. Sagona and Trond Lamark and Geir Bjòrkòy and Terje Johansen and Tor Erik Rusten and Andreas Brech and Eric H. Baehrecke and Harald Stenmark Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late \bionameDrosophila melanogaster oogenesis . . . . . . . . . 523 Salvatore J. Cherra and Scott M. Kulich and Guy Uechi and Manimalha Balasubramani and John Mountzouris and Billy W. Day and Charleen T. Chu Regulation of the autophagy protein LC3 by phosphorylation . . . . . . . . . . . 533 Chalongrat Noree and Brian K. Sato and Risa M. Broyer and James E. Wilhelm Identification of novel filament-forming proteins in \bionameSaccharomyces cerevisiae and \bionameDrosophila melanogaster . . . . . . . . . . . . . . 541 Sarah J. Heasman and Leo M. Carlin and Susan Cox and Tony Ng and Anne J. Ridley Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration . . . 553 Benjamin E. L. Lauffer and Cristina Melero and Paul Temkin and Cai Lei and Wanjin Hong and Tanja Kortemme and Mark von Zastrow SNX27 mediates PDZ-directed sorting from endosomes to the plasma membrane . . . . 565 Angela L. Groehler and Deborah A. Lannigan A chromatin-bound kinase, ERK8, protects genomic integrity by inhibiting HDM2-mediated degradation of the DNA clamp PCNA . . . . . . . . . . . . . . . 575 Wouter van Zon and Janneke Ogink and Bas ter Riet and René H. Medema and Hein te Riele and Rob M. F. Wolthuis The APC/C recruits cyclin B1--Cdk1--Cks in prometaphase before D box recognition to control mitotic exit . . . . . . . . 587 Tatsuya Suzuki and Hiroto Izumi and Mutsuhito Ohno Cajal body surveillance of U snRNA export complex assembly . . . . . . . . 603 Julio O. Ortiz and Florian Brandt and Valério R. F. Matias and Lau Sennels and Juri Rappsilber and Sjors H. W. Scheres and Matthias Eibauer and F. Ulrich Hartl and Wolfgang Baumeister Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ . . . . . . . . . . . 613 Vinh Q. Lam and David Akopian and Michael Rome and Doug Henningsen and Shu-ou Shan Lipid activation of the signal recognition particle receptor provides spatial coordination of protein targeting . . . . . . . . . . . . . . . 623 Ryosuke Minami and Atsuko Hayakawa and Hiroki Kagawa and Yuko Yanagi and Hideyoshi Yokosawa and Hiroyuki Kawahara BAG-6 is essential for selective elimination of defective proteasomal substrates . . . . . . . . . . . . . . . 637 Anthony Scim\`e and Vahab D. Soleimani and C. Florian Bentzinger and Mark A. Gillespie and Fabien Le Grand and Guillaume Grenier and Lisa Bevilacqua and Mary-Ellen Harper and Michael A. Rudnicki Oxidative status of muscle is determined by p107 regulation of PGC-1$ \alpha $ 651 Chan-Soo Lee and Chang-Ki Choi and Eun-Young Shin and Martin Alexander Schwartz and Eung-Gook Kim Myosin II directly binds and inhibits Dbl family guanine nucleotide exchange factors: a possible link to Rho family GTPases . . . . . . . . . . . . . . . . 663 Mark T. Howes and Matthew Kirkham and James Riches and Katia Cortese and Piers J. Walser and Fiona Simpson and Michelle M. Hill and Alun Jones and Richard Lundmark and Margaret R. Lindsay and Delia J. Hernandez-Deviez and Gordana Hadzic and Adam McCluskey and Rumasia Bashir and Libin Liu and Paul Pilch and Harvey McMahon and Phillip J. Robinson and John F. Hancock and Satyajit Mayor and Robert G. Parton Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells 675 Fei Liu and Justin D. Mih and Barry S. Shea and Alvin T. Kho and Asma S. Sharif and Andrew M. Tager and Daniel J. Tschumperlin Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression . . . . . . . . . . . . . . 693
Mitch Leslie Unwrapping new histones . . . . . . . . ?? Mitch Leslie Go on, satellite cells, be all that you can be . . . . . . . . . . . . . . . . . ?? Mitch Leslie With HMGB1's help, cells dine in . . . . ?? Mitch Leslie Sweet recipe for cellular closeness . . 709 Ben Short Mónica Bettencourt--Dias: Centered on centrioles . . . . . . . . . . . . . . . 710 Heymut Omran NPHP proteins: gatekeepers of the ciliary compartment . . . . . . . . . . 715 Peter M. Douglas and Andrew Dillin Protein homeostasis and aging in neurodegeneration . . . . . . . . . . . 719 Dorthe Helena Larsen and Catherine Poinsignon and Thorkell Gudjonsson and Christoffel Dinant and Mark R. Payne and Flurina J. Hari and Jannie M. Rendtlew Danielsen and Patrice Menard and Jette Christensen Sand and Manuel Stucki and Claudia Lukas and Jiri Bartek and Jens S. Andersen and Jiri Lukas The chromatin-remodeling factor CHD4 coordinates signaling and repair after DNA damage . . . . . . . . . . . . . . . 731 Godelieve Smeenk and Wouter W. Wiegant and Hans Vrolijk and Aldo P. Solari and Albert Pastink and Haico van Attikum The NuRD chromatin--remodeling complex regulates signaling and repair of DNA damage . . . . . . . . . . . . . . . . . 741 Michael P. Krahn and Johanna Bückers and Lars Kastrup and Andreas Wodarz Formation of a Bazooka--Stardust complex is essential for plasma membrane polarity in epithelia . . . . . . . . . 751 Paul Sinclair and Qian Bian and Matt Plutz and Edith Heard and Andrew S. Belmont Dynamic plasticity of large-scale chromatin structure revealed by self-assembly of engineered chromosome regions . . . . . . . . . . . . . . . . 761 Sonja M. Wiedemann and Silke N. Mildner and Clemens Bönisch and Lars Israel and Andreas Maiser and Sarah Matheisl and Tobias Straub and Rainer Merkl and Heinrich Leonhardt and Elisabeth Kremmer and Lothar Schermelleh and Sandra B. Hake Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y . . . . . . . . 777 Samriddha Ray and Kazunori Kume and Sneha Gupta and Wanzhong Ge and Mohan Balasubramanian and Dai Hirata and Dannel McCollum The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in \bionameSchizosaccharomyces pombe . . . 793 Guillaume Bompard and Gabriel Rabeharivelo and Marie Frank and Julien Cau and Claude Delsert and Nathalie Morin Subgroup II PAK-mediated phosphorylation regulates Ran activity during mitosis 807 Daniel P. Maskell and Xiao-Wen Hu and Martin R. Singleton Molecular architecture and assembly of the yeast kinetochore MIND complex . . . 823 Arsen Petrovic and Sebastiano Pasqualato and Prakash Dube and Veronica Krenn and Stefano Santaguida and Davide Cittaro and Silvia Monzani and Lucia Massimiliano and Jenny Keller and Aldo Tarricone and Alessio Maiolica and Holger Stark and Andrea Musacchio The MIS12 complex is a protein interaction hub for outer kinetochore assembly . . . . . . . . . . . . . . . . 835 Marie-Françoise O'Donohue and Valérie Choesmel and Marl\`ene Faubladier and Gwennaële Fichant and Pierre-Emmanuel Gleizes Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits . . . . 853 Jian-Fu Chen and Yazhong Tao and Juan Li and Zhongliang Deng and Zhen Yan and Xiao Xiao and Da-Zhi Wang microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7 . . . . . . . . . . . . 867 Daolin Tang and Rui Kang and Kristen M. Livesey and Chun-Wei Cheh and Adam Farkas and Patricia Loughran and George Hoppe and Marco E. Bianchi and Kevin J. Tracey and Herbert J. Zeh and Michael T. Lotze Endogenous HMGB1 regulates autophagy . . 881 Hung-Hsiang Huang and Pamela Stanley A testis-specific regulator of complex and hybrid N-glycan synthesis . . . . . 893 Jamil Kanaani and Julia Kolibachuk and Hugo Martinez and Steinunn Baekkeskov Two distinct mechanisms target GAD67 to vesicular pathways and presynaptic clusters . . . . . . . . . . . . . . . . 911 Branch Craige and Che-Chia Tsao and Dennis R. Diener and Yuqing Hou and Karl-Ferdinand Lechtreck and Joel L. Rosenbaum and George B. Witman CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content . . 927 Michaella Velichkova and Joe Juan and Pavan Kadandale and Steve Jean and Inês Ribeiro and Vignesh Raman and Chris Stefan and Amy A. Kiger Drosophila Mtm and class II PI3K coregulate a PI(3)P pool with cortical and endolysosomal functions . . . . . . 941
Mitch Leslie Elm1 sparks the SPOC . . . . . . . . . . ?? Ben Short Autophagy membranes held in reserve . . ?? Ben Short $ \alpha $-Synuclein leaves autophagy feeling compromised . . . . . . . . . . ?? Ben Short $ \beta $-Catenin gets an honorable discharge . . . . . . . . . . . . . . . 945 Caitlin Sedwick Noboru Mizushima: All about autophagy 946 Daniel B. Rifkin and Vesna Todorovic Bone matrix to growth factors: location, location, location . . . . . . . . . . . 949 Ching-Hwa Sung and Jen-Zen Chuang The cell biology of vision . . . . . . . 953 Roswitha Krick and Sebastian Bremer and Evelyn Welter and Petra Schlotterhose and Yvonne Muehe and Eeva-Liisa Eskelinen and Michael Thumm Cdc48\slash p97 and Shp1/p47 regulate autophagosome biogenesis in concert with ubiquitin-like Atg8 . . . . . . . . . . 965 Ayse Koca Caydasi and Bahtiyar Kurtulmus and Maria I. L. Orrico and Astrid Hofmann and Bashar Ibrahim and Gislene Pereira Elm1 kinase activates the spindle position checkpoint kinase Kin4 . . . . 975 Claudio R. Thoma and Alexandre Matov and Katrin L. Gutbrodt and Christian R. Hoerner and Zlatko Smole and Wilhelm Krek and Gaudenz Danuser Quantitative image analysis identifies pVHL as a key regulator of microtubule dynamic instability . . . . . . . . . . 991 Muriel Mari and Janice Griffith and Ester Rieter and Lakshmi Krishnappa and Daniel J. Klionsky and Fulvio Reggiori An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis . . . . . . . . 1005 Ashley R. Winslow and Chien-Wen Chen and Silvia Corrochano and Abraham Acevedo-Arozena and David E. Gordon and Andrew A. Peden and Maike Lichtenberg and Fiona M. Menzies and Brinda Ravikumar and Sara Imarisio and Steve Brown and Cahir J. O'Kane and David C. Rubinsztein $ \alpha $-Synuclein impairs macroautophagy: implications for Parkinson's disease . . . . . . . . . . 1023 Cristinel Sandu and Hyung Don Ryoo and Hermann Steller Drosophila IAP antagonists form multimeric complexes to promote cell death . . . . . . . . . . . . . . . . . 1039 Pei Ching Low and Ryo Misaki and Kate Schroder and Amanda C. Stanley and Matthew J. Sweet and Rohan D. Teasdale and Bart Vanhaesebroeck and Frédéric A. Meunier and Tomohiko Taguchi and Jennifer L. Stow Phosphoinositide $3$-kinase $ \delta $ regulates membrane fission of Golgi carriers for selective cytokine secretion . . . . . . . . . . . . . . . 1053 Yuanyuan Liu and Claudia Schirra and Ludwig Edelmann and Ulf Matti and JeongSeop Rhee and Detlef Hof and Dieter Bruns and Nils Brose and Heiko Rieger and David R. Stevens and Jens Rettig Two distinct secretory vesicle--priming steps in adrenal chromaffin cells . . . 1067 Arthit Chairoungdua and Danielle L. Smith and Pierre Pochard and Michael Hull and Michael J. Caplan Exosome release of $ \beta $-catenin: a novel mechanism that antagonizes Wnt signaling . . . . . . . . . . . . . . . 1079 Aline Bozec and Latifa Bakiri and Maria Jimenez and Thorsten Schinke and Michael Amling and Erwin F. Wagner Fra-2\slash AP-1 controls bone formation by regulating osteoblast differentiation and collagen production . . . . . . . . 1093 Harikiran Nistala and Sui Lee-Arteaga and Silvia Smaldone and Gabriella Siciliano and Luca Carta and Robert N. Ono and Gerhard Sengle and Emilio Arteaga-Solis and Regis Levasseur and Patricia Ducy and Lynn Y. Sakai and Gerard Karsenty and Francesco Ramirez Fibrillin-1 and -2 differentially modulate endogenous TGF-$ \beta $ and BMP bioavailability during bone formation . . . . . . . . . . . . . . . 1107
Mitch Leslie Ras makes a pit stop . . . . . . . . . . ?? Mitch Leslie CLIP catches enzymes in the act . . . . ?? Mitch Leslie How a virus enters without breaking . . ?? Mitch Leslie Not all nuclear pores created equal . . 3 Caitlin Sedwick Bettina Winckler: Neuronal polarity on her mind . . . . . . . . . . . . . . . . 4 Patricia Ducy and Gerard Karsenty The two faces of serotonin in bone biology . . . . . . . . . . . . . . . . 7 Elisa Dultz and Jan Ellenberg Live imaging of single nuclear pores reveals unique assembly kinetics and mechanism in interphase . . . . . . . . 15 Ryo Misaki and Miki Morimatsu and Takefumi Uemura and Satoshi Waguri and Eiji Miyoshi and Naoyuki Taniguchi and Michiyuki Matsuda and Tomohiko Taguchi Palmitoylated Ras proteins traffic through recycling endosomes to the plasma membrane during exocytosis . . . 23 Ye Xu and Yingli Sun and Xiaofeng Jiang and Marina K. Ayrapetov and Patryk Moskwa and Shenghong Yang and David M. Weinstock and Brendan D. Price The p400 ATPase regulates nucleosome stability and chromatin ubiquitination during DNA repair . . . . . . . . . . . 31 Ismail Hassan Ismail and Christi Andrin and Darin McDonald and Michael J. Hendzel BMI1-mediated histone ubiquitylation promotes DNA double-strand break repair 45 Markus Posch and Guennadi A. Khoudoli and Sam Swift and Emma M. King and Jennifer G. DeLuca and Jason R. Swedlow Sds22 regulates aurora B activity and microtubule--kinetochore interactions at mitosis . . . . . . . . . . . . . . . . 61 Martina Huranová and Ivan Ivani and Ale\vs Benda and Ina Poser and Yehuda Brody and Martin Hof and Yaron Shav-Tal and Karla M. Neugebauer and David Stan\vek The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells . . . . . . . . . . . . 75 Cristina Valacca and Serena Bonomi and Emanuele Buratti and Simona Pedrotti and Francisco Ernesto Baralle and Claudio Sette and Claudia Ghigna and Giuseppe Biamonti Sam68 regulates EMT through alternative splicing--activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene 87 Anna Raffaello and Giulia Milan and Eva Masiero and Silvia Carnio and Donghoon Lee and Gerolamo Lanfranchi and Alfred Lewis Goldberg and Marco Sandri JunB transcription factor maintains skeletal muscle mass and promotes hypertrophy . . . . . . . . . . . . . . 101 Heidi N. Fridolfsson and Daniel A. Starr Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei . . . . . . . . . . 115 Daniel Mesnard and Daniel B. Constam Imaging proprotein convertase activities and their regulation in the implanting mouse blastocyst . . . . . . . . . . . . 129 David U. Mick and Milena Vukotic and Heike Piechura and Helmut E. Meyer and Bettina Warscheid and Markus Deckers and Peter Rehling Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondria . . . . . . 141 Sabrina Di Bartolomeo and Marco Corazzari and Francesca Nazio and Serafina Oliverio and Gaia Lisi and Manuela Antonioli and Vittoria Pagliarini and Silvia Matteoni and Claudia Fuoco and Luigi Giunta and Marcello D'Amelio and Roberta Nardacci and Alessandra Romagnoli and Mauro Piacentini and Francesco Cecconi and Gian Maria Fimia The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy . . . . . . . . . . 155 Rebecca W. Scott and Steven Hooper and Diane Crighton and Ang Li and Ireen König and June Munro and Elisabeth Trivier and Grant Wickman and Pierre Morin and Daniel R. Croft and John Dawson and Laura Machesky and Kurt I. Anderson and Erik A. Sahai and Michael F. Olson LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells . . . . . 169 Colin P. Johnson and Edwin R. Chapman Otoferlin is a calcium sensor that directly regulates SNARE-mediated membrane fusion . . . . . . . . . . . . 187 Sonia Libersou and Aurélie A. V. Albertini and Malika Ouldali and Virginie Maury and Christine Maheu and Hél\`ene Raux and Felix de Haas and Stéphane Roche and Yves Gaudin and Jean Lepault Distinct structural rearrangements of the VSV glycoprotein drive membrane fusion . . . . . . . . . . . . . . . . . 199 Xin Xiong and Xin Wang and Ronny Ewanek and Pavan Bhat and Aaron DiAntonio and Catherine A. Collins Protein turnover of the Wallenda/DLK kinase regulates a retrograde response to axonal injury . . . . . . . . . . . . 211
Ben Short Augmin-ting the central spindle . . . . ?? Ben Short Rab GEFs emerge from their DENN . . . . ?? Ben Short Gli inhibitor pricked by Hedgehog . . . ?? Ben Short A little Cdc20 goes a long way . . . . . 227 Caitlin Sedwick Jiahuai Han: Aflame on inflammation and p38 . . . . . . . . . . . . . . . . . . 228 Aimee deCathelineau and Elizabeth H. Williams and Tom Misteli and Mike Rossner Friends, colleagues, authors, lend us your data . . . . . . . . . . . . . . . 231 Jagesh V. Shah Cells in tight spaces: the role of cell shape in cell function . . . . . . . . . 233 Ellen A. Lumpkin and Kara L. Marshall and Aislyn M. Nelson The cell biology of touch . . . . . . . 237 Liyi Geng and Catherine J. Huntoon and Larry M. Karnitz RAD18-mediated ubiquitination of PCNA activates the Fanconi anemia DNA repair network . . . . . . . . . . . . . . . . 249 Ryota Uehara and Gohta Goshima Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase . . . . . . . . . . . . . . . . 259 Jens C. Schmidt and Tomomi Kiyomitsu and Tetsuya Hori and Chelsea B. Backer and Tatsuo Fukagawa and Iain M. Cheeseman Aurora B kinase controls the targeting of the Astrin--SKAP complex to bioriented kinetochores . . . . . . . . 269 Nannette Jelluma and Tobias B. Dansen and Tale Sliedrecht and Nicholas P. Kwiatkowski and Geert J. P. L. Kops Release of Mps1 from kinetochores is crucial for timely anaphase onset . . . 281 Giovanni Ciavarra and Eldad Zacksenhaus Rescue of myogenic defects in Rb-deficient cells by inhibition of autophagy or by hypoxia-induced glycolytic shift . . . . . . . . . . . . 291 Amandine Pitaval and Qingzong Tseng and Michel Bornens and Manuel Théry Cell shape and contractility regulate ciliogenesis in cell cycle--arrested cells . . . . . . . . . . . . . . . . . 303 Liviu Malureanu and Karthik B. Jeganathan and Fang Jin and Darren J. Baker and Janine H. van Ree and Oliver Gullon and Zheyan Chen and John R. Henley and Jan M. van Deursen Cdc20 hypomorphic mice fail to counteract de novo synthesis of cyclin B1 in mitosis . . . . . . . . . . . . . 313 Moe R. Mahjoub and Zhigang Xie and Tim Stearns Cep120 is asymmetrically localized to the daughter centriole and is essential for centriole assembly . . . . . . . . . 331 Hiroyuki Hirai and Mayank Verma and Shuichi Watanabe and Christopher Tastad and Yoko Asakura and Atsushi Asakura MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3 . . . . . . . . 347 Shin-ichiro Yoshimura and Andreas Gerondopoulos and Andrea Linford and Daniel J. Rigden and Francis A. Barr Family-wide characterization of the DENN domain Rab GDP--GTP exchange factors . . 367 Anna Brachet and Christophe Leterrier and Marie Irondelle and Marie-Pierre Fache and Victor Racine and Jean-Baptiste Sibarita and Daniel Choquet and Bénédicte Dargent Ankyrin G restricts ion channel diffusion at the axonal initial segment before the establishment of the diffusion barrier . . . . . . . . . . . 383 Damien Garbett and David P. LaLonde and Anthony Bretscher The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner . . . . 397 Hanna Tukachinsky and Lyle V. Lopez and Adrian Salic A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu--Gli protein complexes . . . . . . . . . . . . . . . 415 Nirupa Chaudhari and Stephen D. Roper The cell biology of taste . . . . . . . 429 Mitch Leslie CLIP catches enzymes in the act . . . . 431
Mitch Leslie A MAP holds the corners . . . . . . . . ?? Mitch Leslie dRich doesn't take sides . . . . . . . . ?? Mitch Leslie Cytoplasmic enzymes cast NETs . . . . . ?? Mitch Leslie Helping stem cells make a connection . . 435 Ben Short Maxence Nachury: a transporting view of the primary cilium . . . . . . . . . . . 436 Robert G. Parton and Mark T. Howes Revisiting caveolin trafficking: the end of the caveosome . . . . . . . . . . . . 439 Shannon DeMaria and John Ngai The cell biology of smell . . . . . . . 443 Masaru Mitsushima and Kazuhiro Aoki and Miki Ebisuya and Shigeru Matsumura and Takuya Yamamoto and Michiyuki Matsuda and Fumiko Toyoshima and Eisuke Nishida Revolving movement of a dynamic cluster of actin filaments during mitosis . . . 453 Franck J. Fourniol and Charles V. Sindelar and Béatrice Amigues and Daniel K. Clare and Geraint Thomas and Myl\`ene Perderiset and Fiona Francis and Anne Houdusse and Carolyn A. Moores Template-free $ 13$-protofilament microtubule--MAP assembly visualized at 8 Å resolution . . . . . . . . . . . . . 463 Michael V. D'Ambrosio and Ronald D. Vale A whole genome RNAi screen of \bionameDrosophila S2 cell spreading performed using automated computational image analysis . . . . . . . . . . . . . 471 Rebecca M. Fox and Caitlin D. Hanlon and Deborah J. Andrew The CrebA/Creb3-like transcription factors are major and direct regulators of secretory capacity . . . . . . . . . 479 Jeffrey K. Moore and Prakash Chudalayandi and Richard A. Heil-Chapdelaine and John A. Cooper The spindle position checkpoint is coordinated by the Elm1 kinase . . . . . 493 Jana M. Mitchell and Jörg Mansfeld and Juliana Capitanio and Ulrike Kutay and Richard W. Wozniak Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane . . . . . . . . . . . . . . . . 505 Fang Yu and Lu Sun and Khaled Machaca Constitutive recycling of the store-operated Ca$^{2+}$ channel Orai1 and its internalization during meiosis 523 Brigit E. Riley and Stephen E. Kaiser and Thomas A. Shaler and Aylwin C. Y. Ng and Taichi Hara and Mark S. Hipp and Kasper Lage and Ramnik J. Xavier and Kwon-Yul Ryu and Keiko Taguchi and Masayuki Yamamoto and Keiji Tanaka and Noboru Mizushima and Masaaki Komatsu and Ron R. Kopito Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection . . . . . . . . . . . . . . . 537 Pirjo M. Apaja and Haijin Xu and Gergely L. Lukacs Quality control for unfolded proteins at the plasma membrane . . . . . . . . . . 553 Scott D. Hansen and R. Dyche Mullins VASP is a processive actin polymerase that requires monomeric actin for barbed end association . . . . . . . . . . . . 571 Jean-Baptiste Manneville and Muguette Jehanno and Sandrine Etienne-Manneville Dlg1 binds GKAP to control dynein association with microtubules, centrosome positioning, and cell polarity . . . . . . . . . . . . . . . . 585 Andrew R. Flannery and Cecilia Czibener and Norma W. Andrews Palmitoylation-dependent association with CD63 targets the Ca$^{2+}$ sensor synaptotagmin VII to lysosomes . . . . . 599 Arnold Hayer and Miriam Stoeber and Danilo Ritz and Sabrina Engel and Hemmo H. Meyer and Ari Helenius Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in endolysosomes for degradation . . . . . 615 Dong Li and Jiaxi Zhou and Lu Wang and Myung Eun Shin and Pei Su and Xiaohua Lei and Haibin Kuang and Weixiang Guo and Hong Yang and Linzhao Cheng and Tetsuya S. Tanaka and Deborah E. Leckband and Albert B. Reynolds and Enkui Duan and Fei Wang Integrated biochemical and mechanical signals regulate multifaceted human embryonic stem cell functions . . . . . 631 Nadim Maghzal and Emily Vogt and Wolfgang Reintsch and James S. Fraser and François Fagotto The tumor-associated EpCAM regulates morphogenetic movements through intracellular signaling . . . . . . . . 645 Minyeop Nahm and A. Ashleigh Long and Sang Kyoo Paik and Sungdae Kim and Yong Chul Bae and Kendal Broadie and Seungbok Lee The Cdc42-selective GAP Rich regulates postsynaptic development and retrograde BMP transsynaptic signaling . . . . . . 661 Venizelos Papayannopoulos and Kathleen D. Metzler and Abdul Hakkim and Arturo Zychlinsky Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps . . . . . . . . . . 677
Ben Short SRGP-1 drives cell junctions round the bend . . . . . . . . . . . . . . . . . . ?? Ben Short Cells find an alternative exit . . . . . ?? Ben Short How Vps41 HOPS between tethering functions . . . . . . . . . . . . . . . ?? Ben Short Cep152 and Plk4 form a double act . . . 695 Caitlin Sedwick Craig Thompson: The method to cancer's madness . . . . . . . . . . . . . . . . 696 Hansjörg Schwertz and Andrew S. Weyrich Platelet precursors display bipolar behavior . . . . . . . . . . . . . . . . 699 Hannah C. Chapin and Michael J. Caplan The cell biology of polycystic kidney disease . . . . . . . . . . . . . . . . 701 Yan Hu and Matt Plutz and Andrew S. Belmont Hsp70 gene association with nuclear speckles is Hsp70 promoter specific . . 711 Emily M. Hatch and Anita Kulukian and Andrew J. Holland and Don W. Cleveland and Tim Stearns Cep152 interacts with Plk4 and is required for centriole duplication . . . 721 Onur Cizmecioglu and Marc Arnold and Ramona Bahtz and Florian Settele and Lena Ehret and Uta Haselmann-Weiß and Claude Antony and Ingrid Hoffmann Cep152 acts as a scaffold for recruitment of Plk4 and CPAP to the centrosome . . . . . . . . . . . . . . . 731 Mathew P. Estey and Caterina Di Ciano-Oliveira and Carol D. Froese and Margaret T. Bejide and William S. Trimble Distinct roles of septins in cytokinesis: SEPT9 mediates midbody abscission . . . . . . . . . . . . . . . 741 Ricardo Nunes Bastos and Francis A. Barr Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission . . . . . . . . . . . 751 Ronen Zaidel-Bar and Michael J. Joyce and Allison M. Lynch and Kristen Witte and Anjon Audhya and Jeff Hardin The F--BAR domain of SRGP-1 facilitates cell--cell adhesion during \bionameC. elegans morphogenesis . . . . . . . . . 761 Alexander Ludwig and Grant P. Otto and Kirsi Riento and Emily Hams and Padraic G. Fallon and Ben J. Nichols Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment . . 771 Hyunju Ryu and Maiko Furuta and Donald Kirkpatrick and Steven P. Gygi and Yoshiaki Azuma PIASy-dependent SUMOylation regulates DNA topoisomerase II$ \alpha $ activity 783 Jeffrey B. Woodruff and David G. Drubin and Georjana Barnes Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8 . . . . . . . . . 795 Stacey E. Wirt and Adam S. Adler and Véronique Gebala and James M. Weimann and Bethany E. Schaffer and Louis A. Saddic and Patrick Viatour and Hannes Vogel and Howard Y. Chang and Alex Meissner and Julien Sage G1 arrest and differentiation can occur independently of Rb family function . . 809 Zhixun Dou and Mohar Chattopadhyay and Ji-An Pan and Jennifer L. Guerriero and Ya-Ping Jiang and Lisa M. Ballou and Zhenyu Yue and Richard Z. Lin and Wei-Xing Zong The class IA phosphatidylinositol $3$-kinase p110-$ \beta $ subunit is a positive regulator of autophagy . . . . 827 Margarita Cabrera and Lars Langemeyer and Muriel Mari and Ralf Rethmeier and Ioan Orban and Angela Perz and Cornelia Bröcker and Janice Griffith and Daniel Klose and Heinz-Jürgen Steinhoff and Fulvio Reggiori and Siegfried Engelbrecht-Vandré and Christian Ungermann Phosphorylation of a membrane curvature--sensing motif switches function of the HOPS subunit Vps41 in membrane tethering . . . . . . . . . . . 845 Jonathan N. Thon and Alejandro Montalvo and Sunita Patel-Hett and Matthew T. Devine and Jennifer L. Richardson and Allen Ehrlicher and Mark K. Larson and Karin Hoffmeister and John H. Hartwig and Joseph E. Italiano Cytoskeletal mechanics of proplatelet maturation and platelet release . . . . 861 Martin Distel and Jennifer C. Hocking and Katrin Volkmann and Reinhard W. Köster The centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in vivo . . . . . . . . . . . . . . . . . . 875 Quint le Duc and Quanming Shi and Iris Blonk and Arnoud Sonnenberg and Ning Wang and Deborah Leckband and Johan de Rooij Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner . . . . . . . . . . . . . . . . . 891
Ben Short Boi keeps Hedgehog close to home . . . . ?? Ben Short Rb gets ERK'd by the competition . . . . ?? Ben Short PIPs propel phagosomes . . . . . . . . . ?? Ben Short Setting the spindle's compass . . . . . 895 Caitlin Sedwick Ted Salmon: Kinetochores at the core of it all . . . . . . . . . . . . . . . . . 896 David M. Gilbert Cell fate transitions and the replication timing decision point . . . 899 Jaspreet S. Khurana and William Theurkauf piRNAs, transposon silencing, and \bionameDrosophila germline development 905 Nicholas D. Poulson and Terry Lechler Robust control of mitotic spindle orientation in the developing epidermis 915 Douglas R. Mackay and Masaki Makise and Katharine S. Ullman Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint . . . . . . . . . 923 Seok Min Jin and Michael Lazarou and Chunxin Wang and Lesley A. Kane and Derek P. Narendra and Richard J. Youle Mitochondrial membrane potential regulates PINK1 import and proteolytic destabilization by PARL . . . . . . . . 933 Tiffiney R. Hartman and Daniel Zinshteyn and Heather K. Schofield and Emmanuelle Nicolas and Ami Okada and Alana M. O'Reilly Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation . . . . . . . . . . . . . 943 Tovah A. Day and Komariah Palle and Laura R. Barkley and Naoko Kakusho and Ying Zou and Satoshi Tateishi and Alain Verreault and Hisao Masai and Cyrus Vaziri Phosphorylated Rad18 directs DNA Polymerase $ \eta $ to sites of stalled replication . . . . . . . . . . . . . . 953 Javier Rodríguez and Fernando Calvo and José M. González and Berta Casar and Vicente Andrés and Piero Crespo ERK1\slash 2 MAP kinases promote cell cycle entry by rapid, kinase-independent disruption of retinoblastoma--lamin A complexes . . . . . . . . . . . . . . . 967 Valentina Rossio and Elena Galati and Matteo Ferrari and Achille Pellicioli and Takashi Sutani and Katsuhiko Shirahige and Giovanna Lucchini and Simonetta Piatti The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase . . . 981 Michal Bohdanowicz and Gabriela Cosío and Jonathan M. Backer and Sergio Grinstein Class I and class III phosphoinositide $3$-kinases are required for actin polymerization that propels phagosomes 999 Kristin L. Sens and Shiliang Zhang and Peng Jin and Rui Duan and Guofeng Zhang and Fengbao Luo and Lauren Parachini and Elizabeth H. Chen An invasive podosome-like structure promotes fusion pore formation during myoblast fusion . . . . . . . . . . . . 1013 Sayantanee Biswas and Michelle R. Emond and James D. Jontes Protocadherin-19 and N-cadherin interact to control cell movements during anterior neurulation . . . . . . . . . . 1029
Mitch Leslie Ino80 tidies up after DNA repair . . . . ?? Mitch Leslie Mad2 shifts a motor into idle . . . . . ?? Mitch Leslie Doubling up on cellular stress . . . . . ?? Mitch Leslie Mitochondria help dynamins get a grip 1045 Caitlin Sedwick Liz Rhoades: Single molecules tell many stories . . . . . . . . . . . . . . . . 1046 Daphne Selvaggia Cabianca and Davide Gabellini FSHD: copy number variations on the theme of muscular dystrophy . . . . . . 1049 Sovan Sarkar and Rhian Kiely and Peter J. McHugh The Ino80 chromatin-remodeling complex restores chromatin structure during UV DNA damage repair . . . . . . . . . . . 1061 Sang Hyun Lee and Frank McCormick and Hideyuki Saya Mad2 inhibits the mitotic kinesin MKlp2 1069 Micah T. Webster and J. Michael McCaffery and Orna Cohen-Fix Vesicle trafficking maintains nuclear shape in \bionameSaccharomyces cerevisiae during membrane proliferation 1079 Yuk-Kwan Choi and Pengfei Liu and Siu Kwan Sze and Chao Dai and Robert Z. Qi CDK5RAP2 stimulates microtubule nucleation by the $ \gamma $-tubulin ring complex . . . . . . . . . . . . . . 1089 Marco Geymonat and Adonis Spanos and Sanne Jensen and Steven G. Sedgwick Phosphorylation of Lte1 by Cdk prevents polarized growth during mitotic arrest in S. cerevisiae . . . . . . . . . . . . 1097 Gang Li and Christopher Scull and Lale Ozcan and Ira Tabas NADPH oxidase links endoplasmic reticulum stress, oxidative stress, and PKR activation to induce apoptosis . . . 1113 Sajjan Koirala and Huyen T. Bui and Heidi L. Schubert and Debra M. Eckert and Christopher P. Hill and Michael S. Kay and Janet M. Shaw Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexes . . . . 1127 Hidenori Otera and Chunxin Wang and Megan M. Cleland and Kiyoko Setoguchi and Sadaki Yokota and Richard J. Youle and Katsuyoshi Mihara Mff is an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells 1141 Thomas Iskratsch and Stephan Lange and Joseph Dwyer and Ay Lin Kho and Cris dos Remedios and Elisabeth Ehler Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance . . . . . 1159 Cédric S. Asensio and Daniel W. Sirkis and Robert H. Edwards RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway . . . . . . 1173 Eva Nievergall and Peter W. Janes and Carolin Stegmayer and Mary E. Vail and Fawaz G. Haj and Shyh Wei Teng and Benjamin G. Neel and Philippe I. Bastiaens and Martin Lackmann PTP1B regulates Eph receptor function and trafficking . . . . . . . . . . . . 1189 Ronald S. Flannagan and Rene E. Harrison and Christopher M. Yip and Khuloud Jaqaman and Sergio Grinstein Dynamic macrophage ``probing'' is required for the efficient capture of phagocytic targets . . . . . . . . . . . 1205
Ben Short Zfp521 works its zinc fingers to the bone . . . . . . . . . . . . . . . . . . ?? Ben Short Telomeres get SIRT-ified . . . . . . . . ?? Ben Short Myo1 provides a ring in the tail . . . . ?? Ben Short PP6 puts the brakes on spindle assembly 1221 Caitlin Sedwick Ramanujan Hegde: The prion puzzle and protein translocation . . . . . . . . . 1222 Leo J. Pallanck Culling sick mitochondria from the herd 1225 Christine Salaun and Jennifer Greaves and Luke H. Chamberlain The intracellular dynamic of protein palmitoylation . . . . . . . . . . . . . 1229 Rose Loughlin and Rebecca Heald and François Nédélec A computational model predicts \bionameXenopus meiotic spindle organization . . . . . . . . . . . . . . 1239 Manuel Breuer and Agnieszka Kolano and Mijung Kwon and Chao-Chin Li and Ting-Fen Tsai and David Pellman and Stéphane Brunet and Marie-Hél\`ene Verlhac HURP permits MTOC sorting for robust meiotic spindle bipolarity, similar to extra centrosome clustering in cancer cells . . . . . . . . . . . . . . . . . 1251 Naël Osmani and Florent Peglion and Philippe Chavrier and Sandrine Etienne-Manneville Cdc42 localization and cell polarity depend on membrane traffic . . . . . . . 1261 Eric Hesse and Hiroaki Saito and Riku Kiviranta and Diego Correa and Kei Yamana and Lynn Neff and Daniel Toben and Georg Duda and Azeddine Atfi and Valérie Geoffroy and William C. Horne and Roland Baron Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity . . . . . . . . . . . . . . . . 1271 Xin Quan Ge and J. Julian Blow Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories . . . . . . 1285 Jose A. Palacios and Daniel Herranz and Maria Luigia De Bonis and Susana Velasco and Manuel Serrano and Maria A. Blasco SIRT1 contributes to telomere maintenance and augments global homologous recombination . . . . . . . . 1299 Kang Zeng and Ricardo Nunes Bastos and Francis A. Barr and Ulrike Gruneberg Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2 . . . . . . 1315 Xiaodong Fang and Jianying Luo and Ryuichi Nishihama and Carsten Wloka and Christopher Dravis and Mirko Travaglia and Masayuki Iwase and Elizabeth A. Vallen and Erfei Bi Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II . . . . . . . . . . . . . . . 1333 Emilie Montembault and Wei Zhang and Marcin R. Przewloka and Vincent Archambault and Emeric W. Sevin and Ernest D. Laue and David M. Glover and Pier Paolo D'Avino Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in \bionameDrosophila spermatocytes . . . . 1351 Atsushi Tanaka and Megan M. Cleland and Shan Xu and Derek P. Narendra and Der-Fen Suen and Mariusz Karbowski and Richard J. Youle Proteasome and p97 mediate mitophagy and degradation of mitofusins induced by Parkin . . . . . . . . . . . . . . . . . 1367 Allen P. Liu and François Aguet and Gaudenz Danuser and Sandra L. Schmid Local clustering of transferrin receptors promotes clathrin-coated pit initiation . . . . . . . . . . . . . . . 1381 Pradyut K. Paul and Sanjay K. Gupta and Shephali Bhatnagar and Siva K. Panguluri and Bryant G. Darnay and Yongwon Choi and Ashok Kumar Targeted ablation of TRAF6 inhibits skeletal muscle wasting in mice . . . . 1395 Martin Distel and Jennifer C. Hocking and Katrin Volkmann and Reinhard W. Köster The centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in vivo . . . . . . . . . . . . . . . . . . 1413
Mitch Leslie Syndecan-3 gets the message . . . . . . ?? Mitch Leslie Cancer cells mothball mRNA after microtubule collapse . . . . . . . . . . ?? Mitch Leslie Receptors flex to remove neurotransmitter brake . . . . . . . . . ?? Mitch Leslie Two microscopes are better than one . . 3 Ben Short Sean Munro: Revealing the Golgi's true identity . . . . . . . . . . . . . . . . 4 Christof Osman and Dennis R. Voelker and Thomas Langer Making heads or tails of phospholipids in mitochondria . . . . . . . . . . . . 7 Eisuke Itakura and Noboru Mizushima p62 targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding 17 Marjorie A. Kuipers and Timothy J. Stasevich and Takayo Sasaki and Korey A. Wilson and Kristin L. Hazelwood and James G. McNally and Michael W. Davidson and David M. Gilbert Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload . . . . . . . . . 29 Stine Jòrgensen and Morten Eskildsen and Kasper Fugger and Lisbeth Hansen and Marie Sofie Yoo Larsen and Arne Nedergaard Kousholt and Randi G. Syljuåsen and Morten Beck Trelle and Ole Nòrregaard Jensen and Kristian Helin and Claus Storgaard Sòrensen SET8 is degraded via PCNA-coupled CRL4(CDT2) ubiquitylation in S phase and after UV irradiation . . . . . . . . . . 43 Sean M. Hartig and Bin He and Weiwen Long and Benjamin M. Buehrer and Michael A. Mancini Homeostatic levels of SRC-2 and SRC-3 promote early human adipogenesis . . . . 55 Yejing Ge and Yuting Sun and Jie Chen IGF-II is regulated by microRNA-125b in skeletal myogenesis . . . . . . . . . . 69 Marisa Carbonaro and Aurora O'Brate and Paraskevi Giannakakou Microtubule disruption targets HIF-1$ \alpha $ mRNA to cytoplasmic P-bodies for translational repression . . . . . . 83 Zhimin Wang and Ceba Humphrey and Nicole Frilot and Gaofeng Wang and Zhongzhen Nie and Nader H. Moniri and Yehia Daaka Dynamin2- and endothelial nitric oxide synthase--regulated invasion of bladder epithelial cells by uropathogenic \bionameEscherichia coli . . . . . . . . 101 Wanda Kukulski and Martin Schorb and Sonja Welsch and Andrea Picco and Marko Kaksonen and John A. G. Briggs Correlated fluorescence and $3$D electron microscopy with high sensitivity and spatial precision . . . 111 Ximing Du and Jaspal Kumar and Charles Ferguson and Timothy A. Schulz and Yan Shan Ong and Wanjin Hong and William A. Prinz and Robert G. Parton and Andrew J. Brown and Hongyuan Yang A role for oxysterol-binding protein--related protein 5 in endosomal cholesterol trafficking . . . . . . . . 121 Yonatan M. Kupchik and Ofra Barchad-Avitzur and Jürgen Wess and Yair Ben-Chaim and Itzchak Parnas and Hanna Parnas A novel fast mechanism for GPCR-mediated signal transduction --- control of neurotransmitter release . . . . . . . . 137 Maxim M. Bespalov and Yulia A. Sidorova and Sarka Tumova and Anni Ahonen-Bishopp and Ana Cathia Magalhães and Evgeny Kulesskiy and Mikhail Paveliev and Claudio Rivera and Heikki Rauvala and Mart Saarma Heparan sulfate proteoglycan syndecan-3 is a novel receptor for GDNF, neurturin, and artemin . . . . . . . . . . . . . . 153 Vinicio A. de Jesus Perez and Ziad Ali and Tero-Pekka Alastalo and Fumiaki Ikeno and Hirofumi Sawada and Ying-Ju Lai and Thomas Kleisli and Edda Spiekerkoetter and Xiumei Qu and Laura H. Rubinos and Euan Ashley and Manuel Amieva and Shoukat Dedhar and Marlene Rabinovitch BMP promotes motility and represses growth of smooth muscle cells by activation of tandem Wnt pathways . . . 171 Alexandre Djiane and Hideyuki Shimizu and Marian Wilkin and Sabine Mazleyrat and Martin D. Jennings and Johanna Avis and Sarah Bray and Martin Baron Su(dx) E3 ubiquitin ligase-dependent and --independent functions of Polychaetoid, the \bionameDrosophila ZO-1 homologue 189 Javier Rodríguez and Fernando Calvo and José M. González and Berta Casar and Vicente Andrés and Piero Crespo ERK1\slash 2 MAP kinases promote cell cycle entry by rapid, kinase-independent disruption of retinoblastoma--lamin A complexes . . . . . . . . . . . . . . . 201
Mitch Leslie ESCRT-III has an escort . . . . . . . . ?? Mitch Leslie Stiff ECM puts the brakes on microtubule growth . . . . . . . . . . . . . . . . . ?? Mitch Leslie N-WASP allows axons to cover up . . . . ?? Mitch Leslie Helping the embryo find closure . . . . 205 Ben Short Matthew Welch: The many branches of actin regulation . . . . . . . . . . . . 206 Patricia A. J. Muller and Karen H. Vousden and Jim C. Norman p53 and its mutants in tumor cell migration and invasion . . . . . . . . . 209 Thomas Göhler and Simone Sabbioneda and Catherine M. Green and Alan R. Lehmann ATR-mediated phosphorylation of DNA polymerase $ \eta $ is needed for efficient recovery from UV damage . . . 219 Daniel K. Stringer and Robert C. Piper A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination . . . . 229 Nurit Novak and Vered Bar and Helena Sabanay and Shahar Frechter and Martine Jaegle and Scott B. Snapper and Dies Meijer and Elior Peles N-WASP is required for membrane wrapping and myelination by Schwann cells . . . . 243 Hong Yan and Thomas Toczylowski and Jill McCane and Chinyi Chen and Shuren Liao Replication protein A promotes $ 5 ' \to 3 ' $ end processing during homology-dependent DNA double-strand break repair . . . . . . . . . . . . . . 251 Jibak Lee and Tatsuya Hirano RAD21L, a novel cohesin subunit implicated in linking homologous chromosomes in mammalian meiosis . . . . 263 Benjamin Albert and Isabelle Léger-Silvestre and Christophe Normand and Martin K. Ostermaier and Jorge Pérez-Fernández and Kostya I. Panov and Joost C. B. M. Zomerdijk and Patrick Schultz and Olivier Gadal RNA polymerase I--specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle . . . 277 Megan Wemmer and Ishara Azmi and Matthew West and Brian Davies and David Katzmann and Greg Odorizzi Bro1 binding to Snf7 regulates ESCRT--III membrane scission activity in yeast . . . . . . . . . . . . . . . . . 295 Meital Hatan and Vera Shinder and David Israeli and Frank Schnorrer and Talila Volk The \bionameDrosophila blood brain barrier is maintained by GPCR-dependent dynamic actin structures . . . . . . . . 307 Kenneth A. Myers and Kathryn T. Applegate and Gaudenz Danuser and Robert S. Fischer and Clare M. Waterman Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesis 321 Caroline Laplante and Laura A. Nilson Asymmetric distribution of Echinoid defines the epidermal leading edge during \bionameDrosophila dorsal closure 335 Shan Zhou and Lihsia Chen Neural integrity is maintained by dystrophin in \bionameC. elegans . . . . 349 Peisong Ma and Shannon L. Beck and Ronald W. Raab and Robert L. McKown and George L. Coffman and Atsushi Utani and William J. Chirico and Alan C. Rapraeger and Gordon W. Laurie Heparanase deglycanation of syndecan-1 is required for binding of the epithelial-restricted prosecretory mitogen lacritin . . . . . . . . . . . . 365
Ben Short Mutant phagosomes bite off more than they can chew . . . . . . . . . . . . . ?? Ben Short Sir3 helps telomeres stick together . . ?? Ben Short IRSp53 keeps ECM signaling up to Par . . ?? Ben Short BRCA1 on the move . . . . . . . . . . . 369 Caitlin Sedwick María Blasco: Keeping a cap on cancer and aging . . . . . . . . . . . . . . . . . 370 Min Yu and Shannon Stott and Mehmet Toner and Shyamala Maheswaran and Daniel A. Haber Circulating tumor cells: approaches to isolation and characterization . . . . . 373 Mohammed Ali Akbar and Charles Tracy and Walter H. A. Kahr and Helmut Krämer The full-of-bacteria gene is required for phagosome maturation during immune defense in \bionameDrosophila . . . . . 383 Pauline G. Knox and Clare C. Davies and Marina Ioannou and Aristides G. Eliopoulos The death domain kinase RIP1 links the immunoregulatory CD40 receptor to apoptotic signaling in carcinomas . . . 391 René M. Overmeer and Jill Moser and Marcel Volker and Hanneke Kool and Alan E. Tomkinson and Albert A. van Zeeland and Leon H. F. Mullenders and Maria Fousteri Replication protein A safeguards genome integrity by controlling NER incision events . . . . . . . . . . . . . . . . . 401 Myriam Ruault and Arnaud De Meyer and Isabelle Lo\"\iodice and Angela Taddei Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast . . 417 Eliza \.Zy\lkiewicz and Monika Kija\'nska and Won-Chan Choi and Urszula Derewenda and Zygmunt S. Derewenda and P. Todd Stukenberg The N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein . . . . . . . . . . . . . . . . . 433 Steven M. Claypool and Kevin Whited and Santi Srijumnong and Xianlin Han and Carla M. Koehler Barth syndrome mutations that cause tafazzin complex lability . . . . . . . 447 Rinshi S. Kasai and Kenichi G. N. Suzuki and Eric R. Prossnitz and Ikuko Koyama-Honda and Chieko Nakada and Takahiro K. Fujiwara and Akihiro Kusumi Full characterization of GPCR monomer--dimer dynamic equilibrium by single molecule imaging . . . . . . . . 463 Anindya Sen and Takakazu Yokokura and Mark W. Kankel and Douglas N. Dimlich and Jan Manent and Subhabrata Sanyal and Spyros Artavanis-Tsakonas Modeling spinal muscular atrophy in \bionameDrosophila links Smn to FGF signaling . . . . . . . . . . . . . . . 481 Elisabeth D. Coene and Catarina Gadelha and Nicholas White and Ashraf Malhas and Benjamin Thomas and Michael Shaw and David J. Vaux A novel role for BRCA1 in regulating breast cancer cell spreading and motility . . . . . . . . . . . . . . . . 497 Minna Poukkula and Adam Cliffe and Rishita Changede and Pernille Ròrth Cell behaviors regulated by guidance cues in collective migration of border cells . . . . . . . . . . . . . . . . . 513 David Cohen and Dawn Fernandez and Francisco Lázaro-Diéguez and Anne Müsch The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell--ECM signaling . . 525
Mitch Leslie Sphingolipid imbalance can take out an eye . . . . . . . . . . . . . . . . . . ?? Mitch Leslie Annexin keeps tau on a short leash . . . ?? Mitch Leslie Natural killer cells commute death sentence . . . . . . . . . . . . . . . . ?? Mitch Leslie Hedgehog, meet Patched . . . . . . . . . 543 Caitlin Sedwick Harald Stenmark: Hands on FYVE-fingers 544 Francis Rodier and Judith Campisi Four faces of cellular senescence . . . 547 Ikuko Yonamine and Takeshi Bamba and Niraj K. Nirala and Nahid Jesmin and Teresa Kosakowska-Cholody and Kunio Nagashima and Eiichiro Fukusaki and Jairaj K. Acharya and Usha Acharya Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors . . . . . 557 Rafael Bernad and Patricia Sánchez and Teresa Rivera and Miriam Rodríguez-Corsino and Ekaterina Boyarchuk and Isabelle Vassias and Dominique Ray-Gallet and Alexei Arnaoutov and Mary Dasso and Genevi\`eve Almouzni and Ana Losada Xenopus HJURP and condensin II are required for CENP--A assembly . . . . . 569 Cornelia Kurischko and Hong Kyung Kim and Venkata K. Kuravi and Juliane Pratzka and Francis C. Luca The yeast Cbk1 kinase regulates mRNA localization via the mRNA-binding protein Ssd1 . . . . . . . . . . . . . . 583 Mauricio Valerio-Santiago and Fernando Monje-Casas Tem1 localization to the spindle pole bodies is essential for mitotic exit and impairs spindle checkpoint function . . 599 Eugenia Morselli and Guillermo Mariño and Martin V. Bennetzen and Tobias Eisenberg and Evgenia Megalou and Sabrina Schroeder and Sandra Cabrera and Paule Bénit and Pierre Rustin and Alfredo Criollo and Oliver Kepp and Lorenzo Galluzzi and Shensi Shen and Shoaib Ahmad Malik and Maria Chiara Maiuri and Yoshiyuki Horio and Carlos López-Otín and Jens S. Andersen and Nektarios Tavernarakis and Frank Madeo and Guido Kroemer Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome . . . . 615 Helen Hoffmeister and Karin Babinger and Sonja Gürster and Anna Cedzich and Christine Meese and Karin Schadendorf and Larissa Osten and Uwe de Vries and Anne Rascle and Ralph Witzgall Polycystin-2 takes different routes to the somatic and ciliary plasma membrane 631 Anne Gauthier-Kemper and Carina Weissmann and Nataliya Golovyashkina and Zsofia Sebö-Lemke and Gerard Drewes and Volker Gerke and Jürgen J. Heinisch and Roland Brandt The frontotemporal dementia mutation R406W blocks tau's interaction with the membrane in an annexin A2-dependent manner . . . . . . . . . . . . . . . . . 647 Andy Tsun and Ihjaaz Qureshi and Jane C. Stinchcombe and Misty R. Jenkins and Maike de la Roche and Joanna Kleczkowska and Rose Zamoyska and Gillian M. Griffiths Centrosome docking at the immunological synapse is controlled by Lck signaling 663 Thushara P. Abeyweera and Ernesto Merino and Morgan Huse Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells . . . . . . . . . . . . . . 675 Fuchuan Li and Wen Shi and Mariana Capurro and Jorge Filmus Glypican-5 stimulates rhabdomyosarcoma cell proliferation by activating Hedgehog signaling . . . . . . . . . . . 691
Ben Short Rad54 mutant stays rooted to the spot ?? Ben Short A fifth amendment to the intestine's constitution . . . . . . . . . . . . . . ?? Ben Short The Tudor family produces heirs . . . . ?? Ben Short The wrong suspect? . . . . . . . . . . . 707 Caitlin Sedwick Joan Steitz: RNA is a many-splendored thing . . . . . . . . . . . . . . . . . 708 Indika Rajapakse and Mark Groudine On emerging nuclear order . . . . . . . 711 Christophe Zimmer and Emmanuelle Fabre Principles of chromosomal organization: lessons from yeast . . . . . . . . . . . 723 Sheba Agarwal and Wiggert A. van Cappellen and Aude Guénolé and Berina Eppink and Sam E. V. Linsen and Erik Meijering and Adriaan Houtsmuller and Roland Kanaar and Jeroen Essers ATP-dependent and independent functions of Rad54 in genome maintenance . . . . . 735 Antoine Bondue and Simon Tännler and Giuseppe Chiapparo and Samira Chabab and Mirana Ramialison and Catherine Paulissen and Benjamin Beck and Richard Harvey and Cédric Blanpain Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation . . . . . . . . . . . . 751 François Gerbe and Johan H. van Es and Leila Makrini and Bénédicte Brulin and Georg Mellitzer and Sylvie Robine and Béatrice Romagnolo and Noah F. Shroyer and Jean-François Bourgaux and Christine Pignodel and Hans Clevers and Philippe Jay Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium 767 Yukihiro Yabuta and Hiroshi Ohta and Takaya Abe and Kazuki Kurimoto and Shinichiro Chuma and Mitinori Saitou TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice . . . . . . . . . 781 Lisbeth S. Laursen and Colin W. Chan and Charles ffrench-Constant Translation of myelin basic protein mRNA in oligodendrocytes is regulated by integrin activation and hnRNP--K . . . . 797 Hél\`ene Vacher and Jae-Won Yang and Oscar Cerda and Amapola Autillo-Touati and Bénédicte Dargent and James S. Trimmer Cdk-mediated phosphorylation of the Kv$ \beta $2 auxiliary subunit regulates Kv1 channel axonal targeting . . . . . . . . 813 Xin Chen and Hanna Tukachinsky and Chih-Hsiang Huang and Cindy Jao and Yue-Ru Chu and Hsiang-Yun Tang and Britta Mueller and Sol Schulman and Tom A. Rapoport and Adrian Salic Processing and turnover of the Hedgehog protein in the endoplasmic reticulum . . 825 Takashi Itoh and Eiko Kanno and Takefumi Uemura and Satoshi Waguri and Mitsunori Fukuda OATL1, a novel autophagosome-resident Rab33B--GAP, regulates autophagosomal maturation . . . . . . . . . . . . . . . 839 Stéphanie Bolhy and Im\`ene Bouhlel and Elisa Dultz and Tania Nayak and Michela Zuccolo and Xavier Gatti and Richard Vallee and Jan Ellenberg and Valérie Doye A Nup133-dependent NPC-anchored network tethers centrosomes to the nuclear envelope in prophase . . . . . . . . . . 855 Won-Seok Choi and Richard D. Palmiter and Zhengui Xia Loss of mitochondrial complex I activity potentiates dopamine neuron death induced by microtubule dysfunction in a Parkinson's disease model . . . . . . . 873 Sarah Schmidt and Inaam Nakchbandi and Raphael Ruppert and Nina Kawelke and Michael W. Hess and Kristian Pfaller and Pierre Jurdic and Reinhard Fässler and Markus Moser Kindlin-3-mediated signaling from multiple integrin classes is required for osteoclast-mediated bone resorption 883 Rafael Bernad and Patricia Sánchez and Teresa Rivera and Miriam Rodríguez-Corsino and Ekaterina Boyarchuk and Isabelle Vassias and Dominique Ray-Gallet and Alexei Arnaoutov and Mary Dasso and Genevi\`eve Almouzni and Ana Losada Xenopus HJURP and condensin II are required for CENP--A assembly . . . . . 899
Ben Short Location is everything . . . . . . . . . ?? Ben Short Connecting the cytokinesis dots . . . . ?? Ben Short Osteoblasts are bone idle without Frizzled-9 . . . . . . . . . . . . . . . ?? Ben Short Cadherins trade bonds . . . . . . . . . 903 Caitlin Sedwick Pascale Cossart: The ins and outs of \bionameListeria . . . . . . . . . . . . 904 Buzz Baum and Marios Georgiou Dynamics of adherens junctions in epithelial establishment, maintenance, and remodeling . . . . . . . . . . . . . 907 Shannon Behrman and Diego Acosta-Alvear and Peter Walter A CHOP-regulated microRNA controls rhodopsin expression . . . . . . . . . . 919 Momoyo Hanazawa and Masafumi Yonetani and Asako Sugimoto PGL proteins self associate and bind RNPs to mediate germ granule assembly in \bionameC. elegans . . . . . . . . . . . 929 Dustin L. Updike and Stephanie J. Hachey and Jeremy Kreher and Susan Strome P granules extend the nuclear pore complex environment in the \bionameC. elegans germ line . . . . . . . . . . . 939 Damien Laporte and Anne Lebaudy and Annelise Sahin and Beno\^\it Pinson and Johanna Ceschin and Bertrand Daignan-Fornier and Isabelle Sagot Metabolic status rather than cell cycle signals control quiescence entry and exit . . . . . . . . . . . . . . . . . . 949 Anja K. Dunsch and Emily Linnane and Francis A. Barr and Ulrike Gruneberg The astrin--kinastrin/SKAP complex localizes to microtubule plus ends and facilitates chromosome alignment . . . . 959 Juan A. Bernal and Ashok R. Venkitaraman A vertebrate N-end rule degron reveals that Orc6 is required in mitosis for daughter cell abscission . . . . . . . . 969 Bill X. Huang and Mohammed Akbar and Karl Kevala and Hee-Yong Kim Phosphatidylserine is a critical modulator for Akt activation . . . . . . 979 Yoshimi Endo Greer and Jeffrey S. Rubin Casein kinase 1 delta functions at the centrosome to mediate Wnt-3a-dependent neurite outgrowth . . . . . . . . . . . 993 Damien Laporte and Valerie C. Coffman and I-Ju Lee and Jian-Qiu Wu Assembly and architecture of precursor nodes during fission yeast cytokinesis 1005 Corey L. Williams and Chunmei Li and Katarzyna Kida and Peter N. Inglis and Swetha Mohan and Lucie Semenec and Nathan J. Bialas and Rachel M. Stupay and Nansheng Chen and Oliver E. Blacque and Bradley K. Yoder and Michel R. Leroux MKS and NPHP modules cooperate to establish basal body/transition zone membrane associations and ciliary gate function during ciliogenesis . . . . . . 1023 Oludotun Adeyo and Patrick J. Horn and SungKyung Lee and Derk D. Binns and Anita Chandrahas and Kent D. Chapman and Joel M. Goodman The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets . . . . . . . . . . . . . . . . 1043 Joachim Albers and Jochen Schulze and F. Timo Beil and Matthias Gebauer and Anke Baranowsky and Johannes Keller and Robert P. Marshall and Kristofer Wintges and Felix W. Friedrich and Matthias Priemel and Arndt F. Schilling and Johannes M. Rueger and Kerstin Cornils and Boris Fehse and Thomas Streichert and Guido Sauter and Franz Jakob and Karl L. Insogna and Barbara Pober and Klaus-Peter Knobeloch and Uta Francke and Michael Amling and Thorsten Schinke Control of bone formation by the serpentine receptor Frizzled-9 . . . . . 1057 Soonjin Hong and Regina B. Troyanovsky and Sergey M. Troyanovsky Cadherin exits the junction by switching its adhesive bond . . . . . . . . . . . 1073
Mitch Leslie Paraspeckles may provide stress relief ?? Mitch Leslie Multiple routes to the inner nuclear membrane . . . . . . . . . . . . . . . . ?? Mitch Leslie Wnt sends mixed signals in the skin . . ?? Mitch Leslie Kinetochores feel the pull . . . . . . . 3 Caitlin Sedwick Peter Walter: Investigating how the ER handles secretory proteins . . . . . . . 4 Changle Ma and Gaurav Agrawal and Suresh Subramani Peroxisome assembly: matrix and membrane protein biogenesis . . . . . . . . . . . 7 Frank Sicheri and Robert H. Silverman Putting the brakes on the unfolded protein response . . . . . . . . . . . . 17 Izhak Kehat and Federica Accornero and Bruce J. Aronow and Jeffery D. Molkentin Modulation of chromatin position and gene expression by HDAC4 interaction with nucleoporins . . . . . . . . . . . 21 Shinichi Nakagawa and Takao Naganuma and Go Shioi and Tetsuro Hirose Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice . . . . . . . . . . . . . . . . . . 31 Aditi Chawla and Sutapa Chakrabarti and Gourisankar Ghosh and Maho Niwa Attenuation of yeast UPR is essential for survival and is mediated by IRE1 kinase . . . . . . . . . . . . . . . . . 41 Richard S. Kang and Heike Fölsch ARH cooperates with AP-1B in the exocytosis of LDLR in polarized epithelial cells . . . . . . . . . . . . 51 Zhizhan Gu and Erika H. Noss and Victor W. Hsu and Michael B. Brenner Integrins traffic rapidly via circular dorsal ruffles and macropinocytosis during stimulated cell migration . . . . 61 Tobias Morawe and Mona Honemann-Capito and Walter von Stein and Andreas Wodarz Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in \bionameDrosophila oogenesis 71 Céline Baldeyron and Gaston Soria and Dani\`ele Roche and Adam J. L. Cook and Genevi\`eve Almouzni HP1$ \alpha $ recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair . . . . . . . . . . 81 Jeanine A. Harrigan and Rimma Belotserkovskaya and Julia Coates and Daniela S. Dimitrova and Sophie E. Polo and Charles R. Bradshaw and Peter Fraser and Stephen P. Jackson Replication stress induces 53BP1-containing OPT domains in G1 cells 97 Nikolaj Zuleger and David A. Kelly and A. Christine Richardson and Alastair R. W. Kerr and Martin W. Goldberg and Andrew B. Goryachev and Eric C. Schirmer System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics . . . 109 Aussie Suzuki and Tetsuya Hori and Tatsuya Nishino and Jiro Usukura and Atsushi Miyagi and Kosuke Morikawa and Tatsuo Fukagawa Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins . . . . . . . . . . . . . . . . 125 Elise Peyre and Florence Jaouen and Mehdi Saadaoui and Laurence Haren and Andreas Merdes and Pascale Durbec and Xavier Morin A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells 141 Lindsay Lewellyn and Ana Carvalho and Arshad Desai and Amy S. Maddox and Karen Oegema The chromosomal passenger complex and centralspindlin independently contribute to contractile ring assembly . . . . . . 155 Claudia Rubio and David Pincus and Alexei Korennykh and Sebastian Schuck and Hana El-Samad and Peter Walter Homeostatic adaptation to endoplasmic reticulum stress depends on Ire1 kinase activity . . . . . . . . . . . . . . . . 171 Jenna L. Jewell and Eunjin Oh and Latha Ramalingam and Michael A. Kalwat and Vincent S. Tagliabracci and Lixuan Tackett and Jeffrey S. Elmendorf and Debbie C. Thurmond Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis . . . . . . . . . . 185 Avital A. Rodal and Aline D. Blunk and Yulia Akbergenova and Ramon A. Jorquera and Lauren K. Buhl and J. Troy Littleton A presynaptic endosomal trafficking pathway controls synaptic growth signaling . . . . . . . . . . . . . . . 201 Stephen S. Francis and Jeff Sfakianos and Bryan Lo and Ira Mellman A hierarchy of signals regulates entry of membrane proteins into the ciliary membrane domain in epithelial cells . . 219 Karen M. Osorio and Karin C. Lilja and Tudorita Tumbar Runx1 modulates adult hair follicle stem cell emergence and maintenance from distinct embryonic skin compartments . . 235
Ben Short Passengers operate the brakes . . . . . ?? Ben Short How the ER shapes up and ships out . . . ?? Ben Short Myosin II gets polarity back to front ?? Ben Short Repair defects put stem cells in a fix 253 Ben Short Angelika Amon: Conquering the divide . . 254 Ling Liu and Thomas A. Rando Manifestations and mechanisms of stem cell aging . . . . . . . . . . . . . . . 257 Jiadong Wang and Zihua Gong and Junjie Chen MDC1 collaborates with TopBP1 in DNA replication checkpoint control . . . . . 267 Yoshihiro Inami and Satoshi Waguri and Ayako Sakamoto and Tsuguka Kouno and Kazuto Nakada and Okio Hino and Sumio Watanabe and Jin Ando and Manabu Iwadate and Masayuki Yamamoto and Myung-Shik Lee and Keiji Tanaka and Masaaki Komatsu Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells 275 Daniel K. Rozelle and Scott D. Hansen and Kenneth B. Kaplan Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake . . . . . . . . . 285 Shichuan Zhang and Hirohiko Yajima and HoangDinh Huynh and Junke Zheng and Elsa Callen and Hua-Tang Chen and Nancy Wong and Samuel Bunting and Yu-Fen Lin and Mengxia Li and Kyung-Jone Lee and Michael Story and Eric Gapud and Barry P. Sleckman and André Nussenzweig and Cheng Cheng Zhang and David J. Chen and Benjamin P. C. Chen Congenital bone marrow failure in DNA--PKcs mutant mice associated with deficiencies in DNA repair . . . . . . . 295 Tiago J. Dantas and Yifan Wang and Pierce Lalor and Peter Dockery and Ciaran G. Morrison Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins . . . . . . . . . . 307 Tomoki Yano and Yuji Yamazaki and Makoto Adachi and Katsuya Okawa and Philippe Fort and Masami Uji and Shoichiro Tsukita and Sachiko Tsukita Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling . . 319 Matt West and Nesia Zurek and Andreas Hoenger and Gia K. Voeltz A $3$D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature . . . . . . . . . . . 333 Michele E. Day and Guido M. Gaietta and Mira Sastri and Antonius Koller and Mason R. Mackey and John D. Scott and Guy A. Perkins and Mark H. Ellisman and Susan S. Taylor Isoform-specific targeting of PKA to multivesicular bodies . . . . . . . . . 347 Tai Kiuchi and Tomoaki Nagai and Kazumasa Ohashi and Kensaku Mizuno Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension . . 365 Miguel Vicente-Manzanares and Karen Newell-Litwa and Alexia I. Bachir and Leanna A. Whitmore and Alan Rick Horwitz Myosin IIA/IIB restrict adhesive and protrusive signaling to generate front--back polarity in migrating cells 381 Céline Caillava and Renaud Vandenbosch and Beata Jablonska and Cyrille Deboux and Giulia Spigoni and Vittorio Gallo and Brigitte Malgrange and Anne Baron-Van Evercooren Cdk2 loss accelerates precursor differentiation and remyelination in the adult central nervous system . . . . . . 397 Dan Xu and Fumitaka Takeshita and Yumiko Hino and Saori Fukunaga and Yasusei Kudo and Aya Tamaki and Junko Matsunaga and Ryou-u Takahashi and Takashi Takata and Akira Shimamoto and Takahiro Ochiya and Hidetoshi Tahara miR-22 represses cancer progression by inducing cellular senescence . . . . . . 409
Mitch Leslie Proteins keep Cdc55 in its place . . . . ?? Mitch Leslie A histone finds a traveling companion ?? Mitch Leslie Plant virus gets the bends . . . . . . . ?? Mitch Leslie PAR proteins cross the boundary . . . . 427 Caitlin Sedwick Erik Sahai: Getting the whole picture of metastasis . . . . . . . . . . . . . . . 428 Hans A. Kestler and Michael Kühl Generating a Wnt switch: it's all about the right dosage . . . . . . . . . . . . 431 Tetsuo Kobayashi and Brian D. Dynlacht Regulating the transition from centriole to basal body . . . . . . . . . . . . . 435 Valentina Rossio and Satoshi Yoshida Spatial regulation of Cdc55--PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast . . . . . 445 Maria Teresa Abreu-Blanco and Jeffrey M. Verboon and Susan M. Parkhurst Cell wound repair in \bionameDrosophila occurs through three distinct phases of membrane and cytoskeletal remodeling . . 455 Peter J. Cavnar and Erwin Berthier and David J. Beebe and Anna Huttenlocher Hax1 regulates neutrophil adhesion and motility through RhoA . . . . . . . . . 465 He Zhang and Beibei Niu and Ji-Fan Hu and Shengfang Ge and Haibo Wang and Tao Li and Jianqun Ling and Brandon N. Steelman and Guanxiang Qian and Andrew R. Hoffman Interruption of intrachromosomal looping by CCCTC binding factor decoy proteins abrogates genomic imprinting of human insulin-like growth factor II . . . . . 475 Jennifer M. Gardner and Christine J. Smoyer and Elizabeth S. Stensrud and Richard Alexander and Madelaine Gogol and Winfried Wiegraebe and Sue L. Jaspersen Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z . . . . . . . . . . . . . 489 Jung-Chun Lin and Woan-Yuh Tarn RBM4 down-regulates PTB and antagonizes its activity in muscle cell--specific alternative splicing . . . . . . . . . . 509 Chih-Hang Wu and Shu-Chuan Lee and Chao-Wen Wang Viral protein targeting to the cortical endoplasmic reticulum is required for cell--cell spreading in plants . . . . . 521 Ilaria Prada and Julie Marchaland and Paola Podini and Lorenzo Magrassi and Rosalba D'Alessandro and Paola Bezzi and Jacopo Meldolesi REST\slash NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes . . . . . . . . . . . . . . . 537 Giovanna Nalesso and Joanna Sherwood and Jessica Bertrand and Thomas Pap and Manoj Ramachandran and Cosimo De Bari and Costantino Pitzalis and Francesco Dell'Accio WNT-3A modulates articular chondrocyte phenotype by activating both canonical and noncanonical pathways . . . . . . . 551 David R. Raleigh and Devin M. Boe and Dan Yu and Christopher R. Weber and Amanda M. Marchiando and Emily M. Bradford and Yingmin Wang and Licheng Wu and Eveline E. Schneeberger and Le Shen and Jerrold R. Turner Occludin S408 phosphorylation regulates tight junction protein interactions and barrier function . . . . . . . . . . . . 565 Nathan W. Goehring and Carsten Hoege and Stephan W. Grill and Anthony A. Hyman PAR proteins diffuse freely across the anterior--posterior boundary in polarized \bionameC. elegans embryos . . 583
Ben Short HIV-1 makes a pore adjustment . . . . . ?? Ben Short Sphingolipid puts the squeeze on apoptotic cells . . . . . . . . . . . . ?? Ben Short Bigger isn't better . . . . . . . . . . ?? Ben Short Titin isn't a sleeping giant . . . . . . 597 Ben Short Harvey McMahon: Ahead of the curve on membrane dynamics . . . . . . . . . . . 598 Michael S. Brown and Joseph L. Goldstein Richard G.W. Anderson (1940--2011) and the birth of receptor-mediated endocytosis . . . . . . . . . . . . . . 601 Miroslav Dundr Seed and grow: a two-step model for nuclear body biogenesis . . . . . . . . 605 Stefan Schulte-Merker and Amélie Sabine and Tatiana V. Petrova Lymphatic vascular morphogenesis in development, physiology, and disease . . 607 Anne Monette and Nelly Panté and Andrew J. Mouland HIV-1 remodels the nuclear pore complex 619 Sandra Habbig and Malte P. Bartram and Roman U. Müller and Ricarda Schwarz and Nikolaos Andriopoulos and Shuhua Chen and Josef G. Sägmüller and Martin Hoehne and Volker Burst and Max C. Liebau and H. Christian Reinhardt and Thomas Benzing and Bernhard Schermer NPHP4, a cilia-associated protein, negatively regulates the Hippo pathway 633 Yingke Xu and Bradley R. Rubin and Charisse M. Orme and Alexander Karpikov and Chenfei Yu and Jonathan S. Bogan and Derek K. Toomre Dual-mode of insulin action controls GLUT4 vesicle exocytosis . . . . . . . . 643 Francisco M. Vega and Gilbert Fruhwirth and Tony Ng and Anne J. Ridley RhoA and RhoC have distinct roles in migration and invasion by acting through different targets . . . . . . . . . . . 655 Yapeng Gu and Tetyana Forostyan and Roger Sabbadini and Jody Rosenblatt Epithelial cell extrusion requires the sphingosine-$1$-phosphate receptor 2 pathway . . . . . . . . . . . . . . . . 667 Anne E. White and Brandon D. Burch and Xiao-cui Yang and Pamela Y. Gasdaska and Zbigniew Dominski and William F. Marzluff and Robert J. Duronio Drosophila histone locus bodies form by hierarchical recruitment of components 677 Alla Amcheslavsky and Naoto Ito and Jin Jiang and Y. Tony Ip Tuberous sclerosis complex and Myc coordinate the growth and division of \bionameDrosophila intestinal stem cells 695 Radhika Gudi and Chaozhong Zou and Jun Li and Qingshen Gao Centrobin--tubulin interaction is required for centriole elongation and stability . . . . . . . . . . . . . . . 711 Won-Jing Wang and Rajesh Kumar Soni and Kunihiro Uryu and Meng-Fu Bryan Tsou The conversion of centrioles to centrosomes: essential coupling of duplication with segregation . . . . . . 727 Telsa M. Mittelmeier and Joseph S. Boyd and Mary Rose Lamb and Carol L. Dieckmann Asymmetric properties of the Chlamydomonas reinhardtii cytoskeleton direct rhodopsin photoreceptor localization . . . . . . . . . . . . . . 741 Kai Mao and Ke Wang and Mantong Zhao and Tao Xu and Daniel J. Klionsky Two MAPK-signaling pathways are required for mitophagy in \bionameSaccharomyces cerevisiae . . . . . . . . . . . . . . . 755 Chiung-Ya Chen and Chia-Wen Lin and Chiung-Ying Chang and Si-Tse Jiang and Yi-Ping Hsueh Sarm1, a negative regulator of innate immunity, interacts with syndecan-2 and regulates neuronal morphology . . . . . 769 Katharina da Silva Lopes and Agnieszka Pietas and Michael H. Radke and Michael Gotthardt Titin visualization in real time reveals an unexpected level of mobility within and between sarcomeres . . . . . . . . . 785
Mitch Leslie WASH cleans up after sloppy eaters . . . ?? Mitch Leslie Caveolin-1 says NO to permeability . . . ?? Mitch Leslie Noncoding RNA to blame for bad editing ?? Mitch Leslie One collagen shipment, ready for delivery . . . . . . . . . . . . . . . . 801 Caitlin Sedwick Valerie Weaver: Overcoming cancer's stiff resistance . . . . . . . . . . . . 802 Wakako Takabe and Noah Alberts-Grill and Hanjoong Jo Disturbed flow: p53 SUMOylation in the turnover of endothelial cells . . . . . 805 Chan Bae Park and Nils-Göran Larsson Mitochondrial DNA mutations in disease and aging . . . . . . . . . . . . . . . 809 Ute Schmidt and Eugenia Basyuk and Marie-Cécile Robert and Minoru Yoshida and Jean-Philippe Villemin and Didier Auboeuf and Stuart Aitken and Edouard Bertrand Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation . . . . 819 Michael Carnell and Tobias Zech and Simon D. Calaminus and Seiji Ura and Monica Hagedorn and Simon A. Johnston and Robin C. May and Thierry Soldati and Laura M. Machesky and Robert H. Insall Actin polymerization driven by WASH causes V--ATPase retrieval and vesicle neutralization before exocytosis . . . . 831 M. Rizwan Siddiqui and Yulia A. Komarova and Stephen M. Vogel and Xiaopei Gao and Marcelo G. Bonini and Johnson Rajasingh and You-Yang Zhao and Viktor Brovkovych and Asrar B. Malik Caveolin-1-eNOS signaling promotes p190RhoGAP--A nitration and endothelial permeability . . . . . . . . . . . . . . 841 Sara Massone and Irene Vassallo and Manuele Castelnuovo and Gloria Fiorino and Elena Gatta and Mauro Robello and Roberta Borghi and Massimo Tabaton and Claudio Russo and Giorgio Dieci and Ranieri Cancedda and Aldo Pagano RNA polymerase III drives alternative splicing of the potassium channel--interacting protein contributing to brain complexity and neurodegeneration . . . . . . . . . . . 851 Kyung-Sun Heo and Hakjoo Lee and Patrizia Nigro and Tamlyn Thomas and Nhat-Tu Le and Eugene Chang and Carolyn McClain and Cynthia A. Reinhart-King and Michael R. King and Bradford C. Berk and Keigi Fujiwara and Chang-Hoon Woo and Jun-ichi Abe PKC$ \zeta $ mediates disturbed flow-induced endothelial apoptosis via p53 SUMOylation . . . . . . . . . . . . 867 Ruchi Saraya and Arjen M. Krikken and Marten Veenhuis and Ida J. van der Klei Peroxisome reintroduction in Hansenula polymorpha requires Pex25 and Rho1 . . . 885 Junya Hasegawa and Emi Tokuda and Takeshi Tenno and Kazuya Tsujita and Haruko Sawai and Hidekazu Hiroaki and Tadaomi Takenawa and Toshiki Itoh SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain . . . . . . . . . . . . . . . . . 901 Lidia Hurtado and Cristina Caballero and Maria P. Gavilan and Jesus Cardenas and Michel Bornens and Rosa M. Rios Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis . . . . . . . 917 Deanna G. Wilson and Khanhky Phamluong and Li Li and Mei Sun and Tim C. Cao and Peter S. Liu and Zora Modrusan and Wendy N. Sandoval and Linda Rangell and Richard A. D. Carano and Andrew S. Peterson and Mark J. Solloway Global defects in collagen secretion in a Mia3/TANGO1 knockout mouse . . . . . . 935
Ben Short Cdks put Treslin on TopBP1 . . . . . . . ?? Ben Short Epac1 cAMPs out at nuclear pores . . . . ?? Ben Short Aurora A limits calcium release . . . . ?? Ben Short New TIPs for successfully growing microtubules . . . . . . . . . . . . . . 955 Caitlin Sedwick David Bilder: Getting to know epithelia inside and out . . . . . . . . . . . . . 956 Helge Ewers Septin pairs, a complex choreography . . 959 Mark C. Field and Andrej Sali and Michael P. Rout On a bender --- BARs, ESCRTs, COPs, and finally getting your coat . . . . . . . 963 Wenjing Li and Tomohiro Miki and Takashi Watanabe and Mai Kakeno and Ikuko Sugiyama and Kozo Kaibuchi and Gohta Goshima EB1 promotes microtubule dynamics by recruiting Sentin in \bionameDrosophila cells . . . . . . . . . . . . . . . . . 973 Mei-Yao Lin and Yu-Min Lin and Te-chan Kao and Hsiang-Hao Chuang and Ruey-Hwa Chen PDZ-RhoGEF ubiquitination by Cullin3--KLHL20 controls neurotrophin-induced neurite outgrowth 985 Akiko Kumagai and Anna Shevchenko and Andrej Shevchenko and William G. Dunphy Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication . . . . . . 995 Martijn Gloerich and Marjolein J. Vliem and Esther Prummel and Lars A. T. Meijer and Marije G. A. Rensen and Holger Rehmann and Johannes L. Bos The nucleoporin RanBP2 tethers the cAMP effector Epac1 and inhibits its catalytic activity . . . . . . . . . . . 1009 Olga V. Plotnikova and Elena N. Pugacheva and Erica A. Golemis Aurora A kinase activity influences calcium signaling in kidney cells . . . 1021 Daniela Trinca Bertazzi and Bahtiyar Kurtulmus and Gislene Pereira The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4 . . . . 1033 Robin M. Ricke and Karthik B. Jeganathan and Jan M. van Deursen Bub1 overexpression induces aneuploidy and tumor formation through Aurora B kinase hyperactivation . . . . . . . . . 1049 Bradley S. DeMay and Xiaobo Bai and Louisa Howard and Patricia Occhipinti and Rebecca A. Meseroll and Elias T. Spiliotis and Rudolf Oldenbourg and Amy S. Gladfelter Septin filaments exhibit a dynamic, paired organization that is conserved from yeast to mammals . . . . . . . . . 1065 Babet van der Vaart and Cristina Manatschal and Ilya Grigoriev and Vincent Olieric and Susana Montenegro Gouveia and Sa\vsa Bjeli\'c and Jeroen Demmers and Ivan Vorobjev and Casper C. Hoogenraad and Michel O. Steinmetz and Anna Akhmanova SLAIN2 links microtubule plus end--tracking proteins and controls microtubule growth in interphase . . . . 1083 Steffi Gruschke and Kirsten Kehrein and Katharina Römpler and Kerstin Gröne and Lars Israel and Axel Imhof and Johannes M. Herrmann and Martin Ott Cbp3--Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly . . . . . . . . . . . . . . . . 1101 Yayoi Izu and Mei Sun and Daniela Zwolanek and Guido Veit and Valerie Williams and Byeong Cha and Karl J. Jepsen and Manuel Koch and David E. Birk Type XII collagen regulates osteoblast polarity and communication during bone formation . . . . . . . . . . . . . . . 1115
Ben Short Retinoic acid keeps Lingo-1 quiet . . . ?? Ben Short Cohesin and condensin spring into action ?? Ben Short miRNAs prevent a change of heart . . . . ?? Ben Short Cdc42 and Tinman march to the same beat 1133 Caitlin Sedwick Jagesh Shah: a quantitative approach to cell biology . . . . . . . . . . . . . . 1134 Hiroki Oda and Masatoshi Takeichi Structural and functional diversity of cadherin at the adherens junction . . . 1137 Radhika Puttagunta and André Schmandke and Elisa Floriddia and Perrine Gaub and Natalie Fomin and Norbert B. Ghyselinck and Simone Di Giovanni RA-RAR-$ \beta $ counteracts myelin-dependent inhibition of neurite outgrowth via Lingo-1 repression . . . . 1147 Gary W. Kerr and Sourav Sarkar and Katherine L. Tibbles and Mark Petronczki and Jonathan B. A. Millar and Prakash Arumugam Meiotic nuclear divisions in budding yeast require PP2A$^{\rm Cdc55}$-mediated antagonism of Net1 phosphorylation by Cdk . . . . . . . . . 1157 Andrew D. Stephens and Julian Haase and Leandra Vicci and Russell M. Taylor and Kerry Bloom Cohesin, condensin, and the intramolecular centromere loop together generate the mitotic chromatin spring 1167 Li Qian and Joshua D. Wythe and Jiandong Liu and Jerome Cartry and Georg Vogler and Bhagyalaxmi Mohapatra and Robyn T. Otway and Yu Huang and Isabelle N. King and Marjorie Maillet and Yi Zheng and Timothy Crawley and Ouarda Taghli-Lamallem and Christopher Semsarian and Sally Dunwoodie and David Winlaw and Richard P. Harvey and Diane Fatkin and Jeffrey A. Towbin and Jeffery D. Molkentin and Deepak Srivastava and Karen Ocorr and Benoit G. Bruneau and Rolf Bodmer Tinman\slash Nkx2-5 acts via miR-1 and upstream of Cdc42 to regulate heart function across species . . . . . . . . 1181 Stefania Crippa and Marco Cassano and Graziella Messina and Daniela Galli and Beatriz G. Galvez and Tomaz Curk and Claudia Altomare and Flavio Ronzoni and Jaan Toelen and Rik Gijsbers and Zeger Debyser and Stefan Janssens and Blaz Zupan and Antonio Zaza and Giulio Cossu and Maurilio Sampaolesi miR669a and miR669q prevent skeletal muscle differentiation in postnatal cardiac progenitors . . . . . . . . . . 1197 Farid Bizzari and Adele L. Marston Cdc55 coordinates spindle assembly and chromosome disjunction during meiosis 1213 Marina L. Ellefson and Francis J. McNally CDK-1 inhibits meiotic spindle shortening and dynein-dependent spindle rotation in \bionameC. elegans . . . . . 1229 Jun Zhang and Xuanli Yao and Lauren Fischer and Juan F. Abenza and Miguel A. Peñalva and Xin Xiang The p25 subunit of the dynactin complex is required for dynein--early endosome interaction . . . . . . . . . . . . . . 1245 Masaki Ishikawa and Tsutomu Iwamoto and Takashi Nakamura and Andrew Doyle and Satoshi Fukumoto and Yoshihiko Yamada Pannexin 3 functions as an ER Ca$^{2+}$ channel, hemichannel, and gap junction to promote osteoblast differentiation 1257 Hideki Yamaguchi and Shuhei Yoshida and Emi Muroi and Nachi Yoshida and Masahiro Kawamura and Zen Kouchi and Yoshikazu Nakamura and Ryuichi Sakai and Kiyoko Fukami Phosphoinositide $3$-kinase signaling pathway mediated by p110$ \alpha $ regulates invadopodia formation . . . . 1275 Violaine D. Delorme-Walker and Jeffrey R. Peterson and Jonathan Chernoff and Clare M. Waterman and Gaudenz Danuser and Céline DerMardirossian and Gary M. Bokoch Pak1 regulates focal adhesion strength, myosin IIA distribution, and actin dynamics to optimize cell migration . . 1289 Lee B. Rivera and Rolf A. Brekken SPARC promotes pericyte recruitment via inhibition of endoglin-dependent TGF-$ \beta $1 activity . . . . . . . . . . . 1305
Mitch Leslie New Twist on cell migration . . . . . . ?? Mitch Leslie Bak activators get in the groove . . . . ?? Mitch Leslie SUMO defeats protein aggregates . . . . ?? Mitch Leslie The sarcoplasmic reticulum takes on a supporting role . . . . . . . . . . . . 3 Caitlin Sedwick Carol Dieckmann: an eye on organellar biology . . . . . . . . . . . . . . . . 4 Toren Finkel Signal transduction by reactive oxygen species . . . . . . . . . . . . . . . . 7 Rachel Lander and Kara Nordin and Carole LaBonne The F-box protein Ppa is a common regulator of core EMT factors Twist, Snail, Slug, and Sip1 . . . . . . . . . 17 Jessica A. Talamas and Martin W. Hetzer POM121 and Sun1 play a role in early steps of interphase NPC assembly . . . . 27 Haiming Dai and Alyson Smith and X. Wei Meng and Paula A. Schneider and Yuan-Ping Pang and Scott H. Kaufmann Transient binding of an activator BH3 domain to the Bak BH3-binding groove initiates Bak oligomerization . . . . . 39 Petranka Krumova and Erik Meulmeester and Manuel Garrido and Marilyn Tirard and He-Hsuan Hsiao and Guillaume Bossis and Henning Urlaub and Markus Zweckstetter and Sebastian Kügler and Frauke Melchior and Mathias Bähr and Jochen H. Weishaupt Sumoylation inhibits $ \alpha $-synuclein aggregation and toxicity . . 49 Morihisa Fujita and Reika Watanabe and Nina Jaensch and Maria Romanova-Michaelides and Tadashi Satoh and Masaki Kato and Howard Riezman and Yoshiki Yamaguchi and Yusuke Maeda and Taroh Kinoshita Sorting of GPI-anchored proteins into ER exit sites by p24 proteins is dependent on remodeled GPI . . . . . . . . . . . . 61 Dinari A. Harris and Kevin Kim and Kenji Nakahara and Constanza Vásquez-Doorman and Richard W. Carthew Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing . . . . . . . . . . . . . . . 77 Iwona M. Pranke and Vincent Morello and Joëlle Bigay and Kimberley Gibson and Jean-Marc Verbavatz and Bruno Antonny and Catherine L. Jackson $ \alpha $-Synuclein and ALPS motifs are membrane curvature sensors whose contrasting chemistry mediates selective vesicle binding . . . . . . . . . . . . 89 David S. Gokhin and Velia M. Fowler Cytoplasmic $ \gamma $-actin and tropomodulin isoforms link to the sarcoplasmic reticulum in skeletal muscle fibers . . . . . . . . . . . . . 105 Richard M. Parton and Russell S. Hamilton and Graeme Ball and Lei Yang and C. Fiona Cullen and Weiping Lu and Hiroyuki Ohkura and Ilan Davis A PAR-1-dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the \bionameDrosophila oocyte . . . . . . . 121 Yuko Nakajima and Anthony Cormier and Randall G. Tyers and Adrianne Pigula and Yutian Peng and David G. Drubin and Georjana Barnes Ipl1\slash Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC--spindle interaction to ensure proper microtubule dynamics . . . 137 Steffi Gruschke and Kirsten Kehrein and Katharina Römpler and Kerstin Gröne and Lars Israel and Axel Imhof and Johannes M. Herrmann and Martin Ott Cbp3--Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly . . . . . . . . . . . . . . . . 155 Olga V. Plotnikova and Elena N. Pugacheva and Erica A. Golemis Aurora A kinase activity influences calcium signaling in kidney cells . . . 157
Ben Short AMPK amplifies Huntington's disease . . ?? Ben Short HJURP puts the centromere in place . . . ?? Ben Short Sphingomyelinase helps bones get their minerals . . . . . . . . . . . . . . . . ?? Ben Short Microtubules follow septins' guidelines 161 Caitlin Sedwick Rüdiger Klein: Reading the guideposts for axon guidance . . . . . . . . . . . . . 162 Zita Carvalho-Santos and Juliette Azimzadeh and José. B. Pereira-Leal and Mónica Bettencourt-Dias Tracing the origins of centrioles, cilia, and flagella . . . . . . . . . . 165 Xiomaris M. Cotto-Rios and Mathew J. K. Jones and Luca Busino and Michele Pagano and Tony T. Huang APC/C$^{\rm Cdh1}$-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage . . . . . . . . . 177 Jonathan R. Bowen and Daniel Hwang and Xiaobo Bai and Dheeraj Roy and Elias T. Spiliotis Septin GTPases spatially guide microtubule organization and plus end dynamics in polarizing epithelia . . . . 187 Anna Krzywicka-Racka and Greenfield Sluder Repeated cleavage failure does not establish centrosome amplification in untransformed human cells . . . . . . . 199 Tz-Chuen Ju and Hui-Mei Chen and Jiun-Tsai Lin and Ching-Pang Chang and Wei-Cheng Chang and Jheng-Jie Kang and Cheng-Pu Sun and Mi-Hua Tao and Pang-Hsien Tu and Chen Chang and Dennis W. Dickson and Yijuang Chern Nuclear translocation of AMPK-$ \alpha $1 potentiates striatal neurodegeneration in Huntington's disease . . . . . . . . . . . . . . . . 209 Meghan C. Barnhart and P. Henning J. L. Kuich and Madison E. Stellfox and Jared A. Ward and Emily A. Bassett and Ben E. Black and Daniel R. Foltz HJURP is a CENP--A chromatin assembly factor sufficient to form a functional de novo kinetochore . . . . . . . . . . 229 Takao Nakata and Shinsuke Niwa and Yasushi Okada and Franck Perez and Nobutaka Hirokawa Preferential binding of a kinesin-1 motor to GTP-tubulin--rich microtubules underlies polarized vesicle transport 245 Gregory D. Fairn and Nicole L. Schieber and Nicholas Ariotti and Samantha Murphy and Lars Kuerschner and Richard I. Webb and Sergio Grinstein and Robert G. Parton High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine . . . . . . . . . . . 257 Zohreh Khavandgar and Christophe Poirier and Christopher J. Clarke and Jingjing Li and Nicholas Wang and Marc D. McKee and Yusuf A. Hannun and Monzur Murshed A cell-autonomous requirement for neutral sphingomyelinase 2 in bone mineralization . . . . . . . . . . . . . 277 Anja Mai and Stefan Veltel and Teijo Pellinen and Artur Padzik and Eleanor Coffey and Varpu Marjomäki and Johanna Ivaska Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration . . . . . 291 Molly Brunner and Angélique Millon-Frémillon and Genevieve Chevalier and Inaam A. Nakchbandi and Deane Mosher and Marc R. Block and Corinne Albig\`es-Rizo and Daniel Bouvard Osteoblast mineralization requires $ \beta $1 integrin/ICAP-$1$-dependent fibronectin deposition . . . . . . . . . 307 Jaewon Ko and Gilberto J. Soler-Llavina and Marc V. Fuccillo and Robert C. Malenka and Thomas C. Südhof Neuroligins\slash LRRTMs prevent activity- and Ca$^{2+}$ /calmodulin-dependent synapse elimination in cultured neurons . . . . 323 Basile Darbellay and Serge Arnaudeau and Charles R. Bader and Stephane Konig and Laurent Bernheim STIM1L is a new actin-binding splice variant involved in fast repetitive Ca$^{2+}$ release . . . . . . . . . . . 335 Deanna G. Wilson and Khanhky Phamluong and Li Li and Mei Sun and Tim C. Cao and Peter S. Liu and Zora Modrusan and Wendy N. Sandoval and Linda Rangell and Richard A. D. Carano and Andrew S. Peterson and Mark J. Solloway Global defects in collagen secretion in a Mia3/TANGO1 knockout mouse . . . . . . 347
Ben Short PARP retains Ku at double-strand breaks ?? Ben Short RNA targeting gets competitive . . . . . ?? Ben Short A COG in the retrograde transport machinery . . . . . . . . . . . . . . . ?? Ben Short Nuclear envelope starts with a clean sheet . . . . . . . . . . . . . . . . . 351 Caitlin Sedwick Ulrike Eggert: Big things from small molecules . . . . . . . . . . . . . . . 352 Pamela A. Harvey and Leslie A. Leinwand Cellular mechanisms of cardiomyopathy 355 C. Anne-Marie Couto and Hong-Yu Wang and Joanna C. A. Green and Rhian Kiely and Robert Siddaway and Christine Borer and Catherine J. Pears and Nicholas D. Lakin PARP regulates nonhomologous end joining through retention of Ku at double-strand breaks . . . . . . . . . . . . . . . . . 367 Eva M. Gusnowski and Martin Srayko Visualization of dynein-dependent microtubule gliding at the cell cortex: implications for spindle positioning . . 377 Thomas Becker and Lena-Sophie Wenz and Vivien Krüger and Waltraut Lehmann and Judith M. Müller and Luise Goroncy and Nicole Zufall and Trevor Lithgow and Bernard Guiard and Agnieszka Chacinska and Richard Wagner and Chris Meisinger and Nikolaus Pfanner The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins . . . . . . . . 387 Dra\vzen Papi\'c and Katrin Krumpe and Jovana Dukanovic and Kai S. Dimmer and Doron Rapaport Multispan mitochondrial outer membrane protein Ugo1 follows a unique Mim1-dependent import pathway . . . . . 397 Patima Sdek and Peng Zhao and Yaping Wang and Chang-jiang Huang and Christopher Y. Ko and Peter C. Butler and James N. Weiss and W. Robb MacLellan Rb and p130 control cell cycle gene silencing to maintain the postmitotic phenotype in cardiac myocytes . . . . . 407 Lei Lu and Mark S. Ladinsky and Tomas Kirchhausen Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly . . . . . 425 Ilham A. Muslimov and Mihir V. Patel and Arthur Rose and Henri Tiedge Spatial code recognition in neuronal RNA targeting: Role of RNA--hnRNP A2 interactions . . . . . . . . . . . . . . 441 Orly Laufman and WanJin Hong and Sima Lev The COG complex interacts directly with Syntaxin 6 and positively regulates endosome--to--TGN retrograde transport 459 Johannes Förtsch and Eric Hummel and Melanie Krist and Benedikt Westermann The myosin-related motor protein Myo2 is an essential mediator of bud-directed mitochondrial movement in yeast . . . . 473 Patricia L. Martínez-Morales and Ruth Diez del Corral and Isabel Olivera-Martínez and Alejandra C. Quiroga and Raman M. Das and Julio A. Barbas and Kate G. Storey and Aixa V. Morales FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk . . . . . . . . 489 Ilaria Prada and Julie Marchaland and Paola Podini and Lorenzo Magrassi and Rosalba D'Alessandro and Paola Bezzi and Jacopo Meldolesi REST\slash NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes . . . . . . . . . . . . . . . 505
Mitch Leslie RanBP2 stops importin-$ \beta $ from running away . . . . . . . . . . . . . . ?? Mitch Leslie Lis1 finds new digs on the desmosome . . ?? Mitch Leslie Switching adherens junctions to seal wounds . . . . . . . . . . . . . . . . . ?? Mitch Leslie Aurora B goes the distance . . . . . . . 509 Caitlin Sedwick Alejandro Sánchez Alvarado: Bootstrapping flatworms into the molecular age . . . . 510 Bill Wickstead and Keith Gull The evolution of the cytoskeleton . . . 513 Johanna S. Carroll and Sarah E. Munchel and Karsten Weis The DExD/H box ATPase Dhh1 functions in translational repression, mRNA decay, and processing body dynamics . . . . . . 527 Enxiu Wang and Edward R. Ballister and Michael A. Lampson Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient . . . . . . . . . . . . . . . . 539 Yazhong Tao and Ronald L. Neppl and Zhan-Peng Huang and Jianfu Chen and Ru-Hang Tang and Ru Cao and Yi Zhang and Suk-Won Jin and Da-Zhi Wang The histone methyltransferase Set7/9 promotes myoblast differentiation and myofibril assembly . . . . . . . . . . . 551 Raquel Domínguez-Kelly and Yusé Martín and Stephane Koundrioukoff and Marvin E. Tanenbaum and Veronique A. J. Smits and René H. Medema and Michelle Debatisse and Raimundo Freire Wee1 controls genomic stability during replication by regulating the Mus81-Eme1 endonuclease . . . . . . . . . . . . . . 567 Katharina Rzeczkowski and Knut Beuerlein and Helmut Müller and Oliver Dittrich-Breiholz and Heike Schneider and Daniela Kettner-Buhrow and Helmut Holtmann and Michael Kracht c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies 581 Masakazu Hamada and Anna Haeger and Karthik B. Jeganathan and Janine H. van Ree and Liviu Malureanu and Sarah Wälde and Jomon Joseph and Ralph H. Kehlenbach and Jan M. van Deursen Ran-dependent docking of importin-$ \beta $ to RanBP2/Nup358 filaments is essential for protein import and cell viability . . . . . . . . . . . . . . . 597 Thomas D. Nightingale and Ian J. White and Emily L. Doyle and Mark Turmaine and Kimberly J. Harrison-Lavoie and Kathleen F. Webb and Louise P. Cramer and Daniel F. Cutler Actomyosin II contractility expels von Willebrand factor from Weibel--Palade bodies during exocytosis . . . . . . . . 613 Kaelyn D. Sumigray and Hsin Chen and Terry Lechler Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis . . . . . . . 631 Katsutoshi Taguchi and Takashi Ishiuchi and Masatoshi Takeichi Mechanosensitive EPLIN-dependent remodeling of adherens junctions regulates epithelial reshaping . . . . . 643
Ben Short Vpr loosens chromatid ties . . . . . . . ?? Ben Short DLK makes neuronal cutbacks . . . . . . ?? Ben Short Arf1 doubles up to release vesicles . . ?? Ben Short Budding yeast star in their own biofilm 659 Caitlin Sedwick Ruth Lehmann: Germ cells do things differently . . . . . . . . . . . . . . 660 Elizabeth H. Williams and Tom Misteli New Tools for JCB . . . . . . . . . . . 663 Peter H. Thorpe and David Alvaro and Michael Lisby and Rodney Rothstein Bringing Rad52 foci into focus . . . . . 665 Reinhard Windoffer and Michael Beil and Thomas M. Magin and Rudolf E. Leube Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia . . . . . . . . . . . . . . . 669 Libu\vse Váchová and Vratislav \vS\vtoví\vcek and Otakar Hlavá\vcek and Oleksandr Chernyavskiy and Lud\vek \vSt\vepánek and Lucie Kubínová and Zdena Palková Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies . . . . . . . . . 679 Mark T. Boyd and Nikolina Vlatkovi\'c and Carlos P. Rubbi The nucleolus directly regulates p53 export and degradation . . . . . . . . . 689 Shaun E. Peterson and Yinyin Li and Brian T. Chait and Max E. Gottesman and Richard Baer and Jean Gautier Cdk1 uncouples CtIP-dependent resection and Rad51 filament formation during M-phase double-strand break repair . . . 705 Mari Shimura and Yusuke Toyoda and Kenta Iijima and Masanobu Kinomoto and Kenzo Tokunaga and Kinya Yoda and Mitsuhiro Yanagida and Tetsutaro Sata and Yukihito Ishizaka Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation 721 Jason D. Berndt and Atsushi Aoyagi and Peitzu Yang and Jamie N. Anastas and Lan Tang and Randall T. Moon Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/$ \beta $-catenin signaling . . . . 737 Arundhati Sengupta Ghosh and Bei Wang and Christine D. Pozniak and Mark Chen and Ryan J. Watts and Joseph W. Lewcock DLK induces developmental neuronal degeneration via selective regulation of proapoptotic JNK activity . . . . . . . 751 Rainer Beck and Simone Prinz and Petra Diestelkötter-Bachert and Simone Röhling and Frank Adolf and Kathrin Hoehner and Sonja Welsch and Paolo Ronchi and Britta Brügger and John A. G. Briggs and Felix Wieland Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission . . . . . . . . . . . 765 Frank C. Brown and Carmel H. Schindelhaim and Suzanne R. Pfeffer GCC185 plays independent roles in Golgi structure maintenance and AP-1-mediated vesicle tethering . . . . . . . . . . . 779 Jennifer L. Rohn and David Sims and Tao Liu and Marina Fedorova and Frieder Schöck and Joseph Dopie and Maria K. Vartiainen and Amy A. Kiger and Norbert Perrimon and Buzz Baum Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype . . . . . . . . . . . . . . . 789 Jonathan H. Schatz and Elisa Oricchio and Andrew L. Wolfe and Man Jiang and Irina Linkov and Jocelyn Maragulia and Weiji Shi and Zhigang Zhang and Rajasekhar K. Vinagolu and Nen C. Pagano and John A. Porco and Julie Teruya-Feldstein and Neal Rosen and Andrew D. Zelenetz and Jerry Pelletier and Hans-Guido Wendel Targeting cap-dependent translation blocks converging survival signals by AKT and PIM kinases in lymphoma . . . . ?? Shirly Becker-Herman and Almut Meyer-Bahlburg and Marc A. Schwartz and Shaun W. Jackson and Kelly L. Hudkins and Chaohong Liu and Blythe D. Sather and Socheath Khim and Denny Liggitt and Wenxia Song and Gregg J. Silverman and Charles E. Alpers and David J. Rawlings WASp-deficient B cells play a critical, cell-intrinsic role in triggering autoimmunity . . . . . . . . . . . . . . ?? Patrick Viatour and Ursula Ehmer and Louis A. Saddic and Craig Dorrell and Jesper B. Andersen and Chenwei Lin and Anne-Flore Zmoos and Pawel K. Mazur and Bethany E. Schaffer and Austin Ostermeier and Hannes Vogel and Karl G. Sylvester and Snorri S. Thorgeirsson and Markus Grompe and Julien Sage Notch signaling inhibits hepatocellular carcinoma following inactivation of the RB pathway . . . . . . . . . . . . . . . ??
Mitch Leslie p63 delegates during skin development ?? Mitch Leslie MCPH1 keeps chromosomes strung out . . . ?? Mitch Leslie A kinase makes a connection . . . . . . ?? Mitch Leslie Mnemonic microRNAs help make memories 809 Caitlin Sedwick Shinya Inoué: Lighting the way in microscopy . . . . . . . . . . . . . . . 810 Lunhua Liu and Carole A. Parent TOR kinase complexes and cell migration 815 Michael Y. Fessing and Andrei N. Mardaryev and Michal R. Gdula and Andrey A. Sharov and Tatyana Y. Sharova and Valentina Rapisarda and Konstantin B. Gordon and Anna D. Smorodchenko and Krzysztof Poterlowicz and Giustina Ferone and Yoshinori Kohwi and Caterina Missero and Terumi Kohwi-Shigematsu and Vladimir A. Botchkarev p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis . . 825 Daisuke Yamashita and Keishi Shintomi and Takao Ono and Ioannis Gavvovidis and Detlev Schindler and Heidemarie Neitzel and Marc Trimborn and Tatsuya Hirano MCPH1 regulates chromosome condensation and shaping as a composite modulator of condensin II . . . . . . . . . . . . . . 841 Ben Moree and Corey B. Meyer and Colin J. Fuller and Aaron F. Straight CENP-C recruits M18BP1 to centromeres to promote CENP--A chromatin assembly . . . 855 Elina Shteyn and Lucy Pigati and Heike Fölsch Arf6 regulates AP-1B-dependent sorting in polarized epithelial cells . . . . . 873 Giordano Lippi and Joern R. Steinert and Emma L. Marczylo and Sabina D'Oro and Roberto Fiore and Ian D. Forsythe and Gerhard Schratt and Michele Zoli and Pierluigi Nicotera and Kenneth W. Young Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology . . . . . . . . . . . . 889 Yuejun Chen and Feifei Wang and Hui Long and Ying Chen and Ziyan Wu and Lan Ma GRK5 promotes F-actin bundling and targets bundles to membrane structures to control neuronal morphogenesis . . . 905 Ling Cheng and Cody Locke and Graeme W. Davis S6 kinase localizes to the presynaptic active zone and functions with PDK1 to control synapse development . . . . . . 921
Mitch Leslie Fibers keep cilia regular . . . . . . . ?? Mitch Leslie TPX2 is a drag on Eg5 . . . . . . . . . ?? Mitch Leslie Mobile moesin helps mitotic cells stretch out . . . . . . . . . . . . . . ?? Mitch Leslie Why a protein switches sides during translation . . . . . . . . . . . . . . 3 Ben Short Howard Chang: Gene regulation in time and space . . . . . . . . . . . . . . . 4 Cat M. Haglund and Matthew D. Welch Pathogens and polymers: Microbe--host interactions illuminate the cytoskeleton 7 Michael E. Werner and Peter Hwang and Fawn Huisman and Peter Taborek and Clare C. Yu and Brian J. Mitchell Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells . . . . 19 Kensuke Ninomiya and Naoyuki Kataoka and Masatoshi Hagiwara Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor . . . . . . . . . . . . 27 Pen-Jen Lin and Candice G. Jongsma and Shuren Liao and Arthur E. Johnson Transmembrane segments of nascent polytopic membrane proteins control cytosol/ER targeting during membrane integration . . . . . . . . . . . . . . 41 Pen-Jen Lin and Candice G. Jongsma and Martin R. Pool and Arthur E. Johnson Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon . . . 55 Carly Willenborg and Jian Jing and Christine Wu and Hugo Matern and Jerome Schaack and Jemima Burden and Rytis Prekeris Interaction between FIP5 and SNX18 regulates epithelial lumen formation . . 71 Nan Ma and Janel Titus and Alyssa Gable and Jennifer L. Ross and Patricia Wadsworth TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle . . . . . . . . . . . . . . . . 87 Chantal Roubinet and Barbara Decelle and Gaëtan Chicanne and Jonas F. Dorn and Bernard Payrastre and François Payre and Sébastien Carreno Molecular networks linked by Moesin drive remodeling of the cell cortex during mitosis . . . . . . . . . . . . . 99 Yi-Ru Pan and Chien-Lin Chen and Hong-Chen Chen FAK is required for the assembly of podosome rosettes . . . . . . . . . . . 113 Carla Danussi and Alessandra Petrucco and Bruna Wassermann and Eliana Pivetta and Teresa Maria Elisa Modica and Lisa Del Bel Belluz and Alfonso Colombatti and Paola Spessotto EMILIN1-$ \alpha $4\slash $ \alpha $9 integrin interaction inhibits dermal fibroblast and keratinocyte proliferation . . . . . . . . . . . . . 131 Michelle J. Doyle and Sheng Zhou and Kathleen Kelly Tanaka and Addolorata Pisconti and Nicholas H. Farina and Brian P. Sorrentino and Bradley B. Olwin Abcg2 labels multiple cell types in skeletal muscle and participates in muscle regeneration . . . . . . . . . . 147
Ben Short Good housekeeping maintains a healthy liver . . . . . . . . . . . . . . . . . ?? Ben Short Twins activate! Form of a new centriole! ?? Ben Short Schwann cells cover their territory . . ?? Ben Short Locating a mitochondrial scaffold on the map . . . . . . . . . . . . . . . . . . 167 Caitlin Sedwick Anna Akhmanova: Great tips on microtubules . . . . . . . . . . . . . . 168 Katherine L. Wilson and Scott C. Dawson Functional evolution of nuclear structure . . . . . . . . . . . . . . . 171 Philipp Stelter and Ruth Kunze and Jessica Fischer and Ed Hurt Probing the nucleoporin FG repeat network defines structural and functional features of the nuclear pore complex . . . . . . . . . . . . . . . . 183 Julie Y. Yi and Kassandra M. Ori-McKenney and Richard J. McKenney and Michael Vershinin and Steven P. Gross and Richard B. Vallee High-resolution imaging reveals indirect coordination of opposite motors and a role for LIS1 in high-load axonal transport . . . . . . . . . . . . . . . 193 Rachel Kama and Vydehi Kanneganti and Christian Ungermann and Jeffrey E. Gerst The yeast Batten disease orthologue Btn1 controls endosome-Golgi retrograde transport via SNARE assembly . . . . . . 203 Natasha T. Snider and Sujith V. W. Weerasinghe and Amika Singla and Jessica M. Leonard and Shinichiro Hanada and Philip C. Andrews and Anna S. Lok and M. Bishr Omary Energy determinants GAPDH and NDPK act as genetic modifiers for hepatocyte inclusion formation . . . . . . . . . . 217 Christopher W. Brownlee and Joey E. Klebba and Daniel W. Buster and Gregory C. Rogers The Protein Phosphatase 2A regulatory subunit Twins stabilizes Plk4 to induce centriole amplification . . . . . . . . 231 Hiroyuki Nakajima and Takuji Tanoue Lulu2 regulates the circumferential actomyosin tensile system in epithelial cells through p114RhoGEF . . . . . . . . 245 Ying-bei Chen and Miguel A. Aon and Yi-Te Hsu and Lucian Soane and Xinchen Teng and J. Michael McCaffery and Wen-Chih Cheng and Bing Qi and Hongmei Li and Kambiz N. Alavian and Margaret Dayhoff-Brannigan and Shifa Zou and Fernando J. Pineda and Brian O'Rourke and Young H. Ko and Peter L. Pedersen and Leonard K. Kaczmarek and Elizabeth A. Jonas and J. Marie Hardwick Bcl-x$_L$ regulates mitochondrial energetics by stabilizing the inner membrane potential . . . . . . . . . . . 263 Toshiyuki Bohgaki and Julien Mozo and Leonardo Salmena and Elzbieta Matysiak-Zablocki and Miyuki Bohgaki and Otto Sanchez and Andreas Strasser and Anne Hakem and Razqallah Hakem Caspase-8 inactivation in T cells increases necroptosis and suppresses autoimmunity in Bim$^{-/} - $ mice . . . 277 Monika S. Brill and Jeff W. Lichtman and Wesley Thompson and Yi Zuo and Thomas Misgeld Spatial constraints dictate glial territories at murine neuromuscular junctions . . . . . . . . . . . . . . . 293 Eusebio Perdiguero and Pedro Sousa-Victor and Vanessa Ruiz-Bonilla and Merc\`e Jardí and Carme Caelles and Antonio L. Serrano and Pura Muñoz-Cánoves p38/MKP-$1$-regulated AKT coordinates macrophage transitions and resolution of inflammation during tissue repair . . . 307 Suzanne Hoppins and Sean R. Collins and Ann Cassidy-Stone and Eric Hummel and Rachel M. DeVay and Laura L. Lackner and Benedikt Westermann and Maya Schuldiner and Jonathan S. Weissman and Jodi Nunnari A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria . . . . . . 323 Zita Carvalho-Santos and Juliette Azimzadeh and José. B. Pereira-Leal and Mónica Bettencourt-Dias Tracing the origins of centrioles, cilia, and flagella . . . . . . . . . . 341
Ben Short Ran drags spindles through the Mud . . . ?? Ben Short PKA cuts down on HDAC4 signaling . . . . ?? Ben Short PLD gives nutritional advice to mTORC1 ?? Ben Short Imaging $ \beta $ amyloid's pore performance . . . . . . . . . . . . . . 345 Caitlin Sedwick Benny Geiger: a force in the study of focal adhesions . . . . . . . . . . . . 346 Matteo Bonazzi and Pascale Cossart Impenetrable barriers or entry portals? The role of cell--cell adhesion during infection . . . . . . . . . . . . . . . 349 Eric F. Joyce and Michael Pedersen and Stanley Tiong and Sanese K. White-Brown and Anshu Paul and Shelagh D. Campbell and Kim S. McKim Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repair . . . . . . . . . 359 Brett Wee and Christopher A. Johnston and Kenneth E. Prehoda and Chris Q. Doe Canoe binds RanGTP to promote Pins$^{\rm TPR}$/Mud-mediated spindle orientation 369 Hyun Sub Hwang and Sang Gil Hwang and Jun-Ho Cho and Ji Soo Chae and Kyoung Wan Yoon and Ssang-Goo Cho and Eui-Ju Choi CIIA functions as a molecular switch for the Rac1-specific GEF activity of SOS1 377 Seiji Matsumoto and Motoshi Hayano and Yutaka Kanoh and Hisao Masai Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation . . 387 Johannes Backs and Barbara C. Worst and Lorenz H. Lehmann and David M. Patrick and Zegeye Jebessa and Michael M. Kreusser and Qiang Sun and Lan Chen and Claudia Heft and Hugo A. Katus and Eric N. Olson Selective repression of MEF2 activity by PKA-dependent proteolysis of HDAC4 . . . 403 Nam Hee Kim and Hyun Sil Kim and Xiao-Yan Li and Inhan Lee and Hyung-Seok Choi and Shi Eun Kang and So Young Cha and Joo Kyung Ryu and Dojun Yoon and Eric R. Fearon and R. Grant Rowe and Sanghyuk Lee and Christopher A. Maher and Stephen J. Weiss and Jong In Yook A p53/miRNA-34 axis regulates Snail1-dependent cancer cell epithelial--mesenchymal transition . . . 417 Mee-Sup Yoon and Guangwei Du and Jonathan M. Backer and Michael A. Frohman and Jie Chen Class III PI-3-kinase activates phospholipase D in an amino acid--sensing mTORC1 pathway . . . . . . 435 Eleni Petsalaki and Tonia Akoumianaki and Elizabeth J. Black and David A. F. Gillespie and George Zachos Phosphorylation at serine 331 is required for Aurora B activation . . . . 449 Tiina Tamm and Agnes Grallert and Emily P. S. Grossman and Isabel Alvarez-Tabares and Frances E. Stevens and Iain M. Hagan Brr6 drives the \bionameSchizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle . . . . . . . 467 Qian Chen and Thomas D. Pollard Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring . . . . . . . . . . . . 485 Pengbo Wang and Christoph Ballestrem and Charles H. Streuli The C terminus of talin links integrins to cell cycle progression . . . . . . . 499 Angelo Demuro and Martin Smith and Ian Parker Single-channel Ca$^{2+}$ imaging implicates A$ \beta $1-42 amyloid pores in Alzheimer's disease pathology . . . . 515 Matteo Bonazzi and Lavanya Vasudevan and Adeline Mallet and Martin Sachse and Anna Sartori and Marie-Christine Prevost and Allison Roberts and Sabrina B. Taner and Jeremy D. Wilbur and Frances M. Brodsky and Pascale Cossart Clathrin phosphorylation is required for actin recruitment at sites of bacterial adhesion and internalization . . . . . . 525
Ben Short Bridging the gap between atlastin conformations . . . . . . . . . . . . . ?? Ben Short For Myo4p, two heads are better than one ?? Ben Short A new spin on radial spokes . . . . . . ?? Ben Short Setting a new standard for kinetochores 539 Caitlin Sedwick Denise Montell: Lighting the way in border cell migration . . . . . . . . . 540 Stephanie L. Maiden and Jeff Hardin The secret life of $ \alpha $-catenin: Moonlighting in morphogenesis . . . . . 543 Ana Carmena and Aljona Makarova and Stephan Speicher The Rap1--Rgl--Ral signaling network regulates neuroblast cortical polarity and spindle orientation . . . . . . . . 553 Valerie C. Coffman and Pengcheng Wu and Mark R. Parthun and Jian-Qiu Wu CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast . . . . . . . 563 Josh Lawrimore and Kerry S. Bloom and E. D. Salmon Point centromeres contain more than a single centromere-specific Cse4 (CENP--A) nucleosome . . . . . . . . . . 573 Jun-Song Chen and Lucy X. Lu and Melanie D. Ohi and Kevin M. Creamer and Chauca English and Janet F. Partridge and Ryoma Ohi and Kathleen L. Gould Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation . . . . . . . . . 583 Zuni I. Bassi and Koen J. Verbrugghe and Luisa Capalbo and Stephen Gregory and Emilie Montembault and David M. Glover and Pier Paolo D'Avino Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis . . . . . . . . . . . 595 Jeanne Morin-Leisk and Simran G. Saini and Xin Meng and Alexander M. Makhov and Peijun Zhang and Tina H. Lee An intramolecular salt bridge drives the soluble domain of GTP-bound atlastin into the postfusion conformation . . . . 605 Sebastian Specht and Stephanie B. M. Miller and Axel Mogk and Bernd Bukau Hsp42 is required for sequestration of protein aggregates into deposition sites in \bionameSaccharomyces cerevisiae . . 617 Elena B. Krementsova and Alex R. Hodges and Carol S. Bookwalter and Thomas E. Sladewski and Mirko Travaglia and H. Lee Sweeney and Kathleen M. Trybus Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex . . . . . . . . . . . 631 Christian Schulz and Oleksandr Lytovchenko and Jonathan Melin and Agnieszka Chacinska and Bernard Guiard and Piotr Neumann and Ralf Ficner and Olaf Jahn and Bernhard Schmidt and Peter Rehling Tim50's presequence receptor domain is essential for signal driven transport across the TIM23 complex . . . . . . . . 643 Derek C. Prosser and Theodore G. Drivas and Lymarie Maldonado-Báez and Beverly Wendland Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin 657 Gaia Pigino and Khanh Huy Bui and Aditi Maheshwari and Pietro Lupetti and Dennis Diener and Takashi Ishikawa Cryoelectron tomography of radial spokes in cilia and flagella . . . . . . . . . 673 Toshihide Kurihara and Peter D. Westenskow and Tim U. Krohne and Edith Aguilar and Randall S. Johnson and Martin Friedlander Astrocyte pVHL and HIF-$ \alpha $ isoforms are required for embryonic-to-adult vascular transition in the eye . . . . . . . . . . . . . . . 689
Mitch Leslie VPS35 leaves endosomes lost in transition . . . . . . . . . . . . . . . ?? Mitch Leslie The actomyosin ring bulks up . . . . . . ?? Mitch Leslie HPK1 tones down the T cell receptor . . ?? Mitch Leslie How eisosomes help the plasma membrane get organized . . . . . . . . . . . . . 705 Caitlin Sedwick Vann Bennett: How ankyrin holds it all together . . . . . . . . . . . . . . . . 706 Eric M. Pietras and Matthew R. Warr and Emmanuelle Passegué Cell cycle regulation in hematopoietic stem cells . . . . . . . . . . . . . . . 709 Kimihide Hayakawa and Hitoshi Tatsumi and Masahiro Sokabe Actin filaments function as a tension sensor by tension-dependent binding of cofilin to the filament . . . . . . . . 721 Xiao-Yan Li and Xiaoming Zhou and R. Grant Rowe and Yuexian Hu and David D. Schlaepfer and Dusko Ili\'c and Gregory Dressler and Ann Park and Jun-Lin Guan and Stephen J. Weiss Snail1 controls epithelial--mesenchymal lineage commitment in focal adhesion kinase--null embryonic cells . . . . . . 729 Marie Regairaz and Yong-Wei Zhang and Haiqing Fu and Keli K. Agama and Nalini Tata and Surbhi Agrawal and Mirit I. Aladjem and Yves Pommier Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase I--DNA complexes . . . . . 739 Carla P. Guimaraes and Jan E. Carette and Malini Varadarajan and John Antos and Maximilian W. Popp and Eric Spooner and Thijn R. Brummelkamp and Hidde L. Ploegh Identification of host cell factors required for intoxication through use of modified cholera toxin . . . . . . . . . 751 Lei Wen and Fu-Lei Tang and Yan Hong and Shi-Wen Luo and Chun-Lei Wang and Wanxia He and Chengyong Shen and Ji-Ung Jung and Fei Xiong and Dae-hoon Lee and Quan-Guang Zhang and Darrell Brann and Tae-Wan Kim and Riqiang Yan and Lin Mei and Wen-Cheng Xiong VPS35 haploinsufficiency increases Alzheimer's disease neuropathology . . . 765 Deborah Zyss and Hani Ebrahimi and Fanni Gergely Casein kinase I delta controls centrosome positioning during T cell activation . . . . . . . . . . . . . . . 781 Meredith E. K. Calvert and Graham D. Wright and Fong Yew Leong and Keng-Hwee Chiam and Yinxiao Chen and Gregory Jedd and Mohan K. Balasubramanian Myosin concentration underlies cell size-dependent scalability of actomyosin ring constriction . . . . . . . . . . . 799 Moshe S. Kim and Carol D. Froese and Mathew P. Estey and William S. Trimble SEPT9 occupies the terminal positions in septin octamers and mediates polymerization-dependent functions in abscission . . . . . . . . . . . . . . . 815 Shirin Meher Pocha and Anna Shevchenko and Elisabeth Knust Crumbs regulates rhodopsin transport by interacting with and stabilizing myosin V . . . . . . . . . . . . . . . . . . . 827 Rémi Lasserre and Céline Cuche and Ronnie Blecher-Gonen and Evgeny Libman and Elise Biquand and Anne Danckaert and Deborah Yablonski and Andrés Alcover and Vincenzo Di Bartolo Release of serine/threonine-phosphorylated adaptors from signaling microclusters down-regulates T cell activation . . . . 839 Sébastien Nola and Reiko Daigaku and Kasia Smolarczyk and Maryke Carstens and Belen Martin-Martin and Gregory Longmore and Maryse Bailly and Vania M. M. Braga Ajuba is required for Rac activation and maintenance of E-cadherin adhesion . . . 855 Beate K. Straub and Steffen Rickelt and Ralf Zimbelmann and Christine Grund and Caecilia Kuhn and Marcus Iken and Michael Ott and Peter Schirmacher and Werner W. Franke E--N-cadherin heterodimers define novel adherens junctions connecting endoderm-derived cells . . . . . . . . . 873 Lena Karotki and Juha T. Huiskonen and Christopher J. Stefan and Natasza E. Zió\lkowska and Robyn Roth and Michal A. Surma and Nevan J. Krogan and Scott D. Emr and John Heuser and Kay Grünewald and Tobias C. Walther Eisosome proteins assemble into a membrane scaffold . . . . . . . . . . . 889 Marco A. O. Magalhaes and Daniel R. Larson and Christopher C. Mader and Jose Javier Bravo-Cordero and Hava Gil-Henn and Matthew Oser and Xiaoming Chen and Anthony J. Koleske and John Condeelis Cortactin phosphorylation regulates cell invasion through a pH-dependent pathway 903
Ben Short Lipid droplets fatten up with Fsp27 . . ?? Ben Short CUPS provide a handle on Acb1 secretion ?? Ben Short Cell death helps give closure . . . . . ?? Ben Short Shs1 rounds out the septin repertoire 923 Caitlin Sedwick Michael Overholtzer: Answering existential questions on entosis . . . . 924 Tim P. Levine Lipid traffic: Osh4p makes an unexpected exchange . . . . . . . . . . . . . . . . 927 Hiroshi Ashida and Hitomi Mimuro and Michinaga Ogawa and Taira Kobayashi and Takahito Sanada and Minsoo Kim and Chihiro Sasakawa Cell death and infection: a double-edged sword for host and pathogen survival . . 931 Eric Alix and Shaeri Mukherjee and Craig R. Roy Subversion of membrane transport pathways by vacuolar pathogens . . . . . 943 Jingyi Gong and Zhiqi Sun and Lizhen Wu and Wenyi Xu and Nicole Schieber and Dijin Xu and Guanghou Shui and Hongyuan Yang and Robert G. Parton and Peng Li Fsp27 promotes lipid droplet growth by lipid exchange and transfer at lipid droplet contact sites . . . . . . . . . 953 Maud de Saint-Jean and Vanessa Delfosse and Dominique Douguet and Gaëtan Chicanne and Bernard Payrastre and William Bourguet and Bruno Antonny and Guillaume Drin Osh4p exchanges sterols for phosphatidylinositol $4$-phosphate between lipid bilayers . . . . . . . . . 965 Caroline Bruns and J. Michael McCaffery and Amy J. Curwin and Juan M. Duran and Vivek Malhotra Biogenesis of a novel compartment for autophagosome-mediated unconventional protein secretion . . . . . . . . . . . 979 Galo Garcia and Aurelie Bertin and Zhu Li and Yi Song and Michael A. McMurray and Jeremy Thorner and Eva Nogales Subunit-dependent modulation of septin assembly: Budding yeast septin Shs1 promotes ring and gauze formation . . . 993 Kazuya Hori and Anindya Sen and Tom Kirchhausen and Spyros Artavanis-Tsakonas Synergy between the ESCRT--III complex and Deltex defines a ligand-independent Notch signal . . . . . . . . . . . . . . 1005 Aikaterini Daskalaki and Nevine A. Shalaby and Kristina Kux and Giorgos Tsoumpekos and George D. Tsibidis and Marc A. T. Muskavitch and Christos Delidakis Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized 1017 Peter W. Janes and Bettina Griesshaber and Lakmali Atapattu and Eva Nievergall and Linda L. Hii and Anneloes Mensinga and Chanly Chheang and Bryan W. Day and Andrew W. Boyd and Philippe I. Bastiaens and Claus Jòrgensen and Tony Pawson and Martin Lackmann Eph receptor function is modulated by heterooligomerization of A and B type Eph receptors . . . . . . . . . . . . . 1033 Yoshifumi Yamaguchi and Naomi Shinotsuka and Keiko Nonomura and Kiwamu Takemoto and Keisuke Kuida and Hiroki Yosida and Masayuki Miura Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure . . . . . . . . . . 1047 Christopher L. Brett and Rachael L. Plemel and Braden T. Lobingier and Marissa Vignali and Stanley Fields and Alexey J. Merz Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase . . 1061 Lukas Kenner and Astrid Hoebertz and F. Timo Beil and Niamh Keon and Florian Karreth and Robert Eferl and Harald Scheuch and Agnieszka Szremska and Michael Amling and Marina Schorpp-Kistner and Peter Angel and Erwin F. Wagner Mice lacking JunB are osteopenic due to cell-autonomous osteoblast and osteoclast defects . . . . . . . . . . . 1063
Mitch Leslie Shining new light on the function of Aurora A . . . . . . . . . . . . . . . . ?? Mitch Leslie Protocadherins bring cells together . . ?? Mitch Leslie Cdc6 licenses tumor growth . . . . . . . ?? Mitch Leslie Neuregulins show their support for neuromuscular junctions . . . . . . . . 1067 Caitlin Sedwick Rong Li: Tipping the balance in cell biology . . . . . . . . . . . . . . . . 1068 Joe Grove and Mark Marsh The cell biology of receptor-mediated virus entry . . . . . . . . . . . . . . 1071 Anne Marie Krachler and Andrew R. Woolery and Kim Orth Manipulation of kinase signaling by bacterial pathogens . . . . . . . . . . 1083 Kwanwoo Lee and Kunsoo Rhee PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis . . . . . . . . . . . . 1093 Nadia Hégarat and Ewan Smith and Gowri Nayak and Shunichi Takeda and Patrick A. Eyers and Helfrid Hochegger Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics . . . . . . . . . . 1103 Michelle R. Emond and Sayantanee Biswas and Cheasequah J. Blevins and James D. Jontes A complex of Protocadherin-19 and N-cadherin mediates a novel mechanism of cell adhesion . . . . . . . . . . . . . 1115 Maria Sideridou and Roubini Zakopoulou and Konstantinos Evangelou and Michalis Liontos and Athanassios Kotsinas and Emmanouil Rampakakis and Sarantis Gagos and Kaoru Kahata and Kristina Grabusic and Kalliopi Gkouskou and Ioannis P. Trougakos and Evangelos Kolettas and Alexandros G. Georgakilas and Sinisa Volarevic and Aristides G. Eliopoulos and Maria Zannis-Hadjopoulos and Aristidis Moustakas and Vassilis G. Gorgoulis Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins . . . . . . . . . . 1123 María Verónica Baez and Luciana Luchelli and Darío Maschi and Martín Habif and Malena Pascual and María Gabriela Thomas and Graciela Lidia Boccaccio Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation . . 1141 Zhao Wang and Huisheng Liu and Yiwen Gu and Edwin R. Chapman Reconstituted synaptotagmin I mediates vesicle docking, priming, and fusion . . 1159 Nadine Schmidt and Mohammed Akaaboune and Nadesan Gajendran and Isabel Martinez-Peña y Valenzuela and Sarah Wakefield and Raphael Thurnheer and Hans Rudolf Brenner Neuregulin\slash ErbB regulate neuromuscular junction development by phosphorylation of $ \alpha $-dystrobrevin . . . . . . . . . . . . . 1171 Oxana E. Nekrasova and Evangeline V. Amargo and William O. Smith and Jing Chen and Geri E. Kreitzer and Kathleen J. Green Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes 1185 Matteo Bonazzi and Pascale Cossart Impenetrable barriers or entry portals? The role of cell--cell adhesion during infection . . . . . . . . . . . . . . . 1205
Ben Short Ubiquitylation keeps the nucleus moving ?? Ben Short How meiotic chromosomes meet their match ?? Ben Short A firmer understanding of muscle fibrosis . . . . . . . . . . . . . . . . ?? Ben Short Building up actin at adherens junctions 3 Caitlin Sedwick Keith Burridge: Cultivating knowledge on Rho . . . . . . . . . . . . . . . . . . 4 Jack T. Wang and Zachary A. Medress and Ben A. Barres Axon degeneration: Molecular mechanisms of a self-destruction pathway . . . . . 7 Akira Hayakawa and Anna Babour and Lucie Sengmanivong and Catherine Dargemont Ubiquitylation of the nuclear pore complex controls nuclear migration during mitosis in S. cerevisiae . . . . 19 Jason Howitt and Jenny Lackovic and Ley-Hian Low and Adam Naguib and Alison Macintyre and Choo-Peng Goh and Jennifer K. Callaway and Vicki Hammond and Tim Thomas and Matthew Dixon and Ulrich Putz and John Silke and Perry Bartlett and Baoli Yang and Sharad Kumar and Lloyd C. Trotman and Seong-Seng Tan Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia . . . . . . 29 Kathleen Zylbersztejn and Maja Petkovic and Andrea Burgo and Marie Deck and Sonia Garel and Séverine Marcos and Evelyne Bloch-Gallego and Fatiha Nothias and Guido Serini and Dominique Bagnard and Thomas Binz and Thierry Galli The vesicular SNARE Synaptobrevin is required for Semaphorin 3A axonal repulsion . . . . . . . . . . . . . . . 37 David J. Wynne and Ofer Rog and Peter M. Carlton and Abby F. Dernburg Dynein-dependent processive chromosome motions promote homologous pairing in \bionameC. elegans meiosis . . . . . . . 47 Nikolaos Giagtzoglou and Shinya Yamamoto and Diana Zitserman and Hillary K. Graves and Karen L. Schulze and Hao Wang and Hayley Klein and Fabrice Roegiers and Hugo J. Bellen dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions . . 65 Donglei Zhang and Nora R. Isack and Doreen R. Glodowski and Jie Liu and Carlos Chih-Hsiung Chen and X. Z. Shawn Xu and Barth D. Grant and Christopher Rongo RAB-6.2 and the retromer regulate glutamate receptor recycling through a retrograde pathway . . . . . . . . . . . 85 Julia K. Gilden and Sebastian Peck and Yi-Chun M. Chen and Matthew F. Krummel The septin cytoskeleton facilitates membrane retraction during motility and blebbing . . . . . . . . . . . . . . . . 103 Vivian W. Tang and William M. Brieher $ \alpha $-Actinin-4\slash FSGS1 is required for Arp2/$3$-dependent actin assembly at the adherens junction . . . 115 Yamei Yu and Jianghai Zhu and Li-Zhi Mi and Thomas Walz and Hao Sun and JianFeng Chen and Timothy A. Springer Structural specializations of $ \alpha_4 \beta_7 $, an integrin that mediates rolling adhesion . . . . . . . . . . . . 131 Nikola Arsic and Nawal Bendris and Marion Peter and Christina Begon-Pescia and Cosette Rebouissou and Gilles Gadéa and Nathalie Bouquier and Frédéric Bibeau and Bénédicte Lemmers and Jean Marie Blanchard A novel function for Cyclin A2: Control of cell invasion via RhoA signaling . . 147 Esther Ardite and Eusebio Perdiguero and Berta Vidal and Susana Gutarra and Antonio L. Serrano and Pura Muñoz-Cánoves PAI-$1$-regulated miR-21 defines a novel age-associated fibrogenic pathway in muscular dystrophy . . . . . . . . . . . 163 Carla Danussi and Alessandra Petrucco and Bruna Wassermann and Eliana Pivetta and Teresa Maria Elisa Modica and Lisa Del Bel Belluz and Alfonso Colombatti and Paola Spessotto EMILIN1-$ \alpha $4\slash $ \alpha $9 integrin interaction inhibits dermal fibroblast and keratinocyte proliferation . . . . . . . . . . . . . 177
Leslie Mitch Licensing factors lose their credentials ?? Leslie Mitch n-Syb makes a dangerous delivery . . . . ?? Leslie Mitch DGK-$ \alpha $ keeps integrins close to the edge . . . . . . . . . . . . . . . . ?? Mitch Leslie Spare the Rod1, spoil transporter endocytosis . . . . . . . . . . . . . . 181 Caitlin Sedwick Tobias Walther: Floating ideas on lipids 182 Bryan Serrels and Margaret C. Frame FAK and talin: Who is taking whom to the integrin engagement party? . . . . . . . 185 Ana Maria Rojas and Gloria Fuentes and Antonio Rausell and Alfonso Valencia The Ras protein superfamily: Evolutionary tree and role of conserved amino acids . . . . . . . . . . . . . . 189 Xiao Zhou and Katja Graumann and David E. Evans and Iris Meier Novel plant SUN--KASH bridges are involved in RanGAP anchoring and nuclear shape determination . . . . . . . . . . 203 Mathias J. Gerl and Julio L. Sampaio and Severino Urban and Lucie Kalvodova and Jean-Marc Verbavatz and Beth Binnington and Dirk Lindemann and Clifford A. Lingwood and Andrej Shevchenko and Cornelia Schroeder and Kai Simons Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane . . . . . . . . . . 213 Christine Lawson and Ssang-Taek Lim and Sean Uryu and Xiao Lei Chen and David A. Calderwood and David D. Schlaepfer FAK promotes recruitment of talin to nascent adhesions to control cell motility . . . . . . . . . . . . . . . . 223 Remi Sonneville and Matthieu Querenet and Ashley Craig and Anton Gartner and J. Julian Blow The dynamics of replication licensing in live \bionameCaenorhabditis elegans embryos . . . . . . . . . . . . . . . . 233 Michel Becuwe and Neide Vieira and David Lara and Jéssica Gomes-Rezende and Carina Soares-Cunha and Margarida Casal and Rosine Haguenauer-Tsapis and Olivier Vincent and Sandra Paiva and Sébastien Léon A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis . . . . . . . . 247 Adam Haberman and W. Ryan Williamson and Daniel Epstein and Dong Wang and Srisha Rina and Ian A. Meinertzhagen and P. Robin Hiesinger The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration . . . . . . . . . . . 261 Elena Rainero and Patrick T. Caswell and Patricia A. J. Muller and Joan Grindlay and Mary W. McCaffrey and Qifeng Zhang and Michael J. O. Wakelam and Karen H. Vousden and Andrea Graziani and Jim C. Norman Diacylglycerol kinase $ \alpha $ controls RCP-dependent integrin trafficking to promote invasive migration . . . . . . . . . . . . . . . 277 Oxana E. Nekrasova and Evangeline V. Amargo and William O. Smith and Jing Chen and Geri E. Kreitzer and Kathleen J. Green Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes 297
Ben Short A pyrimidine scheme to restore mRNA export . . . . . . . . . . . . . . . . . ?? Ben Short Myelinated axons need a 4.1G connection ?? Ben Short Focal adhesions degrade the ECM . . . . ?? Ben Short Stress fibers guide focal adhesions to maturity . . . . . . . . . . . . . . . . 301 Caitlin Sedwick Pamela Silver: Synthesizing a new biology . . . . . . . . . . . . . . . . 302 Jimmy Ouellet and Yves Barral Organelle segregation during mitosis: Lessons from asymmetrically dividing cells . . . . . . . . . . . . . . . . . 305 Liang Zhang and Priyabrata Das and Mirco Schmolke and Balaji Manicassamy and Yaming Wang and Xiaoyi Deng and Ling Cai and Benjamin P. Tu and Christian V. Forst and Michael G. Roth and David E. Levy and Adolfo García-Sastre and Jef de Brabander and Margaret A. Phillips and Beatriz M. A. Fontoura Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export . . . . . . 315 Chantal Christis and Sean Munro The small G protein Arl1 directs the trans-Golgi-specific targeting of the Arf1 exchange factors BIG1 and BIG2 . . 327 Aleksandra Ivanovic and Ido Horresh and Neev Golan and Ivo Spiegel and Helena Sabanay and Shahar Frechter and Shinichi Ohno and Nobuo Terada and Wiebke Möbius and Jack Rosenbluth and Nils Brose and Elior Peles The cytoskeletal adapter protein 4.1G organizes the internodes in peripheral myelinated nerves . . . . . . . . . . . 337 Inna V. Nechipurenko and Heather T. Broihier FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption . . . . . . . . . 345 Patrick W. Oakes and Yvonne Beckham and Jonathan Stricker and Margaret L. Gardel Tension is required but not sufficient for focal adhesion maturation without a stress fiber template . . . . . . . . . 363 Yu Wang and Mark A. McNiven Invasive matrix degradation at focal adhesions occurs via protease recruitment by a FAK--p130Cas complex 375 Christine Lawson and Ssang-Taek Lim and Sean Uryu and Xiao Lei Chen and David A. Calderwood and David D. Schlaepfer FAK promotes recruitment of talin to nascent adhesions to control cell motility . . . . . . . . . . . . . . . . 387
Mitch Leslie No crystal structure? No problem . . . . ?? Mitch Leslie Slow but steady for NF-$ \kappa $B . . . ?? Mitch Leslie Caspase conspiracy sets up cells for death . . . . . . . . . . . . . . . . . ?? Mitch Leslie Knl1 shows another face . . . . . . . . 391 Caitlin Sedwick Sergio Grinstein: Phagocytosis step by step . . . . . . . . . . . . . . . . . . 392 Pengfei Lu and Valerie M. Weaver and Zena Werb The extracellular matrix: a dynamic niche in cancer progression . . . . . . 395 Sandra Maday and Karen E. Wallace and Erika L. F. Holzbaur Autophagosomes initiate distally and mature during transport toward the cell soma in primary neurons . . . . . . . . 407 Javier Fernandez-Martinez and Jeremy Phillips and Matthew D. Sekedat and Ruben Diaz-Avalos and Javier Velazquez-Muriel and Josef D. Franke and Rosemary Williams and David L. Stokes and Brian T. Chait and Andrej Sali and Michael P. Rout Structure--function mapping of a heptameric module in the nuclear pore complex . . . . . . . . . . . . . . . . 419 Emanuele Roscioli and Laura Di Francesco and Alessio Bolognesi and Maria Giubettini and Serena Orlando and Amnon Harel and Maria Eugenia Schinin\`a and Patrizia Lavia Importin-$ \beta $ negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores . . 435 Veronica Krenn and Annemarie Wehenkel and Xiaozheng Li and Stefano Santaguida and Andrea Musacchio Structural analysis reveals features of the spindle checkpoint kinase Bub1--kinetochore subunit Knl1 interaction . . . . . . . . . . . . . . 451 Julien Espeut and Dhanya K. Cheerambathur and Lenno Krenning and Karen Oegema and Arshad Desai Microtubule binding by KNL-1 contributes to spindle checkpoint silencing at the kinetochore . . . . . . . . . . . . . . 469 Kevin Moreau and Brinda Ravikumar and Claudia Puri and David C. Rubinsztein Arf6 promotes autophagosome formation via effects on phosphatidylinositol 4,5-bisphosphate and phospholipase D . . 483 Nadine Bakkar and Katherine Ladner and Benjamin D. Canan and Sandya Liyanarachchi and Naresh C. Bal and Meghna Pant and Muthu Periasamy and Qiutang Li and Paul M. L. Janssen and Denis C. Guttridge IKK$ \alpha $ and alternative NF-$ \kappa $B regulate PGC-1$ \beta $ to promote oxidative muscle metabolism . . 497 Anat Florentin and Eli Arama Caspase levels and execution efficiencies determine the apoptotic potential of the cell . . . . . . . . . 513 Xu Chen and Barry Ganetzky A neuropeptide signaling pathway regulates synaptic growth in \bionameDrosophila . . . . . . . . . . . 529 Ana Maria Rojas and Gloria Fuentes and Antonio Rausell and Alfonso Valencia The Ras protein superfamily: Evolutionary tree and role of conserved amino acids . . . . . . . . . . . . . . 545
Mitch Leslie Aurora B is no Ska fan . . . . . . . . . ?? Mitch Leslie Vinculin minds the gap . . . . . . . . . ?? Mitch Leslie As the sperm turns . . . . . . . . . . . ?? Mitch Leslie Fly wing cells choose sides . . . . . . 549 Caitlin Sedwick Orna Cohen--Fix: Playing with nuclear morphology . . . . . . . . . . . . . . . 550 Ryan S. King and Phillip A. Newmark The cell biology of regeneration . . . . 553 Ying Wai Chan and A. Arockia Jeyaprakash and Erich A. Nigg and Anna Santamaria Aurora B controls kinetochore--microtubule attachments by inhibiting Ska complex--KMN network interaction . . . . . . . . . . . . . . 563 Mark S. Hipp and Chetan N. Patel and Kirill Bersuker and Brigit E. Riley and Stephen E. Kaiser and Thomas A. Shaler and Michael Brandeis and Ron R. Kopito Indirect inhibition of 26S proteasome activity in a cellular model of Huntington's disease . . . . . . . . . . 573 Megan Gervasi and Anna Bianchi-Smiraglia and Michael Cummings and Qiao Zheng and Dan Wang and Song Liu and Andrei V. Bakin JunB contributes to Id2 repression and the epithelial--mesenchymal transition in response to transforming growth factor--$ \beta $ . . . . . . . . . . . 589 William J. Gault and Patricio Olguin and Ursula Weber and Marek Mlodzik Drosophila CK1-$ \gamma $, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking . . . . . . . . . . . . . . 605 Sandra Iden and Steve Misselwitz and Swetha S. D. Peddibhotla and Hüseyin Tuncay and Daniela Rehder and Volker Gerke and Horst Robenek and Atsushi Suzuki and Klaus Ebnet aPKC phosphorylates JAM--A at Ser285 to promote cell contact maturation and tight junction formation . . . . . . . . 623 Stephan Huveneers and Joppe Oldenburg and Emma Spanjaard and Gerard van der Krogt and Ilya Grigoriev and Anna Akhmanova and Holger Rehmann and Johan de Rooij Vinculin associates with endothelial VE-cadherin junctions to control force-dependent remodeling . . . . . . . 641 Luis Alvarez and Luru Dai and Benjamin M. Friedrich and Nachiket D. Kashikar and Ingo Gregor and René Pascal and U. Benjamin Kaupp The rate of change in Ca$^{2+}$ concentration controls sperm chemotaxis 653
Ben Short ER stress PERKs up an miRNA . . . . . . ?? Ben Short Simulating segregation . . . . . . . . . ?? Ben Short Meet the neighbors . . . . . . . . . . . ?? Ben Short Directing a chaperone . . . . . . . . . 667 Caitlin Sedwick Sue Biggins: How kinetochores keep control of mitosis . . . . . . . . . . . 668 Richard O. Hynes The evolution of metazoan extracellular matrix . . . . . . . . . . . . . . . . . 671 Steven Bergink and Wendy Toussaint and Martijn S. Luijsterburg and Christoffel Dinant and Sergey Alekseev and Jan H. J. Hoeijmakers and Nico P. Dantuma and Adriaan B. Houtsmuller and Wim Vermeulen Recognition of DNA damage by XPC coincides with disruption of the XPC--RAD23 complex . . . . . . . . . . . 681 Andrew E. Byrd and Ileana V. Aragon and Joseph W. Brewer MicroRNA-30c-2* limits expression of proadaptive factor XBP1 in the unfolded protein response . . . . . . . . . . . . 689 Aymeric Ravel-Chapuis and Guy Bélanger and Ramesh S. Yadava and Mani S. Mahadevan and Luc DesGroseillers and Jocelyn Côté and Bernard J. Jasmin The RNA-binding protein Staufen1 is increased in DM1 skeletal muscle and promotes alternative pre-mRNA splicing 699 Sunghwan Kim and Dionisia P. Sideris and Carolyn S. Sevier and Chris A. Kaiser Balanced Ero1 activation and inactivation establishes ER redox homeostasis . . . . . . . . . . . . . . 713 Scott D. Ryan and Kunal Bhanot and Andrew Ferrier and Yves De Repentigny and Alphonse Chu and Alexandre Blais and Rashmi Kothary Microtubule stability, Golgi organization, and transport flux require dystonin-a2--MAP1B interaction . . . . . 727 Xianying Tang and Bryan St. Germain and Wei-Lih Lee A novel patch assembly domain in Num1 mediates dynein anchoring at the cortex during spindle positioning . . . . . . . 743 Guillaume Gay and Thibault Courtheoux and Céline Reyes and Sylvie Tournier and Yannick Gachet A stochastic model of kinetochore--microtubule attachment accurately describes fission yeast chromosome segregation . . . . . . . . . 757 Sandra Dieni and Tomoya Matsumoto and Martijn Dekkers and Stefanie Rauskolb and Mihai S. Ionescu and Ruben Deogracias and Eckart D. Gundelfinger and Masami Kojima and Sigrun Nestel and Michael Frotscher and Yves-Alain Barde BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons . . . . . . . . . . . . . . . . 775 Anjali A. Sarkar and Irene E. Zohn Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme . . . . . . . . . . . . . . . 789 Kyle J. Roux and Dae In Kim and Manfred Raida and Brian Burke A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells 801
Ben Short Ovastacin cuts off sperm binding . . . . ?? Ben Short $ \gamma $-Tubulin stands up to be counted . . . . . . . . . . . . . . . . ?? Ben Short Leukocytes drop anchor . . . . . . . . . ?? Ben Short Big enough for two . . . . . . . . . . . 3 Caitlin Sedwick Ewald Weibel: an organelle of his very own, and more . . . . . . . . . . . . . 4 David W. Reid and Christopher V. Nicchitta The enduring enigma of nuclear translation . . . . . . . . . . . . . . 7 Balca R. Mardin and Elmar Schiebel Breaking the ties that bind: New advances in centrosome biology . . . . . 11 David Reboutier and Marie-Béreng\`ere Troadec and Jean-Yves Cremet and Kenji Fukasawa and Claude Prigent Nucleophosmin\slash B23 activates Aurora A at the centrosome through phosphorylation of serine 89 . . . . . . 19 Wei Li and Wei Zou and Yihong Yang and Yongping Chai and Baohui Chen and Shiya Cheng and Dong Tian and Xiaochen Wang and Ronald D. Vale and Guangshuo Ou Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell . . . 27 Anna D. Burkart and Bo Xiong and Boris Baibakov and Maria Jiménez-Movilla and Jurrien Dean Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy . . . . . . . . . . . 37 Alexandre David and Brian P. Dolan and Heather D. Hickman and Jonathan J. Knowlton and Giovanna Clavarino and Philippe Pierre and Jack R. Bennink and Jonathan W. Yewdell Nuclear translation visualized by ribosome-bound nascent chain puromycylation . . . . . . . . . . . . . 45 Sarah Erlemann and Annett Neuner and Linda Gombos and Romain Gibeaux and Claude Antony and Elmar Schiebel An extended $ \gamma $-tubulin ring functions as a stable platform in microtubule nucleation . . . . . . . . . 59 Andrea K. H. Stavoe and Daniel A. Colón-Ramos Netrin instructs synaptic vesicle clustering through Rac GTPase, MIG-10, and the actin cytoskeleton . . . . . . . 75 Steph D. Anastasia and Duy Linh Nguyen and Vu Thai and Melissa Meloy and Tracy MacDonough and Douglas R. Kellogg A link between mitotic entry and membrane growth suggests a novel model for cell size control . . . . . . . . . 89 Erik S. Welf and Shoeb Ahmed and Heath E. Johnson and Adam T. Melvin and Jason M. Haugh Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism . . . . . . . . 105 Jacob Rullo and Henry Becker and Sharon J. Hyduk and Janice C. Wong and Genevieve Digby and Pamma D. Arora and Adrianet Puig Cano and John Hartwig and Christopher A. McCulloch and Myron I. Cybulsky Actin polymerization stabilizes $ \alpha $4$ \beta $1 integrin anchors that mediate monocyte adhesion . . . . . . . 115 Masamichi Nagae and Suyong Re and Emiko Mihara and Terukazu Nogi and Yuji Sugita and Junichi Takagi Crystal structure of $ \alpha $5$ \beta $1 integrin ectodomain: Atomic details of the fibronectin receptor . . . . . . 131 Georg H. H. Borner and Robin Antrobus and Jennifer Hirst and Gary S. Bhumbra and Patrycja Kozik and Lauren P. Jackson and Daniela A. Sahlender and Margaret S. Robinson Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles . . . . . . . . . . . . 141
Mitch Leslie RNF169 flips the repair switch . . . . . ?? Mitch Leslie BRCA1 touches up microRNAs . . . . . . . ?? Mitch Leslie Wait, save that integrin! . . . . . . . ?? Mitch Leslie Playing with Arp2/3 uncovers cellular rudder . . . . . . . . . . . . . . . . . 163 Caitlin Sedwick Yoshinori Ohsumi: Autophagy from beginning to end . . . . . . . . . . . . 164 Harry T. Orr Cell biology of spinocerebellar ataxia 167 Jannie Rendtlew Danielsen and Lou Klitgaard Povlsen and Bine Hare Villumsen and Werner Streicher and Jakob Nilsson and Mats Wikström and Simon Bekker-Jensen and Niels Mailand DNA damage--inducible SUMOylation of HERC2 promotes RNF8 binding via a novel SUMO-binding Zinc finger . . . . . . . . 179 Maria Poulsen and Claudia Lukas and Jiri Lukas and Simon Bekker-Jensen and Niels Mailand Human RNF169 is a negative regulator of the ubiquitin-dependent response to DNA double-strand breaks . . . . . . . . . . 189 Shinji Kawai and Atsuo Amano BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex . . . 201 Kamran Anwar and Robin W. Klemm and Amanda Condon and Katharina N. Severin and Miao Zhang and Rodolfo Ghirlando and Junjie Hu and Tom A. Rapoport and William A. Prinz The dynamin-like GTPase Sey1p mediates homotypic ER fusion in S. cerevisiae . . 209 Florian Steinberg and Kate J. Heesom and Mark D. Bass and Peter J. Cullen SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways . . . . . . . . . 219 Sarah B. Domnitz and Michael Wagenbach and Justin Decarreau and Linda Wordeman MCAK activity at microtubule tips regulates spindle microtubule length to promote robust kinetochore attachment 231 Praveen Suraneni and Boris Rubinstein and Jay R. Unruh and Michael Durnin and Dorit Hanein and Rong Li The Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration . . . . . . . 239 William C. Drosopoulos and Settapong T. Kosiyatrakul and Zi Yan and Simone G. Calderano and Carl L. Schildkraut Human telomeres replicate using chromosome-specific, rather than universal, replication programs . . . . 253 Martijn S. Luijsterburg and Michael Lindh and Klara Acs and Mischa G. Vrouwe and Alex Pines and Haico van Attikum and Leon H. Mullenders and Nico P. Dantuma DDB2 promotes chromatin decondensation at UV-induced DNA damage . . . . . . . . 267 Paula Martínez and Juana M. Flores and Maria A. Blasco 53BP1 deficiency combined with telomere dysfunction activates ATR-dependent DNA damage response . . . . . . . . . . . . 283 Kristin L. Boswell and Declan J. James and Joseph M. Esquibel and Stephen Bruinsma and Ryutaro Shirakawa and Hisanori Horiuchi and Thomas F. J. Martin Munc13-4 reconstitutes calcium-dependent SNARE-mediated membrane fusion . . . . . 301 Camille Enjolras and Joëlle Thomas and Brigitte Chhin and Elisabeth Cortier and Jean-Luc Duteyrat and Fabien Soulavie and Maurice J. Kernan and Anne Laurençon and Bénédicte Durand Drosophila chibby is required for basal body formation and ciliogenesis but not for Wg signaling . . . . . . . . . . . . 313 Arthur P. H. de Jong and Sabine K. Schmitz and Ruud F. G. Toonen and Matthijs Verhage Dendritic position is a major determinant of presynaptic strength . . 327
Ben Short The APC/C gets Trim'd down for apoptosis ?? Ben Short Prion puts yeast cells under arrest . . ?? Ben Short Ubiquitin isn't ubiquitous in receptor trafficking . . . . . . . . . . . . . . ?? Ben Short Trichoplein keeps primary cilia silent 341 Caitlin Sedwick Coleen Murphy: How to stay young at heart, body, and mind . . . . . . . . . 342 Elizabeth H. Williams and Pamela A. Carpentier and Tom Misteli Minimizing the ``Re'' in Review . . . . 345 Michael Sixt Cell migration: Fibroblasts find a new way to get ahead . . . . . . . . . . . . 347 Deok-Ho Kim and Paolo P. Provenzano and Chris L. Smith and Andre Levchenko Matrix nanotopography as a regulator of cell function . . . . . . . . . . . . . 351 Nai-Jia Huang and Liguo Zhang and Wanli Tang and Chen Chen and Chih-Sheng Yang and Sally Kornbluth The Trim39 ubiquitin ligase inhibits APC/C$^{\rm Cdh1}$-mediated degradation of the Bax activator MOAP-1 . . . . . . 361 Sebastian Treusch and Susan Lindquist An intrinsically disordered yeast prion arrests the cell cycle by sequestering a spindle pole body component . . . . . . 369 Jeremy D. Rotty and Pierre A. Coulombe A wound-induced keratin inhibits Src activity during keratinocyte migration and tissue repair . . . . . . . . . . . 381 Akihito Inoko and Makoto Matsuyama and Hidemasa Goto and Yuki Ohmuro-Matsuyama and Yuko Hayashi and Masato Enomoto and Miho Ibi and Takeshi Urano and Shigenobu Yonemura and Tohru Kiyono and Ichiro Izawa and Masaki Inagaki Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells . . . . . . . . . . . . . . . . . 391 Michael R. Dores and Buxin Chen and Huilan Lin and Unice J. K. Soh and May M. Paing and William A. Montagne and Timo Meerloo and JoAnn Trejo ALIX binds a YPX$_3$ L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting . . . . . . . . . 407 Jeanine Pignatelli and David A. Tumbarello and Ronald P. Schmidt and Christopher E. Turner Hic-5 promotes invadopodia formation and invasion during TGF-$ \beta $-induced epithelial--mesenchymal transition . . . 421 Ryan J. Petrie and Núria Gavara and Richard S. Chadwick and Kenneth M. Yamada Nonpolarized signaling reveals two distinct modes of $3$D cell migration 439 Arun Anantharam and Bibiana Onoa and Robert H. Edwards and Ronald W. Holz and Daniel Axelrod Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM . . . . . 457
Ben Short Mitochondrial morphology shapes differentiation . . . . . . . . . . . . ?? Ben Short Putting a ``cap'' on clathrin-mediated endocytosis . . . . . . . . . . . . . . ?? Ben Short Protrusion drives osteoclast fusion . . ?? Ben Short miR-34s keep osteoblasts bone idle . . . 461 Caitlin Sedwick Fernando Camargo: No limits to learning about stem cells . . . . . . . . . . . . 462 Kathleen C. Suozzi and Xiaoyang Wu and Elaine Fuchs Spectraplakins: Master orchestrators of cytoskeletal dynamics . . . . . . . . . 465 Jennifer Zanet and Asier Jayo and Serge Plaza and Tom Millard and Maddy Parsons and Brian Stramer Fascin promotes filopodia formation independent of its role in actin bundling . . . . . . . . . . . . . . . . 477 Kasturi Mitra and Richa Rikhy and Mary Lilly and Jennifer Lippincott-Schwartz DRP1-dependent mitochondrial fission initiates follicle cell differentiation during \bionameDrosophila oogenesis . . 487 Andrew I. Shevchuk and Pavel Novak and Marcus Taylor and Ivan A. Diakonov and Azza Ziyadeh-Isleem and Marc Bitoun and Pascale Guicheney and Max J. Lab and Julia Gorelik and Christien J. Merrifield and David Klenerman and Yuri E. Korchev An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy . . . . . . . 499 Jianwen Wei and Yu Shi and Lihua Zheng and Bin Zhou and Hiroyuki Inose and Ji Wang and X. Edward Guo and Rudolf Grosschedl and Gerard Karsenty miR-34s inhibit osteoblast proliferation and differentiation in the mouse by targeting SATB2 . . . . . . . . . . . . 509 Jose M. Celedon and Kenneth Cline Stoichiometry for binding and transport by the twin arginine translocation system . . . . . . . . . . . . . . . . . 523 Yusuke Kageyama and Zhongyan Zhang and Ricardo Roda and Masahiro Fukaya and Junko Wakabayashi and Nobunao Wakabayashi and Thomas W. Kensler and P. Hemachandra Reddy and Miho Iijima and Hiromi Sesaki Mitochondrial division ensures the survival of postmitotic neurons by suppressing oxidative damage . . . . . . 535 Tsukasa Oikawa and Masaaki Oyama and Hiroko Kozuka-Hata and Shunsuke Uehara and Nobuyuki Udagawa and Hideyuki Saya and Koichi Matsuo Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell--cell fusion . . . . . . . 553
Mitch Leslie Talin gives integrins a nudge . . . . . ?? Mitch Leslie Recycling endosomes feed autophagy . . . ?? Mitch Leslie Unfinished basement membrane spurs developmental disorder . . . . . . . . . ?? Mitch Leslie Nck by the numbers . . . . . . . . . . . 571 Caitlin Sedwick Graça Raposo: Melanosomes, more than skin deep . . . . . . . . . . . . . . . . . . 572 Alexandra Van Keymeulen and Cédric Blanpain Tracing epithelial stem cells during development, homeostasis, and repair . . 575 Mandy E. W. Janssen and HongJun Liu and Niels Volkmann and Dorit Hanein The C-terminal tail domain of metavinculin, vinculin's splice variant, severs actin filaments . . . . . . . . . 585 Michael Gebert and Sandra G. Schrempp and Carola S. Mehnert and Anna K. Heißwolf and Silke Oeljeklaus and Raffaele Ieva and Maria Bohnert and Karina von der Malsburg and Sebastian Wiese and Thomas Kleinschroth and Carola Hunte and Helmut E. Meyer and Ilka Haferkamp and Bernard Guiard and Bettina Warscheid and Nikolaus Pfanner and Martin van der Laan Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes . . . . . . . . . . . 595 Chungho Kim and Feng Ye and Xiaohui Hu and Mark H. Ginsberg Talin activates integrins by altering the topology of the $ \beta $ transmembrane domain . . . . . . . . . . 605 Joseph K. Hsu and Tao Lin and Robert Y. L. Tsai Nucleostemin prevents telomere damage by promoting PML--IV recruitment to SUMOylated TRF1 . . . . . . . . . . . . 613 Tatsuyuki Chiyoda and Naoyuki Sugiyama and Takatsune Shimizu and Hideaki Naoe and Yusuke Kobayashi and Jo Ishizawa and Yoshimi Arima and Hiroshi Tsuda and Masaaki Ito and Kozo Kaibuchi and Daisuke Aoki and Yasushi Ishihama and Hideyuki Saya and Shinji Kuninaka LATS1\slash WARTS phosphorylates MYPT1 to counteract PLK1 and regulate mammalian mitotic progression . . . . . 625 Jonathon A. Ditlev and Paul J. Michalski and Greg Huber and Gonzalo M. Rivera and William A. Mohler and Leslie M. Loew and Bruce J. Mayer Stoichiometry of Nck-dependent actin polymerization in living cells . . . . . 643 Andrea Longatti and Christopher A. Lamb and Minoo Razi and Shin-ichiro Yoshimura and Francis A. Barr and Sharon A. Tooze TBC1D14 regulates autophagosome formation via Rab11- and ULK1-positive recycling endosomes . . . . . . . . . . 659 Daiji Kiyozumi and Makiko Takeichi and Itsuko Nakano and Yuya Sato and Tomohiko Fukuda and Kiyotoshi Sekiguchi Basement membrane assembly of the integrin $ \alpha $8$ \beta $1 ligand nephronectin requires Fraser syndrome--associated proteins . . . . . 677
Ben Short Protrusion provides a prognosis . . . . ?? Ben Short The ribosome runs a systems test . . . . ?? Ben Short IFT-A goes both ways . . . . . . . . . . ?? Ben Short Talin is cut out for intercellular adhesion . . . . . . . . . . . . . . . . 693 Caitlin Sedwick Alexey Khodjakov: Diving into the mitotic apparatus . . . . . . . . . . . 694 Francesc R. Garcia-Gonzalo and Jeremy F. Reiter Scoring a backstage pass: Mechanisms of ciliogenesis and ciliary access . . . . 697 Cesare Orlandi and Ekaterina Posokhova and Ikuo Masuho and Thomas A. Ray and Nazarul Hasan and Ronald G. Gregg and Kirill A. Martemyanov GPR158\slash 179 regulate G protein signaling by controlling localization and activity of the RGS7 complexes . . . 711 Aaron S. Meyer and Shannon K. Hughes-Alford and Jennifer E. Kay and Amalchi Castillo and Alan Wells and Frank B. Gertler and Douglas A. Lauffenburger 2D protrusion but not motility predicts growth factor--induced cancer cell migration in $3$D collagen . . . . . . . 721 Jian Qu and J. Michael Bishop Nucleostemin maintains self-renewal of embryonic stem cells and promotes reprogramming of somatic cells to pluripotency . . . . . . . . . . . . . . 731 Cyril Bussiere and Yaser Hashem and Sucheta Arora and Joachim Frank and Arlen W. Johnson Integrity of the P-site is probed during maturation of the 60S ribosomal subunit 747 Eric M. Rubenstein and Stefan G. Kreft and Wesley Greenblatt and Robert Swanson and Mark Hochstrasser Aberrant substrate engagement of the ER translocon triggers degradation by the Hrd1 ubiquitin ligase . . . . . . . . . 761 Alexander Babich and Shuixing Li and Roddy S. O'Connor and Michael C. Milone and Bruce D. Freedman and Janis K. Burkhardt F-actin polymerization and retrograde flow drive sustained PLC$ \gamma $1 signaling during T cell activation . . . 775 Karel F. Liem and Alyson Ashe and Mu He and Peter Satir and Jennifer Moran and David Beier and Carol Wicking and Kathryn V. Anderson The IFT--A complex regulates Shh signaling through cilia structure and membrane protein trafficking . . . . . . 789 Athina-Myrto Chioni and Richard Grose FGFR1 cleavage and nuclear translocation regulates breast cancer cell behavior 801 Fangliang Zhang and Sougata Saha and Anna Kashina Arginylation-dependent regulation of a proteolytic product of talin is essential for cell--cell adhesion . . . 819 Sophie Lutter and Sherry Xie and Florence Tatin and Taija Makinen Smooth muscle--endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation 837
Mitch Leslie Cohesin sticks to it during meiosis . . ?? Mitch Leslie Why yeast mothers and daughters don't share . . . . . . . . . . . . . . . . . ?? Mitch Leslie Lis1 cuts its work short . . . . . . . . ?? Mitch Leslie Asters push embryonic nuclei to the brink . . . . . . . . . . . . . . . . . 853 Caitlin Sedwick Markus Grebe: Bringing plant polarity to light . . . . . . . . . . . . . . . . . 854 Shiyu Wang and Randal J. Kaufman The impact of the unfolded protein response on human disease . . . . . . . 857 Arne Nedergaard Kousholt and Kasper Fugger and Saskia Hoffmann and Brian D. Larsen and Tobias Menzel and Alessandro A. Sartori and Claus Storgaard Sòrensen CtIP-dependent DNA resection is required for DNA damage checkpoint maintenance but not initiation . . . . . . . . . . . 869 Elena Llano and Yurema Herrán and Ignacio García-Tuñón and Cristina Gutiérrez-Caballero and Enrique de Álava and José Luis Barbero and John Schimenti and Dirk G. de Rooij and Manuel Sánchez-Martín and Alberto M. Pendás Meiotic cohesin complexes are essential for the formation of the axial element in mice . . . . . . . . . . . . . . . . 877 Ivo A. Telley and Imre Gáspár and Anne Ephrussi and Thomas Surrey Aster migration determines the length scale of nuclear separation in the \bionameDrosophila syncytial embryo . . 887 Boris Baibakov and Nathan A. Boggs and Belinda Yauger and Galina Baibakov and Jurrien Dean Human sperm bind to the N-terminal domain of ZP2 in humanized zonae pellucidae in transgenic mice . . . . . 897 Ssang-Taek Lim and Nichol L. G. Miller and Xiao Lei Chen and Isabelle Tancioni and Colin T. Walsh and Christine Lawson and Sean Uryu and Sara M. Weis and David A. Cheresh and David D. Schlaepfer Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression 907 Barbara Boettcher and Tatiana T. Marquez-Lago and Mathias Bayer and Eric L. Weiss and Yves Barral Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis 921 Qing Yang and Xiao-Feng Zhang and Thomas D. Pollard and Paul Forscher Arp2\slash 3 complex-dependent actin networks constrain myosin II function in driving retrograde actin flow . . . . . 939 Katherine C. Rank and Chun Ju Chen and Julia Cope and Ken Porche and Andreas Hoenger and Susan P. Gilbert and Ivan Rayment Kar3Vik1, a member of the Kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern . . . . . . 957 Martin J. Egan and Kaeling Tan and Samara L. Reck-Peterson Lis1 is an initiation factor for dynein-driven organelle transport . . . 971 Yasuhito Onodera and Jin-Min Nam and Ari Hashimoto and Jim C. Norman and Hiroki Shirato and Shigeru Hashimoto and Hisataka Sabe Rab5c promotes AMAP1--PRKD2 complex formation to enhance $ \beta $1 integrin recycling in EGF-induced cancer invasion 983 Catherine E. Winbanks and Kate L. Weeks and Rachel E. Thomson and Patricio V. Sepulveda and Claudia Beyer and Hongwei Qian and Justin L. Chen and James M. Allen and Graeme I. Lancaster and Mark A. Febbraio and Craig A. Harrison and Julie R. McMullen and Jeffrey S. Chamberlain and Paul Gregorevic Follistatin-mediated skeletal muscle hypertrophy is regulated by Smad3 and mTOR independently of myostatin . . . . 997 Jamie L. Marshall and Johan Holmberg and Eric Chou and Amber C. Ocampo and Jennifer Oh and Joy Lee and Angela K. Peter and Paul T. Martin and Rachelle H. Crosbie-Watson Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration . . . . . . . . . . 1009 Steven M. Claypool and Yavuz Oktay and Pinmanee Boontheung and Joseph A. Loo and Carla M. Koehler Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane . . . . . . 1029
Ben Short The Fuzzy logistics of intraflagellar transport . . . . . . . . . . . . . . . ?? Ben Short Organelles compete for their inheritance ?? Ben Short Formin' muscle sarcomeres . . . . . . . ?? Ben Short Schwann cells aid regeneration project 3 Caitlin Sedwick Jiri Lukas: Visualizing genome integrity maintenance . . . . . . . . . . . . . . 4 Ilya Bezprozvanny Presenilins: a novel link between intracellular calcium signaling and lysosomal function? . . . . . . . . . . 7 Hugues de Thé and Morgane Le Bras and Valérie Lallemand-Breitenbach Acute promyelocytic leukemia, arsenic, and PML bodies . . . . . . . . . . . . . 11 Katrijn Coen and Ronald S. Flannagan and Szilvia Baron and Luciene R. Carraro-Lacroix and Dong Wang and Wendy Vermeire and Christine Michiels and Sebastian Munck and Veerle Baert and Shuzo Sugita and Frank Wuytack and Peter Robin Hiesinger and Sergio Grinstein and Wim Annaert Lysosomal calcium homeostasis defects, not proton pump defects, cause endo-lysosomal dysfunction in PSEN-deficient cells . . . . . . . . . . 23 Eric R. Brooks and John B. Wallingford Control of vertebrate intraflagellar transport by the planar cell polarity effector Fuz . . . . . . . . . . . . . . 37 Yi Fan and Sandeepa M. Eswarappa and Masahiro Hitomi and Paul L. Fox Myo1c facilitates G-actin transport to the leading edge of migrating endothelial cells . . . . . . . . . . . 47 Carl Laflamme and Gloria Assaker and Damien Ramel and Jonas F. Dorn and Desmond She and Paul S. Maddox and Gregory Emery Evi5 promotes collective cell migration through its Rab--GAP activity . . . . . 57 P. Taylor Eves and Yui Jin and Matthew Brunner and Lois S. Weisman Overlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes . . . . . . . . . . . . . . . . 69 Lei Mi-Mi and SarahBeth Votra and Kenneth Kemphues and Anthony Bretscher and David Pruyne Z-line formins promote contractile lattice growth and maintenance in striated muscles of \bionameC. elegans 87 Yanmei Qi and Xiaoxiang Tian and Jie Liu and Yaling Han and Alan M. Graham and M. Celeste Simon and Josef M. Penninger and Peter Carmeliet and Shaohua Li Bnip3 and AIF cooperate to induce apoptosis and cavitation during epithelial morphogenesis . . . . . . . . 103 Anjali Gupta and Dennis R. Diener and Priyanka Sivadas and Joel L. Rosenbaum and Pinfen Yang The versatile molecular complex component LC8 promotes several distinct steps of flagellar assembly . . . . . . 115 Xavier Fontana and Mariya Hristova and Clive Da Costa and Smriti Patodia and Laura Thei and Milan Makwana and Bradley Spencer-Dene and Morwena Latouche and Rhona Mirsky and Kristjan R. Jessen and Rüdiger Klein and Gennadij Raivich and Axel Behrens c-Jun in Schwann cells promotes axonal regeneration and motoneuron survival via paracrine signaling . . . . . . . . . . 127 Barbara Boettcher and Tatiana T. Marquez-Lago and Mathias Bayer and Eric L. Weiss and Yves Barral Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis 143
Ben Short Sds22 and Repo--Man keep anaphase chromosomes moving . . . . . . . . . . . ?? Ben Short Microvillar scaffold is only temporary ?? Ben Short BubR1 shows self-control . . . . . . . . ?? Ben Short PI(3,5)P$_2$ sows the seeds of plant growth . . . . . . . . . . . . . . . . . 147 Caitlin Sedwick Karen Vousden: Getting the big picture on p53 . . . . . . . . . . . . . . . . . 148 Fulvio Reggiori and Sharon A. Tooze Autophagy regulation through Atg9 traffic . . . . . . . . . . . . . . . . 151 Yue Xiong and Kun-Liang Guan Mechanistic insights into the regulation of metabolic enzymes by acetylation . . 155 Akihiro Morimoto and Hiroki Shibuya and Xiaoqiang Zhu and Jihye Kim and Kei-ichiro Ishiguro and Min Han and Yoshinori Watanabe A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis . . . 165 Claudia Wurzenberger and Michael Held and Michael A. Lampson and Ina Poser and Anthony A. Hyman and Daniel W. Gerlich Sds22 and Repo--Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores . . . . . . . . . 173 Kirti Magudia and Aurelia Lahoz and Alan Hall K-Ras and B--Raf oncogenes inhibit colon epithelial polarity establishment through up-regulation of c-myc . . . . . 185 Damien Garbett and Anthony Bretscher PDZ interactions regulate rapid turnover of the scaffolding protein EBP50 in microvilli . . . . . . . . . . . . . . . 195 Yige Guo and Christine Kim and Sana Ahmad and Jiayin Zhang and Yinghui Mao CENP-E-dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint . . 205 Hayashi Yamamoto and Soichiro Kakuta and Tomonobu M. Watanabe and Akira Kitamura and Takayuki Sekito and Chika Kondo-Kakuta and Rie Ichikawa and Masataka Kinjo and Yoshinori Ohsumi Atg9 vesicles are an important membrane source during early steps of autophagosome formation . . . . . . . . 219 Peter A. C. van Gisbergen and Ming Li and Shu-Zon Wu and Magdalena Bezanilla Class II formin targeting to the cell cortex by binding PI(3,5)P$_2$ is essential for polarized growth . . . . . 235 Enora Moutin and Fabrice Raynaud and Jonathan Roger and Emilie Pellegrino and Vincent Homburger and Federica Bertaso and Vincent Ollendorff and Joël Bockaert and Laurent Fagni and Julie Perroy Dynamic remodeling of scaffold interactions in dendritic spines controls synaptic excitability . . . . . 251
Mitch Leslie Fungal pores feel the pull . . . . . . . ?? Mitch Leslie Chaperones team up twice . . . . . . . . ?? Mitch Leslie CLASPing microtubules to the membrane ?? Mitch Leslie Outgrowing meiosis . . . . . . . . . . . 267 Caitlin Sedwick Barry Thompson: The delicate choreography in growing epithelia . . . 268 Elizabeth H. Williams and Pamela Carpentier and Tom Misteli The JCB DataViewer scales up . . . . . . 271 Yosef Shiloh and Yael Ziv The ATM protein: The importance of being active . . . . . . . . . . . . . . . . . 273 David Pincus and Peter Walter A first line of defense against ER stress . . . . . . . . . . . . . . . . . 277 Ir\`ene Baccelli and Andreas Trumpp The evolving concept of cancer and metastasis stem cells . . . . . . . . . 281 Jeremy A. Daniel and Manuela Pellegrini and Baeck-Seung Lee and Zhi Guo and Darius Filsuf and Natalya V. Belkina and Zhongsheng You and Tanya T. Paull and Barry P. Sleckman and Lionel Feigenbaum and André Nussenzweig Loss of ATM kinase activity leads to embryonic lethality in mice . . . . . . 295 Kenta Yamamoto and Yunyue Wang and Wenxia Jiang and Xiangyu Liu and Richard L. Dubois and Chyuan-Sheng Lin and Thomas Ludwig and Christopher J. Bakkenist and Shan Zha Kinase-dead ATM protein causes genomic instability and early embryonic lethality in mice . . . . . . . . . . . 305 Hiroaki Yajima and Toshihiko Ogura and Ryo Nitta and Yasushi Okada and Chikara Sato and Nobutaka Hirokawa Conformational changes in tubulin in GMPCPP and GDP-taxol microtubules observed by cryoelectron microscopy . . 315 Yao Wu and Yiwen Gu and Mary K. Morphew and Jun Yao and Felix L. Yeh and Min Dong and Edwin R. Chapman All three components of the neuronal SNARE complex contribute to secretory vesicle docking . . . . . . . . . . . . 323 Julien Bellis and Isabelle Duluc and Béatrice Romagnolo and Christine Perret and Maree C. Faux and Denis Dujardin and Caroline Formstone and Sally Lightowler and Robert G. Ramsay and Jean-Noël Freund and Jan R. De Mey The tumor suppressor Apc controls planar cell polarities central to gut homeostasis . . . . . . . . . . . . . . 331 Gero Steinberg and Martin Schuster and Ulrike Theisen and Sreedhar Kilaru and Andrew Forge and Magdalena Martin-Urdiroz Motor-driven motility of fungal nuclear pores organizes chromosomes and fosters nucleocytoplasmic transport . . . . . . 343 Aurélien Courtois and Melina Schuh and Jan Ellenberg and Takashi Hiiragi The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development . . . . . . . . . 357 Joseph E. Chambers and Kseniya Petrova and Giulia Tomba and Michele Vendruscolo and David Ron ADP ribosylation adapts an ER chaperone response to short-term fluctuations in unfolded protein load . . . . . . . . . 371 Juliane Winkler and Jens Tyedmers and Bernd Bukau and Axel Mogk Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation . . . . . . . . 387 Karen E. Moreira and Sebastian Schuck and Bianca Schrul and Florian Fröhlich and James B. Moseley and Tobias C. Walther and Peter Walter Seg1 controls eisosome assembly and shape . . . . . . . . . . . . . . . . . 405 Nadine Schmidt and Sreya Basu and Stefan Sladecek and Sabrina Gatti and Jeffrey van Haren and Susan Treves and Jan Pielage and Niels Galjart and Hans Rudolf Brenner Agrin regulates CLASP2-mediated capture of microtubules at the neuromuscular junction synaptic membrane . . . . . . . 421 Jonathan C. Reed and Britta Molter and Clair D. Geary and John McNevin and Julie McElrath and Samina Giri and Kevin C. Klein and Jaisri R. Lingappa HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly . . . . . . 439 Frank G. A. Faas and M. Cristina Avramut and Bernard M. van den Berg and A. Mieke Mommaas and Abraham J. Koster and Raimond B. G. Ravelli Virtual nanoscopy: Generation of ultra-large high resolution electron microscopy maps . . . . . . . . . . . . 457
Ben Short WASP takes the sting out of SCAR mutants ?? Ben Short GW220 seals the fate of mRNAs . . . . . ?? Ben Short Centrosomes are PKA's sensitive spot . . ?? Ben Short Clathrin helps maintain the centrosome's integrity . . . . . . . . . . . . . . . 473 Caitlin Sedwick Bruce Nicklas: Pioneering studies on spindle forces . . . . . . . . . . . . . 474 Stuart Cane and Thomas J. Maresca Balancing the kinetochore ledger . . . . 477 Samantha Stehbens and Torsten Wittmann Targeting and transport: How microtubules control focal adhesion dynamics . . . . . . . . . . . . . . . . 481 Dan Liu and Olga Davydenko and Michael A. Lampson Polo-like kinase-1 regulates kinetochore--microtubule dynamics and spindle checkpoint silencing . . . . . . 491 Douwe M. Veltman and Jason S. King and Laura M. Machesky and Robert H. Insall SCAR knockouts in \bionameDictyostelium: WASP assumes SCAR's position and upstream regulators in pseudopods . . . 501 Peer Papior and José M. Arteaga-Salas and Thomas Günther and Adam Grundhoff and Aloys Schepers Open chromatin structures regulate the efficiencies of pre--RC formation and replication initiation in Epstein--Barr virus . . . . . . . . . . . . . . . . . 509 Virginia Castilla-Llorente and Lee Spraggon and Miwako Okamura and Saif Naseeruddin and Matthew Adamow and Sarah Qamar and Jidong Liu Mammalian GW220/TNGW1 is essential for the formation of GW/P bodies containing miRISC . . . . . . . . . . . . . . . . . 529 Yu Chen and Yan Wang and Jinzhong Zhang and Yongqiang Deng and Li Jiang and Eli Song and Xufeng S. Wu and John A. Hammer and Tao Xu and Jennifer Lippincott-Schwartz Rab10 and myosin--Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes . . . . . . 545 Jonathan N. Thon and Christopher G. Peters and Kellie R. Machlus and Rukhsana Aslam and Jesse Rowley and Hannah Macleod and Matthew T. Devine and Tobias A. Fuchs and Andrew S. Weyrich and John W. Semple and Robert Flaumenhaft and Joseph E. Italiano T granules in human platelets function in TLR9 organization and signaling . . . 561 Shenhav Cohen and Bo Zhai and Steven P. Gygi and Alfred L. Goldberg Ubiquitylation by Trim32 causes coupled loss of desmin, Z-bands, and thin filaments in muscle atrophy . . . . . . 575 Amy B. Foraker and Stéphane M. Camus and Timothy M. Evans and Sophia R. Majeed and Chih-Ying Chen and Sabrina B. Taner and Ivan R. Corrêa and Stephen J. Doxsey and Frances M. Brodsky Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch--TOG . . . . . . . . . 591 Anna Terrin and Stefania Monterisi and Alessandra Stangherlin and Anna Zoccarato and Andreas Koschinski and Nicoletta C. Surdo and Marco Mongillo and Akira Sawa and Niove E. Jordanides and Joanne C. Mountford and Manuela Zaccolo PKA and PDE4D3 anchoring to AKAP9 provides distinct regulation of cAMP signals at the centrosome . . . . . . . 607 Taras Makhnevych and Philip Wong and Oxana Pogoutse and Franco J. Vizeacoumar and Jack F. Greenblatt and Andrew Emili and Walid A. Houry Hsp110 is required for spindle length control . . . . . . . . . . . . . . . . 623 Juan Carlos G. Cortés and Mamiko Sato and Javier Muñoz and M. Belén Moreno and Jose Angel Clemente-Ramos and Mariona Ramos and Hitoshi Okada and Masako Osumi and Angel Durán and Juan Carlos Ribas Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission . . . . . . . . . . . . 637 Stephanie L. Gupton and Daisy Riquelme and Shannon K. Hughes-Alford and Jenny Tadros and Shireen S. Rudina and Richard O. Hynes and Douglas Lauffenburger and Frank B. Gertler Mena binds $ \alpha $5 integrin directly and modulates $ \alpha $5$ \beta $1 function . . . . . . . . . . . . . . . . 657 Ahmed Elbediwy and Ceniz Zihni and Stephen J. Terry and Peter Clark and Karl Matter and Maria S. Balda Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex . . . . . . . . . . . . . 677 Bianca Kraft and Corinna D. Berger and Veronika Wallkamm and Herbert Steinbeisser and Doris Wedlich Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin . . . . . . . . . . 695 Aurélia Vernay and Sébastien Schaub and Isabelle Guillas and Martine Bassilana and Robert A. Arkowitz A steep phosphoinositide bis-phosphate gradient forms during fungal filamentous growth . . . . . . . . . . . . . . . . . 711 Sara K. Olson and Garrett Greenan and Arshad Desai and Thomas Müller-Reichert and Karen Oegema Hierarchical assembly of the eggshell and permeability barrier in \bionameC. elegans . . . . . . . . . . . . . . . . 731 Brian Jenkins and Helena Decker and Marvin Bentley and Julie Luisi and Gary Banker A novel split kinesin assay identifies motor proteins that interact with distinct vesicle populations . . . . . . 749
Ben Short $ \gamma $-Tubulin flips the G1/S switch ?? Ben Short Rad53 keeps watch over mitochondrial DNA ?? Ben Short An enzyme complex helps fatten up lipid droplets . . . . . . . . . . . . . . . . ?? Ben Short Putting SUMO and SoxE into context . . . 765 Caitlin Sedwick Tannishtha Reya: Classic pathways, new views on cancer . . . . . . . . . . . . 766 Tim Davies and Julie C. Canman Stuck in the middle: Rac, adhesion, and cytokinesis . . . . . . . . . . . . . . 769 Volker Brinkmann and Arturo Zychlinsky Neutrophil extracellular traps: Is immunity the second function of chromatin? . . . . . . . . . . . . . . . 773 Heather Edgerton-Morgan and Berl R. Oakley $ \gamma $-Tubulin plays a key role in inactivating APC/C$^{\rm Cdh1}$ at the G$_1$--S boundary . . . . . . . . . . . 785 David G. Crider and Luis J. García-Rodríguez and Pallavi Srivastava and Leonardo Peraza-Reyes and Krishna Upadhyaya and Istvan R. Boldogh and Liza A. Pon Rad53 is essential for a mitochondrial DNA inheritance checkpoint regulating G1 to S progression . . . . . . . . . . . . 793 Pei-Chih Lee and Kimberly M. Taylor-Jaffe and Kara M. Nordin and Maneeshi S. Prasad and Rachel M. Lander and Carole LaBonne SUMOylated SoxE factors recruit Grg4 and function as transcriptional repressors in the neural crest . . . . . . . . . . 799 Fabien Le Grand and Raphaëlle Grifone and Philippos Mourikis and Christophe Houbron and Carine Gigaud and Julien Pujol and Marjorie Maillet and Gilles Pag\`es and Michael Rudnicki and Shahragim Tajbakhsh and Pascal Maire Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration . . . . . . 815 Hadas Elhanany-Tamir and Yanxun V. Yu and Miri Shnayder and Ankit Jain and Michael Welte and Talila Volk Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules . . . . . . . 833 Cornelia Wandke and Marin Barisic and Reinhard Sigl and Veronika Rauch and Frank Wolf and Ana C. Amaro and Chia H. Tan and Antonio J. Pereira and Ulrike Kutay and Helder Maiato and Patrick Meraldi and Stephan Geley Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis . . . . . . . . 847 Ricardo Nunes Bastos and Xenia Penate and Michelle Bates and Dean Hammond and Francis A. Barr CYK4 inhibits Rac1-dependent PAK1 and ARHGEF7 effector pathways during cytokinesis . . . . . . . . . . . . . . 865 Eunyong Park and Tom A. Rapoport Bacterial protein translocation requires only one copy of the SecY complex in vivo . . . . . . . . . . . . . . . . . . 881 Ningyi Xu and Shaobing O. Zhang and Ronald A. Cole and Sean A. McKinney and Fengli Guo and Joel T. Haas and Sudheer Bobba and Robert V. Farese and Ho Yi Mak The FATP1--DGAT2 complex facilitates lipid droplet expansion at the ER--lipid droplet interface . . . . . . . . . . . 895 Khanh Huy Bui and Toshiki Yagi and Ryosuke Yamamoto and Ritsu Kamiya and Takashi Ishikawa Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme 913 Alexia Mahuzier and Helori-Mael Gaudé and Valentina Grampa and Isabelle Anselme and Flora Silbermann and Margot Leroux-Berger and Delphine Delacour and Jerome Ezan and Mireille Montcouquiol and Sophie Saunier and Sylvie Schneider-Maunoury and Christine Vesque Dishevelled stabilization by the ciliopathy protein Rpgrip1l is essential for planar cell polarity . . . . . . . . 927 Stephanie Woo and Michael P. Housley and Orion D. Weiner and Didier Y. R. Stainier Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 . . . . . . . . . . . . . 941
Mitch Leslie Lipins cause a lamina breakdown . . . . ?? Mitch Leslie Crumbs stands united against ROS . . . . ?? Mitch Leslie CaMKII points the way . . . . . . . . . ?? Mitch Leslie Unlocking the sperm's internal compass 955 Caitlin Sedwick Wendell Lim: Exploring the path not chosen . . . . . . . . . . . . . . . . . 956 Alan F. Cowman and Drew Berry and Jake Baum The cellular and molecular basis for malaria parasite invasion of the human red blood cell . . . . . . . . . . . . . 961 Kai Yuan and Jeffrey A. Farrell and Patrick H. O'Farrell Different cyclin types collaborate to reverse the S-phase checkpoint and permit prompt mitosis . . . . . . . . . 973 Moritz Mall and Thomas Walter and Mátyás Gorjánácz and Iain F. Davidson and Thi Bach Nga Ly-Hartig and Jan Ellenberg and Iain W. Mattaj Mitotic lamin disassembly is triggered by lipid-mediated signaling . . . . . . 981 François J.-M. Chartier and Émilie J.-L. Hardy and Patrick Laprise Crumbs limits oxidase-dependent signaling to maintain epithelial integrity and prevent photoreceptor cell death . . . . . . . . . . . . . . . . . 991 Pablo H. Strobl-Mazzulla and Marianne E. Bronner A PHD12--Snail2 repressive complex epigenetically mediates neural crest epithelial-to-mesenchymal transition . . 999 Alejo E. Rodríguez-Fraticelli and Muriel Auzan and Miguel A. Alonso and Michel Bornens and Fernando Martín-Belmonte Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis . . . . . . . . 1011 Laura Andrés-Delgado and Olga M. Antón and Francesca Bartolini and Ana Ruiz-Sáenz and Isabel Correas and Gregg G. Gundersen and Miguel A. Alonso INF2 promotes the formation of detyrosinated microtubules necessary for centrosome reorientation in T cells . . 1025 Anja K. Dunsch and Dean Hammond and Jennifer Lloyd and Lothar Schermelleh and Ulrike Gruneberg and Francis A. Barr Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation 1039 Mado Lemieux and Simon Labrecque and Christian Tardif and Étienne Labrie-Dion and Éric LeBel and Paul De Koninck Translocation of CaMKII to dendritic microtubules supports the plasticity of local synapses . . . . . . . . . . . . . 1055 Nachiket D. Kashikar and Luis Alvarez and Reinhard Seifert and Ingo Gregor and Oliver Jäckle and Michael Beyermann and Eberhard Krause and U. Benjamin Kaupp Temporal sampling, resetting, and adaptation orchestrate gradient sensing in sperm . . . . . . . . . . . . . . . . 1075
Mitch Leslie B23 steals to stop cell-killing pair . . ?? Mitch Leslie microRNA's big role in preventing tumors ?? Mitch Leslie Dynein's helper not so helpful . . . . . ?? Mitch Leslie Cytoskeleton key . . . . . . . . . . . . 3 Caitlin Sedwick Cédric Blanpain: The stories stem cells tell . . . . . . . . . . . . . . . . . . 4 Priya Prakash Budde and Elizabeth H. Williams and Tom Misteli Cell biology: At the center of modern biomedicine . . . . . . . . . . . . . . 7 Leslie B. Gordon and Kan Cao and Francis S. Collins Progeria: Translational insights from cell biology . . . . . . . . . . . . . . 9 Matthew J. Davis and Joseph Schlessinger The genesis of Zelboraf: Targeting mutant B--Raf in melanoma . . . . . . . 15 Frank Rigo and Yimin Hua and Adrian R. Krainer and C. Frank Bennett Antisense-based therapy for the treatment of spinal muscular atrophy . . 21 Daisuke Izawa and Jonathon Pines Mad2 and the APC/C compete for the same site on Cdc20 to ensure proper chromosome segregation . . . . . . . . . 27 Alexandre R. Gingras and Jian J. Liu and Mark H. Ginsberg Structural basis of the junctional anchorage of the cerebral cavernous malformations complex . . . . . . . . . 39 Tobias Ulbricht and Mohammad Alzrigat and Almut Horch and Nina Reuter and Anna von Mikecz and Viktor Steimle and Eberhard Schmitt and Oliver H. Krämer and Thomas Stamminger and Peter Hemmerich PML promotes MHC class II gene expression by stabilizing the class II transactivator . . . . . . . . . . . . . 49 Sang Bae Lee and Chung Kwon Kim and Kyung-Hoon Lee and Jee-Yin Ahn S-nitrosylation of B23/nucleophosmin by GAPDH protects cells from the SIAH1--GAPDH death cascade . . . . . . . 65 Matteo J. Marzi and Eleonora M. R. Puggioni and Valentina Dall'Olio and Gabriele Bucci and Loris Bernard and Fabrizio Bianchi and Marco Crescenzi and Pier Paolo Di Fiore and Francesco Nicassio Differentiation-associated microRNAs antagonize the Rb--E2F pathway to restrict proliferation . . . . . . . . . 77 Sachin Kotak and Coralie Busso and Pierre Gönczy Cortical dynein is critical for proper spindle positioning in human cells . . . 97 Qing-Tao Shen and Peter P. Hsiue and Charles V. Sindelar and Matthew D. Welch and Kenneth G. Campellone and Hong-Wei Wang Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling . . . . . . . . . . 111 Marta Blamowska and Walter Neupert and Kai Hell Biogenesis of the mitochondrial Hsp70 chaperone . . . . . . . . . . . . . . . 125 Steffi Gruschke and Katharina Römpler and Markus Hildenbeutel and Kirsten Kehrein and Inge Kühl and Nathalie Bonnefoy and Martin Ott The Cbp3--Cbp6 complex coordinates cytochrome b synthesis with bc$_1$ complex assembly in yeast mitochondria 137 Benjamin D. Engel and Hiroaki Ishikawa and Kimberly A. Wemmer and Stefan Geimer and Ken-ichi Wakabayashi and Masafumi Hirono and Branch Craige and Gregory J. Pazour and George B. Witman and Ritsu Kamiya and Wallace F. Marshall The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function . . . . . . . 151 Rui Duan and Peng Jin and Fengbao Luo and Guofeng Zhang and Nathan Anderson and Elizabeth H. Chen Group I PAKs function downstream of Rac to promote podosome invasion during myoblast fusion in vivo . . . . . . . . 169
Ben Short The mystery of the (not) missing coenzyme . . . . . . . . . . . . . . . . ?? Ben Short Sec and Tat share the workload . . . . . ?? Ben Short Tiam1 increases turnover . . . . . . . . ?? Ben Short Fyn regeneration . . . . . . . . . . . . 189 Caitlin Sedwick Laurie Boyer: Stem cell circuitry for commitment . . . . . . . . . . . . . . . 190 Scott X. Atwood and Anne Lynn S. Chang and Anthony E. Oro Hedgehog pathway inhibition and the race against tumor evolution . . . . . . . . 193 Tsukasa Okiyoneda and Gergely L. Lukacs Fixing cystic fibrosis by correcting CFTR domain assembly . . . . . . . . . . 199 Riekelt H. Houtkooper and Johan Auwerx Exploring the therapeutic space around NAD$^+$ . . . . . . . . . . . . . . . . 205 Mirna Perez-Moreno and Elaine Fuchs Guilt by association: What p120-catenin has to hide . . . . . . . . . . . . . . 211 Jérôme Lapointe and Ying Wang and Eve Bigras and Siegfried Hekimi The submitochondrial distribution of ubiquinone affects respiration in long-lived Mclk1$^{+/} - $ mice . . . . 215 Sa Kan Yoo and Christina M. Freisinger and Danny C. LeBert and Anna Huttenlocher Early redox, Src family kinase, and calcium signaling integrate wound responses and tissue regeneration in zebrafish . . . . . . . . . . . . . . . 225 Alex Pines and Mischa G. Vrouwe and Jurgen A. Marteijn and Dimitris Typas and Martijn S. Luijsterburg and Medine Cansoy and Paul Hensbergen and André Deelder and Anton de Groot and Syota Matsumoto and Kaoru Sugasawa and Nicolas Thoma and Wim Vermeulen and Harry Vrieling and Leon Mullenders PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1 . . . . . . . . . . 235 Fangwei Wang and Natalia P. Ulyanova and John R. Daum and Debasis Patnaik and Anna V. Kateneva and Gary J. Gorbsky and Jonathan M. G. Higgins Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation . . . . . . . . . . . . . . 251 Anna De Antoni and Stefano Maffini and Stefan Knapp and Andrea Musacchio and Stefano Santaguida A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B . . . . . . . . . . . . . . . . 269 Ana R. R. Maia and Zaira Garcia and Lilian Kabeche and Marin Barisic and Stefano Maffini and Sandra Macedo-Ribeiro and Iain M. Cheeseman and Duane A. Compton and Irina Kaverina and Helder Maiato Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore--microtubule attachments . . 285 Rebecca Keller and Jeanine de Keyzer and Arnold J. M. Driessen and Tracy Palmer Co-operation between different targeting pathways during integration of a membrane protein . . . . . . . . . . . . 303 Joseph P. Wynne and Jinhua Wu and Wenjuan Su and Adam Mor and Nikolaos Patsoukis and Vassiliki A. Boussiotis and Stevan R. Hubbard and Mark R. Philips Rap1-interacting adapter molecule (RIAM) associates with the plasma membrane via a proximity detector . . . . . . . . . . 317 Shujie Wang and Takashi Watanabe and Kenji Matsuzawa and Akira Katsumi and Mai Kakeno and Toshinori Matsui and Feng Ye and Kazuhide Sato and Kiyoko Murase and Ikuko Sugiyama and Kazushi Kimura and Akira Mizoguchi and Mark H. Ginsberg and John G. Collard and Kozo Kaibuchi Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration . . . . 331 Ivano Amelio and Anna Maria Lena and Giuditta Viticchi\`e and Ruby Shalom-Feuerstein and Alessandro Terrinoni and David Dinsdale and Giandomenico Russo and Claudia Fortunato and Elena Bonanno and Luigi Giusto Spagnoli and Daniel Aberdam and Richard Austen Knight and Eleonora Candi and Gerry Melino miR-24 triggers epidermal differentiation by controlling actin adhesion and cell migration . . . . . . 347 Benjamin A. Nanes and Christine Chiasson-MacKenzie and Anthony M. Lowery and Noboru Ishiyama and Victor Faundez and Mitsuhiko Ikura and Peter A. Vincent and Andrew P. Kowalczyk p120-catenin binding masks an endocytic signal conserved in classical cadherins 365 Iman Sahly and Eric Dufour and Cataldo Schietroma and Vincent Michel and Amel Bahloul and Isabelle Perfettini and Elise Pepermans and Amrit Estivalet and Diane Carette and Asadollah Aghaie and Inga Ebermann and Andrea Lelli and Maria Iribarne and Jean-Pierre Hardelin and Dominique Weil and José-Alain Sahel and Aziz El-Amraoui and Christine Petit Localization of Usher 1 proteins to the photoreceptor calyceal processes, which are absent from mice . . . . . . . . . . 381
Mitch Leslie HIV's kinesin chauffeur . . . . . . . . ?? Mitch Leslie Only skin deep, thanks to microtubules ?? Mitch Leslie N-WASP helps cancer cells open up . . . ?? Mitch Leslie Stay-at-home transcription factor saves axons . . . . . . . . . . . . . . . . . 403 Caitlin Sedwick Fumiyo Ikeda: Ubiquitin lines up for action . . . . . . . . . . . . . . . . . 405 Morten Lindow and Sakari Kauppinen Discovering the first microRNA-targeted drug . . . . . . . . . . . . . . . . . . 407 Lawrence Steinman The discovery of natalizumab, a potent therapeutic for multiple sclerosis . . . 413 Polakit Teekakirikul and Robert F. Padera and J. G. Seidman and Christine E. Seidman Hypertrophic cardiomyopathy: Translating cellular cross talk into therapeutics 417 David Boettiger Using force to visualize conformational activation of integrins . . . . . . . . 423 Bharat Joshi and Michele Bastiani and Scott S. Strugnell and Cecile Boscher and Robert G. Parton and Ivan R. Nabi Phosphocaveolin-1 is a mechanotransducer that induces caveola biogenesis via Egr1 transcriptional regulation . . . . . . . 425 Bhuvaneish Thangaraj Selvaraj and Nicolas Frank and Florian L. P. Bender and Esther Asan and Michael Sendtner Local axonal function of STAT3 rescues axon degeneration in the pmn model of motoneuron disease . . . . . . . . . . . 437 Chathurani S. Jayasena and Marianne E. Bronner Rbms3 functions in craniofacial development by posttranscriptionally modulating TGF-$ \beta $ signaling . . . 453 Raphaël Gaudin and Bruna Cunha de Alencar and Mabel Jouve and Stefano B\`erre and Emmanuel Le Bouder and Michael Schindler and Aditi Varthaman and François-Xavier Gobert and Philippe Benaroch Critical role for the kinesin KIF3A in the HIV life cycle in primary human macrophages . . . . . . . . . . . . . . 467 Emmanuel Dornier and Franck Coumailleau and Jean-François Ottavi and Julien Moretti and Claude Boucheix and Philippe Mauduit and François Schweisguth and Eric Rubinstein TspanC8 tetraspanins regulate ADAM10/Kuzbanian trafficking and promote Notch activation in flies and mammals 481 Wei Chen and Jizhong Lou and Evan A. Evans and Cheng Zhu Observing force-regulated conformational changes and ligand dissociation from a single integrin on cells . . . . . . . . 497 Kaelyn D. Sumigray and Henry P. Foote and Terry Lechler Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formation . . . . . 513 Xinzi Yu and Tobias Zech and Laura McDonald and Esther Garcia Gonzalez and Ang Li and Iain Macpherson and Juliane P. Schwarz and Heather Spence and Kinga Futó and Paul Timpson and Colin Nixon and Yafeng Ma and Ines M. Anton and Balázs Visegrády and Robert H. Insall and Karin Oien and Karen Blyth and Jim C. Norman and Laura M. Machesky N-WASP coordinates the delivery and F-actin-mediated capture of MT1-MMP at invasive pseudopods . . . . . . . . . . 527 Mei Rosa Ng and Achim Besser and Gaudenz Danuser and Joan S. Brugge Substrate stiffness regulates cadherin-dependent collective migration through myosin--II contractility . . . . 545
Ben Short ARL-13 SUMO wrestles receptors into cilia . . . . . . . . . . . . . . . . . ?? Ben Short Dnm1's recruitment strategy . . . . . . ?? Ben Short The fellowship of the Z ring . . . . . . ?? Ben Short Cdc42 helps cancer cells make their exit 567 Caitlin Sedwick Gaudenz Danuser: The symphony of the cell . . . . . . . . . . . . . . . . . . 568 Oliver Hantschel and Giulio Superti-Furga Cell biology: a key driver of therapeutic innovation . . . . . . . . . 571 Lawrence S. B. Goldstein New frontiers in human cell biology and medicine: Can pluripotent stem cells deliver? . . . . . . . . . . . . . . . . 577 Alfred L. Goldberg Development of proteasome inhibitors as research tools and cancer drugs . . . . 583 Yujie Li and Qing Zhang and Qing Wei and Yuxia Zhang and Kun Ling and Jinghua Hu SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors . . . . . . . . . . . . . . . 589 Astrid Klein and Lars Israel and Sebastian W. K. Lackey and Frank E. Nargang and Axel Imhof and Wolfgang Baumeister and Walter Neupert and Dennis R. Thomas Characterization of the insertase for $ \beta $-barrel proteins of the outer mitochondrial membrane . . . . . . . . . 599 Huyen T. Bui and Mary A. Karren and Debjani Bhar and Janet M. Shaw A novel motif in the yeast mitochondrial dynamin Dnm1 is essential for adaptor binding and membrane recruitment . . . . 613 Allan D. TerBush and Katherine W. Osteryoung Distinct functions of chloroplast FtsZ1 and FtsZ2 in Z-ring structure and remodeling . . . . . . . . . . . . . . . 623 Priyanka Sivadas and Jennifer M. Dienes and Martin St. Maurice and William D. Meek and Pinfen Yang A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex . . . . . . . . . . . . . . . . 639 Nicolas Reymond and Jae Hong Im and Ritu Garg and Francisco M. Vega and Barbara Borda d'Agua and Philippe Riou and Susan Cox and Ferran Valderrama and Ruth J. Muschel and Anne J. Ridley Cdc42 promotes transendothelial migration of cancer cells through $ \beta $1 integrin . . . . . . . . . . . 653 Matthew Raab and Joe Swift and P. C. Dave P. Dingal and Palak Shah and Jae-Won Shin and Dennis E. Discher Crawling from soft to stiff matrix polarizes the cytoskeleton and phosphoregulates myosin--II heavy chain 669 Arnab Sen and Zsanett Nagy-Zsvér-Vadas and Michael P. Krahn Drosophila PATJ supports adherens junction stability by modulating Myosin light chain activity . . . . . . . . . . 685 Jing Chen and Oxana E. Nekrasova and Dipal M. Patel and Jodi L. Klessner and Lisa M. Godsel and Jennifer L. Koetsier and Evangeline V. Amargo and Bhushan V. Desai and Kathleen J. Green The C-terminal unique region of desmoglein 2 inhibits its internalization via tail--tail interactions . . . . . . . . . . . . . . 699
Ben Short Why a microRNA's absence makes the heart grow larger . . . . . . . . . . . . . . ?? Ben Short A SIMPLE explanation for Charcot--Marie--Tooth disease . . . . . ?? Ben Short Global sourcing for ring construction ?? Ben Short KIF4 helps mitotic chromosomes get in shape . . . . . . . . . . . . . . . . . 715 Caitlin Sedwick Stefano Bertuzzi: Science first . . . . 716 Mar Carmena Flies stretch their cells to avoid a chromatin trap . . . . . . . . . . . . . 719 Frances M. Platt and Barry Boland and Aarnoud C. van der Spoel Lysosomal storage disorders: The cellular impact of lysosomal dysfunction 723 Haitong Hou and Zhou Zhou and Yu Wang and Jiyong Wang and Scott P. Kallgren and Tatiana Kurchuk and Elizabeth A. Miller and Fred Chang and Songtao Jia Csi1 links centromeres to the nuclear envelope for centromere clustering . . . 735 Shaila Kotadia and Emilie Montembault and William Sullivan and Anne Royou Cell elongation is an adaptive response for clearing long chromatid arms from the cleavage plane . . . . . . . . . . . 745 Kumiko Samejima and Itaru Samejima and Paola Vagnarelli and Hiromi Ogawa and Giulia Vargiu and David A. Kelly and Flavia de Lima Alves and Alastair Kerr and Lydia C. Green and Damien F. Hudson and Shinya Ohta and Carol A. Cooke and Christine J. Farr and Juri Rappsilber and William C. Earnshaw Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase II$ \alpha $ 755 Lisa Kappel and Mathias Loibl and Gertrude Zisser and Isabella Klein and Gernot Fruhmann and Christof Gruber and Stefan Unterweger and Gerald Rechberger and Brigitte Pertschy and Helmut Bergler Rlp24 activates the AAA--ATPase Drg1 to initiate cytoplasmic pre-60S maturation 771 Faye M. Drawnel and Dagmar Wachten and Jeffery D. Molkentin and Marjorie Maillet and Jan Magnus Aronsen and Fredrik Swift and Ivar Sjaastad and Ning Liu and Daniele Catalucci and Katsuhiko Mikoshiba and Chihiro Hisatsune and Hanneke Okkenhaug and Simon R. Andrews and Martin D. Bootman and H. Llewelyn Roderick Mutual antagonism between IP$_3$ RII and miRNA-133a regulates calcium signals and cardiac hypertrophy . . . . . . . . . . 783 Samuel M. Lee and Lih-Shen Chin and Lian Li Charcot-Marie-Tooth disease-linked protein SIMPLE functions with the ESCRT machinery in endosomal trafficking . . . 799 Sang-oh Han and Kunhong Xiao and Jihee Kim and Jiao-Hui Wu and James W. Wisler and Nobuhiro Nakamura and Neil J. Freedman and Sudha K. Shenoy MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound $ \beta_2 $-adrenergic receptors . . . . . 817 Junqi Huang and Yinyi Huang and Haochen Yu and Dhivya Subramanian and Anup Padmanabhan and Rahul Thadani and Yaqiong Tao and Xie Tang and Roland Wedlich-Soldner and Mohan K. Balasubramanian Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast . . . . . . . . . . . . . 831 Wenjing Li and Takashi Moriwaki and Tomomi Tani and Takashi Watanabe and Kozo Kaibuchi and Gohta Goshima Reconstitution of dynamic microtubules with \bionameDrosophila XMAP215, EB1, and Sentin . . . . . . . . . . . . . . . 849 Nia J. Bryant and David E. James The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport . . . . . . . . . . . 863
Ben Short Release notes for dense-core vesicles ?? Ben Short Opening up the ER's gatekeeper . . . . . ?? Ben Short Segregating Bub1's mitotic functions . . ?? Ben Short Farp1 gives both sides of the story . . 867 Caitlin Sedwick John Heuser: Capture the moment . . . . 868 Neil J. Ganem and David Pellman Linking abnormal mitosis to the acquisition of DNA damage . . . . . . . 871 Rhea van de Bospoort and Margherita Farina and Sabine K. Schmitz and Arthur de Jong and Heidi de Wit and Matthijs Verhage and Ruud F. Toonen Munc13 controls the location and efficiency of dense-core vesicle release in neurons . . . . . . . . . . . . . . . 883 Dimitri Conomos and Michael D. Stutz and Mark Hills and Axel A. Neumann and Tracy M. Bryan and Roger R. Reddel and Hilda A. Pickett Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells . . . . . 893 Steven F. Trueman and Elisabet C. Mandon and Reid Gilmore A gating motif in the translocation channel sets the hydrophobicity threshold for signal sequence function 907 Sarah Whitehouse and Vicki A. M. Gold and Alice Robson and William J. Allen and Richard B. Sessions and Ian Collinson Mobility of the SecA $2$-helix-finger is not essential for polypeptide translocation via the SecYEG complex . . 919 Robin M. Ricke and Karthik B. Jeganathan and Liviu Malureanu and Andrew M. Harrison and Jan M. van Deursen Bub1 kinase activity drives error correction and mitotic checkpoint control but not tumor suppression . . . 931 Syed M. Ahmed and Brigitte L. Thériault and Maruti Uppalapati and Catherine W. N. Chiu and Brenda L. Gallie and Sachdev S. Sidhu and Stéphane Angers KIF14 negatively regulates Rap1a--Radil signaling during breast cancer progression . . . . . . . . . . . . . . 951 Raghuvir Viswanatha and Patrice Y. Ohouo and Marcus B. Smolka and Anthony Bretscher Local phosphocycling mediated by LOK/SLK restricts ezrin function to the apical aspect of epithelial cells . . . . . . . 969 Lucas Cheadle and Thomas Biederer The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization . . . . . 985 Fubito Nakatsu and Jeremy M. Baskin and Jeeyun Chung and Lukas B. Tanner and Guanghou Shui and Sang Yoon Lee and Michelle Pirruccello and Mingming Hao and Nicholas T. Ingolia and Markus R. Wenk and Pietro De Camilli PtdIns4P synthesis by PI4KIII$ \alpha $ at the plasma membrane and its impact on plasma membrane identity . . . . . . . . 1003
Ben Short Apoptotic regulators dispose of the midbody . . . . . . . . . . . . . . . . ?? Ben Short Secretory proteins hail a cab at the TGN ?? Ben Short How endothelial junctions get deep--Syx'd . . . . . . . . . . . . . . ?? Ben Short Lkb1 takes different paths to morphogenesis . . . . . . . . . . . . . 1019 Caitlin Sedwick Li-Huei Tsai: I well remember . . . . . 1020 Liang Cai and Keith E. Mostov Cell height: Tao rising . . . . . . . . 1023 Anna Noatynska and Monica Gotta and Patrick Meraldi Mitotic spindle (DIS)orientation and DISease: Cause or consequence? . . . . . 1025 Hervé Menoni and Jan H. J. Hoeijmakers and Wim Vermeulen Nucleotide excision repair--initiating proteins bind to oxidative DNA lesions in vivo . . . . . . . . . . . . . . . . 1037 Yongping Chai and Dong Tian and Yihong Yang and Guoxin Feng and Ze Cheng and Wei Li and Guangshuo Ou Apoptotic regulators promote cytokinetic midbody degradation in \bionameC. elegans . . . . . . . . . . . . . . . . 1047 Julia von Blume and Anne-Marie Alleaume and Christine Kienzle and Amado Carreras-Sureda and Miguel Valverde and Vivek Malhotra Cab45 is required for Ca$^{2+}$-dependent secretory cargo sorting at the trans-Golgi network . . . 1057 Céline Courilleau and Catherine Chailleux and Alain Jauneau and Fanny Grimal and Sébastien Briois and Elisa Boutet-Robinet and François Boudsocq and Didier Trouche and Yvan Canitrot The chromatin remodeler p400 ATPase facilitates Rad51-mediated repair of DNA double-strand breaks . . . . . . . . . . 1067 Kerstin N. Schmidt and Stefanie Kuhns and Annett Neuner and Birgit Hub and Hanswalter Zentgraf and Gislene Pereira Cep164 mediates vesicular docking to the mother centriole during early steps of ciliogenesis . . . . . . . . . . . . . . 1083 Siu P. Ngok and Rory Geyer and Miaoliang Liu and Antonis Kourtidis and Sudesh Agrawal and Chuanshen Wu and Himabindu Reddy Seerapu and Laura J. Lewis-Tuffin and Karen L. Moodie and Deborah Huveldt and Ruth Marx and Jay M. Baraban and Peter Storz and Arie Horowitz and Panos Z. Anastasiadis VEGF and Angiopoietin-1 exert opposing effects on cell junctions by regulating the Rho GEF Syx . . . . . . . . . . . . 1103 Bryan Lo and Geraldine Strasser and Meredith Sagolla and Cary D. Austin and Melissa Junttila and Ira Mellman Lkb1 regulates organogenesis and early oncogenesis along AMPK-dependent and -independent pathways . . . . . . . . . 1117 Juan Manuel Gomez and Ying Wang and Veit Riechmann Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis . . . 1131 Hyung Joon Kim and Vikram Prasad and Seok-Won Hyung and Zang Hee Lee and Sang-Won Lee and Aditi Bhargava and David Pearce and Youngkyun Lee and Hong-Hee Kim Plasma membrane calcium ATPase regulates bone mass by fine-tuning osteoclast differentiation and survival . . . . . . 1145
Mitch Leslie Kinetochore complexes need teamwork . . ?? Mitch Leslie Blame ATAD5 for factory closures . . . . ?? Mitch Leslie Cdc42 helps cells take bigger bites . . ?? Mitch Leslie The two stages of cell fusion . . . . . 3 Caitlin Sedwick Gillian Griffiths: How T cells get on target . . . . . . . . . . . . . . . . . 4 Ana Stankovic and Lars E. T. Jansen Reductionism at the vertebrate kinetochore . . . . . . . . . . . . . . 7 Keith Burridge and Erika S. Wittchen The tension mounts: Stress fibers as force-generating mechanotransducers . . 9 Fabienne Lampert and Christine Mieck and Gregory M. Alushin and Eva Nogales and Stefan Westermann Molecular requirements for the formation of a kinetochore--microtubule interface by Dam1 and Ndc80 complexes . . . . . . 21 Kyoo-young Lee and Haiqing Fu and Mirit I. Aladjem and Kyungjae Myung ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin . . . . . . . . . 31 Tetsuya Hori and Wei-Hao Shang and Kozo Takeuchi and Tatsuo Fukagawa The CCAN recruits CENP--A to the centromere and forms the structural core for kinetochore assembly . . . . . . . . 45 Ramya Nair and Juliane Lauks and SangYong Jung and Nancy E. Cooke and Heidi de Wit and Nils Brose and Manfred W. Kilimann and Matthijs Verhage and JeongSeop Rhee Neurobeachin regulates neurotransmitter receptor trafficking to synapses . . . . 61 Sina Mohammadi and Ralph R. Isberg Cdc42 interacts with the exocyst complex to promote phagocytosis . . . . . . . . 81 Maryna Kapustina and Timothy C. Elston and Ken Jacobson Compression and dilation of the membrane-cortex layer generates rapid changes in cell shape . . . . . . . . . 95 Evgenia Leikina and Kamran Melikov and Sarmistha Sanyal and Santosh K. Verma and Bokkee Eun and Claudia Gebert and Karl Pfeifer and Vladimir A. Lizunov and Michael M. Kozlov and Leonid V. Chernomordik Extracellular annexins and dynamin are important for sequential steps in myoblast fusion . . . . . . . . . . . . 109
Mitch Leslie Ran's downhill slide during mitosis . . ?? Mitch Leslie Fly chromosomes don't just ride the tips ?? Mitch Leslie Spreading around a Wnt protein . . . . . ?? Mitch Leslie How to live without BRCA1 . . . . . . . 127 Caitlin Sedwick Samara Reck--Peterson: Dynein defies expectations . . . . . . . . . . . . . . 128 Francis J. McNally Mechanisms of spindle positioning . . . 131 Yeon-Tae Jeong and Mario Rossi and Lukas Cermak and Anita Saraf and Laurence Florens and Michael P. Washburn and Patrick Sung and Carl L. Schildkraut and Michele Pagano FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress . . . . . . . . . . . 141 Keisuke Hasegawa and Sung Jin Ryu and Petr Kaláb Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells . . . . . . . 151 Michael Lazarou and Derek P. Narendra and Seok Min Jin and Ephrem Tekle and Soojay Banerjee and Richard J. Youle PINK1 drives Parkin self-association and HECT-like E3 activity upstream of mitochondrial binding . . . . . . . . . 163 Jessica Cottarel and Philippe Frit and Oriane Bombarde and Bernard Salles and Aurélie Négrel and Stéphanie Bernard and Penny A. Jeggo and Michael R. Lieber and Mauro Modesti and Patrick Calsou A noncatalytic function of the ligation complex during nonhomologous end joining 173 David A. Grotsky and Ignacio Gonzalez-Suarez and Anna Novell and Martin A. Neumann and Sree C. Yaddanapudi and Monica Croke and Montserrat Martinez-Alonso and Abena B. Redwood and Sylvia Ortega-Martinez and Zhihui Feng and Enrique Lerma and Teresa Ramon y Cajal and Junran Zhang and Xavier Matias-Guiu and Adriana Dusso and Susana Gonzalo BRCA1 loss activates cathepsin L-mediated degradation of 53BP1 in breast cancer cells . . . . . . . . . . 187 Stuart Cane and Anna A. Ye and Sasha J. Luks-Morgan and Thomas J. Maresca Elevated polar ejection forces stabilize kinetochore--microtubule attachments . . 203 Keisuke Kamimura and Kohei Ueno and Jun Nakagawa and Rie Hamada and Minoru Saitoe and Nobuaki Maeda Perlecan regulates bidirectional Wnt signaling at the \bionameDrosophila neuromuscular junction . . . . . . . . . 219
Ben Short Working at the tail end of tubulin . . . ?? Ben Short GEFs help Rabs find their target . . . . ?? Ben Short Chemokine turnover keeps neurons on track . . . . . . . . . . . . . . . . . ?? Ben Short MDGAs help the brain lose its inhibitions . . . . . . . . . . . . . . 237 Caitlin Sedwick Carl-Philipp Heisenberg: Early embryos make a big move . . . . . . . . . . . . 238 Natasha T. Snider and Jessica M. Leonard and Raymond Kwan and Nicholas W. Griggs and Liangyou Rui and M. Bishr Omary Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation . . . . . . . . . . . . . . 241 Yasuhisa Sakamoto and Batiste Boëda and Sandrine Etienne-Manneville APC binds intermediate filaments and is required for their reorganization during cell migration . . . . . . . . . . . . . 249 Andrea E. Prota and Maria M. Magiera and Marijn Kuijpers and Katja Bargsten and Daniel Frey and Mara Wieser and Rolf Jaussi and Casper C. Hoogenraad and Richard A. Kammerer and Carsten Janke and Michel O. Steinmetz Structural basis of tubulin tyrosination by tubulin tyrosine ligase . . . . . . . 259 Carsten Wloka and Elizabeth A. Vallen and Lydia Thé and Xiaodong Fang and Younghoon Oh and Erfei Bi Immobile myosin--II plays a scaffolding role during cytokinesis in budding yeast 271 Julia Blümer and Juliana Rey and Leif Dehmelt and Tomá\vs Mazel and Yao-Wen Wu and Philippe Bastiaens and Roger S. Goody and Aymelt Itzen RabGEFs are a major determinant for specific Rab membrane targeting . . . . 287 Vanesa M. Tomatis and Andreas Papadopulos and Nancy T. Malintan and Sally Martin and Tristan Wallis and Rachel S. Gormal and John Kendrick-Jones and Folma Buss and Frédéric A. Meunier Myosin VI small insert isoform maintains exocytosis by tethering secretory granules to the cortical actin . . . . . 301 Katherine L. Pettem and Daisaku Yokomaku and Hideto Takahashi and Yuan Ge and Ann Marie Craig Interaction between autism-linked MDGAs and neuroligins suppresses inhibitory synapse development . . . . . . . . . . 321 Stephen W. Lewellis and Danielle Nagelberg and Abhi Subedi and Alison Staton and Michelle LeBlanc and Antonio Giraldez and Holger Knaut Precise SDF1-mediated cell guidance is achieved through ligand clearance and microRNA-mediated decay . . . . . . . . 337 Yujie Li and Qing Zhang and Qing Wei and Yuxia Zhang and Kun Ling and Jinghua Hu SUMOylation of the small GTPase ARL-13 promotes ciliary targeting of sensory receptors . . . . . . . . . . . . . . . 357 Addolarata Pisconti and Silvia Brunelli and Monica Di Padova and Clara De Palma and Daniela Deponti and Silvia Baesso and Vittorio Sartorelli and Giulio Cossu and Emilio Clementi Follistatin induction by nitric oxide through cyclic GMP: a tightly regulated signaling pathway that controls myoblast fusion . . . . . . . . . . . . . . . . . 359
Mitch Leslie Telocentrosomes organize telomere gathering . . . . . . . . . . . . . . . ?? Mitch Leslie SecYEG gets grabby with new proteins . . ?? Mitch Leslie Condensin II helps sisters find their identity . . . . . . . . . . . . . . . . ?? Mitch Leslie Endocytosis puts exocytosis in pole position . . . . . . . . . . . . . . . . 363 Caitlin Sedwick Stephen Doxsey: At the (peri)center of things . . . . . . . . . . . . . . . . . 364 Clifford V. Harding and John E. Heuser and Philip D. Stahl Exosomes: Looking back three decades and into the future . . . . . . . . . . . . 367 Graça Raposo and Willem Stoorvogel Extracellular vesicles: Exosomes, microvesicles, and friends . . . . . . . 373 Masashi Yoshida and Satoshi Katsuyama and Kazuki Tateho and Hiroto Nakamura and Junpei Miyoshi and Tatsunori Ohba and Hirotada Matsuhara and Futaba Miki and Koei Okazaki and Tokuko Haraguchi and Osami Niwa and Yasushi Hiraoka and Ayumu Yamamoto Microtubule-organizing center formation at telomeres induces meiotic telomere clustering . . . . . . . . . . . . . . . 385 David Akopian and Kush Dalal and Kuang Shen and Franck Duong and Shu-ou Shan SecYEG activates GTPases to drive the completion of cotranslational protein targeting . . . . . . . . . . . . . . . 397 Mini Jose and Sylvain Tollis and Deepak Nair and Jean-Baptiste Sibarita and Derek McCusker Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism . . . . . . . . . . . . . . . 407 Ingyu Kim and Weijun Pan and Sara A. Jones and Youxin Zhang and Xiaowei Zhuang and Dianqing Wu Clathrin and AP2 are required for PtdIns(4,5)P$_2$-mediated formation of LRP6 signalosomes . . . . . . . . . . . 419 Takao Ono and Daisuke Yamashita and Tatsuya Hirano Condensin II initiates sister chromatid resolution during S phase . . . . . . . 429 Kee K. Kim and Joseph Nam and Yoh-suke Mukouyama and Sachiyo Kawamoto Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development . . . . . . . . . . . 443 J. Richard McIntosh and Eileen O'Toole and Kirill Zhudenkov and Mary Morphew and Cindi Schwartz and Fazly I. Ataullakhanov and Ekaterina L. Grishchuk Conserved and divergent features of kinetochores and spindle microtubule ends from five species . . . . . . . . . 459 Jose A. Martina and Rosa Puertollano Rag GTPases mediate amino acid-dependent recruitment of TFEB and MITF to lysosomes . . . . . . . . . . . . . . . 475 Zamal Ahmed and Chi-Chuan Lin and Kin M. Suen and Fernando A. Melo and James A. Levitt and Klaus Suhling and John E. Ladbury Grb2 controls phosphorylation of FGFR2 by inhibiting receptor kinase and Shp2 phosphatase activity . . . . . . . . . . 493 Stefanie Kuhns and Kerstin N. Schmidt and Jürgen Reymann and Daniel F. Gilbert and Annett Neuner and Birgit Hub and Ricardo Carvalho and Philipp Wiedemann and Hanswalter Zentgraf and Holger Erfle and Ursula Klingmüller and Michael Boutros and Gislene Pereira The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis . . . . . . . 505 Davide Randazzo and Emiliana Giacomello and Stefania Lorenzini and Daniela Rossi and Enrico Pierantozzi and Bert Blaauw and Carlo Reggiani and Stephan Lange and Angela K. Peter and Ju Chen and Vincenzo Sorrentino Obscurin is required for ankyrinB-dependent dystrophin localization and sarcolemma integrity 523 Joachim Albers and Johannes Keller and Anke Baranowsky and Frank Timo Beil and Philip Catala-Lehnen and Jochen Schulze and Michael Amling and Thorsten Schinke Canonical Wnt signaling inhibits osteoclastogenesis independent of osteoprotegerin . . . . . . . . . . . . 537
Ben Short Heat shock gene resides in a pore neighborhood . . . . . . . . . . . . . . ?? Ben Short Breaking down Arp2/3 function . . . . . ?? Ben Short PEX26 takes the direct route . . . . . . ?? Ben Short Notochord vacuoles make a rod for the vertebrate back . . . . . . . . . . . . 553 Caitlin Sedwick Martin Humphries: Attached to adhesion 554 Florencia Rago and Iain M. Cheeseman The functions and consequences of force at kinetochores . . . . . . . . . . . . 557 Kexi Yi and Boris Rubinstein and Jay R. Unruh and Fengli Guo and Brian D. Slaughter and Rong Li Sequential actin-based pushing forces drive meiosis I chromosome migration and symmetry breaking in oocytes . . . . . . 567 Katherine E. Sloan and Sandy Mattijssen and Simon Lebaron and David Tollervey and Ger J. M. Pruijn and Nicholas J. Watkins Both endonucleolytic and exonucleolytic cleavage mediate ITS1 removal during human ribosomal RNA processing . . . . . 577 Sabine Rohner and Veronique Kalck and Xuefei Wang and Kohta Ikegami and Jason D. Lieb and Susan M. Gasser and Peter Meister Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in \bionameCaenorhabditis elegans . . . . . 589 Oliver Dreesen and Alexandre Chojnowski and Peh Fern Ong and Tian Yun Zhao and John E. Common and Declan Lunny and E. Birgitte Lane and Shu Jin Lee and Leah A. Vardy and Colin L. Stewart and Alan Colman Lamin B1 fluctuations have differential effects on cellular proliferation and senescence . . . . . . . . . . . . . . . 605 Elena Ingerman and Jennifer Ying Hsiao and R. Dyche Mullins Arp2\slash 3 complex ATP hydrolysis promotes lamellipodial actin network disassembly but is dispensable for assembly . . . . . . . . . . . . . . . . 619 Junya Hayase and Sachiko Kamakura and Yuko Iwakiri and Yoshihiro Yamaguchi and Tomoko Izaki and Takashi Ito and Hideki Sumimoto The WD40 protein Morg1 facilitates Par6--aPKC binding to Crb3 for apical identity in epithelial cells . . . . . . 635 Yuichi Yagita and Takahide Hiromasa and Yukio Fujiki Tail-anchored PEX26 targets peroxisomes via a PEX19-dependent and TRC40-independent class I pathway . . . 651 Kathryn Ellis and Jennifer Bagwell and Michel Bagnat Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis . . . . . . . . . . 667 Bharat Joshi and Michele Bastiani and Scott S. Strugnell and Cecile Boscher and Robert G. Parton and Ivan R. Nabi Phosphocaveolin-1 is a mechanotransducer that induces caveola biogenesis via Egr1 transcriptional regulation . . . . . . . 681
Ben Short Oncogenes induce a reversal of replication's fortunes . . . . . . . . . ?? Ben Short Misshapen orders an egg roll . . . . . . ?? Ben Short CUL4B keeps replication firing on all cylinders . . . . . . . . . . . . . . . ?? Ben Short The plastic proteome . . . . . . . . . . 685 Caitlin Sedwick Jürgen Roth: Immunogold master . . . . . 686 Romain Levayer and Eduardo Moreno Mechanisms of cell competition: Themes and variations . . . . . . . . . . . . . 689 Kai J. Neelsen and Isabella M. Y. Zanini and Raquel Herrador and Massimo Lopes Oncogenes induce genotoxic stress by mitotic processing of unusual replication intermediates . . . . . . . 699 Nathalie Jurisch-Yaksi and Applonia J. Rose and Huiqi Lu and Tim Raemaekers and Sebastian Munck and Pieter Baatsen and Veerle Baert and Wendy Vermeire and Suzie J. Scales and Daphne Verleyen and Roel Vandepoel and Przemko Tylzanowski and Emre Yaksi and Thomy de Ravel and H. Joseph Yost and Guy Froyen and Cammon B. Arrington and Wim Annaert Rer1p maintains ciliary length and signaling by regulating $ \gamma $-secretase activity and Foxj1a levels 709 Lindsay Lewellyn and Maureen Cetera and Sally Horne-Badovinac Misshapen decreases integrin levels to promote epithelial motility and planar polarity in \bionameDrosophila . . . . . 721 Hongyu Zhao and Yan G. Zhao and Xingwei Wang and Lanjun Xu and Lin Miao and Du Feng and Quan Chen and Attila L. Kovács and Dongsheng Fan and Hong Zhang Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration 731 Yongxin Zou and Jun Mi and Wenxing Wang and Juanjuan Lu and Wei Zhao and Zhaojian Liu and Huili Hu and Yang Yang and Xiaoxing Gao and Baichun Jiang and Changshun Shao and Yaoqin Gong CUL4B promotes replication licensing by up-regulating the CDK2--CDC6 cascade . . 743 Andrew D. Stephens and Rachel A. Haggerty and Paula A. Vasquez and Leandra Vicci and Chloe E. Snider and Fu Shi and Cory Quammen and Christopher Mullins and Julian Haase and Russell M. Taylor and Jolien S. Verdaasdonk and Michael R. Falvo and Yuan Jin and M. Gregory Forest and Kerry Bloom Pericentric chromatin loops function as a nonlinear spring in mitotic force balance . . . . . . . . . . . . . . . . 757 Mei Zhu and Florian Settele and Sachin Kotak and Luis Sanchez-Pulido and Lena Ehret and Chris P. Ponting and Pierre Gönczy and Ingrid Hoffmann MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression . . . . . . . . . . 773 Anthony J. Morgan and Lianne C. Davis and Siegfried K. T. Y. Wagner and Alexander M. Lewis and John Parrington and Grant C. Churchill and Antony Galione Bidirectional Ca$^{2+}$ signaling occurs between the endoplasmic reticulum and acidic organelles . . . . . . . . . . . 789 Ke Zhang and Zhihong Li and Manish Jaiswal and Vafa Bayat and Bo Xiong and Hector Sandoval and Wu-Lin Charng and Gabriela David and Claire Haueter and Shinya Yamamoto and Brett H. Graham and Hugo J. Bellen The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit . . . . . . . . . . . 807 Wen-Fang Xia and Fu-Lei Tang and Lei Xiong and Shan Xiong and Ji-Ung Jung and Dae-Hoon Lee and Xing-Sheng Li and Xu Feng and Lin Mei and Wen-Cheng Xiong Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via sustained RANKL signaling . . . . . . . 821 Michal Breker and Melissa Gymrek and Maya Schuldiner A novel single-cell screening platform reveals proteome plasticity during yeast stress responses . . . . . . . . . . . . 839 Daniele Catalucci and Deng-Hong Zhang and Jaime DeSantiago and Franck Aimond and Guillaume Barbara and Jean Chemin and Désiré Bonci and Eckard Picht and Francesca Rusconi and Nancy D. Dalton and Kirk L. Peterson and Sylvain Richard and Donald M. Bers and Joan Heller Brown and Gianluigi Condorelli Akt regulates L-type Ca$^{2+}$ channel activity by modulating Ca$_v$ $ \alpha $1 protein stability . . . . . . . . . . 851 Arnab Sen and Zsanett Nagy-Zsvér-Vadas and Michael P. Krahn Drosophila PATJ supports adherens junction stability by modulating Myosin light chain activity . . . . . . . . . . 853
Mitch Leslie FANCJ lets chromatin stay true . . . . . ?? Mitch Leslie STT3B gets the tip . . . . . . . . . . . ?? Mitch Leslie Actin draws a cadherin crowd . . . . . . ?? Mitch Leslie Rise and fall of the kinetochore . . . . 3 Caitlin Sedwick Sandhya Koushika: Building new models and communities . . . . . . . . . . . . 4 Mike Rossner New mandates? No problem for The Rockefeller University Press . . . . . . 7 Robin M. Ricke and Jan M. van Deursen Aneuploidy in health, disease, and aging 11 Karen E. Gascoigne and Iain M. Cheeseman CDK-dependent phosphorylation and nuclear exclusion coordinately control kinetochore assembly state . . . . . . . 23 Rebekka A. Schwab and Jadwiga Nieminuszczy and Kazuo Shin-ya and Wojciech Niedzwiedz FANCJ couples replication past natural fork barriers with maintenance of chromatin structure . . . . . . . . . . 33 Daniel W. Buster and Scott G. Daniel and Huy Q. Nguyen and Sarah L. Windler and Lara C. Skwarek and Maureen Peterson and Meredith Roberts and Joy H. Meserve and Tom Hartl and Joseph E. Klebba and David Bilder and Giovanni Bosco and Gregory C. Rogers SCF$^{\rm Slimb}$ ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap--H2 . . 49 David Reboutier and Marie-Béreng\`ere Troadec and Jean-Yves Cremet and Lucie Chauvin and Vincent Guen and Patrick Salaun and Claude Prigent Aurora A is involved in central spindle assembly through phosphorylation of Ser 19 in P150Glued . . . . . . . . . . . . 65 Shiteshu Shrimal and Steven F. Trueman and Reid Gilmore Extreme C-terminal sites are posttranslocationally glycosylated by the STT3B isoform of the OST . . . . . . 81 Arun T. John Peter and Jens Lachmann and Meenakshi Rana and Madeleine Bunge and Margarita Cabrera and Christian Ungermann The BLOC-1 complex promotes endosomal maturation by recruiting the Rab5 GTPase-activating protein Msb3 . . . . . 97 Long Lin and Peiguo Yang and Xinxin Huang and Hui Zhang and Qun Lu and Hong Zhang The scaffold protein EPG-7 links cargo--receptor complexes with the autophagic assembly machinery . . . . . 113 Soonjin Hong and Regina B. Troyanovsky and Sergey M. Troyanovsky Binding to F-actin guides cadherin cluster assembly, stability, and movement . . . . . . . . . . . . . . . . 131 Tharan Srikumar and Megan C. Lewicki and Michael Costanzo and Johnny M. Tkach and Harm van Bakel and Kyle Tsui and Erica S. Johnson and Grant W. Brown and Brenda J. Andrews and Charles Boone and Guri Giaever and Corey Nislow and Brian Raught Global analysis of SUMO chain function reveals multiple roles in chromatin regulation . . . . . . . . . . . . . . . 145 Yanmei Qi and Xiaoxiang Tian and Jie Liu and Yaling Han and Alan M. Graham and M. Celeste Simon and Josef M. Penninger and Peter Carmeliet and Shaohua Li Bnip3 and AIF cooperate to induce apoptosis and cavitation during epithelial morphogenesis . . . . . . . . 165 Mikito Takefuji and Marcus Krüger and Kishor K. Sivaraj and Kozo Kaibuchi and Stefan Offermanns and Nina Wettschureck RhoGEF12 controls cardiac remodeling by integrating G protein-- and integrin-dependent signaling cascades ?? Inge Van de Walle and Els Waegemans and Jelle De Medts and Greet De Smet and Magda De Smedt and Sylvia Snauwaert and Bart Vandekerckhove and Tessa Kerre and Georges Leclercq and Jean Plum and Thomas Gridley and Tao Wang and Ute Koch and Freddy Radtke and Tom Taghon Specific Notch receptor--ligand interactions control human TCR-ab/gd development by inducing differential Notch signal strength . . . . . . . . . ??
Ben Short Frog oocytes evade the checkpoint . . . ?? Ben Short The BBSome's export duty . . . . . . . . ?? Ben Short Hypoxia takes invadopodia up a Notch . . ?? Ben Short Setting an immuno--TRAP for PML nuclear bodies . . . . . . . . . . . . . . . . . 169 Caitlin Sedwick Margaret Gardel: At the interface of physics and biology . . . . . . . . . . 170 Stephen M. King A solid-state control system for dynein-based ciliary/flagellar motility 173 Ivana Primorac and Andrea Musacchio \bionamePanta rhei: The APC/C at steady state . . . . . . . . . . . . . . . . . 177 Hua Shao and Ruizhen Li and Chunqi Ma and Eric Chen and X. Johné Liu Xenopus oocyte meiosis lacks spindle assembly checkpoint control . . . . . . 191 Jonne A. Raaijmakers and Marvin E. Tanenbaum and René H. Medema Systematic dissection of dynein regulators in mitosis . . . . . . . . . 201 Wilco Nijenhuis and Eleonore von Castelmur and Dene Littler and Valeria De Marco and Eelco Tromer and Mathijs Vleugel and Maria H. J. van Osch and Berend Snel and Anastassis Perrakis and Geert J. P. L. Kops A TPR domain--containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B . . 217 Lymarie Maldonado-Báez and Nelson B. Cole and Helmut Krämer and Julie G. Donaldson Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1 233 Karl F. Lechtreck and Jason M. Brown and Julio L. Sampaio and Julie M. Craft and Andrej Shevchenko and James E. Evans and George B. Witman Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase . . 249 Ryosuke Yamamoto and Kangkang Song and Haru-aki Yanagisawa and Laura Fox and Toshiki Yagi and Maureen Wirschell and Masafumi Hirono and Ritsu Kamiya and Daniela Nicastro and Winfield S. Sale The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility . . . . . . . . . . . . 263 Begoña Díaz and Angela Yuen and Shinji Iizuka and Shigeki Higashiyama and Sara A. Courtneidge Notch increases the shedding of HB--EGF by ADAM12 to potentiate invadopodia formation in hypoxia . . . . . . . . . . 279 Venus Thomanetz and Nico Angliker and Dimitri Cloëtta and Regula M. Lustenberger and Manuel Schweighauser and Filippo Oliveri and Noboru Suzuki and Markus A. Rüegg Ablation of the mTORC2 component rictor in brain or Purkinje cells affects size and neuron morphology . . . . . . . . . 293 Anthony I. Romer and Jagmohan Singh and Satish Rattan and Robert S. Krauss Smooth muscle fascicular reorientation is required for esophageal morphogenesis and dependent on Cdo . . . . . . . . . . 309 Reagan W. Ching and Kashif Ahmed and Paul C. Boutros and Linda Z. Penn and David P. Bazett-Jones Identifying gene locus associations with promyelocytic leukemia nuclear bodies using immuno--TRAP . . . . . . . . . . . 325 Edward Avezov and Benedict C. S. Cross and Gabriele S. Kaminski Schierle and Mikael Winters and Heather P. Harding and Eduardo Pinho Melo and Clemens F. Kaminski and David Ron Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox . . . . . . 337 Mitch Leslie FANCJ lets chromatin stay true . . . . . 351 Michal Breker and Melissa Gymrek and Maya Schuldiner A novel single-cell screening platform reveals proteome plasticity during yeast stress responses . . . . . . . . . . . . 353
Ben Short Fewer forks make faster progress . . . . ?? Ben Short Bub3's function doesn't stop at the checkpoint . . . . . . . . . . . . . . . ?? Ben Short EphA2 cleavage helps tumor cells go it alone . . . . . . . . . . . . . . . . . ?? Ben Short Sanpodo seals precursors' fate . . . . . 357 Caitlin Sedwick Carla Koehler: Small TIMs are a big deal 358 Yun R. Li and Oliver D. King and James Shorter and Aaron D. Gitler Stress granules as crucibles of ALS pathogenesis . . . . . . . . . . . . . . 361 Yuan Zhong and Tittu Nellimoottil and Jared M. Peace and Simon R. V. Knott and Sandra K. Villwock and Janis M. Yee and Jeffrey M. Jancuska and Sanket Rege and Marianne Tecklenburg and Robert A. Sclafani and Simon Tavaré and Oscar M. Aparicio The level of origin firing inversely affects the rate of replication fork progression . . . . . . . . . . . . . . 373 Sara Morais da Silva and Tatiana Moutinho-Santos and Claudio E. Sunkel A tumor suppressor role of the Bub3 spindle checkpoint protein after apoptosis inhibition . . . . . . . . . . 385 Valérie Bergoglio and Anne-Sophie Boyer and Erin Walsh and Valeria Naim and Gaëlle Legube and Marietta Y. W. T. Lee and Laurie Rey and Filippo Rosselli and Christophe Cazaux and Kristin A. Eckert and Jean-Sébastien Hoffmann DNA synthesis by Pol $ \eta $ promotes fragile site stability by preventing under-replicated DNA in mitosis . . . . 395 An Zeng and Yong-Qin Li and Chen Wang and Xiao-Shuai Han and Ge Li and Jian-Yong Wang and Dang-Sheng Li and Yong-Wen Qin and Yufang Shi and Gary Brewer and Qing Jing Heterochromatin protein 1 promotes self-renewal and triggers regenerative proliferation in adult stem cells . . . 409 Amelia U. Contreras and Yohannes Mebratu and Monica Delgado and Gilbert Montano and Chien-an A. Hu and Stefan W. Ryter and Augustine M. K. Choi and Yuting Lin and Jialing Xiang and Hitendra Chand and Yohannes Tesfaigzi Deacetylation of p53 induces autophagy by suppressing Bmf expression . . . . . 427 Alok Upadhyay and Vasundhara Kandachar and Diana Zitserman and Xin Tong and Fabrice Roegiers Sanpodo controls sensory organ precursor fate by directing Notch trafficking and binding $ \gamma $-secretase . . . . . . 439 Roberta Martinelli and Masataka Kamei and Peter T. Sage and Ramiro Massol and Laya Varghese and Tracey Sciuto and Mourad Toporsian and Ann M. Dvorak and Tomas Kirchhausen and Timothy A. Springer and Christopher V. Carman Release of cellular tension signals self-restorative ventral lamellipodia to heal barrier micro-wounds . . . . . . . 449 Nami Sugiyama and Erika Gucciardo and Olga Tatti and Markku Varjosalo and Marko Hyytiäinen and Matthias Gstaiger and Kaisa Lehti EphA2 cleavage by MT1-MMP triggers single cancer cell invasion via homotypic cell repulsion . . . . . . . . 467 Clifford V. Harding and John E. Heuser and Philip D. Stahl Exosomes: Looking back three decades and into the future . . . . . . . . . . . . 485 David Reboutier and Marie-Béreng\`ere Troadec and Jean-Yves Cremet and Lucie Chauvin and Vincent Guen and Patrick Salaun and Claude Prigent Aurora A is involved in central spindle assembly through phosphorylation of Ser 19 in P150Glued . . . . . . . . . . . . 487
Mitch Leslie Progerin guilty of size discrimination ?? Mitch Leslie Why metaphase chromosomes wander . . . . ?? Mitch Leslie Three's not a crowd for actin nucleators ?? Mitch Leslie Making short work of p53 . . . . . . . . 491 Caitlin Sedwick Victor Ambros: The broad scope of microRNAs . . . . . . . . . . . . . . . 492 Helmut Krämer Route to destruction: Autophagosomes SNARE lysosomes . . . . . . . . . . . . 495 Fedik Rahimov and Louis M. Kunkel Cellular and molecular mechanisms underlying muscular dystrophy . . . . . 499 Petra Kleiblova and Indra A. Shaltiel and Jan Benada and Jan ev\vcík and So\vna Pechá\vcková and Petr Pohlreich and Emile E. Voest and Pavel Dundr and Jiri Bartek and Zdenek Kleibl and René H. Medema and Libor Macurek Gain-of-function mutations of PPM1D/Wip1 impair the p53-dependent G1 checkpoint 511 Mary Rose Bufalino and Brian DeVeale and Derek van der Kooy The asymmetric segregation of damaged proteins is stem cell--type dependent 523 Szabolcs Takáts and Péter Nagy and Ágnes Varga and Karolina Pircs and Manuéla Kárpáti and Kata Varga and Attila L. Kovács and Krisztina Heged\Hus and Gábor Juhász Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in \bionameDrosophila . . . . . 531 Chelsi J. Snow and Ashraf Dar and Anindya Dutta and Ralph H. Kehlenbach and Bryce M. Paschal Defective nuclear import of Tpr in Progeria reflects the Ran sensitivity of large cargo transport . . . . . . . . . 541 Elsa Kress and Françoise Schwager and René Holtackers and Jonas Seiler and François Prodon and Esther Zanin and Annika Eiteneuer and Mika Toya and Asako Sugimoto and Hemmo Meyer and Patrick Meraldi and Monica Gotta The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A 559 Gul Civelekoglu-Scholey and Bin He and Muyao Shen and Xiaohu Wan and Emanuele Roscioli and Brent Bowden and Daniela Cimini Dynamic bonds and polar ejection force distribution explain kinetochore oscillations in PtK1 cells . . . . . . . 577 Brian R. Graziano and Erin M. Jonasson and Jessica G. Pullen and Christopher J. Gould and Bruce L. Goode Ligand-induced activation of a formin--NPF pair leads to collaborative actin nucleation . . . . . . . . . . . . 595 Albert R. Davalos and Misako Kawahara and Gautam K. Malhotra and Nicholas Schaum and Jiahao Huang and Urvi Ved and Christian M. Beausejour and Jean-Philippe Coppe and Francis Rodier and Judith Campisi p53-dependent release of Alarmin HMGB1 is a central mediator of senescent phenotypes . . . . . . . . . . . . . . . 613 Daniel Chen and Emily S. Gibson and Matthew J. Kennedy A light-triggered protein secretion system . . . . . . . . . . . . . . . . . 631 Gregory Bix and Jian Fu and Eva M. Gonzalez and Laura Macro and Amy Barker and Shelly Campbell and Mary M. Zutter and Samuel A. Santoro and Jiyeun K. Kim and Magnus Höök and Charles C. Reed and Renato V. Iozzo Endorepellin causes endothelial cell disassembly of actin cytoskeleton and focal adhesions through $ \alpha $2$ \beta $1 integrin . . . . . . . . . . . 641 Molly Brunner and Angélique Millon-Frémillon and Genevieve Chevalier and Inaam A. Nakchbandi and Deane Mosher and Marc R. Block and Corinne Albig\`es-Rizo and Daniel Bouvard Osteoblast mineralization requires $ \beta $1 integrin/ICAP-$1$-dependent fibronectin deposition . . . . . . . . . 643
Ben Short Low pH puts proteasomes in storage . . . ?? Ben Short EB3 helps daughter cells spread their wings . . . . . . . . . . . . . . . . . ?? Ben Short Hif-1$ \alpha $ provides a Twist to neural crest migration . . . . . . . . . ?? Ben Short Stem cells get a cholesterol test . . . 647 Caitlin Sedwick Alpha Yap: Cadherins hold it all together . . . . . . . . . . . . . . . . 648 Tom Misteli Eliminating the impact of the Impact Factor . . . . . . . . . . . . . . . . . 651 Soizic Riche and Melissa Zouak and Françoise Argoul and Alain Arneodo and Jacques Pecreaux and Marie Delattre Evolutionary comparisons reveal a positional switch for spindle pole oscillations in \bionameCaenorhabditis embryos . . . . . . . . . . . . . . . . 653 Lee Zeev Peters and Rotem Hazan and Michal Breker and Maya Schuldiner and Shay Ben-Aroya Formation and dissociation of proteasome storage granules are regulated by cytosolic pH . . . . . . . . . . . . . . 663 Felix Rivera-Molina and Derek Toomre Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion . . . . . . . . 673 Cornelia Kröger and Fanny Loschke and Nicole Schwarz and Reinhard Windoffer and Rudolf E. Leube and Thomas M. Magin Keratins control intercellular adhesion involving PKC-$ \alpha $-mediated desmoplakin phosphorylation . . . . . . 681 Colleen R. Reczek and Matthias Szabolcs and Jeremy M. Stark and Thomas Ludwig and Richard Baer The interaction between CtIP and BRCA1 is not essential for resection-mediated DNA repair or tumor suppression . . . . 693 Jorge G. Ferreira and António J. Pereira and Anna Akhmanova and Helder Maiato Aurora B spatially regulates EB3 phosphorylation to coordinate daughter cell adhesion with cytokinesis . . . . . 709 Rubén Fernández-Busnadiego and Shoh Asano and Ana-Maria Oprisoreanu and Eri Sakata and Michael Doengi and Zdravko Kochovski and Magdalena Zürner and Valentin Stein and Susanne Schoch and Wolfgang Baumeister and Vladan Lu\vci\'c Cryo--electron tomography reveals a critical role of RIM1$ \alpha $ in synaptic vesicle tethering . . . . . . . 725 Tiffiney R. Hartman and Todd I. Strochlic and Yingbiao Ji and Daniel Zinshteyn and Alana M. O'Reilly Diet controls \bionameDrosophila follicle stem cell proliferation via Hedgehog sequestration and release . . . 741 Elias H. Barriga and Patrick H. Maxwell and Ariel E. Reyes and Roberto Mayor The hypoxia factor Hif-1$ \alpha $ controls neural crest chemotaxis and epithelial to mesenchymal transition . . 759 Fabien Vanden Abeele and Gabriel Bidaux and Dmitri Gordienko and Benjamin Beck and Yuri V. Panchin and Ancha V. Baranova and Dmitry V. Ivanov and Roman Skryma and Natalia Prevarskaya Functional implications of calcium permeability of the channel formed by pannexin 1 . . . . . . . . . . . . . . . 777
Mitch Leslie Hippo throws its weight behind migrating cells . . . . . . . . . . . . . . . . . ?? Mitch Leslie E-cadherin stops embryonic cells from crossing borders . . . . . . . . . . . . ?? Mitch Leslie Bone morphogenetic protein guts it out ?? Mitch Leslie Means to an end . . . . . . . . . . . . 781 Caitlin Sedwick Yukiko Yamashita: The centrosomes get there first . . . . . . . . . . . . . . 782 Kellie R. Machlus and Joseph E. Italiano The incredible journey: From megakaryocyte development to platelet formation . . . . . . . . . . . . . . . 785 Sara L. Poulsen and Rebecca K. Hansen and Sebastian A. Wagner and Loes van Cuijk and Gijsbert J. van Belle and Werner Streicher and Mats Wikström and Chunaram Choudhary and Adriaan B. Houtsmuller and Jurgen A. Marteijn and Simon Bekker-Jensen and Niels Mailand RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response . . . . . . . . . . 797 Jacob G. Kirkland and Rohinton T. Kamakaka Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins 809 Géraldine Laloux and Christine Jacobs-Wagner Spatiotemporal control of PopZ localization through cell cycle--coupled multimerization . . . . . . . . . . . . 827 Noel Lianga and Elizabeth C. Williams and Erin K. Kennedy and Carole Doré and Sophie Pilon and Stéphanie L. Girard and Jean-Sebastien Deneault and Adam D. Rudner A Wee1 checkpoint inhibits anaphase onset . . . . . . . . . . . . . . . . . 843 Yunhao Liu and Caitlin Collins and William B. Kiosses and Ann M. Murray and Monika Joshi and Tyson R. Shepherd and Ernesto J. Fuentes and Ellie Tzima A novel pathway spatiotemporally activates Rac1 and redox signaling in response to fluid shear stress . . . . . 863 Eliana P. Lucas and Ichha Khanal and Pedro Gaspar and Georgina C. Fletcher and Cedric Polesello and Nicolas Tapon and Barry J. Thompson The Hippo pathway polarizes the actin cytoskeleton during collective migration of \bionameDrosophila border cells . . . 875 Natalia A. Bulgakova and Ilya Grigoriev and Alpha S. Yap and Anna Akhmanova and Nicholas H. Brown Dynamic microtubules produce an asymmetric E--cadherin--Bazooka complex to maintain segment boundaries . . . . . 887 Elliott J. Hagedorn and Joshua W. Ziel and Meghan A. Morrissey and Lara M. Linden and Zheng Wang and Qiuyi Chi and Sam A. Johnson and David R. Sherwood The netrin receptor DCC focuses invadopodia-driven basement membrane transmigration in vivo . . . . . . . . . 903 Samantha A. Spangler and Sabine K. Schmitz and Josta T. Kevenaar and Esther de Graaff and Heidi de Wit and Jeroen Demmers and Ruud F. Toonen and Casper C. Hoogenraad Liprin-$ \alpha $2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission . . . . . . . . . . . . . . 915 Jooyeon Woo and Seok-Kyu Kwon and Jungyong Nam and Seungwon Choi and Hideto Takahashi and Dilja Krueger and Joohyun Park and Yeunkum Lee and Jin Young Bae and Dongmin Lee and Jaewon Ko and Hyun Kim and Myoung-Hwan Kim and Yong Chul Bae and Sunghoe Chang and Ann Marie Craig and Eunjoon Kim The adhesion protein IgSF9b is coupled to neuroligin 2 via S--SCAM to promote inhibitory synapse development . . . . . 929 Zheng Guo and Ian Driver and Benjamin Ohlstein Injury-induced BMP signaling negatively regulates \bionameDrosophila midgut homeostasis . . . . . . . . . . . . . . 945
Ben Short A potato virus makes its move . . . . . ?? Ben Short A mature view of the Golgi . . . . . . . ?? Ben Short Sequestration puts Drp1 on furlough . . ?? Ben Short Testing the limits of cell migration . . 965 Caitlin Sedwick John Briggs: a closer look at HIV and coated vesicles . . . . . . . . . . . . 966 Roberto Weigert and Natalie Porat-Shliom and Panomwat Amornphimoltham Imaging cell biology in live animals: Ready for prime time . . . . . . . . . . 969 Jens Tilsner and Olga Linnik and Marion Louveaux and Ian M. Roberts and Sean N. Chapman and Karl J. Oparka Replication and trafficking of a plant virus are coupled at the entrances of plasmodesmata . . . . . . . . . . . . . 981 Grzegorz Nalepa and Jill Barnholtz-Sloan and Rikki Enzor and Dilip Dey and Ying He and Jeff R. Gehlhausen and Amalia S. Lehmann and Su-Jung Park and Yanzhu Yang and Xianlin Yang and Shi Chen and Xiaowei Guan and Yanwen Chen and Jamie Renbarger and Feng-Chun Yang and Luis F. Parada and Wade Clapp The tumor suppressor CDKN3 controls mitosis . . . . . . . . . . . . . . . . 997 Linda Clijsters and Janneke Ogink and Rob Wolthuis The spindle checkpoint, APC/C$^{\rm Cdc20}$, and APC/C$^{\rm Cdh1}$ play distinct roles in connecting mitosis to S phase . . . . . . . . . . . . . . . . 1013 Riccardo Rizzo and Seetharaman Parashuraman and Peppino Mirabelli and Claudia Puri and John Lucocq and Alberto Luini The dynamics of engineered resident proteins in the mammalian Golgi complex relies on cisternal maturation . . . . . 1027 Stefan Strack and Theodore J. Wilson and J. Thomas Cribbs Cyclin-dependent kinases regulate splice-specific targeting of dynamin-related protein 1 to microtubules . . . . . . . . . . . . . . 1037 Jieqing Zhu and Jianghai Zhu and Timothy A. Springer Complete integrin headpiece opening in eight steps . . . . . . . . . . . . . . 1053 Katarina Wolf and Mariska te Lindert and Marina Krause and Stephanie Alexander and Joost te Riet and Amanda L. Willis and Robert M. Hoffman and Carl G. Figdor and Stephen J. Weiss and Peter Friedl Physical limits of cell migration: Control by ECM space and nuclear deformation and tuning by proteolysis and traction force . . . . . . . . . . . 1069 Oxana E. Nekrasova and Evangeline V. Amargo and William O. Smith and Jing Chen and Geri E. Kreitzer and Kathleen J. Green Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes 1085 Yeon-Tae Jeong and Mario Rossi and Lukas Cermak and Anita Saraf and Laurence Florens and Michael P. Washburn and Patrick Sung and Carl L. Schildkraut and Michele Pagano FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress . . . . . . . . . . . 1087
Mitch Leslie Catching augmin in the act . . . . . . . ?? Mitch Leslie $ \beta $-Catenin solidifies tight junctions in the skin . . . . . . . . . ?? Mitch Leslie SSX2IP helps the centrosome grow up . . ?? Mitch Leslie A lifeline for lipid rafts? . . . . . . 3 Caitlin Sedwick Didier Stainier: How function follows form . . . . . . . . . . . . . . . . . . 4 Inge Kepert and Michael A. Kiebler MoniTORing neuronal excitability at the synapse . . . . . . . . . . . . . . . . 7 Darren J. Baker and John M. Sedivy Probing the depths of cellular senescence . . . . . . . . . . . . . . . 11 Masahide Kikkawa Big steps toward understanding dynein 15 Tomoko Kamasaki and Eileen O'Toole and Shigeo Kita and Masako Osumi and Jiro Usukura and J. Richard McIntosh and Gohta Goshima Augmin-dependent microtubule nucleation at microtubule walls in the spindle . . 25 Alexandre Toulmay and William A. Prinz Direct imaging reveals stable, micrometer-scale lipid domains that segregate proteins in live cells . . . . 35 Samriddha Ray and Henry P. Foote and Terry Lechler $ \beta $-Catenin protects the epidermis from mechanical stresses . . . . . . . . 45 Natasha M. Sosanya and Peggy P. C. Huang and Luisa P. Cacheaux and Chun Jung Chen and Kathleen Nguyen and Nora I. Perrone-Bizzozero and Kimberly F. Raab-Graham Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1 . . . . . . . . . . 53 Archana Jha and Malini Ahuja and József Maléth and Claudia M. Moreno and Joseph P. Yuan and Min Seuk Kim and Shmuel Muallem The STIM1 CTID domain determines access of SARAF to SOAR to regulate Orai1 channel function . . . . . . . . . . . . 71 Felix Bärenz and Daigo Inoue and Hideki Yokoyama and Justus Tegha-Dunghu and Stephanie Freiss and Stefanie Draeger and Dmytro Mayilo and Ivana Cado and Sabine Merker and Maren Klinger and Burkhard Hoeckendorf and Sahra Pilz and Kerstin Hupfeld and Herbert Steinbeisser and Holger Lorenz and Thomas Ruppert and Joachim Wittbrodt and Oliver J. Gruss The centriolar satellite protein SSX2IP promotes centrosome maturation . . . . . 81 Guangzuo Luo and Jian Zhang and Francis C. Luca and Wei Guo Mitotic phosphorylation of Exo84 disrupts exocyst assembly and arrests cell growth . . . . . . . . . . . . . . 97 Mehul Shah and Oscar Y. Baterina and Vanessa Taupin and Marilyn G. Farquhar ARH directs megalin to the endocytic recycling compartment to regulate its proteolysis and gene expression . . . . 113 Andre Ivanov and Jeff Pawlikowski and Indrani Manoharan and John van Tuyn and David M. Nelson and Taranjit Singh Rai and Parisha P. Shah and Graeme Hewitt and Viktor I. Korolchuk and Joao F. Passos and Hong Wu and Shelley L. Berger and Peter D. Adams Lysosome-mediated processing of chromatin in senescence . . . . . . . . 129 Yuko Fukata and Ariane Dimitrov and Gaelle Boncompain and Ole Vielemeyer and Franck Perez and Masaki Fukata Local palmitoylation cycles define activity-regulated postsynaptic subdomains . . . . . . . . . . . . . . . 145 Ingo Thievessen and Peter M. Thompson and Sylvain Berlemont and Karen M. Plevock and Sergey V. Plotnikov and Alice Zemljic-Harpf and Robert S. Ross and Michael W. Davidson and Gaudenz Danuser and Sharon L. Campbell and Clare M. Waterman Vinculin--actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth . . . . . . . . . . . . 163 Kohei Yamamizu and Taichi Matsunaga and Hideki Uosaki and Hiroyuki Fukushima and Shiori Katayama and Mina Hiraoka-Kanie and Kohnosuke Mitani and Jun K. Yamashita Convergence of Notch and $ \beta $-catenin signaling induces arterial fate in vascular progenitors . . . . . . 179
Ben Short CDK1 makes the meiotic spindle wait . . ?? Ben Short Moving the Greatwall . . . . . . . . . . ?? Ben Short Epithelia restored by healing waves . . ?? Ben Short Syntaphilin puts the brakes on axonal mitochondria . . . . . . . . . . . . . . 183 Caitlin Sedwick Suzanne Eaton: The beautiful logic of development . . . . . . . . . . . . . . 184 Mohan K. Balasubramanian and Evelyn Yaqiong Tao Timing it right: Precise ON/OFF switches for Rho1 and Cdc42 GTPases in cytokinesis . . . . . . . . . . . . . . 187 Francis A. Barr Rab GTPases and membrane identity: Causal or inconsequential? . . . . . . . 191 Benjamin Albert and Julien Mathon and Ashutosh Shukla and Hicham Saad and Christophe Normand and Isabelle Léger-Silvestre and David Villa and Alain Kamgoue and Julien Mozziconacci and Hua Wong and Christophe Zimmer and Purnima Bhargava and Aurélien Bancaud and Olivier Gadal Systematic characterization of the conformation and dynamics of budding yeast chromosome XII . . . . . . . . . . 201 Yi-Nan Lin and Chien-Ting Wu and Yu-Chih Lin and Wen-Bin Hsu and Chieh-Ju C. Tang and Ching-Wen Chang and Tang K. Tang CEP120 interacts with CPAP and positively regulates centriole elongation . . . . . . . . . . . . . . . 211 Olga Davydenko and Richard M. Schultz and Michael A. Lampson Increased CDK1 activity determines the timing of kinetochore-microtubule attachments in meiosis I . . . . . . . . 221 Benjamin D. Atkins and Satoshi Yoshida and Koji Saito and Chi-Fang Wu and Daniel J. Lew and David Pellman Inhibition of Cdc42 during mitotic exit is required for cytokinesis . . . . . . 231 Yuichi Wakana and Julien Villeneuve and Josse van Galen and David Cruz-Garcia and Mitsuo Tagaya and Vivek Malhotra Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface . . . . . . . . . . . . 241 Sara Solinet and Kazi Mahmud and Shannon F. Stewman and Khaled Ben El Kadhi and Barbara Decelle and Lama Talje and Ao Ma and Benjamin H. Kwok and Sébastien Carreno The actin-binding ERM protein Moesin binds to and stabilizes microtubules at the cell cortex . . . . . . . . . . . . 251 Todd Schoborg and Ryan Rickels and Josh Barrios and Mariano Labrador Chromatin insulator bodies are nuclear structures that form in response to osmotic stress and cell death . . . . . 261 Peng Wang and Jacob A. Galan and Karine Normandin and Éric Bonneil and Gilles R. Hickson and Philippe P. Roux and Pierre Thibault and Vincent Archambault Cell cycle regulation of Greatwall kinase nuclear localization facilitates mitotic progression . . . . . . . . . . 277 Inai Park and Hae-ock Lee and Eunhee Choi and Yoo-Kyung Lee and Mi-Sun Kwon and Jaewon Min and Pil-Gu Park and Seonju Lee and Young-Yun Kong and Gyungyub Gong and Hyunsook Lee Loss of BubR1 acetylation causes defects in spindle assembly checkpoint signaling and promotes tumor formation . . . . . . 295 Masayuki Onishi and Nolan Ko and Ryuichi Nishihama and John R. Pringle Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis . . . . . . . . 311 Helene Knævelsrud and Kristiane Sòreng and Camilla Raiborg and Karin Håberg and Fredrik Rasmuson and Andreas Brech and Knut Liestòl and Tor Erik Rusten and Harald Stenmark and Thomas P. Neufeld and Sven R. Carlsson and Anne Simonsen Membrane remodeling by the PX--BAR protein SNX18 promotes autophagosome formation . . . . . . . . . . . . . . . 331 Yanmin Chen and Zu-Hang Sheng Kinesin-1-syntaphilin coupling mediates activity-dependent regulation of axonal mitochondrial transport . . . . . . . . 351 Marco Antunes and Telmo Pereira and João V. Cordeiro and Luis Almeida and Antonio Jacinto Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding . . . . . . . . . . . . . 365 Zu-Lin Chen and Yao Yao and Erin H. Norris and Anna Kruyer and Odella Jno-Charles and Akbarshakh Akhmerov and Sidney Strickland Ablation of astrocytic laminin impairs vascular smooth muscle cell function and leads to hemorrhagic stroke . . . . . . 381
Ben Short When two kinases are better than one . . ?? Ben Short Lis1 puts mRNAs in their place . . . . . 398 Ben Short Spastin coordinates the recycling program . . . . . . . . . . . . . . . . ?? Ben Short The CCM complex puts a cap on integrin activity . . . . . . . . . . . . . . . . 399 Caitlin Sedwick Bob Goldstein: Cell biology by way of development . . . . . . . . . . . . . . 400 Xavier Deschênes-Simard and Yusuke Mizukami and Nabeel Bardeesy Macrophages in pancreatic cancer: Starting things off on the wrong track 403 Vladan Lu\vci\'c and Alexander Rigort and Wolfgang Baumeister Cryo-electron tomography: The challenge of doing structural biology in situ . . 407 Sophie Vidal-Eychenié and Chantal Décaillet and Jihane Basbous and Angelos Constantinou DNA structure-specific priming of ATR activation by DNA--PKcs . . . . . . . . 421 José-Eduardo Gomes and Nicolas Tavernier and Bénédicte Richaudeau and Etienne Formstecher and Thomas Boulin and Paul E. Mains and Julien Dumont and Lionel Pintard Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation . . . . . . . . 431 Montserrat Bosch Grau and Gloria Gonzalez Curto and Cecilia Rocha and Maria M. Magiera and Patricia Marques Sousa and Tiziana Giordano and Nathalie Spassky and Carsten Janke Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia . . . . . . 441 Konstantinos Lefkimmiatis and Daniela Leronni and Aldebaran M. Hofer The inner and outer compartments of mitochondria are sites of distinct cAMP/PKA signaling dynamics . . . . . . 453 Fiona E. Hood and Samantha J. Williams and Selena G. Burgess and Mark W. Richards and Daniel Roth and Anne Straube and Mark Pfuhl and Richard Bayliss and Stephen J. Royle Coordination of adjacent domains mediates TACC3--ch--TOG--clathrin assembly and mitotic spindle binding . . 463 Carly I. Dix and Harish Chandra Soundararajan and Nikola S. Dzhindzhev and Farida Begum and Beat Suter and Hiroyuki Ohkura and Elaine Stephens and Simon L. Bullock Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs . . . . . . . . . . . . . . . . . 479 Meng-meng Fu and Erika L. F. Holzbaur JIP1 regulates the directionality of APP axonal transport by coordinating kinesin and dynein motors . . . . . . . . . . . 495 David Shen and Hua Yuan and Alex Hutagalung and Avani Verma and Daniel Kümmel and Xudong Wu and Karin Reinisch and James A. McNew and Peter Novick The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p . . . . . . 509 Rachel Allison and Jennifer H. Lumb and Coralie Fassier and James W. Connell and Daniel Ten Martin and Matthew N. J. Seaman and Jamilé Hazan and Evan Reid An ESCRT--spastin interaction promotes fission of recycling tubules from the endosome . . . . . . . . . . . . . . . . 527 Eva Faurobert and Claire Rome and Justyna Lisowska and Sandra Manet-Dupé and Gwénola Boulday and Marilyne Malbouyres and Martial Balland and Anne-Pascale Bouin and Michelle Kéramidas and Daniel Bouvard and Jean-Luc Coll and Florence Ruggiero and Elisabeth Tournier-Lasserve and Corinne Albiges-Rizo CCM1--ICAP-$1$ complex controls $ \beta $1 integrin-dependent endothelial contractility and fibronectin remodeling 545 Geou-Yarh Liou and Heike Döppler and Brian Necela and Murli Krishna and Howard C. Crawford and Massimo Raimondo and Peter Storz Macrophage-secreted cytokines drive pancreatic acinar-to-ductal metaplasia through NF-$ \kappa $B and MMPs . . . . 563 Sébastien Britton and Julia Coates and Stephen P. Jackson A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair . . . . . . . 579 Gul Civelekoglu-Scholey and Bin He and Muyao Shen and Xiaohu Wan and Emanuele Roscioli and Brent Bowden and Daniela Cimini Dynamic bonds and polar ejection force distribution explain kinetochore oscillations in PtK1 cells . . . . . . . 597
Ben Short Epiblast cells CLASP onto the basement membrane . . . . . . . . . . . . . . . . ?? Ben Short Bruchpilot readies synaptic vesicles for release . . . . . . . . . . . . . . . . ?? Ben Short Phosphorylation helps Atg18 get the vacuole in shape . . . . . . . . . . . . ?? Ben Short Aurora B helps the central spindle measure up . . . . . . . . . . . . . . . 601 Caitlin Sedwick Jody Rosenblatt: To extrude apically or basally, that is the question . . . . . 602 Ricardo Nunes Bastos and Sapan R. Gandhi and Ryan D. Baron and Ulrike Gruneberg and Erich A. Nigg and Francis A. Barr Aurora B suppresses microtubule dynamics and limits central spindle size by locally activating KIF4A . . . . . . . . 605 Ryota Uehara and Yuki Tsukada and Tomoko Kamasaki and Ina Poser and Kinya Yoda and Daniel W. Gerlich and Gohta Goshima Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase . . . . 623 Yukiko Nakaya and Erike W. Sukowati and Guojun Sheng Epiblast integrity requires CLASP and Dystroglycan-mediated microtubule anchoring to the basal cortex . . . . . 637 Adi D. Dubash and Jennifer L. Koetsier and Evangeline V. Amargo and Nicole A. Najor and Robert M. Harmon and Kathleen J. Green The GEF Bcr activates RhoA/MAL signaling to promote keratinocyte differentiation via desmoglein-1 . . . . . . . . . . . . 653 Tanja Matkovic and Matthias Siebert and Elena Knoche and Harald Depner and Sara Mertel and David Owald and Manuela Schmidt and Ulrich Thomas and Albert Sickmann and Dirk Kamin and Stefan W. Hell and Jörg Bürger and Christina Hollmann and Thorsten Mielke and Carolin Wichmann and Stephan J. Sigrist The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles . . . . . . . . . . . 667 Naoki Tamura and Masahide Oku and Moemi Ito and Nobuo N. Noda and Fuyuhiko Inagaki and Yasuyoshi Sakai Atg18 phosphoregulation controls organellar dynamics by modulating its phosphoinositide-binding activity . . . 685 Nuno Saraiva and David L. Prole and Guia Carrara and Benjamin F. Johnson and Colin W. Taylor and Maddy Parsons and Geoffrey L. Smith hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca$^{2+}$ entry and calpain 2 . . . . . . . . . . . . . . . 699 Beatriz G. Galvez and Maurilio Sampaolesi and Silvia Brunelli and Diego Covarello and Manuela Gavina and Barbara Rossi and Gabriela Constantin and Yvan Torrente and Giulio Cossu Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability . . . . . . . . . . . 715 Grzegorz Nalepa and Jill Barnholtz-Sloan and Rikki Enzor and Dilip Dey and Ying He and Jeff R. Gehlhausen and Amalia S. Lehmann and Su-Jung Park and Yanzhu Yang and Xianlin Yang and Shi Chen and Xiaowei Guan and Yanwen Chen and Jamie Renbarger and Feng-Chun Yang and Luis F. Parada and Wade Clapp The tumor suppressor CDKN3 controls mitosis . . . . . . . . . . . . . . . . 717
Mitch Leslie Injured neurons pump up the volume . . . ?? Mitch Leslie Drebrin shows self-restraint . . . . . . ?? Mitch Leslie Crawling cells feel the squeeze . . . . 721 Caitlin Sedwick Helder Maiato: Hot (+)TIPs on mitosis 722 Jonathan A. Cooper Mechanisms of cell migration in the nervous system . . . . . . . . . . . . . 725 Dileep Varma and Xiaohu Wan and Dhanya Cheerambathur and Reto Gassmann and Aussie Suzuki and Josh Lawrimore and Arshad Desai and E. D. Salmon Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores . . . . 735 Sarah Huntwork-Rodriguez and Bei Wang and Trent Watkins and Arundhati Sengupta Ghosh and Christine D. Pozniak and Daisy Bustos and Kim Newton and Donald S. Kirkpatrick and Joseph W. Lewcock JNK-mediated phosphorylation of DLK suppresses its ubiquitination to promote neuronal apoptosis . . . . . . . . . . . 747 Claudio Vernieri and Elena Chiroli and Valentina Francia and Fridolin Gross and Andrea Ciliberto Adaptation to the spindle checkpoint is regulated by the interplay between Cdc28/Clbs and PP2A$^{\rm Cdc55}$ . . . 765 Jason Yi and Xufeng Wu and Andrew H. Chung and James K. Chen and Tarun M. Kapoor and John A. Hammer Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage . . . . . . . . 779 Daniel C. Worth and Catherine N. Daly and Sara Geraldo and Fazal Oozeer and Phillip R. Gordon-Weeks Drebrin contains a cryptic F-actin--bundling activity regulated by Cdk5 phosphorylation . . . . . . . . . . 793 Wei-Chien Hung and Shih-Hsun Chen and Colin D. Paul and Kimberly M. Stroka and Ying-Chun Lo and Joy T. Yang and Konstantinos Konstantopoulos Distinct signaling mechanisms regulate migration in unconfined versus confined spaces . . . . . . . . . . . . . . . . . 807 Nadine Bakkar and Jingxin Wang and Katherine J. Ladner and Huating Wang and Jason M. Dahlman and Micheal Carathers and Swarnali Acharyya and Michael A. Rudnicki and Andrew D. Hollenbach and Denis C. Guttridge IKK\slash NF-$ \kappa $B regulates skeletal myogenesis via a signaling switch to inhibit differentiation and promote mitochondrial biogenesis . . . . 825
Mitch Leslie Drs2 flips the phospholipid switch . . . ?? Mitch Leslie Rap1 helps endothelial cells stay close ?? Mitch Leslie Silicon nanocrystals shine . . . . . . . ?? Mitch Leslie Creating cellular inequality . . . . . . 829 Caitlin Sedwick Frances Brodsky: So you think you know all about clathrin? . . . . . . . . . . 830 Christine Lawson and David D. Schlaepfer pHocal adhesion kinase regulation is on a FERM foundation . . . . . . . . . . . 833 Tommy L. Lewis and Julien Courchet and Franck Polleux Cellular and molecular mechanisms underlying axon formation, growth, and branching . . . . . . . . . . . . . . . 837 Chang-Hoon Choi and Bradley A. Webb and Michael S. Chimenti and Matthew P. Jacobson and Diane L. Barber pH sensing by FAK--His58 regulates focal adhesion remodeling . . . . . . . . . . 849 Kazutaka Araki and Shun-ichiro Iemura and Yukiko Kamiya and David Ron and Koichi Kato and Tohru Natsume and Kazuhiro Nagata Ero1-$ \alpha $ and PDIs constitute a hierarchical electron transfer network of endoplasmic reticulum oxidoreductases 861 Peng Xu and Ryan D. Baldridge and Richard J. Chi and Christopher G. Burd and Todd R. Graham Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport . . . . 875 Michaela T. Niessen and Jeanie Scott and Julia G. Zielinski and Susanne Vorhagen and Panagiota A. Sotiropoulou and Cédric Blanpain and Michael Leitges and Carien M. Niessen aPKC$ \lambda $ controls epidermal homeostasis and stem cell fate through regulation of division orientation . . . 887 Koji Ando and Shigetomo Fukuhara and Takahiro Moriya and Yutaro Obara and Norimichi Nakahata and Naoki Mochizuki Rap1 potentiates endothelial cell junctions by spatially controlling myosin II activity and actin organization . . . . . . . . . . . . . . 901 Guillaume Jacquemet and David M. Green and Rebecca E. Bridgewater and Alexander von Kriegsheim and Martin J. Humphries and Jim C. Norman and Patrick T. Caswell RCP-driven $ \alpha $5$ \beta $1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1--IQGAP1 complex . . . . . . . . . . . . . . . . 917 Qingwei Zhu and Yong Hwan Kim and Douglas Wang and S. Paul Oh and Kunxin Luo SnoN facilitates ALK1-Smad1/5 signaling during embryonic angiogenesis . . . . . 937 Daniel H. Madsen and Daniel Leonard and Andrius Masedunskas and Amanda Moyer and Henrik Jessen Jürgensen and Diane E. Peters and Panomwat Amornphimoltham and Arul Selvaraj and Susan S. Yamada and David A. Brenner and Sven Burgdorf and Lars H. Engelholm and Niels Behrendt and Kenn Holmbeck and Roberto Weigert and Thomas H. Bugge M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway . . . . . . . 951 Hirohito Nishimura and Ken Ritchie and Rinshi S. Kasai and Miki Goto and Nobuhiro Morone and Hiroyuki Sugimura and Koichiro Tanaka and Ichiro Sase and Akihiko Yoshimura and Yoshitaro Nakano and Takahiro K. Fujiwara and Akihiro Kusumi Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging . . . 967
Ben Short Determining the replication factory settings . . . . . . . . . . . . . . . . ?? Ben Short KASH5 helps meiotic chromosomes LINC up ?? Ben Short RNA granules act as egg timers . . . . . ?? Ben Short PLP helps the mother centrosome stay mum 987 Caitlin Sedwick Michael Sixt: Love the way they move . . 988 Toshiaki Shigeoka and Bo Lu and Christine E. Holt RNA-based mechanisms underlying axon guidance . . . . . . . . . . . . . . . . 991 Nazan Saner and Jens Karschau and Toyoaki Natsume and Marek Gierli\'nski and Renata Retkute and Michelle Hawkins and Conrad A. Nieduszynski and J. Julian Blow and Alessandro P. S. de Moura and Tomoyuki U. Tanaka Stochastic association of neighboring replicons creates replication factories in budding yeast . . . . . . . . . . . . 1001 Dorothy A. Lerit and Nasser M. Rusan PLP inhibits the activity of interphase centrosomes to ensure their proper segregation in stem cells . . . . . . . 1013 Henning F. Horn and Dae In Kim and Graham D. Wright and Esther Sook Miin Wong and Colin L. Stewart and Brian Burke and Kyle J. Roux A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton 1023 Tomoya Kotani and Kyota Yasuda and Ryoma Ota and Masakane Yamashita Cyclin B1 mRNA translation is temporally controlled through formation and disassembly of RNA granules . . . . . . 1041 Weiwei Luo and Cheng-han Yu and Zi Zhao Lieu and Jun Allard and Alex Mogilner and Michael P. Sheetz and Alexander D. Bershadsky Analysis of the local organization and dynamics of cellular actin networks . . 1057 Diego E. Alvarez and Hervé Agaisse The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility . . . . . . . . . . . . . . . . 1075 Sarah E. Smith and Boris Rubinstein and Inês Mendes Pinto and Brian D. Slaughter and Jay R. Unruh and Rong Li Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42 . . . . . . . . . . . . . . . . 1091 Constance S. Petit and Agnes Roczniak-Ferguson and Shawn M. Ferguson Recruitment of folliculin to lysosomes supports the amino acid-dependent activation of Rag GTPases . . . . . . . 1107 Qiaoqiao Xu and Shanshan Huang and Mingke Song and Chuan-En Wang and Sen Yan and Xudong Liu and Marta A. Gaertig and Shan Ping Yu and He Li and Shihua Li and Xiao-Jiang Li Synaptic mutant huntingtin inhibits synapsin-1 phosphorylation and causes neurological symptoms . . . . . . . . . 1123
Ben Short Histone H1 gets Pin'd onto chromatin . . ?? Ben Short MAD2L2 helps mitotic cells take it slow ?? Ben Short Ubiquitin isolates bacterial invaders ?? Ben Short Formin' the cytokinetic ring . . . . . . 3 Caitlin Sedwick Yohanns Bella\"\iche: Mastering multiscale morphology . . . . . . . . . 4 Yukiko M. Yamashita Nonrandom template segregation: a way to break the symmetry of stem cells . . . . 7 Poh Hui Chia and Pengpeng Li and Kang Shen Cellular and molecular mechanisms underlying presynapse formation . . . . 11 Joshua D. Ochocki and M. Celeste Simon Nutrient-sensing pathways and metabolic regulation in stem cells . . . . . . . . 23 Haifeng Wang and Ingrid Brust-Mascher and Gul Civelekoglu-Scholey and Jonathan M. Scholey Patronin mediates a switch from kinesin-$ 13$-dependent poleward flux to anaphase B spindle elongation . . . . . 35 Daniel Lafkas and Veronica Rodilla and Mathilde Huyghe and Larissa Mourao and Hippokratis Kiaris and Silvia Fre Notch3 marks clonogenic mammary luminal progenitor cells in vivo . . . . . . . . 47 Nikhil Raghuram and Hilmar Strickfaden and Darin McDonald and Kylie Williams and He Fang and Craig Mizzen and Jeffrey J. Hayes and John Th'ng and Michael J. Hendzel Pin1 promotes histone H1 dephosphorylation and stabilizes its binding to chromatin . . . . . . . . . . 57 Christian Elabd and Wendy Cousin and Robert Y. Chen and Marc S. Chooljian and Joey T. Pham and Irina M. Conboy and Michael J. Conboy DNA methyltransferase-$3$-dependent nonrandom template segregation in differentiating embryonic stem cells . . 73 Tamar Listovsky and Julian E. Sale Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset . . . . . . . . . . . . . 87 Valerie C. Coffman and Jennifer A. Sees and David R. Kovar and Jian-Qiu Wu The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis . . . . . . . . . . . . . . 101 Naonobu Fujita and Eiji Morita and Takashi Itoh and Atsushi Tanaka and Megumi Nakaoka and Yuki Osada and Tetsuo Umemoto and Tatsuya Saitoh and Hitoshi Nakatogawa and Shouhei Kobayashi and Tokuko Haraguchi and Jun-Lin Guan and Kazuhiro Iwai and Fuminori Tokunaga and Kazunobu Saito and Koutaro Ishibashi and Shizuo Akira and Mitsunori Fukuda and Takeshi Noda and Tamotsu Yoshimori Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin . . . . . . . . . 115 David K. Breslow and Elena F. Koslover and Federica Seydel and Andrew J. Spakowitz and Maxence V. Nachury An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier . . . . . . . . . . . 129 Ka-wing Fong and Yujing Li and Wenqi Wang and Wenbin Ma and Kunpeng Li and Robert Z. Qi and Dan Liu and Zhou Songyang and Junjie Chen Whole-genome screening identifies proteins localized to distinct nuclear bodies . . . . . . . . . . . . . . . . . 149 Cherilyn M. Sirois and Tengchuan Jin and Allison L. Miller and Damien Bertheloot and Hirotaka Nakamura and Gabor L. Horvath and Abubakar Mian and Jiansheng Jiang and Jacob Schrum and Lukas Bossaller and Karin Pelka and Natalio Garbi and Yambasu Brewah and Jane Tian and Chew-Shun Chang and Partha Chowdhury and Gary Sims and Roland Kolbeck and Anthony Coyle and Alison Humbles and T. Sam Xiao and Eicke Latz RAGE is a nucleic acid receptor that promotes inflammatory responses to DNA ?? Helena Soares and Ricardo Henriques and Martin Sachse and Leandro Ventimiglia and Miguel A. Alonso and Christophe Zimmer and Maria-Isabel Thoulouze and Andres Alcover Regulated vesicle fusion generates signaling nanoterritories that control T-cell activation at the immunological synapse . . . . . . . . . . . . . . . . ?? Xuan Zhou and Maria Carolina Florian and Paritha Arumugam and Xiaoyi Chen and Jose A. Cancelas and Richard Lang and Punam Malik and Hartmut Geiger and Yi Zheng RhoA GTPase controls cytokinesis and programmed necrosis of hematopoietic progenitors . . . . . . . . . . . . . . ?? Dong Wang and Damien O'Halloran and Miriam B. Goodman GCY-8, PDE-2, and NCS-1 are critical elements of the cGMP-dependent thermotransduction cascade in the AFD neurons responsible for \bionameC. elegans thermotaxis . . . . . . . . . . ??
Mitch Leslie Putting Cyclin E in the trash . . . . . ?? Mitch Leslie Polysaccharide's central role in cell division . . . . . . . . . . . . . . . . ?? Mitch Leslie Dynamin 2 cuts the cord for newborn lysosome . . . . . . . . . . . . . . . . ?? Mitch Leslie Ensuring that yeast cells get their inheritance . . . . . . . . . . . . . . 167 Caitlin Sedwick Daniel Colón--Ramos: Observing and making connections . . . . . . . . . . . . . . 168 Dong Wang and P. Robin Hiesinger The vesicular ATPase: a missing link between acidification and exocytosis . . 171 Nathalia Vitureira and Yukiko Goda The interplay between Hebbian and homeostatic synaptic plasticity . . . . 175 Tadashi Makio and Diego L. Lapetina and Richard W. Wozniak Inheritance of yeast nuclear pore complexes requires the Nsp1p subcomplex 187 Jennifer E. Dulle and Rachel E. Bouttenot and Lisa A. Underwood and Heather L. True Soluble oligomers are sufficient for transmission of a yeast prion but do not confer phenotype . . . . . . . . . . . . 197 Sarah Oddoux and Kristien J. Zaal and Victoria Tate and Aster Kenea and Shuktika A. Nandkeolyar and Ericka Reid and Wenhua Liu and Evelyn Ralston Microtubules that form the stationary lattice of muscle fibers are dynamic and nucleated at Golgi elements . . . . . . 205 Paolo Colombi and Brant M. Webster and Florian Fröhlich and C. Patrick Lusk The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1 . . . . . . . . 215 Albert Lu and Suzanne R. Pfeffer Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression . . . . . . . . . . . 233 Francisco Lázaro-Diéguez and David Cohen and Dawn Fernandez and Louis Hodgson and Sven C. D. van IJzendoorn and Anne Müsch Par1b links lumen polarity with LGN--NuMA positioning for distinct epithelial cell division phenotypes . . 251 Javier Muñoz and Juan Carlos G. Cortés and Matthias Sipiczki and Mariona Ramos and José Angel Clemente-Ramos and M. Belén Moreno and Ivone M. Martins and Pilar Pérez and Juan Carlos Ribas Extracellular cell wall $ \beta $ (1,3)glucan is required to couple septation to actomyosin ring contraction 265 Sandrine Poëa-Guyon and Mohamed Raafet Ammar and Marie Erard and Muriel Amar and Alexandre W. Moreau and Philippe Fossier and Vincent Gleize and Nicolas Vitale and Nicolas Morel The V--ATPase membrane domain is a sensor of granular pH that controls the exocytotic machinery . . . . . . . . . . 283 Yasuhiro Araki and Wei-Chi Ku and Manami Akioka and Alexander I. May and Yu Hayashi and Fumio Arisaka and Yasushi Ishihama and Yoshinori Ohsumi Atg38 is required for autophagy-specific phosphatidylinositol $3$-kinase complex integrity . . . . . . . . . . . . . . . 299 Ryan J. Schulze and Shaun G. Weller and Barbara Schroeder and Eugene W. Krueger and Susan Chi and Carol A. Casey and Mark A. McNiven Lipid droplet breakdown requires Dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes . . . . . . . . . 315 Bhaskar Ponugoti and Fanxing Xu and Chenying Zhang and Chen Tian and Sandra Pacios and Dana T. Graves FOXO1 promotes wound healing through the up-regulation of TGF-$ \beta $1 and prevention of oxidative stress . . . . . 327 Catherine E. Winbanks and Justin L. Chen and Hongwei Qian and Yingying Liu and Bianca C. Bernardo and Claudia Beyer and Kevin I. Watt and Rachel E. Thomson and Timothy Connor and Bradley J. Turner and Julie R. McMullen and Lars Larsson and Sean L. McGee and Craig A. Harrison and Paul Gregorevic The bone morphogenetic protein axis is a positive regulator of skeletal muscle mass . . . . . . . . . . . . . . . . . . 345 Benedetta Cerruti and Alberto Puliafito and Annette M. Shewan and Wei Yu and Alexander N. Combes and Melissa H. Little and Federica Chianale and Luca Primo and Guido Serini and Keith E. Mostov and Antonio Celani and Andrea Gamba Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures . . . . 359 Vincent Mirouse and Lance L. Swick and Nevzat Kazgan and Daniel St Johnston and Jay E. Brenman LKB1 and AMPK maintain epithelial cell polarity under energetic stress . . . . 373
Ben Short Yeast centromeres coordinate their movements . . . . . . . . . . . . . . . ?? Ben Short FRETting over kinesin activity . . . . . ?? Ben Short Switching on synaptic PP1 . . . . . . . ?? Ben Short Sortilins get a new delivery route . . . 377 Caitlin Sedwick Chris Bakal: Look and learn . . . . . . 378 Yael Eshed-Eisenbach and Elior Peles Axonal spectrins: All-purpose fences . . 381 Martijn P. J. Dekkers and Vassiliki Nikoletopoulou and Yves-Alain Barde Death of developing neurons: New insights and implications for connectivity . . . . . . . . . . . . . . 385 Megan M. Corty and Marc R. Freeman Architects in neural circuit design: Glia control neuron numbers and connectivity . . . . . . . . . . . . . . 395 Andrew D. Stephens and Chloe E. Snider and Julian Haase and Rachel A. Haggerty and Paula A. Vasquez and M. Gregory Forest and Kerry Bloom Individual pericentromeres display coordinated motion and stretching in the yeast spindle . . . . . . . . . . . . . 407 Kazuhiro Tateishi and Yuji Yamazaki and Tomoki Nishida and Shin Watanabe and Koshi Kunimoto and Hiroaki Ishikawa and Sachiko Tsukita Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains . . . . . . . . . 417 Michael D. Huber and Paul W. Vesely and Kaustuv Datta and Larry Gerace Erlins restrict SREBP activation in the ER and regulate cellular cholesterol homeostasis . . . . . . . . . . . . . . 427 Chuansheng Zhang and Keiichiro Susuki and Daniel R. Zollinger and Jeffrey L. Dupree and Matthew N. Rasband Membrane domain organization of myelinated axons requires $ \beta $II spectrin . . . . . . . . . . . . . . . . 437 Cedric Espenel and Bipul R. Acharya and Geri Kreitzer A biosensor of local kinesin activity reveals roles of PKC and EB1 in KIF17 activation . . . . . . . . . . . . . . . 445 Nima Mosammaparast and Haeyoung Kim and Benoit Laurent and Yu Zhao and Hui Jun Lim and Mona C. Majid and Sebastian Dango and Yuying Luo and Kristina Hempel and Mathew E. Sowa and Steven P. Gygi and Hanno Steen and J. Wade Harper and Bruce Yankner and Yang Shi The histone demethylase LSD1/KDM1A promotes the DNA damage response . . . . 457 Andrew B. Lane and Juan F. Giménez-Abián and Duncan J. Clarke A novel chromatin tether domain controls topoisomerase II$ \alpha $ dynamics and mitotic chromosome formation . . . . . . 471 Nour El Amine and Amel Kechad and Silvana Jananji and Gilles R. X. Hickson Opposing actions of septins and Sticky on Anillin promote the transition from contractile to midbody ring . . . . . . 487 Rebecca A. Green and Jonathan R. Mayers and Shaohe Wang and Lindsay Lewellyn and Arshad Desai and Anjon Audhya and Karen Oegema The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules . . . . . . . . . . 505 Hailong Hou and Lu Sun and Benjamin A. Siddoway and Ronald S. Petralia and Hongtian Yang and Hua Gu and Angus C. Nairn and Houhui Xia Synaptic NMDA receptor stimulation activates PP1 by inhibiting its phosphorylation by Cdk5 . . . . . . . . 521 Joseph S. Briguglio and Santosh Kumar and Aaron P. Turkewitz Lysosomal sorting receptors are essential for secretory granule biogenesis in \bionameTetrahymena . . . 537
Ben Short Microtubules support nuclear nonproliferation arrangement . . . . . . ?? Ben Short AKT shuts down the TOR network . . . . . ?? Ben Short A call for oxygen in the ER . . . . . . ?? Ben Short Lamellipodin branches out . . . . . . . 553 Caitlin Sedwick Marilyn Farquhar: From the beginning . . 554 Ira Mellman and Scott D. Emr A Nobel Prize for membrane traffic: Vesicles find their journey's end . . . 559 Charles Betz and Michael N. Hall Where is mTOR and what is it doing there? . . . . . . . . . . . . . . . . . 563 Zhao Zhang and Jing Feng and Chenyu Pan and Xiangdong Lv and Wenqing Wu and Zhaocai Zhou and Feng Liu and Lei Zhang and Yun Zhao Atrophin--Rpd3 complex represses Hedgehog signaling by acting as a corepressor of Ci$^R$ . . . . . . . . . 575 Damien Laporte and Fabien Courtout and Bénédicte Salin and Johanna Ceschin and Isabelle Sagot An array of nuclear microtubules reorganizes the budding yeast nucleus during quiescence . . . . . . . . . . . 585 Lenka Hálová and Wei Du and Sara Kirkham and Duncan L. Smith and Janni Petersen Phosphorylation of the TOR ATP binding domain by AGC kinase constitutes a novel mode of TOR inhibition . . . . . . . . . 595 Tomoki Yano and Takeshi Matsui and Atsushi Tamura and Masami Uji and Sachiko Tsukita The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK . . . . . . . . 605 Marianne Koritzinsky and Fiana Levitin and Twan van den Beucken and Ryan A. Rumantir and Nicholas J. Harding and Kenneth C. Chu and Paul C. Boutros and Ineke Braakman and Bradly G. Wouters Two phases of disulfide bond formation have differing requirements for oxygen 615 Mehmet Sen and Koichi Yuki and Timothy A. Springer An internal ligand-bound, metastable state of a leukocyte integrin, $ \alpha_X $ $ \beta_2 $ . . . . . . . . . 629 Ashraf Yusuf Rangrez and Alexander Bernt and Reza Poyanmehr and Violetta Harazin and Inka Boomgaarden and Christian Kuhn and Astrid Rohrbeck and Derk Frank and Norbert Frey Dysbindin is a potent inducer of RhoA--SRF-mediated cardiomyocyte hypertrophy . . . . . . . . . . . . . . 643 Douglas S. Campbell and Hitoshi Okamoto Local caspase activation interacts with Slit--Robo signaling to restrict axonal arborization . . . . . . . . . . . . . . 657 Ah-Lai Law and Anne Vehlow and Maria Kotini and Lauren Dodgson and Daniel Soong and Eric Theveneau and Cristian Bodo and Eleanor Taylor and Christel Navarro and Upamali Perera and Magdalene Michael and Graham A. Dunn and Daimark Bennett and Roberto Mayor and Matthias Krause Lamellipodin and the Scar/WAVE complex cooperate to promote cell migration in vivo . . . . . . . . . . . . . . . . . . 673 Kai Safferling and Thomas Sütterlin and Kathi Westphal and Claudia Ernst and Kai Breuhahn and Merlin James and Dirk Jäger and Niels Halama and Niels Grabe Wound healing revised: a novel reepithelialization mechanism revealed by in vitro and in silico models . . . . 691
Mitch Leslie Shelterin protein keeps telomeres on edge . . . . . . . . . . . . . . . . . . ?? Mitch Leslie Actin-capping protein lets epithelial cells take the pressure . . . . . . . . ?? Mitch Leslie Sip1 liberates neural crest cells . . . ?? Mitch Leslie Silent RNAs express themselves . . . . . 713 Caitlin Sedwick Trina Schroer: What's cooking on dynactin . . . . . . . . . . . . . . . . 714 Camilla Godlee and Marko Kaksonen From uncertain beginnings: Initiation mechanisms of clathrin-mediated endocytosis . . . . . . . . . . . . . . 717 Helder C. Ferreira and Benjamin D. Towbin and Thibaud Jegou and Susan M. Gasser The shelterin protein POT-1 anchors \bionameCaenorhabditis elegans telomeres through SUN-1 at the nuclear periphery 727 Kyota Yasuda and Huaye Zhang and David Loiselle and Timothy Haystead and Ian G. Macara and Stavroula Mili The RNA-binding protein Fus directs translation of localized mRNAs in APC--RNP granules . . . . . . . . . . . 737 Joo-Hee Sir and Monika Pütz and Owen Daly and Ciaran G. Morrison and Mark Dunning and John V. Kilmartin and Fanni Gergely Loss of centrioles causes chromosomal instability in vertebrate somatic cells 747 Susana Romao and Nathalie Gasser and Andrea C. Becker and Bruno Guhl and Milica Bajagic and Danusia Vanoaica and Urs Ziegler and Joachim Roesler and Jörn Dengjel and Janine Reichenbach and Christian Münz Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing . . 757 Qian Bian and Nimish Khanna and Jurgis Alvikas and Andrew S. Belmont $ \beta $-Globin cis-elements determine differential nuclear targeting through epigenetic modifications . . . . . . . . 767 Anna Franz and Hélio Roque and Saroj Saurya and Jeroen Dobbelaere and Jordan W. Raff CP110 exhibits novel regulatory activities during centriole assembly in \bionameDrosophila . . . . . . . . . . . 785 Songyu Wang and Fabian B. Romano and Christine M. Field and Tim J. Mitchison and Tom A. Rapoport Multiple mechanisms determine ER network morphology during the cell cycle in \bionameXenopus egg extracts . . . . . . 801 Vivian W. Tang and William M. Brieher FSGS3\slash CD2AP is a barbed-end capping protein that stabilizes actin and strengthens adherens junctions . . . 815 Crystal D. Rogers and Ankur Saxena and Marianne E. Bronner Sip1 mediates an E--cadherin--to--N--cadherin switch during cranial neural crest EMT . . . . 835 Maike Kittelmann and Jan Hegermann and Alexandr Goncharov and Hidenori Taru and Mark H. Ellisman and Janet E. Richmond and Yishi Jin and Stefan Eimer Liprin-$ \alpha $ \slash SYD-2 determines the size of dense projections in presynaptic active zones in \bionameC. elegans . . . . . . . . . . . 849
Ben Short Senescent cells have a case of the SADS ?? Ben Short T cells JAK up integrin activity . . . . ?? Ben Short p120 CLASPs microtubules to junctions ?? Ben Short Arp2\slash $3$-deficient cells share their problems . . . . . . . . . . . . . 867 Caitlin Sedwick Arnaud Echard: Adieu, ma soeur . . . . . 868 Joel A. Swanson The noodle defense . . . . . . . . . . . 871 Clifford P. Brangwynne Phase transitions and size scaling of membrane-less organelles . . . . . . . . 875 Nina Schweizer and Cristina Ferrás and David M. Kern and Elsa Logarinho and Iain M. Cheeseman and Helder Maiato Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis . . . . . . . . . 883 Xiaobo Bai and Jonathan R. Bowen and Tara K. Knox and Kaifeng Zhou and Manuela Pendziwiat and Gregor Kuhlenbäumer and Charles V. Sindelar and Elias T. Spiliotis Novel septin 9 repeat motifs altered in neuralgic amyotrophy bind and bundle microtubules . . . . . . . . . . . . . . 895 Congying Wu and Elizabeth M. Haynes and Sreeja B. Asokan and Jeremy M. Simon and Norman E. Sharpless and Albert S. Baldwin and Ian J. Davis and Gary L. Johnson and James E. Bear Loss of Arp2/3 induces an NF-$ \kappa $B-dependent, nonautonomous effect on chemotactic signaling . . . . . . . . . 907 Elena A. Minina and Lada H. Filonova and Kazutake Fukada and Eugene I. Savenkov and Vladimir Gogvadze and David Clapham and Victoria Sanchez-Vera and Maria F. Suarez and Boris Zhivotovsky and Geoffrey Daniel and Andrei Smertenko and Peter V. Bozhkov Autophagy and metacaspase determine the mode of cell death in plants . . . . . . 917 Eric C. Swanson and Benjamin Manning and Hong Zhang and Jeanne B. Lawrence Higher-order unfolding of satellite heterochromatin is a consistent and early event in cell senescence . . . . . 929 Mathijs Vleugel and Eelco Tromer and Manja Omerzu and Vincent Groenewold and Wilco Nijenhuis and Berend Snel and Geert J. P. L. Kops Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 promote accurate chromosome segregation . . . . 943 Gina V. Caldas and Keith F. DeLuca and Jennifer G. DeLuca KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity . . . . . . . . 957 Roland G. Heym and Dennis Zimmermann and Franziska T. Edelmann and Lars Israel and Zeynep Ökten and David R. Kovar and Dierk Niessing In vitro reconstitution of an mRNA-transport complex reveals mechanisms of assembly and motor activation . . . . . . . . . . . . . . . 971 Adam Kassan and Albert Herms and Andrea Fernández-Vidal and Marta Bosch and Nicole L. Schieber and Babu J. N. Reddy and Alba Fajardo and Mariona Gelabert-Baldrich and Francesc Tebar and Carlos Enrich and Steven P. Gross and Robert G. Parton and Albert Pol Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains 985 Alessio Montresor and Matteo Bolomini-Vittori and Lara Toffali and Barbara Rossi and Gabriela Constantin and Carlo Laudanna JAK tyrosine kinases promote hierarchical activation of Rho and Rap modules of integrin activation . . . . . 1003 Michael J. Ophir and Beiyun C. Liu and Stephen C. Bunnell The N terminus of SKAP55 enables T cell adhesion to TCR and integrin ligands via distinct mechanisms . . . . . . . . . . 1021 Marta N. Shahbazi and Diego Megias and Carolina Epifano and Anna Akhmanova and Gregg G. Gundersen and Elaine Fuchs and Mirna Perez-Moreno CLASP2 interacts with p120-catenin and governs microtubule dynamics at adherens junctions . . . . . . . . . . . . . . . 1043 Pedro Monteiro and Carine Rossé and Antonio Castro-Castro and Marie Irondelle and Emilie Lagoutte and Perrine Paul-Gilloteaux and Claire Desnos and Etienne Formstecher and François Darchen and David Perrais and Alexis Gautreau and Maud Hertzog and Philippe Chavrier Endosomal WASH and exocyst complexes control exocytosis of MT1-MMP at invadopodia . . . . . . . . . . . . . . 1063 Akriti Prashar and Sonam Bhatia and Darren Gigliozzi and Tonya Martin and Carla Duncan and Cyril Guyard and Mauricio R. Terebiznik Filamentous morphology of bacteria delays the timing of phagosome morphogenesis in macrophages . . . . . . 1081
Mitch Leslie Adducin-1 moonlights at the mitotic spindle . . . . . . . . . . . . . . . . ?? Mitch Leslie Histone shortages hold back replication forks . . . . . . . . . . . . . . . . . ?? Mitch Leslie Thyroid hormones speed cellular aging ?? Mitch Leslie Sending sisters their separate ways . . 3 Caitlin Sedwick Michael Kozlov: a twist in membrane physics . . . . . . . . . . . . . . . . 4 Juan S. Bonifacino Adaptor proteins involved in polarized sorting . . . . . . . . . . . . . . . . 7 Po-Chao Chan and Rosaline Y. C. Hsu and Chih-Wei Liu and Chien-Chen Lai and Hong-Chen Chen Adducin-1 is essential for mitotic spindle assembly through its interaction with myosin--X . . . . . . . . . . . . . 19 Jakob Mejlvang and Yunpeng Feng and Constance Alabert and Kai J. Neelsen and Zuzana Jasencakova and Xiaobei Zhao and Michael Lees and Albin Sandelin and Philippe Pasero and Massimo Lopes and Anja Groth New histone supply regulates replication fork speed and PCNA unloading . . . . . 29 Susanne M. Germann and Vera Schramke and Rune Troelsgaard Pedersen and Irene Gallina and Nadine Eckert-Boulet and Vibe H. Oestergaard and Michael Lisby TopBP1\slash Dpb11 binds DNA anaphase bridges to prevent genome instability 45 He Zhang and Michael J. Zeitz and Hong Wang and Beibei Niu and Shengfang Ge and Wei Li and Jiuwei Cui and Guanjun Wang and Guanxiang Qian and Michael J. Higgins and Xianqun Fan and Andrew R. Hoffman and Ji-Fan Hu Long noncoding RNA-mediated intrachromosomal interactions promote imprinting at the Kcnq1 locus . . . . . 61 Victoria Bulat and Melanie Rast and Jan Pielage Presynaptic CK2 promotes synapse organization and stability by targeting Ankyrin2 . . . . . . . . . . . . . . . . 77 Katie L. Vermillion and Kevin A. Lidberg and Laura S. Gammill Cytoplasmic protein methylation is essential for neural crest migration . . 95 Ceniz Zihni and Peter M. G. Munro and Ahmed Elbediwy and Nicholas H. Keep and Stephen J. Terry and John Harris and Maria S. Balda and Karl Matter Dbl3 drives Cdc42 signaling at the apical margin to regulate junction position and apical differentiation . . 111 Alberto Zambrano and Verónica García-Carpizo and María Esther Gallardo and Raquel Villamuera and Maria Ana Gómez-Ferrería and Angel Pascual and Nicolas Buisine and Laurent M. Sachs and Rafael Garesse and Ana Aranda The thyroid hormone receptor $ \beta $ induces DNA damage and premature senescence . . . . . . . . . . . . . . . 129
Mitch Leslie Double-checking DNA synthesis . . . . . ?? Mitch Leslie NEMO surfaces in NF-$ \kappa $B-activating clusters . . . . . . . . . ?? Mitch Leslie The sources of cell stickiness . . . . . ?? Mitch Leslie Clearing the way for cancer cells . . . 149 Caitlin Sedwick Jonathon Howard: Motor proteins go walkabout . . . . . . . . . . . . . . . 150 Uri Weissbein and Nissim Benvenisty and Uri Ben-David Genome maintenance in pluripotent stem cells . . . . . . . . . . . . . . . . . 153 Maria M. Magiera and Elisabeth Gueydon and Etienne Schwob DNA replication and spindle checkpoints cooperate during S phase to delay mitosis and preserve genome integrity 165 Boubou Diagouraga and Alexei Grichine and Arnold Fertin and Jin Wang and Saadi Khochbin and Karin Sadoul Motor-driven marginal band coiling promotes cell shape change during platelet activation . . . . . . . . . . 177 Sebastian Kötter and Andreas Unger and Nazha Hamdani and Patrick Lang and Matthias Vorgerd and Luitgard Nagel-Steger and Wolfgang A. Linke Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins . . . . . . . . . . 187 Fenglan Dong and Kyosuke Shinohara and Yanick Botilde and Ryo Nabeshima and Yasuko Asai and Akemi Fukumoto and Toshiaki Hasegawa and Moe Matsuo and Hiroyuki Takeda and Hidetaka Shiratori and Tetsuya Nakamura and Hiroshi Hamada Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm . . . . . . . . . . . . . . . . . 203 Tetsuo Kobayashi and Sehyun Kim and Yu-Chun Lin and Takanari Inoue and Brian David Dynlacht The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly . . . . . . . . 215 Nadine Tarantino and Jean-Yves Tinevez and Elizabeth Faris Crowell and Bertrand Boisson and Ricardo Henriques and Musa Mhlanga and Fabrice Agou and Alain Israël and Emmanuel Laplantine TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO--IKK supramolecular structures . . . . . . . 231 Christine Jean and Xiao Lei Chen and Ju-Ock Nam and Isabelle Tancioni and Sean Uryu and Christine Lawson and Kristy K. Ward and Colin T. Walsh and Nichol L. G. Miller and Majid Ghassemian and Patric Turowski and Elisabetta Dejana and Sara Weis and David A. Cheresh and David D. Schlaepfer Inhibition of endothelial FAK activity prevents tumor metastasis by enhancing barrier function . . . . . . . . . . . . 247 Christopher P. Toret and Michael V. D'Ambrosio and Ronald D. Vale and Michael A. Simon and W. James Nelson A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell--cell adhesion 265
Ben Short CAL1 builds centromeres on the fly . . . ?? Ben Short A mutual interest in prostaglandin signaling . . . . . . . . . . . . . . . ?? Ben Short Prion infections come with strings attached . . . . . . . . . . . . . . . . ?? Ben Short KIF13B erects an endocytic scaffold . . 283 Caitlin Sedwick Ottoline Leyser: The beauty of plant genetics . . . . . . . . . . . . . . . . 284 Isabel M. Palacios Hop-on hop-off: Polysomes take a tour of the cell on endosomes . . . . . . . . . 287 Laura C. Kelley and Lauren L. Lohmer and Elliott J. Hagedorn and David R. Sherwood Traversing the basement membrane in vivo: a diversity of strategies . . . . 291 Valentina Debattisti and Diana Pendin and Elena Ziviani and Andrea Daga and Luca Scorrano Reduction of endoplasmic reticulum stress attenuates the defects caused by \bionameDrosophila mitofusin depletion 303 Chin-Chi Chen and Mekonnen Lemma Dechassa and Emily Bettini and Mary B. Ledoux and Christian Belisario and Patrick Heun and Karolin Luger and Barbara G. Mellone CAL1 is the \bionameDrosophila CENP--A assembly factor . . . . . . . . . . . . 313 Takatoshi Iijima and Yoko Iijima and Harald Witte and Peter Scheiffele Neuronal cell type--specific alternative splicing is regulated by the KH domain protein SLM1 . . . . . . . . . . . . . . 331 Yujiro Higuchi and Peter Ashwin and Yvonne Roger and Gero Steinberg Early endosome motility spatially organizes polysome distribution . . . . 343 Jessica Zapata and Noah Dephoure and Tracy MacDonough and Yaxin Yu and Emily J. Parnell and Meghan Mooring and Steven P. Gygi and David J. Stillman and Douglas R. Kellogg PP2A$^{\rm Rts1}$ is a master regulator of pathways that control cell size . . . 359 Chantal Binda and Samuel Génier and Andréane Cartier and Jean-François Larrivée and Jana Stankova and Jason C. Young and Jean-Luc Parent A G protein--coupled receptor and the intracellular synthase of its agonist functionally cooperate . . . . . . . . . 377 Yoshimitsu Kanai and Daliang Wang and Nobutaka Hirokawa KIF13B enhances the endocytosis of LRP1 by recruiting LRP1 to caveolae . . . . . 395 Andreas Schaupp and Ola Sabet and Irina Dudanova and Marion Ponserre and Philippe Bastiaens and Rüdiger Klein The composition of EphB2 clusters determines the strength in the cellular repulsion response . . . . . . . . . . . 409 Alexander Rouvinski and Sharon Karniely and Maria Kounin and Sanaa Moussa and Miri D. Goldberg and Gabriela Warburg and Roman Lyakhovetsky and Dulce Papy-Garcia and Janine Kutzsche and Carsten Korth and George A. Carlson and Susan F. Godsave and Peter J. Peters and Katarina Luhr and Krister Kristensson and Albert Taraboulos Live imaging of prions reveals nascent PrP$^{\rm Sc}$ in cell-surface, raft-associated amyloid strings and webs 423 Denis Tsygankov and Colleen G. Bilancia and Eric A. Vitriol and Klaus M. Hahn and Mark Peifer and Timothy C. Elston CellGeo: a computational platform for the analysis of shape changes in cells with complex geometries . . . . . . . . 443
Mitch Leslie Kicking MiD51 out of the enzyme club . . ?? Mitch Leslie Polarity proteins duel in \bionameDrosophila . . . . . . . . . . . ?? Mitch Leslie Ndc1 catches the shuttle . . . . . . . . ?? Mitch Leslie Career guidance for stem cells . . . . . 463 Caitlin Sedwick Amy Gladfelter: Fungi with a streak of individuality . . . . . . . . . . . . . 464 Jens Lykke-Andersen and Eric J. Bennett Protecting the proteome: Eukaryotic cotranslational quality control pathways 467 Viviane Richter and Catherine S. Palmer and Laura D. Osellame and Abeer P. Singh and Kirstin Elgass and David A. Stroud and Hiromi Sesaki and Marc Kvansakul and Michael T. Ryan Structural and functional analysis of MiD51, a dynamin receptor required for mitochondrial fission . . . . . . . . . 477 Clémence L. Gamblin and Émilie J.-L. Hardy and François J.-M. Chartier and Nicolas Bisson and Patrick Laprise A bidirectional antagonism between aPKC and Yurt regulates epithelial cell polarity . . . . . . . . . . . . . . . . 487 Douwe M. Veltman and Michael G. Lemieux and David A. Knecht and Robert H. Insall PIP$_3$-dependent macropinocytosis is incompatible with chemotaxis . . . . . . 497 Vincent Gaggioli and Eva Zeiser and David Rivers and Charles R. Bradshaw and Julie Ahringer and Philip Zegerman CDK phosphorylation of SLD-2 is required for replication initiation and germline development in \bionameC. elegans . . . 507 Jingjing Chen and Christine J. Smoyer and Brian D. Slaughter and Jay R. Unruh and Sue L. Jaspersen The SUN protein Mps3 controls Ndc1 distribution and function on the nuclear membrane . . . . . . . . . . . . . . . . 523 Taras Y. Nazarko and Katharine Ozeki and Andreas Till and Geetha Ramakrishnan and Pouya Lotfi and Mingda Yan and Suresh Subramani Peroxisomal Atg37 binds Atg30 or palmitoyl--CoA to regulate phagophore formation during pexophagy . . . . . . . 541 Alex J. Smith and Byung-Ju Jin and Julien Ratelade and Alan S. Verkman Aggregation state determines the localization and function of M1- and M23--aquaporin-4 in astrocytes . . . . . 559 Sérgio de Matos Simões and Avantika Mainieri and Jennifer A. Zallen Rho GTPase and Shroom direct planar polarized actomyosin contractility during convergent extension . . . . . . 575 Gwenvael Le Dréau and Murielle Saade and Irene Gutiérrez-Vallejo and Elisa Martí The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord 591 Sho Morioka and Peter Broglie and Emily Omori and Yuka Ikeda and Giichi Takaesu and Kunihiro Matsumoto and Jun Ninomiya-Tsuji TAK1 kinase switches cell fate from apoptosis to necrosis following TNF stimulation . . . . . . . . . . . . . . 607
Ben Short BUB-1 makes kinetochores MAD . . . . . . ?? Ben Short Lamin-A provides stiff resistance to cell migration . . . . . . . . . . . . . ?? Ben Short A surprising Twist to cell dissemination ?? Ben Short Mdm2 pulls the plug on glycolysis . . . 627 Caitlin Sedwick Feroz Papa: Saving cells from themselves 628 Takashi Ishikawa Protein tagging reveals new insights into signaling in flagella . . . . . . . 631 Albert Pol and Steven P. Gross and Robert G. Parton Biogenesis of the multifunctional lipid droplet: Lipids, proteins, and sites . . 635 Mark W. Moyle and Taekyung Kim and Neil Hattersley and Julien Espeut and Dhanya K. Cheerambathur and Karen Oegema and Arshad Desai A Bub1--Mad1 interaction targets the Mad1--Mad2 complex to unattached kinetochores to initiate the spindle checkpoint . . . . . . . . . . . . . . . 647 K\`evin Knoops and Selvambigai Manivannan and Ma\lgorzata N. Cepi\'nska and Arjen M. Krikken and Anita M. Kram and Marten Veenhuis and Ida J. van der Klei Preperoxisomal vesicles can form in the absence of Pex3 . . . . . . . . . . . . 659 Takamasa Harada and Joe Swift and Jerome Irianto and Jae-Won Shin and Kyle R. Spinler and Avathamsa Athirasala and Rocky Diegmiller and P. C. Dave P. Dingal and Irena L. Ivanovska and Dennis E. Discher Nuclear lamin stiffness is a barrier to $3$D migration, but softness can limit survival . . . . . . . . . . . . . . . . 669 Tsuyoshi Morita and Ken'ichiro Hayashi Arp5 is a key regulator of myocardin in smooth muscle cells . . . . . . . . . . 683 Debora Keller and Meritxell Orpinell and Nicolas Olivier and Malte Wachsmuth and Robert Mahen and Romain Wyss and Virginie Hachet and Jan Ellenberg and Suliana Manley and Pierre Gönczy Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells . . . 697 Pietri Puustinen and Anna Rytter and Monika Mortensen and Pekka Kohonen and José M. Moreira and Marja Jäättelä CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation 713 Takumi Mikawa and Takeshi Maruyama and Koji Okamoto and Hitoshi Nakagama and Matilde E. Lleonart and Takeshi Tsusaka and Kousuke Hori and Itsuo Murakami and Taisuke Izumi and Akifumi Takaori-Kondo and Masayuki Yokode and Gordon Peters and David Beach and Hiroshi Kondoh Senescence-inducing stress promotes proteolysis of phosphoglycerate mutase via ubiquitin ligase Mdm2 . . . . . . . 729 Shenhav Cohen and Donghoon Lee and Bo Zhai and Steven P. Gygi and Alfred L. Goldberg Trim32 reduces PI3K--Akt--FoxO signaling in muscle atrophy by promoting plakoglobin--PI3K dissociation . . . . . 747 Tony Cijsouw and Jens P. Weber and Jurjen H. Broeke and Jantine A. C. Broek and Desiree Schut and Tim Kroon and Ingrid Saarloos and Matthijs Verhage and Ruud F. Toonen Munc18-1 redistributes in nerve terminals in an activity- and PKC-dependent manner . . . . . . . . . . 759 Nicholas Ariotti and Manuel A. Fernández-Rojo and Yong Zhou and Michelle M. Hill and Travis L. Rodkey and Kerry L. Inder and Lukas B. Tanner and Markus R. Wenk and John F. Hancock and Robert G. Parton Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling . . . . . 777 Richard J. Chi and Jingxuan Liu and Matthew West and Jing Wang and Greg Odorizzi and Christopher G. Burd Fission of SNX--BAR--coated endosomal retrograde transport carriers is promoted by the dynamin-related protein Vps1 . . . . . . . . . . . . . . . . . . 793 Toshiyuki Oda and Haruaki Yanagisawa and Toshiki Yagi and Masahide Kikkawa Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity . . . . . . . . 807 Emily Steed and Ahmed Elbediwy and Barbara Vacca and Sébastien Dupasquier and Sandra A. Hemkemeyer and Tesha Suddason and Ana C. Costa and Jean-Bernard Beaudry and Ceniz Zihni and Ewen Gallagher and Christophe E. Pierreux and Maria S. Balda and Karl Matter MarvelD3 couples tight junctions to the MEKK1--JNK pathway to regulate cell behavior and survival . . . . . . . . . 821 Eliah R. Shamir and Elisa Pappalardo and Danielle M. Jorgens and Kester Coutinho and Wen-Ting Tsai and Khaled Aziz and Manfred Auer and Phuoc T. Tran and Joel S. Bader and Andrew J. Ewald Twist1-induced dissemination preserves epithelial identity and requires E-cadherin . . . . . . . . . . . . . . . 839
Ben Short Circumventing the Greatwall . . . . . . ?? Ben Short OPA1's shortcut to mitochondrial fission ?? Ben Short Tracking traction in amoeboid cells . . ?? Ben Short Motor proteins Hook on to early endosomes . . . . . . . . . . . . . . . 859 Caitlin Sedwick Yves Barral: Lessons from yeast on growth, renewal, and old age . . . . . . 860 Robert Walters and Roy Parker Is there quality control of localized mRNAs? . . . . . . . . . . . . . . . . . 863 Annamaria Ruggiano and Ombretta Foresti and Pedro Carvalho ER-associated degradation: Protein quality control and beyond . . . . . . . 869 Eiichi Okumura and Atsushi Morita and Mizuho Wakai and Satoru Mochida and Masatoshi Hara and Takeo Kishimoto Cyclin B--Cdk1 inhibits protein phosphatase PP2A--B55 via a Greatwall kinase--independent mechanism . . . . . 881 Ibtissem Nabti and Petros Marangos and Jenny Bormann and Nobuaki R. Kudo and John Carroll Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis I progression and fidelity . . . . . . . . 891 Edward R. Ballister and Michelle Riegman and Michael A. Lampson Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint . . . . . . . . . 901 Batool Ossareh-Nazari and Carlos A. Niño and Mario H. Bengtson and Joong-Won Lee and Claudio A. P. Joazeiro and Catherine Dargemont Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy 909 Ruchika Anand and Timothy Wai and Michael J. Baker and Nikolay Kladt and Astrid C. Schauss and Elena Rugarli and Thomas Langer The i -AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission . . . . . . . . . . . 919 Umut Sahin and Omar Ferhi and Marion Jeanne and Shirine Benhenda and Caroline Berthier and Florence Jollivet and Michiko Niwa-Kawakita and Orestis Faklaris and Niclas Setterblad and Hugues de Thé and Valérie Lallemand-Breitenbach Oxidative stress--induced assembly of PML nuclear bodies controls sumoylation of partner proteins . . . . . . . . . . 931 Budhaditya Banerjee and Cortney A. Kestner and P. Todd Stukenberg EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B . . . . . . . . . . . . . . . . 947 Rania S. Rizk and Katherine A. DiScipio and Kathleen G. Proudfoot and Mohan L. Gupta The kinesin-8 Kip3 scales anaphase spindle length by suppression of midzone microtubule polymerization . . . . . . . 965 Matthew Akamatsu and Julien Berro and Kai-Ming Pu and Irene R. Tebbs and Thomas D. Pollard Cytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase . . . . . . . . 977 Ewa Bielska and Martin Schuster and Yvonne Roger and Adokiye Berepiki and Darren M. Soanes and Nicholas J. Talbot and Gero Steinberg Hook is an adapter that coordinates kinesin-3 and dynein cargo attachment on early endosomes . . . . . . . . . . . . 989 Jun Zhang and Rongde Qiu and Herbert N. Arst and Miguel A. Peñalva and Xin Xiang HookA is a novel dynein--early endosome linker critical for cargo movement in vivo . . . . . . . . . . . . . . . . . . 1009 Evgeny Zatulovskiy and Richard Tyson and Till Bretschneider and Robert R. Kay Bleb-driven chemotaxis of \bionameDictyostelium cells . . . . . . 1027 Effie Bastounis and Ruedi Meili and Begoña Álvarez-González and Joshua Francois and Juan C. del Álamo and Richard A. Firtel and Juan C. Lasheras Both contractile axial and lateral traction force dynamics drive amoeboid cell motility . . . . . . . . . . . . . 1045 Jung Ha Kim and Kabsun Kim and Bang Ung Youn and Jongwon Lee and Inyoung Kim and Hong-In Shin and Haruhiko Akiyama and Yongwon Choi and Nacksung Kim Kruppel-like factor 4 attenuates osteoblast formation, function, and cross talk with osteoclasts . . . . . . 1063 Vincent Gaggioli and Eva Zeiser and David Rivers and Charles R. Bradshaw and Julie Ahringer and Philip Zegerman CDK phosphorylation of SLD-2 is required for replication initiation and germline development in \bionameC. elegans . . . 1075
Ben Short SUN proteins melt the nuclear envelope ?? Ben Short Dynamin loss bulks up endocytosis . . . ?? Ben Short p73 helps developing sperm stick to the right path . . . . . . . . . . . . . . . ?? Ben Short p53 cuts off invading cells . . . . . . 1079 Caitlin Sedwick Melina Schuh: First comes the egg . . . 1080 Nikolaus Pfanner and Martin van der Laan and Paolo Amati and Roderick A. Capaldi and Amy A. Caudy and Agnieszka Chacinska and Manjula Darshi and Markus Deckers and Suzanne Hoppins and Tateo Icho and Stefan Jakobs and Jianguo Ji and Vera Kozjak-Pavlovic and Chris Meisinger and Paul R. Odgren and Sang Ki Park and Peter Rehling and Andreas S. Reichert and M. Saeed Sheikh and Susan S. Taylor and Nobuo Tsuchida and Alexander M. van der Bliek and Ida J. van der Klei and Jonathan S. Weissman and Benedikt Westermann and Jiping Zha and Walter Neupert and Jodi Nunnari Uniform nomenclature for the mitochondrial contact site and cristae organizing system . . . . . . . . . . . 1083 Zu-Hang Sheng Mitochondrial trafficking and anchoring in neurons: New insight and implications 1087 Yagmur Turgay and Lysie Champion and Csaba Balazs and Michael Held and Alberto Toso and Daniel W. Gerlich and Patrick Meraldi and Ulrike Kutay SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown . . . . . . . . . . . 1099 Emmanuel Gallaud and Renaud Caous and Aude Pascal and Franck Bazile and Jean-Philippe Gagné and Sébastien Huet and Guy G. Poirier and Denis Chrétien and Laurent Richard-Parpaillon and Régis Giet Ensconsin\slash Map7 promotes microtubule growth and centrosome separation in \bionameDrosophila neural stem cells . . . . . . . . . . . . . . . 1111 Madhurima Dhara and Antonio Yarzagaray and Yvonne Schwarz and Soumyajit Dutta and Chad Grabner and Paanteha K. Moghadam and Anneka Bost and Claudia Schirra and Jens Rettig and Kerstin Reim and Nils Brose and Ralf Mohrmann and Dieter Bruns Complexin synchronizes primed vesicle exocytosis and regulates fusion pore dynamics . . . . . . . . . . . . . . . . 1123 Jaroslaw Kasprowicz and Sabine Kuenen and Jef Swerts and Katarzyna Miskiewicz and Patrik Verstreken Dynamin photoinactivation blocks Clathrin and $ \alpha $-adaptin recruitment and induces bulk membrane retrieval . . . . . . . . . . . . . . . 1141 Shannon L. Ballard and Daniel L. Miller and Barry Ganetzky Retrograde neurotrophin signaling through Tollo regulates synaptic growth in \bionameDrosophila . . . . . . . . . 1157 Lena Holembowski and Daniela Kramer and Dietmar Riedel and Raffaella Sordella and Alice Nemajerova and Matthias Dobbelstein and Ute M. Moll TAp73 is essential for germ cell adhesion and maturation in testis . . . 1173 Shota Yamauchi and Yan Yan Hou and Alvin Kunyao Guo and Hiroaki Hirata and Wataru Nakajima and Ai Kia Yip and Cheng-han Yu and Ichiro Harada and Keng-Hwee Chiam and Yasuhiro Sawada and Nobuyuki Tanaka and Keiko Kawauchi p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion . . . . . . . . . 1191 Elliott J. Hagedorn and Laura C. Kelley and Kaleb M. Naegeli and Zheng Wang and Qiuyi Chi and David R. Sherwood ADF\slash cofilin promotes invadopodial membrane recycling during cell invasion in vivo . . . . . . . . . . . . . . . . 1209 Bradley A. Heller and Monica Ghidinelli and Jakob Voelkl and Steven Einheber and Ryan Smith and Ethan Grund and Grant Morahan and David Chandler and Luba Kalaydjieva and Filippo Giancotti and Rosalind H. King and Aniko Naray Fejes-Toth and Gerard Fejes-Toth and Maria Laura Feltri and Florian Lang and James L. Salzer Functionally distinct PI $3$-kinase pathways regulate myelination in the peripheral nervous system . . . . . . . 1219
Ben Short Tat substrates open the portal . . . . . ?? Ben Short Protein coat keeps axons buttoned up . . ?? Ben Short Probing PtdIns4 P 's localization . . . ?? Ben Short First remodel, then recycle . . . . . . 3 Caitlin Sedwick Matthew Freeman: The sharp end of rhomboids . . . . . . . . . . . . . . . 4 Stefano Vavassori and Andreas Mayer A new life for an old pump: V--ATPase and neurotransmitter release . . . . . . 7 Markus Babst Quality control at the plasma membrane: One mechanism does not fit all . . . . . 11 Dong Wang and Daniel Epstein and Ossama Khalaf and Sankaranarayanan Srinivasan and W. Ryan Williamson and Amir Fayyazuddin and Florante A. Quiocho and P. Robin Hiesinger Ca$^{2+}$-Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1 . . . . . . . . . . . . . . 21 Manuel Alonso Y. Adell and Georg F. Vogel and Mehrshad Pakdel and Martin Müller and Herbert Lindner and Michael W. Hess and David Teis Coordinated binding of Vps4 to ESCRT--III drives membrane neck constriction during MVB vesicle formation . . . . . . . . . . . . . . . 33 Cassie Aldridge and Xianyue Ma and Fabien Gerard and Kenneth Cline Substrate-gated docking of pore subunit Tha4 in the TatC cavity initiates Tat translocase assembly . . . . . . . . . . 51 Steven L. Jones and Farida Korobova and Tatyana Svitkina Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments 67 Dylan T. Burnette and Lin Shao and Carolyn Ott and Ana M. Pasapera and Robert S. Fischer and Michelle A. Baird and Christelle Der Loughian and Helene Delanoe-Ayari and Matthew J. Paszek and Michael W. Davidson and Eric Betzig and Jennifer Lippincott-Schwartz A contractile and counterbalancing adhesion system controls the $3$D shape of crawling cells . . . . . . . . . . . 83 C. Florian Bentzinger and Julia von Maltzahn and Nicolas A. Dumont and Danny A. Stark and Yu Xin Wang and Kevin Nhan and Jérôme Frenette and D. D. W. Cornelison and Michael A. Rudnicki Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength . . . . . . . . 97 Gerald R. V. Hammond and Matthias P. Machner and Tamas Balla A novel probe for phosphatidylinositol $4$-phosphate reveals multiple pools beyond the Golgi . . . . . . . . . . . . 113
Mitch Leslie TRIM9 cuts back on axon branching . . . ?? Mitch Leslie Closing the calcium floodgates . . . . . ?? Mitch Leslie Talin holds tight during cell spreading ?? Mitch Leslie Not all vinculins are created equal . . 129 Caitlin Sedwick Alex Mogilner: Math illuminates biology 130 Emily Hatch and Martin Hetzer Breaching the nuclear envelope in development and disease . . . . . . . . 133 Lesley A. Kane and Michael Lazarou and Adam I. Fogel and Yan Li and Koji Yamano and Shireen A. Sarraf and Soojay Banerjee and Richard J. Youle PINK1 phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity . . . . . . . . . . . . . . . . 143 Ayelet Lesman and Jacob Notbohm and David A. Tirrell and Guruswami Ravichandran Contractile forces regulate cell division in three-dimensional environments . . . . . . . . . . . . . . 155 Julian Scherer and Julie Yi and Richard B. Vallee PKA-dependent dynein switching from lysosomes to adenovirus: a novel form of host--virus competition . . . . . . . . 163 Verónica Eisner and Guy Lenaers and György Hajnóczky Mitochondrial fusion is frequent in skeletal muscle and supports excitation--contraction coupling . . . . 179 Katharina Schneider and Eric Seemann and Lutz Liebmann and Rashmi Ahuja and Dennis Koch and Martin Westermann and Christian A. Hübner and Michael M. Kessels and Britta Qualmann ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function . . . 197 Cortney C. Winkle and Leslie M. McClain and Juli G. Valtschanoff and Charles S. Park and Christopher Maglione and Stephanie L. Gupton A novel Netrin-1--sensitive mechanism promotes local SNARE-mediated exocytosis during axon branching . . . . . . . . . 217 Pascal Béguin and Kazuaki Nagashima and Ramasubbu N. Mahalakshmi and Réjan Vigot and Atsuko Matsunaga and Takafumi Miki and Mei Yong Ng and Yu Jin Alvin Ng and Chiaw Hwee Lim and Hock Soon Tay and Le-Ann Hwang and Dmitri Firsov and Bor Luen Tang and Nobuya Inagaki and Yasuo Mori and Susumu Seino and Thomas Launey and Walter Hunziker BARP suppresses voltage-gated calcium channel activity and Ca$^{2+}$-evoked exocytosis . . . . . . . . . . . . . . . 233 Jennifer L. Bays and Xiao Peng and Catlin E. Tolbert and Christophe Guilluy and Ashley E. Angell and Yuan Pan and Richard Superfine and Keith Burridge and Kris A. DeMali Vinculin phosphorylation differentially regulates mechanotransduction at cell--cell and cell--matrix adhesions 251 Perrine Pinon and Jenita Pärssinen and Patricia Vazquez and Michael Bachmann and Rolle Rahikainen and Marie-Claude Jacquier and Latifeh Azizi and Juha A. Määttä and Martin Bastmeyer and Vesa P. Hytönen and Bernhard Wehrle-Haller Talin-bound NPLY motif recruits integrin-signaling adapters to regulate cell spreading and mechanosensing . . . 265
Ben Short Spindle microtubules sustain the tension ?? Ben Short The fellowship of the Rng . . . . . . . ?? Ben Short Tenascin-X and the pick of destiny . . . ?? Ben Short Clathrin's muscle-building regimen . . . 285 Caitlin Sedwick Carole Parent: Migrating cells relay the message . . . . . . . . . . . . . . . . 286 Brody Holohan and Woodring E. Wright and Jerry W. Shay Telomeropathies: an emerging spectrum disorder . . . . . . . . . . . . . . . . 289 Kim G. Lieu and Eun-Hee Shim and Jinling Wang and Ravi K. Lokareddy and Tao Tao and Gino Cingolani and Gerard P. Zambetti and David A. Jans The p53-induced factor Ei24 inhibits nuclear import through an importin $ \beta $-binding-like domain . . . . . . 301 Jeremy M. Chacón and Soumya Mukherjee and Breanna M. Schuster and Duncan J. Clarke and Melissa K. Gardner Pericentromere tension is self-regulated by spindle structure in metaphase . . . 313 Jianhua Huang and Daniel Kalderon Coupling of Hedgehog and Hippo pathways promotes stem cell maintenance by stimulating proliferation . . . . . . . 325 Eleni Petsalaki and George Zachos Chk2 prevents mitotic exit when the majority of kinetochores are unattached 339 Ning Wang and Libera Lo Presti and Yi-Hua Zhu and Minhee Kang and Zhengrong Wu and Sophie G. Martin and Jian-Qiu Wu The novel proteins Rng8 and Rng9 regulate the myosin--V Myo51 during fission yeast cytokinesis . . . . . . . 357 Stéphane Vassilopoulos and Christel Gentil and Jeanne Lainé and Pierre-Olivier Buclez and Agathe Franck and Arnaud Ferry and Guillaume Précigout and Robyn Roth and John E. Heuser and Frances M. Brodsky and Luis Garcia and Gis\`ele Bonne and Thomas Voit and France Piétri-Rouxel and Marc Bitoun Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization . . . . . . . . . 377 Alessandra Reversi and Eva Loeser and Devaraj Subramanian and Carsten Schultz and Stefano De Renzis Plasma membrane phosphoinositide balance regulates cell shape during \bionameDrosophila embryo morphogenesis 395 Lindsay B. Alcaraz and Jean-Yves Exposito and Nicolas Chuvin and Roxane M. Pommier and Caroline Cluzel and Sylvie Martel and Stéphanie Sentis and Laurent Bartholin and Claire Lethias and Ulrich Valcourt Tenascin-X promotes epithelial-to-mesenchymal transition by activating latent TGF-$ \beta $ . . . . 409
Ben Short Keeping DNA replication below PAR . . . ?? Ben Short Late endosomes uproot focal adhesions ?? Ben Short The spindle checkpoint's on-off switch ?? Ben Short Mitochondrial caspase keeps autophagy in flux . . . . . . . . . . . . . . . . . . 431 Caitlin Sedwick Peter Cullen: Nexins have it sorted . . 432 Koji Okamoto Organellophagy: Eliminating cellular building blocks via selective autophagy 435 Laura Benkemoun and Catherine Descoteaux and Nicolas T. Chartier and Lionel Pintard and Jean-Claude Labbé PAR-4\slash LKB1 regulates DNA replication during asynchronous division of the early \bionameC. elegans embryo 447 Dipen Rajgor and Jason A. Mellad and Daniel Soong and Jerome B. Rattner and Marvin J. Fritzler and Catherine M. Shanahan Mammalian microtubule P-body dynamics are mediated by nesprin-1 . . . . . . . 457 Lindsay DeVorkin and Nancy Erro Go and Ying-Chen Claire Hou and Annie Moradian and Gregg B. Morin and Sharon M. Gorski The \bionameDrosophila effector caspase Dcp-1 regulates mitochondrial dynamics and autophagic flux via SesB . . . . . . 477 Ilham A. Muslimov and Aliya Tuzhilin and Thean Hock Tang and Robert K. S. Wong and Riccardo Bianchi and Henri Tiedge Interactions of noncanonical motifs with hnRNP A2 promote activity-dependent RNA transport in neurons . . . . . . . . . . 493 Markus Hildenbeutel and Eric L. Hegg and Katharina Stephan and Steffi Gruschke and Brigitte Meunier and Martin Ott Assembly factors monitor sequential hemylation of cytochrome b to regulate mitochondrial translation . . . . . . . 511 Natalia Schiefermeier and Julia M. Scheffler and Mariana E. G. de Araujo and Taras Stasyk and Teodor Yordanov and Hannes L. Ebner and Martin Offterdinger and Sebastian Munck and Michael W. Hess and Sara A. Wickström and Anika Lange and Winfried Wunderlich and Reinhard Fässler and David Teis and Lukas A. Huber The late endosomal p14--MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration . . . . . 525 Daniel R. Matson and P. Todd Stukenberg CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status . . . . . 541 Caroline Funk and Verena Schmeiser and Jennifer Ortiz and Johannes Lechner A TOGL domain specifically targets yeast CLASP to kinetochores to stabilize kinetochore microtubules . . . . . . . . 555 Aleksandra Tata and David C. Stoppel and Shangyu Hong and Ayal Ben-Zvi and Tiao Xie and Chenghua Gu An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphorin 3E--PlexinD1 signaling . . . . . . . . . 573 Natalia H. Revelo and Dirk Kamin and Sven Truckenbrodt and Aaron B. Wong and Kirsten Reuter-Jessen and Ellen Reisinger and Tobias Moser and Silvio O. Rizzoli A new probe for super-resolution imaging of membranes elucidates trafficking pathways . . . . . . . . . . . . . . . . 591
Ben Short Talin's invasive side . . . . . . . . . ?? Ben Short Harvesting a new KASH crop . . . . . . . ?? Ben Short Endocytosis in its natural state . . . . ?? Ben Short CNTD1's crossover act . . . . . . . . . 609 Caitlin Sedwick Adam Cohen: Visualizing cellular voltage 610 Anthony K. L. Leung Poly(ADP-ribose): an organizer of cellular architecture . . . . . . . . . 613 David J. Erle and Dean Sheppard The cell biology of asthma . . . . . . . 621 J. Kim Holloway and Xianfei Sun and Rayka Yokoo and Anne M. Villeneuve and Paula E. Cohen Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites . . . . . . . 633 Soumitra Sau and Michael N. Conrad and Chih-Ying Lee and David B. Kaback and Michael E. Dresser and Makkuni Jayaram A selfish DNA element engages a meiosis-specific motor and telomeres for germ-line propagation . . . . . . . . . 643 Tyler J. Broering and Kris G. Alavattam and Ruslan I. Sadreyev and Yosuke Ichijima and Yasuko Kato and Kazuteru Hasegawa and R. Daniel Camerini-Otero and Jeannie T. Lee and Paul R. Andreassen and Satoshi H. Namekawa BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis . . . . . . . 663 Xiao Zhou and Katja Graumann and Lennart Wirthmueller and Jonathan D. G. Jones and Iris Meier Identification of unique SUN-interacting nuclear envelope proteins with diverse functions in plants . . . . . . . . . . 677 Ishu Saraogi and David Akopian and Shu-ou Shan Regulation of cargo recognition, commitment, and unloading drives cotranslational protein targeting . . . 693 Andreas Gerondopoulos and Ricardo Nunes Bastos and Shin-ichiro Yoshimura and Rachel Anderson and Sarah Carpanini and Irene Aligianis and Mark T. Handley and Francis A. Barr Rab18 and a Rab18 GEF complex are required for normal ER structure . . . . 707 Alexandre Grassart and Aaron T. Cheng and Sun Hae Hong and Fan Zhang and Nathan Zenzer and Yongmei Feng and David M. Briner and Gregory D. Davis and Dmitry Malkov and David G. Drubin Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis . . . . . . . . . . . . . . 721 Brian T. Beaty and Yarong Wang and Jose Javier Bravo-Cordero and Ved P. Sharma and Veronika Miskolci and Louis Hodgson and John Condeelis Talin regulates moesin--NHE--1 recruitment to invadopodia and promotes mammary tumor metastasis . . . . . . . . 737
Mitch Leslie Mps1 sends condensin II off to work . . ?? Mitch Leslie How sperm get into the zona . . . . . . ?? Mitch Leslie SPP pulls an inside job . . . . . . . . ?? Mitch Leslie A different angle on cell division . . . 755 Caitlin Sedwick Fernando Martín--Belmonte: Epithelia embrace the space . . . . . . . . . . . 756 William A. Prinz Bridging the gap: Membrane contact sites in signaling, metabolism, and organelle dynamics . . . . . . . . . . . . . . . . 759 Arnaud Obri and Munevver Parla Makinistoglu and Hong Zhang and Gerard Karsenty HDAC4 integrates PTH and sympathetic signaling in osteoblasts . . . . . . . . 771 Yuya Kagami and Keishi Nihira and Shota Wada and Masaya Ono and Mariko Honda and Kiyotsugu Yoshida Mps1 phosphorylation of condensin II controls chromosome condensation at the onset of mitosis . . . . . . . . . . . . 781 Mickael Machicoane and Cristina A. de Frutos and Jenny Fink and Murielle Rocancourt and Yannis Lombardi and Sonia Garel and Matthieu Piel and Arnaud Echard SLK-dependent activation of ERMs controls LGN--NuMA localization and spindle orientation . . . . . . . . . . 791 Matteo A. Avella and Boris Baibakov and Jurrien Dean A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans . . . . . 801 Jinhua Han and Ting Liu and Michael S. Y. Huen and Lin Hu and Zhiqiu Chen and Jun Huang SIVA1 directs the E3 ubiquitin ligase RAD18 for PCNA monoubiquitination . . . 811 Jackson Taylor and Andrea Pereyra and Tan Zhang and Maria Laura Messi and Zhong-Min Wang and Claudia Hereñú and Pei-Fen Kuan and Osvaldo Delbono The Ca$_v$ $ \beta_{1a}$ subunit regulates gene expression and suppresses myogenin in muscle progenitor cells . . 829 Jessica M. Boname and Stuart Bloor and Michal P. Wandel and James A. Nathan and Robin Antrobus and Kevin S. Dingwell and Teresa L. Thurston and Duncan L. Smith and James C. Smith and Felix Randow and Paul J. Lehner Cleavage by signal peptide peptidase is required for the degradation of selected tail-anchored proteins . . . . . . . . . 847
Ben Short Budding yeast do the Cdc42 two-step . . ?? Ben Short The fluid dynamics of collective cell migration . . . . . . . . . . . . . . . ?? Ben Short A short Anillin opens the way for germline development . . . . . . . . . . ?? Ben Short Defining the kinetochore's rules of engagement . . . . . . . . . . . . . . . 3 Caitlin Sedwick Sally Kornbluth: Nature's incredible contraptions . . . . . . . . . . . . . . 4 Anke Treuner-Lange and Lotte Sògaard-Andersen Regulation of cell polarity in bacteria 7 Pil Jung Kang and Mid Eum Lee and Hay-Oak Park Bud3 activates Cdc42 to establish a proper growth site in budding yeast . . 19 Michael Ensminger and Lucie Iloff and Christian Ebel and Teodora Nikolova and Bernd Kaina and Markus Löbrich DNA breaks and chromosomal aberrations arise when replication meets base excision repair . . . . . . . . . . . . 29 Anatoly V. Zaytsev and Lynsie J. R. Sundin and Keith F. DeLuca and Ekaterina L. Grishchuk and Jennifer G. DeLuca Accurate phosphoregulation of kinetochore--microtubule affinity requires unconstrained molecular interactions . . . . . . . . . . . . . . 45 Sergio A. Rincon and Payal Bhatia and Claudia Bicho and Merc\`e Guzman-Vendrell and Vincent Fraisier and Weronika E. Borek and Flavia de Lima Alves and Florent Dingli and Damarys Loew and Juri Rappsilber and Kenneth E. Sawin and Sophie G. Martin and Anne Paoletti Pom1 regulates the assembly of Cdr2--Mid1 cortical nodes for robust spatial control of cytokinesis . . . . . 61 Govind Kunduri and Changqing Yuan and Velayoudame Parthibane and Katherine M. Nyswaner and Ritu Kanwar and Kunio Nagashima and Steven G. Britt and Nickita Mehta and Varshika Kotu and Mindy Porterfield and Michael Tiemeyer and Patrick J. Dolph and Usha Acharya and Jairaj K. Acharya Phosphatidic acid phospholipase A1 mediates ER--Golgi transit of a family of G protein-coupled receptors . . . . . 79 Alexander Braun and Kyvan Dang and Felinah Buslig and Michelle A. Baird and Michael W. Davidson and Clare M. Waterman and Kenneth A. Myers Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells . . . . . . . . . . . 97 Sei Kuriyama and Eric Theveneau and Alexandre Benedetto and Maddy Parsons and Masamitsu Tanaka and Guillaume Charras and Alexandre Kabla and Roberto Mayor In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity . . . . . . . . . . . . 113 Rana Amini and Eugénie Goupil and Sara Labella and Monique Zetka and Amy S. Maddox and Jean-Claude Labbé and Nicolas T. Chartier \bionameC. elegans Anillin proteins regulate intercellular bridge stability and germline syncytial organization . . 129
Ben Short Exploring the LINC to nuclear envelope spacing . . . . . . . . . . . . . . . . ?? Ben Short GAAC pathway limits autophagy to a light snack . . . . . . . . . . . . . . . . . ?? Ben Short Deubiquitination helps Rad18 grow more tolerant . . . . . . . . . . . . . . . . ?? Ben Short Lipids help epithelia stand tall . . . . 147 Caitlin Sedwick Gohta Goshima: Questing for answers on the mitotic spindle . . . . . . . . . . 148 Xi Huang and Lily Yeh Jan Targeting potassium channels in cancer 151 Natalie E. Cain and Erin C. Tapley and Kent L. McDonald and Benjamin M. Cain and Daniel A. Starr The SUN protein UNC-84 is required only in force-bearing cells to maintain nuclear envelope architecture . . . . . 163 Rui Chen and Yilong Zou and Dongxue Mao and Daxiao Sun and Guanguang Gao and Jingwen Shi and Xiaoqing Liu and Chen Zhu and Mingyu Yang and Wanlu Ye and Qianqian Hao and Ruiqiang Li and Li Yu The general amino acid control pathway regulates mTOR and autophagy during serum/glutamine starvation . . . . . . . 173 Michelle K. Zeman and Jia-Ren Lin and Raimundo Freire and Karlene A. Cimprich DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis . . . . . . . . . . . . . . 183 Imre Gaspar and Yanxun V. Yu and Sean L. Cotton and Dae-Hwan Kim and Anne Ephrussi and Michael A. Welte Klar ensures thermal robustness of oskar localization by restraining RNP motility 199 Haidong Huang and Yujing Li and Keith E. Szulwach and Guoqiang Zhang and Peng Jin and Dahua Chen AGO3 Slicer activity regulates mitochondria--nuage localization of Armitage and piRNA amplification . . . . 217 Vitali Sikirzhytski and Valentin Magidson and Jonathan B. Steinman and Jie He and Maël Le Berre and Irina Tikhonenko and Jeffrey G. Ault and Bruce F. McEwen and James K. Chen and Haixin Sui and Matthieu Piel and Tarun M. Kapoor and Alexey Khodjakov Direct kinetochore--spindle pole connections are not required for chromosome segregation . . . . . . . . . 231 Mary Williard Elting and Christina L. Hueschen and Dylan B. Udy and Sophie Dumont Force on spindle microtubule minus ends moves chromosomes . . . . . . . . . . . 245 Erich J. Kushner and Luke S. Ferro and Jie-Yu Liu and Jessica R. Durrant and Stephen L. Rogers and Andrew C. Dudley and Victoria L. Bautch Excess centrosomes disrupt endothelial cell migration via centrosome scattering 257 Meng He and Khadar M. Abdi and Vann Bennett Ankyrin-G palmitoylation and $ \beta $II-spectrin binding to phosphoinositide lipids drive lateral membrane assembly 273 Motiur Rahman and Niraj K. Nirala and Alka Singh and Lihua Julie Zhu and Kaori Taguchi and Takeshi Bamba and Eiichiro Fukusaki and Leslie M. Shaw and David G. Lambright and Jairaj K. Acharya and Usha R. Acharya \bionameDrosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase $ \beta $ and regulates complex V activity . . . . . . 289 Emanuela Frittoli and Andrea Palamidessi and Paola Marighetti and Stefano Confalonieri and Fabrizio Bianchi and Chiara Malinverno and Giovanni Mazzarol and Giuseppe Viale and Ines Martin-Padura and Massimilliano Garré and Dario Parazzoli and Valentina Mattei and Salvatore Cortellino and Giovanni Bertalot and Pier Paolo Di Fiore and Giorgio Scita A RAB5/RAB4 recycling circuitry induces a proteolytic invasive program and promotes tumor dissemination . . . . . . 307
Mitch Leslie Lipid droplets ensure their own consumption . . . . . . . . . . . . . . ?? Mitch Leslie Loss of protein remodels shape-shifting parasite . . . . . . . . . . . . . . . . ?? Mitch Leslie Paxillin brings peace to microtubules ?? Mitch Leslie CLAMPing down on microtubules in migratory cells . . . . . . . . . . . . 331 Caitlin Sedwick Buzz Baum: The art of cell shape . . . . 332 Mikael Simons and Thomas Misgeld and Martin Kerschensteiner A unified cell biological perspective on axon--myelin injury . . . . . . . . . . 335 Satoshi Ninagawa and Tetsuya Okada and Yoshiki Sumitomo and Yukiko Kamiya and Koichi Kato and Satoshi Horimoto and Tokiro Ishikawa and Shunichi Takeda and Tetsushi Sakuma and Takashi Yamamoto and Kazutoshi Mori EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step . . . . . . . . . . . . . 347 Chao-Wen Wang and Yu-Hsuan Miao and Yi-Shun Chang A sterol-enriched vacuolar microdomain mediates stationary phase lipophagy in budding yeast . . . . . . . . . . . . . 357 Michael E. Werner and Jennifer W. Mitchell and William Putzbach and Elizabeth Bacon and Sun K. Kim and Brian J. Mitchell Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1 . . . . . . . . . . . . . . 367 Polly Hayes and Vladimir Varga and Sofia Olego-Fernandez and Jack Sunter and Michael L. Ginger and Keith Gull Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology 377 Kara J. Helmke and Rebecca Heald TPX2 levels modulate meiotic spindle size and architecture in \bionameXenopus egg extracts . . . . . . . . . . . . . . 385 Nicholas O. Deakin and Christopher E. Turner Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration . . . 395 Paolo Armando Gagliardi and Laura di Blasio and Alberto Puliafito and Giorgio Seano and Roberto Sessa and Federica Chianale and Thomas Leung and Federico Bussolino and Luca Primo PDK1-mediated activation of MRCK$ \alpha $ regulates directional cell migration and lamellipodia retraction . . . . . . 415 Claudia G. Vasquez and Mike Tworoger and Adam C. Martin Dynamic myosin phosphorylation regulates contractile pulses and tissue integrity during epithelial morphogenesis . . . . 435
Ben Short MagT1 helps a glycosylase gain acceptance . . . . . . . . . . . . . . . ?? Ben Short Sperm's sensitive steering machinery . . ?? Ben Short Lasp brings a giant down to size . . . . ?? Ben Short Vps74 gives phosphatase directions . . . 453 Caitlin Sedwick Heidi McBride: Mitochondria are well connected . . . . . . . . . . . . . . . 454 Kevin D. Corbett and Arshad Desai A new piece in the kinetochore jigsaw puzzle . . . . . . . . . . . . . . . . . 457 Carsten Janke The tubulin code: Molecular components, readout mechanisms, and functions . . . 461 Lisa J. Edens and Daniel L. Levy cPKC regulates interphase nuclear size during \bionameXenopus development . . . 473 Yiying Cai and Yongqiang Deng and Florian Horenkamp and Karin M. Reinisch and Christopher G. Burd Sac1--Vps74 structure reveals a mechanism to terminate phosphoinositide signaling in the Golgi apparatus . . . . 485 Anar K. Murphy and Michael Fitzgerald and Teresa Ro and Jee Hyun Kim and Ariana I. Rabinowitsch and Dipanjan Chowdhury and Carl L. Schildkraut and James A. Borowiec Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery . . . . . . . . 493 Peter Hornung and Paulina Troc and Francesca Malvezzi and Michael Maier and Zuzana Demianova and Tomasz Zimniak and Gabriele Litos and Fabienne Lampert and Alexander Schleiffer and Matthias Brunner and Karl Mechtler and Franz Herzog and Thomas C. Marlovits and Stefan Westermann A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP--A . . . . . . . . . . . . . . . . 509 Natalia A. Cherepanova and Shiteshu Shrimal and Reid Gilmore Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins . . . . 525 Magdalena Pichlo and Stefanie Bungert-Plümke and Ingo Weyand and Reinhard Seifert and Wolfgang Bönigk and Timo Strünker and Nachiket Dilip Kashikar and Normann Goodwin and Astrid Müller and Heinz G. Körschen and Ursel Collienne and Patric Pelzer and Qui Van and Jörg Enderlein and Clementine Klemm and Eberhard Krause and Christian Trötschel and Ansgar Poetsch and Elisabeth Kremmer and U. Benjamin Kaupp High density and ligand affinity confer ultrasensitive signal detection by a guanylyl cyclase chemoreceptor . . . . . 541 Isabelle Fernandes and Frieder Schöck The nebulin repeat protein Lasp regulates I-band architecture and filament spacing in myofibrils . . . . . 559
Ben Short If it ain't broke, don't fix it . . . . ?? Ben Short A replication origin gets SNP'd out in fragile X syndrome . . . . . . . . . . . ?? Ben Short Sphingomyelin is the master of its domain . . . . . . . . . . . . . . . . . ?? Ben Short Netrin puts an end to the anchor cell's vacillations . . . . . . . . . . . . . . 575 Caitlin Sedwick Elizabeth Chen: Fusing cells press closer . . . . . . . . . . . . . . . . . 576 Jeffrey A. Toretsky and Peter E. Wright Assemblages: Functional units formed by cellular phase separation . . . . . . . 579 Salim Ziani and Zita Nagy and Sergey Alekseev and Evi Soutoglou and Jean-Marc Egly and Frédéric Coin Sequential and ordered assembly of a large DNA repair complex on undamaged chromatin . . . . . . . . . . . . . . . 589 Jeannine Gerhardt and Nikica Zaninovic and Qiansheng Zhan and Advaitha Madireddy and Sarah L. Nolin and Nicole Ersalesi and Zi Yan and Zev Rosenwaks and Carl L. Schildkraut Cis-acting DNA sequence at a replication origin promotes repeat expansion to fragile X full mutation . . . . . . . . 599 Josse van Galen and Felix Campelo and Emma Martínez-Alonso and Margherita Scarpa and José Ángel Martínez-Menárguez and Vivek Malhotra Sphingomyelin homeostasis is required to form functional enzymatic domains at the trans-Golgi network . . . . . . . . . . 609 Zheng Wang and Lara M. Linden and Kaleb M. Naegeli and Joshua W. Ziel and Qiuyi Chi and Elliott J. Hagedorn and Natasha S. Savage and David R. Sherwood UNC-6 (netrin) stabilizes oscillatory clustering of the UNC-40 (DCC) receptor to orient polarity . . . . . . . . . . . 619 Christine Kienzle and Nirakar Basnet and Alvaro H. Crevenna and Gisela Beck and Bianca Habermann and Naoko Mizuno and Julia von Blume Cofilin recruits F-actin to SPCA1 and promotes Ca$^{2+}$-mediated secretory cargo sorting . . . . . . . . . . . . . 635 Ghazaleh Ashrafi and Julia S. Schlehe and Matthew J. LaVoie and Thomas L. Schwarz Mitophagy of damaged mitochondria occurs locally in distal neuronal axons and requires PINK1 and Parkin . . . . . . . 655 Laura Corrigan and Siamak Redhai and Aaron Leiblich and Shih-Jung Fan and Sumeth M. W. Perera and Rachel Patel and Carina Gandy and S. Mark Wainwright and John F. Morris and Freddie Hamdy and Deborah C. I. Goberdhan and Clive Wilson BMP-regulated exosomes from \bionameDrosophila male reproductive glands reprogram female behavior . . . . 671
Mitch Leslie Mitotic cells' shocking response to stress . . . . . . . . . . . . . . . . . ?? Mitch Leslie Sec12 heads for the exits . . . . . . . ?? Mitch Leslie ESCRT-III gets the bends . . . . . . . . ?? Mitch Leslie Topoisomerase II has to work late . . . 691 Caitlin Sedwick Margaret Robinson: Vesicles wear fancy coats . . . . . . . . . . . . . . . . . 692 Fred D. Mast and Alexander V. Ratushny and John D. Aitchison Systems cell biology . . . . . . . . . . 695 Mehdi Saadaoui and Mickaël Machicoane and Florencia di Pietro and Fred Etoc and Arnaud Echard and Xavier Morin Dlg1 controls planar spindle orientation in the neuroepithelium through direct interaction with LGN . . . . . . . . . . 707 Iris Titos and Tsvetomira Ivanova and Manuel Mendoza Chromosome length and perinuclear attachment constrain resolution of DNA intertwines . . . . . . . . . . . . . . 719 Alexandra N. Elsing and Camilla Aspelin and Johanna K. Björk and Heidi A. Bergman and Samu V. Himanen and Marko J. Kallio and Pia Roos-Mattjus and Lea Sistonen Expression of HSF2 decreases in mitosis to enable stress-inducible transcription and cell survival . . . . . . . . . . . 735 Kota Saito and Koh Yamashiro and Noriko Shimazu and Tomoya Tanabe and Kenji Kontani and Toshiaki Katada Concentration of Sec12 at ER exit sites via interaction with cTAGE5 is required for collagen export . . . . . . . . . . 751 Qing-Tao Shen and Amber L. Schuh and Yuqing Zheng and Kyle Quinney and Lei Wang and Michael Hanna and Julie C. Mitchell and Marisa S. Otegui and Paul Ahlquist and Qiang Cui and Anjon Audhya Structural analysis and modeling reveals new mechanisms governing ESCRT--III spiral filament assembly . . . . . . . . 763 Dipal M. Patel and Adi D. Dubash and Geri Kreitzer and Kathleen J. Green Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin--EB1 interactions . . . . . 779 Ying-Ting Zhu and Fu Li and Bo Han and Sean Tighe and Suzhen Zhang and Szu-Yu Chen and Xin Liu and Scheffer C. G. Tseng Activation of RhoA--ROCK--BMP signaling reprograms adult human corneal endothelial cells . . . . . . . . . . . 799
Mitch Leslie Enzyme helps fold the SAC . . . . . . . ?? Mitch Leslie HIV's unkind cut . . . . . . . . . . . . ?? Mitch Leslie DNA repair with a hefty price tag . . . ?? Mitch Leslie A storehouse for splicing proteins? . . 815 Caitlin Sedwick Laura Machesky: Actin opens the way . . 816 Ilia A. Droujinine and Dong Yan and Norbert Perrimon A sharp end to sugary Wingless travels 819 Jeremy Nance Getting to know your neighbor: Cell polarization in early embryos . . . . . 823 Antonio Espert and Pelin Uluocak and Ricardo Nunes Bastos and Davinderpreet Mangat and Philipp Graab and Ulrike Gruneberg PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing . . . . . 833 Deepak Adhikari and M. Kasim Diril and Kiran Busayavalasa and Sanjiv Risal and Shoma Nakagawa and Rebecca Lindkvist and Yan Shen and Vincenzo Coppola and Lino Tessarollo and Nobuaki R. Kudo and Philipp Kaldis and Kui Liu Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II . . . . . . . 843 Dong Kong and Veronica Farmer and Anil Shukla and Jana James and Richard Gruskin and Shigeo Kiriyama and Jadranka Loncarek Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles . . . . . . . . . . . . . . 855 Amy M. Sainski and Haiming Dai and Sekar Natesampillai and Yuan-Ping Pang and Gary D. Bren and Nathan W. Cummins and Cristina Correia and X. Wei Meng and James E. Tarara and Marina Ramirez-Alvarado and David J. Katzmann and Christina Ochsenbauer and John C. Kappes and Scott H. Kaufmann and Andrew D. Badley Casp8p41 generated by HIV protease kills CD4 T cells through direct Bak activation . . . . . . . . . . . . . . . 867 Olivia Barton and Steffen C. Naumann and Ronja Diemer-Biehs and Julia Künzel and Monika Steinlage and Sandro Conrad and Nodar Makharashvili and Jiadong Wang and Lin Feng and Bernard S. Lopez and Tanya T. Paull and Junjie Chen and Penny A. Jeggo and Markus Löbrich Polo-like kinase 3 regulates CtIP during DNA double-strand break repair in G1 . . 877 Sandy T. Oas and Anton L. Bryantsev and Richard M. Cripps Arrest is a regulator of fiber-specific alternative splicing in the indirect flight muscles of \bionameDrosophila . . 895 Georg Vogler and Jiandong Liu and Timothy W. Iafe and Ede Migh and József Mihály and Rolf Bodmer Cdc42 and formin activity control non-muscle myosin dynamics during \bionameDrosophila heart morphogenesis 909 Xiaoxi Wang and Andrea Page-McCaw A matrix metalloproteinase mediates long-distance attenuation of stem cell proliferation . . . . . . . . . . . . . 923
Ben Short A ribonuclease helps the ER get in shape ?? Ben Short Bem1p directs vesicular traffic . . . . ?? Ben Short Chibby's function in the dock . . . . . ?? Ben Short How mitosis keeps itself in order . . . 3 Caitlin Sedwick Raphael Valdivia: How \bionameChlamydia settles in . . . . . . . . . . . . . . . 4 Alan Hall Always aiming for the best: For scientists and for science . . . . . . . 7 Robert S. Fischer Move your microvilli . . . . . . . . . . 9 Maija Slaidina and Ruth Lehmann Translational control in germline stem cell development . . . . . . . . . . . . 13 Dan Lu and Jennifer Y. Hsiao and Norman E. Davey and Vanessa A. Van Voorhis and Scott A. Foster and Chao Tang and David O. Morgan Multiple mechanisms determine the order of APC/C substrate degradation in mitosis . . . . . . . . . . . . . . . . 23 Dianne S. Schwarz and Michael D. Blower The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation . . . . . 41 Dongmei Liu and Peter Novick Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p . . . . . . . . 59 Nah-Young Shin and Hyewon Choi and Lynn Neff and Yumei Wu and Hiroaki Saito and Shawn M. Ferguson and Pietro De Camilli and Roland Baron Dynamin and endocytosis are required for the fusion of osteoclasts and myoblasts 73 Chikara Tanaka and Li-Jing Tan and Keisuke Mochida and Hiromi Kirisako and Michiko Koizumi and Eri Asai and Machiko Sakoh-Nakatogawa and Yoshinori Ohsumi and Hitoshi Nakatogawa Hrr25 triggers selective autophagy--related pathways by phosphorylating receptor proteins . . . 91 Christoph Klingner and Anoop V. Cherian and Johannes Fels and Philipp M. Diesinger and Roland Aufschnaiter and Nicola Maghelli and Thomas Keil and Gisela Beck and Iva M. Toli\'c-Nòrrelykke and Mark Bathe and Roland Wedlich-Soldner Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells . . . . . . . . . . 107 Michael C. Burke and Feng-Qian Li and Benjamin Cyge and Takeshi Arashiro and Heather M. Brechbuhl and Xingwang Chen and Saul S. Siller and Matthew A. Weiss and Christopher B. O'Connell and Damon Love and Christopher J. Westlake and Susan D. Reynolds and Ryoko Kuriyama and Ken-Ichi Takemaru Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation . . . . . . 123 Sherry Dadgar and Zuyi Wang and Helen Johnston and Akanchha Kesari and Kanneboyina Nagaraju and Yi-Wen Chen and D. Ashley Hill and Terence A. Partridge and Mamta Giri and Robert J. Freishtat and Javad Nazarian and Jianhua Xuan and Yue Wang and Eric P. Hoffman Asynchronous remodeling is a driver of failed regeneration in Duchenne muscular dystrophy . . . . . . . . . . . . . . . 139 Amy M. Sainski and Haiming Dai and Sekar Natesampillai and Yuan-Ping Pang and Gary D. Bren and Nathan W. Cummins and Cristina Correia and X. Wei Meng and James E. Tarara and Marina Ramirez-Alvarado and David J. Katzmann and Christina Ochsenbauer and John C. Kappes and Scott H. Kaufmann and Andrew D. Badley Casp8p41 generated by HIV protease kills CD4 T cells through direct Bak activation . . . . . . . . . . . . . . . 159
Ben Short A new CLUH to mitochondrial biogenesis ?? Ben Short Septins provide a link to epithelial migration . . . . . . . . . . . . . . . ?? Ben Short Acinus cleavage cuts down on autophagy ?? Ben Short TGF-$ \beta $ gets primed for action . . 163 Caitlin Sedwick Guangshuo Ou: New perspectives on Q cell biology . . . . . . . . . . . . . . . . 164 Erik H. J. Danen Ignoring matrix boundaries when the LKB1 master kinase is gone . . . . . . . . . 167 Danelle Devenport The cell biology of planar cell polarity 171 Katerina Ragkousi and Matthew C. Gibson Cell division and the maintenance of epithelial order . . . . . . . . . . . . 181 Chloe E. Snider and Andrew D. Stephens and Jacob G. Kirkland and Omar Hamdani and Rohinton T. Kamakaka and Kerry Bloom Dyskerin, tRNA genes, and condensin tether pericentric chromatin to the spindle axis in mitosis . . . . . . . . 189 David Kachaner and Xavier Pinson and Khaled Ben El Kadhi and Karine Normandin and Lama Talje and Hugo Lavoie and Guillaume Lépine and Sébastien Carréno and Benjamin H. Kwok and Gilles R. Hickson and Vincent Archambault Interdomain allosteric regulation of Polo kinase by Aurora B and Map205 is required for cytokinesis . . . . . . . . 201 Jie Gao and Désirée Schatton and Paola Martinelli and Henriette Hansen and David Pla-Martin and Esther Barth and Christian Becker and Janine Altmueller and Peter Frommolt and Marco Sardiello and Elena I. Rugarli CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins . . . . . . . . . 213 Lee Dolat and John L. Hunyara and Jonathan R. Bowen and Eva Pauline Karasmanis and Maha Elgawly and Vitold E. Galkin and Elias T. Spiliotis Septins promote stress fiber-mediated maturation of focal adhesions and renal epithelial motility . . . . . . . . . . 225 Taesun Eom and Ilham A. Muslimov and Panayiotis Tsokas and Valerio Berardi and Jun Zhong and Todd C. Sacktor and Henri Tiedge Neuronal BC RNAs cooperate with eIF4B to mediate activity-dependent translational control . . . . . . . . . . . . . . . . 237 Nilay Nandi and Lauren K. Tyra and Drew Stenesen and Helmut Krämer Acinus integrates AKT1 and subapoptotic caspase activities to regulate basal autophagy . . . . . . . . . . . . . . . 253 Michael Taschner and Fruzsina Kotsis and Philipp Braeuer and E. Wolfgang Kuehn and Esben Lorentzen Crystal structures of IFT70/52 and IFT52/46 provide insight into intraflagellar transport B core complex assembly . . . . . . . . . . . . . . . . 269 Franco Klingberg and Melissa L. Chow and Anne Koehler and Stellar Boo and Lara Buscemi and Thomas M. Quinn and Mercedes Costell and Benjamin A. Alman and Elisabeth Genot and Boris Hinz Prestress in the extracellular matrix sensitizes latent TGF-$ \beta $1 for activation . . . . . . . . . . . . . . . 283 Keefe T. Chan and Sreeja B. Asokan and Samantha J. King and Tao Bo and Evan S. Dubose and Wenjin Liu and Matthew E. Berginski and Jeremy M. Simon and Ian J. Davis and Shawn M. Gomez and Norman E. Sharpless and James E. Bear LKB1 loss in melanoma disrupts directional migration toward extracellular matrix cues . . . . . . . 299
Ben Short Satellite RNA guides kinetochore assembly . . . . . . . . . . . . . . . . ?? Ben Short mTORC2 helps brown adipose tissue fuel up . . . . . . . . . . . . . . . . . . . ?? Ben Short Building modular motor complexes . . . . ?? Ben Short Red and green traffic signals . . . . . 319 Kendall Powell Dan Fletcher: a recipe for cooking up cellular machines . . . . . . . . . . . 320 Gary J. Brouhard and Luke M. Rice The contribution of $ \alpha \beta $-tubulin curvature to microtubule dynamics . . . . . . . . . . . . . . . . 323 Silvana Ro\vsi\'c and Florian Köhler and Sylvia Erhardt Repetitive centromeric satellite RNA is essential for kinetochore formation and cell division . . . . . . . . . . . . . 335 Lydie Couturier and Mateusz Trylinski and Khallil Mazouni and Léa Darnet and François Schweisguth A fluorescent tagging approach in \bionameDrosophila reveals late endosomal trafficking of Notch and Sanpodo . . . . . . . . . . . . . . . . 351 Jessica M. Olsen and Masaaki Sato and Olof S. Dallner and Anna L. Sandström and Didier F. Pisani and Jean-Claude Chambard and Ez-Zoubir Amri and Dana S. Hutchinson and Tore Bengtsson Glucose uptake in brown fat cells is dependent on mTOR complex $2$--promoted GLUT1 translocation . . . . . . . . . . 365 Oluwatobi Ogun and Marisa Zallocchi Clarin-1 acts as a modulator of mechanotransduction activity and presynaptic ribbon assembly . . . . . . 375 Stephen R. Norris and Virupakshi Soppina and Aslan S. Dizaji and Kristin I. Schimert and David Sept and Dawen Cai and Sivaraj Sivaramakrishnan and Kristen J. Verhey A method for multiprotein assembly in cells reveals independent action of kinesins in complex . . . . . . . . . . 393 Sara Löchte and Sharon Waichman and Oliver Beutel and Changjiang You and Jacob Piehler Live cell micropatterning reveals the dynamics of signaling complexes at the plasma membrane . . . . . . . . . . . . 407 Yi Shen and Morgane Rosendale and Robert E. Campbell and David Perrais pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis . . . . . . . . . . . . . . 419 Matthew J. Van Hook and Caitlyn M. Parmelee and Minghui Chen and Karlene M. Cork and Carina Curto and Wallace B. Thoreson Calmodulin enhances ribbon replenishment and shapes filtering of synaptic transmission by cone photoreceptors . . ??
Mitch Leslie Wait! Don't throw away those proteins! ?? Mitch Leslie DDR1 key for cancer cell escape . . . . ?? Mitch Leslie Melanoma cells travel on calcium cycles ?? Mitch Leslie A molecular chain gang at work in maturing ribosomes . . . . . . . . . . . 435 Caitlin Sedwick Valentina Greco: Got hair? . . . . . . . 436 Scott W. Crawley and Mark S. Mooseker and Matthew J. Tyska Shaping the intestinal brush border . . 441 Ana Clara Fernandes and Valerie Uytterhoeven and Sabine Kuenen and Yu-Chun Wang and Jan R. Slabbaert and Jef Swerts and Jaroslaw Kasprowicz and Stein Aerts and Patrik Verstreken Reduced synaptic vesicle protein degradation at lysosomes curbs TBC1D24/sky-induced neurodegeneration 453 Jonathan Bizarro and Christophe Charron and Séverine Boulon and Belinda Westman and Béreng\`ere Pradet-Balade and Franck Vandermoere and Marie-Eve Chagot and Marie Hallais and Yasmeen Ahmad and Heinrich Leonhardt and Angus Lamond and Xavier Manival and Christiane Branlant and Bruno Charpentier and Céline Verheggen and Edouard Bertrand Proteomic and $3$D structure analyses highlight the C/D box snoRNP assembly mechanism and its control . . . . . . . 463 Jochen Baßler and Helge Paternoga and Iris Holdermann and Matthias Thoms and Sander Granneman and Clara Barrio-Garcia and Afua Nyarko and Woonghee Lee and Gunter Stier and Sarah A. Clark and Daniel Schraivogel and Martina Kallas and Roland Beckmann and David Tollervey and Elisar Barbar and Irmi Sinning and Ed Hurt A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation . . . . . 481 Laura A. Jones and Cécile Villemant and Toby Starborg and Anna Salter and Georgina Goddard and Peter Ruane and Philip G. Woodman and Nancy Papalopulu and Sarah Woolner and Victoria J. Allan Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion . . . 499 Amélie Juin and Julie Di Martino and Birgit Leitinger and Elodie Henriet and Anne-Sophie Gary and Lisa Paysan and Jeremy Bomo and Georges Baffet and Cécile Gauthier-Rouvi\`ere and Jean Rosenbaum and Violaine Moreau and Frédéric Saltel Discoidin domain receptor 1 controls linear invadosome formation via a Cdc42--Tuba pathway . . . . . . . . . . 517 Jianwei Sun and Fujian Lu and Huifang He and Junling Shen and Jane Messina and Rahel Mathew and Dapeng Wang and Amod A. Sarnaik and Wei-Chiao Chang and Minjung Kim and Heping Cheng and Shengyu Yang STIM1- and Orai1-mediated Ca$^{2+}$ oscillation orchestrates invadopodium formation and melanoma invasion . . . . 535 Mohammed I. Ahmed and Majid Alam and Vladimir U. Emelianov and Krzysztof Poterlowicz and Ankit Patel and Andrey A. Sharov and Andrei N. Mardaryev and Natalia V. Botchkareva MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway . . . . . . 549 Joseph E. Chambers and Kseniya Petrova and Giulia Tomba and Michele Vendruscolo and David Ron ADP ribosylation adapts an ER chaperone response to short-term fluctuations in unfolded protein load . . . . . . . . . 569
Ben Short Macrophages show neutrophils the exit ?? Ben Short CDK5 opens the way for DLC1 . . . . . . ?? Ben Short PIP$_2$ directs vinculin assembly . . . ?? Ben Short Unlocking the secrets of chitinase secretion . . . . . . . . . . . . . . . 573 Caitlin Sedwick Miklós Müller: The deep history of eukaryotic metabolism . . . . . . . . . 574 Christopher P. Toret and Caitlin Collins and W. James Nelson An Elmo--Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion . . . . . . . 577 Sebastien Tauzin and Taylor W. Starnes and Francisco Barros Becker and Pui-ying Lam and Anna Huttenlocher Redox and Src family kinase signaling control leukocyte wound attraction and neutrophil reverse migration . . . . . . 589 Marcel Reuter and Alex Zelensky and Ihor Smal and Erik Meijering and Wiggert A. van Cappellen and H. Martijn de Gruiter and Gijsbert J. van Belle and Martin E. van Royen and Adriaan B. Houtsmuller and Jeroen Essers and Roland Kanaar and Claire Wyman BRCA2 diffuses as oligomeric clusters with RAD51 and changes mobility after DNA damage in live cells . . . . . . . . 599 Jaeger J. Hamilton and Victoria L. Marlow and Richard A. Owen and Marília de Assis Alcoforado Costa and Manman Guo and Grant Buchanan and Govind Chandra and Matthias Trost and Sarah J. Coulthurst and Tracy Palmer and Nicola R. Stanley-Wall and Frank Sargent A holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens . . . . 615 Brajendra K. Tripathi and Xiaolan Qian and Philipp Mertins and Dunrui Wang and Alex G. Papageorge and Steven A. Carr and Douglas R. Lowy CDK5 is a major regulator of the tumor suppressor DLC1 . . . . . . . . . . . . 627 Krishna Chinthalapudi and Erumbi S. Rangarajan and Dipak N. Patil and Eric M. George and David T. Brown and Tina Izard Lipid binding promotes oligomerization and focal adhesion activity of vinculin 643 Pallavi Bhattaram and Alfredo Penzo-Méndez and Kenji Kato and Kaustav Bandyopadhyay and Abhilash Gadi and Makoto M. Taketo and Véronique Lefebvre SOXC proteins amplify canonical WNT signaling to secure nonchondrocytic fates in skeletogenesis . . . . . . . . 657 Silvana Ro\vsi\'c and Florian Köhler and Sylvia Erhardt Repetitive centromeric satellite RNA is essential for kinetochore formation and cell division . . . . . . . . . . . . . 673 Magdalena Pichlo and Stefanie Bungert-Plümke and Ingo Weyand and Reinhard Seifert and Wolfgang Bönigk and Timo Strünker and Nachiket Dilip Kashikar and Normann Goodwin and Astrid Müller and Heinz G. Körschen and Ursel Collienne and Patric Pelzer and Qui Van and Jörg Enderlein and Clementine Klemm and Eberhard Krause and Christian Trötschel and Ansgar Poetsch and Elisabeth Kremmer and U. Benjamin Kaupp High density and ligand affinity confer ultrasensitive signal detection by a guanylyl cyclase chemoreceptor . . . . . 675
Ben Short KIF4A knows its own limits . . . . . . . ?? Ben Short Understanding the DisAp-pearance of cilia orientation . . . . . . . . . . . ?? Ben Short Ankyrin aweigh for axonal transport . . ?? Ben Short Osmotic gradient is just the tonic for wounded epithelia . . . . . . . . . . . 679 Kendall Powell Dominique Bergmann: Passionate about plant polarity . . . . . . . . . . . . .